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The map label for this gene is purU [H]

Identifier: 110632813

GI number: 110632813

Start: 498680

End: 499540

Strand: Reverse

Name: purU [H]

Synonym: Meso_0452

Alternate gene names: 110632813

Gene position: 499540-498680 (Counterclockwise)

Preceding gene: 110632814

Following gene: 110632807

Centisome position: 11.32

GC content: 58.77

Gene sequence:

>861_bases
ATGACCGAAAAGACTTTCGATATAAATCTCTCCTGCGAGGATCGGCCGGGCATCGTTGCGGCTGTGACGACGGAACTTGC
AGCGCTCGGCGCGAATATCGCTGAATCGAACCAGTTCTGGGACCGTCAGACCAATCGCTTCTTTATGCGCATCTCCTTCA
CCGTGCCGGAGAATATCGACCGCGATGCAATCAGCCGCGCGCTGAACCCTGCCATCGCCCGCTTTAACATGGAGACCGCG
CTAGCCGACCGCTCGCGCCGGCCCAAGATCATCATCATGGTCTCGAAATTCGATCATGCTCTCCTCCATCTGCTTTACCA
GATCCGCGTCGGCTGGCTTGATTCGGAGGTGGCTGCGATCGTGTCCAACCATGAGGATTCGCGGCGCACCGCCGAGGCCG
CAGGCATTCCCTATTACTGCTGGCCGGTAACCAAGGCGAACAAGGCTGAGCAGGAAGAGAAGCTTTTGAACCTCTTTCGC
GAGACCGGTTCGGACCTGATCATCCTGGCGCGGTACATGCAGGTGCTATCGGACCAGCTGTCCAGCCGGCTTTTCGGCAA
GATCATCAACATCCATCACTCATTCCTGCCGAGCTTCAAGGGTGCGAAGCCTTACCATCAGGCGCACGAACGCGGCGTGA
AGCTAATCGGCGCCACAGCCCACTATGTGTCGCCGGATCTCGATGAAGGCCCGATCATCGAGCAGGAGACGGAACGCGTA
ACACACGCGATGAGCCCTGACGATTTCGTGGCCGCCGGTCGCGATATCGAGAGCCGTGTCCTGGCGAGGGCGGTCAAGCT
TCACCTCGAAAGCAGGGTAGTGCTCAACGGTCTGAAGACGGTGGTGTTCGCGAAGGGGTGA

Upstream 100 bases:

>100_bases
CTAACGTATCCAAGGCATCGGCATTGGCTTCCTGCGGTGCAGGTATTAAGAAGCAATGGTTCGCAAGCCCTCACCTGCGG
CTCTCGCCGTTGGAGTTTCC

Downstream 100 bases:

>100_bases
GCGGCTACTGCCCGATCTCTATCTCGCACCAGTGCCTATATGGCCGCTCCGGGGTCACGACGCAGTTGGGGAAATGCGGG
TGATTGATTGCATCGGGGAA

Product: formyltetrahydrofolate deformylase

Products: NA

Alternate protein names: Formyl-FH(4) hydrolase [H]

Number of amino acids: Translated: 286; Mature: 285

Protein sequence:

>286_residues
MTEKTFDINLSCEDRPGIVAAVTTELAALGANIAESNQFWDRQTNRFFMRISFTVPENIDRDAISRALNPAIARFNMETA
LADRSRRPKIIIMVSKFDHALLHLLYQIRVGWLDSEVAAIVSNHEDSRRTAEAAGIPYYCWPVTKANKAEQEEKLLNLFR
ETGSDLIILARYMQVLSDQLSSRLFGKIINIHHSFLPSFKGAKPYHQAHERGVKLIGATAHYVSPDLDEGPIIEQETERV
THAMSPDDFVAAGRDIESRVLARAVKLHLESRVVLNGLKTVVFAKG

Sequences:

>Translated_286_residues
MTEKTFDINLSCEDRPGIVAAVTTELAALGANIAESNQFWDRQTNRFFMRISFTVPENIDRDAISRALNPAIARFNMETA
LADRSRRPKIIIMVSKFDHALLHLLYQIRVGWLDSEVAAIVSNHEDSRRTAEAAGIPYYCWPVTKANKAEQEEKLLNLFR
ETGSDLIILARYMQVLSDQLSSRLFGKIINIHHSFLPSFKGAKPYHQAHERGVKLIGATAHYVSPDLDEGPIIEQETERV
THAMSPDDFVAAGRDIESRVLARAVKLHLESRVVLNGLKTVVFAKG
>Mature_285_residues
TEKTFDINLSCEDRPGIVAAVTTELAALGANIAESNQFWDRQTNRFFMRISFTVPENIDRDAISRALNPAIARFNMETAL
ADRSRRPKIIIMVSKFDHALLHLLYQIRVGWLDSEVAAIVSNHEDSRRTAEAAGIPYYCWPVTKANKAEQEEKLLNLFRE
TGSDLIILARYMQVLSDQLSSRLFGKIINIHHSFLPSFKGAKPYHQAHERGVKLIGATAHYVSPDLDEGPIIEQETERVT
HAMSPDDFVAAGRDIESRVLARAVKLHLESRVVLNGLKTVVFAKG

Specific function: Produces Formate From Formyl-Tetrahydrofolate. Provides The Major Source Of Formate For The Purt-Dependent Synthesis Of 5'-Phosphoribosyl-N-Formylglycinamide (Fgar) During Aerobic Growth. Has A Role In Regulating The One-Carbon Pool. [C]

COG id: COG0788

COG function: function code F; Formyltetrahydrofolate hydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=154, Percent_Identity=31.8181818181818, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI209869995, Length=154, Percent_Identity=31.8181818181818, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI209869993, Length=154, Percent_Identity=31.8181818181818, Blast_Score=79, Evalue=5e-15,
Organism=Escherichia coli, GI1787483, Length=279, Percent_Identity=39.7849462365591, Blast_Score=203, Evalue=1e-53,
Organism=Escherichia coli, GI1788846, Length=166, Percent_Identity=28.3132530120482, Blast_Score=70, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17567511, Length=152, Percent_Identity=33.5526315789474, Blast_Score=86, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR004810 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =3.5.1.10 [H]

Molecular weight: Translated: 32153; Mature: 32022

Theoretical pI: Translated: 7.37; Mature: 7.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEKTFDINLSCEDRPGIVAAVTTELAALGANIAESNQFWDRQTNRFFMRISFTVPENID
CCCCEEEEEEEECCCCCEEEHHHHHHHHHCCCHHHCCHHHHCCCCEEEEEEEEECCCCCC
RDAISRALNPAIARFNMETALADRSRRPKIIIMVSKFDHALLHLLYQIRVGWLDSEVAAI
HHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHHCCCCHHHHHH
VSNHEDSRRTAEAAGIPYYCWPVTKANKAEQEEKLLNLFRETGSDLIILARYMQVLSDQL
HHCCCHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHH
SSRLFGKIINIHHSFLPSFKGAKPYHQAHERGVKLIGATAHYVSPDLDEGPIIEQETERV
HHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCEEEECCHHHCCCCCCCCCCCCHHHHHH
THAMSPDDFVAAGRDIESRVLARAVKLHLESRVVLNGLKTVVFAKG
HHCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEECC
>Mature Secondary Structure 
TEKTFDINLSCEDRPGIVAAVTTELAALGANIAESNQFWDRQTNRFFMRISFTVPENID
CCCEEEEEEEECCCCCEEEHHHHHHHHHCCCHHHCCHHHHCCCCEEEEEEEEECCCCCC
RDAISRALNPAIARFNMETALADRSRRPKIIIMVSKFDHALLHLLYQIRVGWLDSEVAAI
HHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHHCCCCHHHHHH
VSNHEDSRRTAEAAGIPYYCWPVTKANKAEQEEKLLNLFRETGSDLIILARYMQVLSDQL
HHCCCHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHH
SSRLFGKIINIHHSFLPSFKGAKPYHQAHERGVKLIGATAHYVSPDLDEGPIIEQETERV
HHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCEEEECCHHHCCCCCCCCCCCCHHHHHH
THAMSPDDFVAAGRDIESRVLARAVKLHLESRVVLNGLKTVVFAKG
HHCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7543100 [H]