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The map label for this gene is ilvI [H]

Identifier: 110633180

GI number: 110633180

Start: 910514

End: 912187

Strand: Direct

Name: ilvI [H]

Synonym: Meso_0823

Alternate gene names: 110633180

Gene position: 910514-912187 (Clockwise)

Preceding gene: 110633179

Following gene: 110633181

Centisome position: 20.64

GC content: 66.91

Gene sequence:

>1674_bases
ATGAAACTCGAATCCATCACACGCCACGAGACCGTCGGCGATTTCATTGCAGCCTATCTTGCCGATATCGGCGTCGAGGT
GATTTTTGGCGTCATCTCCATCCACAACATGCCGATCCTCGACGCGATCGCCCGCCAGGGGCGGGTCCGGTTCATTCCGG
CGCGCGGCGAGGCGGGCGCCATGAACATGGCCGATGCCTATGCACGCGTATCGGGCAAGCTGGGCGTCGTGATGACCTCG
ACGGGTACGGCGGCGGGCAATGCCGCGGGCGCCCAGGTCGAGGCGCTGACCGCGGGCACGTCTCTGCTGCACATCACCTC
GACCGTCGACACCCAGTTCATGGACCGCGACCGCGCGCCGATCCACGACGTTCCGCGCCAGCCCGACATGCTCAAGGGCA
TCTCCAAGGCCTATTACCGTCTCTGGGATGCAAAGAGTGCCATCGGCACGCTGACCGCTGCCGTCACCGCGGCCATCAGC
GCGCCCACTGGGCCGGTCAGCCTTGAAATTCCGGTTGACGTCCAGCGCATGGCTGCGCTGCCCACGACGCGCGTCTTTGC
GCCGCAACCGCAGAGCCCCCGCGCCAACGACGCCGCGATCGAGGAGATCGCCCGGCTCGTTGCAGGGGCGCGGCGGCCCA
TGCTCTGGCTCGGGGGCGGCGCGCGCGGCGCCCGCAAGCAAGCGAGCGAACTGGTCGAGCGCGGCTTCGCCCTCGTCACC
TCCACCAATGGCCGCGCCACGGTTTCGGAGGACAATCCCGCCAATCTGGGCGCCTTCAACATGGCGCCCGAATCCCAAGC
CCTCTACAAGACCATCGACCTGATGATCGTCGTGGGCTCCAAGCTGCGGGGCAACGAGACGCTCAACAACACCATGCGCC
TGCCTCGGCCCACCATCCAGATCGACGCCGACGCCTCGCAGGGCGGACGCAACTATCCGGTGGATTTGTTCGTCCATGGC
GATGCCGCCGATACCCTCCAGCGCTTGCTCGATCGCCTGCCGCAAAAGCTTTCCGTCGATCCCAATCTTGTCTTCGACGT
CGCCCGTACCCGCGCGCAGGGGGAGGGGACGATGCGCGCCGCCATGGGTCCTTATGGCAGCCTCGCCGACGCGCTGCTCG
AACGCGTGGCGCCGGGCCGTCACCCGTGGGTGCGCGATGTGACTATCTCCAACTCCACCTTCGGCAACCGCTACGTCAAG
TTCGCCGAGCCCCGCCACGGCGTCCATGCGTTGGGCGGCGGCATCGGGCAGGGCATCGCCATGGGCATCGGCGCCGCCAT
CGCATCGCCGTCGGCCAAGGCGATCACGCTGCTGGGCGATGGGGGCGCCCAGCTCGGCATCGCAGAAATGATCACAGCGG
TCGAGGAGCGAGCACCGCTCGTCTACGTGCTCATGAACGACCAGGCCTATGGCGTGATCCGCAACATTCAGGACGCCCAG
TATAATAGCCGGCACCACTATTCCGCGCTTCGCACGCCTAACTTCGCCGCCCATTGCGCCTCCATCGGCTTGCCGCACCG
CAAGGTTACGGCGGTCGCCGATTTCGCCGACGCGCTCGACGCGGCGCTGGCGGCAGACGGTCCGCAGCTCATCGAAATCG
ACATGTTGGCGATCGGCCCCTTCGCCGAGAGCTTTTCCGGCCCCCCGGCCGGCGCCGCGGGCAATAAGGTCTGA

Upstream 100 bases:

>100_bases
TCTTGGGCTCTCACGCCGCCAGCTACATCACGGGCGCGCAACTCGATGTATCGGGCGGCCTCGCCCGGCACATCTGACAT
CCAGTTCATTGGAAGCCGAT

Downstream 100 bases:

>100_bases
CTGCCGTGCATCTGGGACTGATCGGCTACGGCAATATCGCTCGCACGCTGATGGGGATACTATCGCGCGAAAGCGTTCCG
CTCTCGCAGGTGACCGTCGT

Product: hypothetical protein

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS [H]

Number of amino acids: Translated: 557; Mature: 557

Protein sequence:

>557_residues
MKLESITRHETVGDFIAAYLADIGVEVIFGVISIHNMPILDAIARQGRVRFIPARGEAGAMNMADAYARVSGKLGVVMTS
TGTAAGNAAGAQVEALTAGTSLLHITSTVDTQFMDRDRAPIHDVPRQPDMLKGISKAYYRLWDAKSAIGTLTAAVTAAIS
APTGPVSLEIPVDVQRMAALPTTRVFAPQPQSPRANDAAIEEIARLVAGARRPMLWLGGGARGARKQASELVERGFALVT
STNGRATVSEDNPANLGAFNMAPESQALYKTIDLMIVVGSKLRGNETLNNTMRLPRPTIQIDADASQGGRNYPVDLFVHG
DAADTLQRLLDRLPQKLSVDPNLVFDVARTRAQGEGTMRAAMGPYGSLADALLERVAPGRHPWVRDVTISNSTFGNRYVK
FAEPRHGVHALGGGIGQGIAMGIGAAIASPSAKAITLLGDGGAQLGIAEMITAVEERAPLVYVLMNDQAYGVIRNIQDAQ
YNSRHHYSALRTPNFAAHCASIGLPHRKVTAVADFADALDAALAADGPQLIEIDMLAIGPFAESFSGPPAGAAGNKV

Sequences:

>Translated_557_residues
MKLESITRHETVGDFIAAYLADIGVEVIFGVISIHNMPILDAIARQGRVRFIPARGEAGAMNMADAYARVSGKLGVVMTS
TGTAAGNAAGAQVEALTAGTSLLHITSTVDTQFMDRDRAPIHDVPRQPDMLKGISKAYYRLWDAKSAIGTLTAAVTAAIS
APTGPVSLEIPVDVQRMAALPTTRVFAPQPQSPRANDAAIEEIARLVAGARRPMLWLGGGARGARKQASELVERGFALVT
STNGRATVSEDNPANLGAFNMAPESQALYKTIDLMIVVGSKLRGNETLNNTMRLPRPTIQIDADASQGGRNYPVDLFVHG
DAADTLQRLLDRLPQKLSVDPNLVFDVARTRAQGEGTMRAAMGPYGSLADALLERVAPGRHPWVRDVTISNSTFGNRYVK
FAEPRHGVHALGGGIGQGIAMGIGAAIASPSAKAITLLGDGGAQLGIAEMITAVEERAPLVYVLMNDQAYGVIRNIQDAQ
YNSRHHYSALRTPNFAAHCASIGLPHRKVTAVADFADALDAALAADGPQLIEIDMLAIGPFAESFSGPPAGAAGNKV
>Mature_557_residues
MKLESITRHETVGDFIAAYLADIGVEVIFGVISIHNMPILDAIARQGRVRFIPARGEAGAMNMADAYARVSGKLGVVMTS
TGTAAGNAAGAQVEALTAGTSLLHITSTVDTQFMDRDRAPIHDVPRQPDMLKGISKAYYRLWDAKSAIGTLTAAVTAAIS
APTGPVSLEIPVDVQRMAALPTTRVFAPQPQSPRANDAAIEEIARLVAGARRPMLWLGGGARGARKQASELVERGFALVT
STNGRATVSEDNPANLGAFNMAPESQALYKTIDLMIVVGSKLRGNETLNNTMRLPRPTIQIDADASQGGRNYPVDLFVHG
DAADTLQRLLDRLPQKLSVDPNLVFDVARTRAQGEGTMRAAMGPYGSLADALLERVAPGRHPWVRDVTISNSTFGNRYVK
FAEPRHGVHALGGGIGQGIAMGIGAAIASPSAKAITLLGDGGAQLGIAEMITAVEERAPLVYVLMNDQAYGVIRNIQDAQ
YNSRHHYSALRTPNFAAHCASIGLPHRKVTAVADFADALDAALAADGPQLIEIDMLAIGPFAESFSGPPAGAAGNKV

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI21361361, Length=516, Percent_Identity=23.6434108527132, Blast_Score=91, Evalue=2e-18,
Organism=Escherichia coli, GI87081685, Length=511, Percent_Identity=25.8317025440313, Blast_Score=134, Evalue=2e-32,
Organism=Escherichia coli, GI1790104, Length=556, Percent_Identity=26.4388489208633, Blast_Score=128, Evalue=9e-31,
Organism=Escherichia coli, GI1786717, Length=480, Percent_Identity=23.75, Blast_Score=79, Evalue=8e-16,
Organism=Escherichia coli, GI1788716, Length=492, Percent_Identity=23.1707317073171, Blast_Score=66, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6323755, Length=572, Percent_Identity=22.7272727272727, Blast_Score=117, Evalue=4e-27,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 58758; Mature: 58758

Theoretical pI: Translated: 7.47; Mature: 7.47

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLESITRHETVGDFIAAYLADIGVEVIFGVISIHNMPILDAIARQGRVRFIPARGEAGA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCEEEEECCCCCCC
MNMADAYARVSGKLGVVMTSTGTAAGNAAGAQVEALTAGTSLLHITSTVDTQFMDRDRAP
CCHHHHHHHHCCCEEEEEEECCCCCCCCCCCEEEEEECCCEEEEEEHHHHHHHHCCCCCC
IHDVPRQPDMLKGISKAYYRLWDAKSAIGTLTAAVTAAISAPTGPVSLEIPVDVQRMAAL
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHCC
PTTRVFAPQPQSPRANDAAIEEIARLVAGARRPMLWLGGGARGARKQASELVERGFALVT
CCCEEECCCCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHCCEEEEE
STNGRATVSEDNPANLGAFNMAPESQALYKTIDLMIVVGSKLRGNETLNNTMRLPRPTIQ
ECCCCEEECCCCCCCCCEECCCCCHHHHHHHHEEEEEECCCCCCCCHHHCCCCCCCCEEE
IDADASQGGRNYPVDLFVHGDAADTLQRLLDRLPQKLSVDPNLVFDVARTRAQGEGTMRA
EECCCCCCCCCCCEEEEEECCHHHHHHHHHHHCCHHHCCCCCHHHHHHHHHCCCCCCEEH
AMGPYGSLADALLERVAPGRHPWVRDVTISNSTFGNRYVKFAEPRHGVHALGGGIGQGIA
HCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCEEEECCCCCCHHHHCCCHHHHHH
MGIGAAIASPSAKAITLLGDGGAQLGIAEMITAVEERAPLVYVLMNDQAYGVIRNIQDAQ
HHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCEEEEEECCCCHHHHHCCCCCC
YNSRHHYSALRTPNFAAHCASIGLPHRKVTAVADFADALDAALAADGPQLIEIDMLAIGP
CCCCHHHHHHCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECC
FAESFSGPPAGAAGNKV
CHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MKLESITRHETVGDFIAAYLADIGVEVIFGVISIHNMPILDAIARQGRVRFIPARGEAGA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCEEEEECCCCCCC
MNMADAYARVSGKLGVVMTSTGTAAGNAAGAQVEALTAGTSLLHITSTVDTQFMDRDRAP
CCHHHHHHHHCCCEEEEEEECCCCCCCCCCCEEEEEECCCEEEEEEHHHHHHHHCCCCCC
IHDVPRQPDMLKGISKAYYRLWDAKSAIGTLTAAVTAAISAPTGPVSLEIPVDVQRMAAL
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHCC
PTTRVFAPQPQSPRANDAAIEEIARLVAGARRPMLWLGGGARGARKQASELVERGFALVT
CCCEEECCCCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHCCEEEEE
STNGRATVSEDNPANLGAFNMAPESQALYKTIDLMIVVGSKLRGNETLNNTMRLPRPTIQ
ECCCCEEECCCCCCCCCEECCCCCHHHHHHHHEEEEEECCCCCCCCHHHCCCCCCCCEEE
IDADASQGGRNYPVDLFVHGDAADTLQRLLDRLPQKLSVDPNLVFDVARTRAQGEGTMRA
EECCCCCCCCCCCEEEEEECCHHHHHHHHHHHCCHHHCCCCCHHHHHHHHHCCCCCCEEH
AMGPYGSLADALLERVAPGRHPWVRDVTISNSTFGNRYVKFAEPRHGVHALGGGIGQGIA
HCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCEEEECCCCCCHHHHCCCHHHHHH
MGIGAAIASPSAKAITLLGDGGAQLGIAEMITAVEERAPLVYVLMNDQAYGVIRNIQDAQ
HHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCEEEEEECCCCHHHHHCCCCCC
YNSRHHYSALRTPNFAAHCASIGLPHRKVTAVADFADALDAALAADGPQLIEIDMLAIGP
CCCCHHHHHHCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECC
FAESFSGPPAGAAGNKV
CHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]