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The map label for this gene is gsiB [H]

Identifier: 110632436

GI number: 110632436

Start: 99970

End: 101514

Strand: Direct

Name: gsiB [H]

Synonym: Meso_0074

Alternate gene names: 110632436

Gene position: 99970-101514 (Clockwise)

Preceding gene: 110632435

Following gene: 110632437

Centisome position: 2.27

GC content: 60.91

Gene sequence:

>1545_bases
ATGTCGAGGCTTCACGCTCTCACGATGTCCCGCCGTGCTTTCCTCGCCGCCGCAGGCGCCGGAATCGCAGCCGTCCAGTT
GCCCGGTTCGGCTTCCGCCCAGGCCGATGCCGGTGTTTTGCGCTACGCTCTCTCCGCCTTTCCGCCAAGCCTTAGCCCTT
GGCTCAACGCCGGGGCAGCGGCATCCACGGTTAAGACGCTGATCATGCGCGGCCTGCTGGGTTACGATACCGAAGGACAG
CTGATCGGCGAGATCGCTGAAAGCTGGGCTGCGGTGGATCCGACCACCTATGATTTCAAGCTCCGCGCCAATGCCGTTTT
CAGCAATGGCAAACCCGTGCGGGCTCAGGACGTCATCTGGACCGTCAATCAGATCAGCGCTCCCGAATCGACCGCTCACC
TCAGGACAACGCTCAACCAGGTGGAGTCCATGGAGGCGCTTGACGACCACACCGTGCGGTTGATCTTGACCAAGCCCAAT
GCGGCATTGCCATTTGCCCTCGCCAGCCCGAACGCTGCCATTCTTGCCGAGGGCAGCACAGAGGATGCACCCATTGCCTG
CGGCCCCTTCCGTCTGGCCCAGCAGGAGCGTGGTGTCTTCATCGAGGTCGAGCGCAATCCCGACTACTATAAGAACGGTT
CGCCCCAGGTCGACAAAATCCGGCTCACGGCCTATGCCGATGAAAACCTTCGCATGGCTGCGCTGGAATCGGGCGATGTC
GACATCATCGAGTATGTGCCCTGGTCTGCCTATTCCCGCTTCGACAATAACGACCGCATGAAACTGTCGGGTTCGCTCGG
CCCCTTCCATGTGGTGCTTTTCAACACGAGCAAGCCGCCCTTCGACAATCCGCTGGTGCGCCAGGCGATCGGCTATGCCA
TCAACCGCGAGGACATCGTGGCAGCAGCCTTCGCCGGCCAAGGCGAACCGCTTTATGGCTTCCCCAATCCTCCTGGTTCG
GCCTACCAGGCCTCCACGCCGGAAAACAGCTATACTTACGATCCGGAAAGGGCCAAAGCGCTGCTGGCCGAGGCTGGCTA
TGCCGATGGCTTCAACTGTACGCTTCTGGCAACCTCAACCTATGGCATGCTCCAGGACACGGCCGTGGTCGTTGCTGCCT
ATCTCCAGGCTATCGGCATCAATGCCGAACTCAAGCTCCCCGAATGGGCCAGTCGCATCACCGCCGGCAATGAAGGCCAG
TATGACATTGCCATCCATGGCACGGTGGGCGAGTACAACGACCCGGACTCGCTCTACCCGCTGCTCCACTCCTCGGTGAA
GACGTTCCAGAACTCTTTCGGCTTCGCCAGCGAGCGTATCGATACCCTGCTCGAAAAGGGACGAGGCGAACTGGATCCGG
CCAAGCGCGAGGAGATCTACAAACAGCTGGCCCAGGCCTATTTCGAGGAGGTGCCGCAAGCGCCCCTGGTCTGGCGTGTT
CAATCCTTCGGCCTTGCCCGCAATGTGGAAGGATTCCAATGCCTGCCAGGTTTCCTCGGGGTGACGAGCACCGCGATCCT
GCTCGATCAGGCCAGGTTCGTCTGA

Upstream 100 bases:

>100_bases
CCGCCTAAAGAATTTTAAATTTGCCGGTGGGCTGATCGCGTCCGAAAATCTCCGACGAACATGCAAGGCAAAAAAGCCAG
CAAATTGGAGGAGATAACTT

Downstream 100 bases:

>100_bases
CAAATGTTGCACTCTGCGATCTCCCGGCTGGCGTCGGCCCTGAGCATGCTCTTTGTGGTCGCGACATTGGTCTTCGCCAT
CCAAGTCGTCATCCCCGGCG

Product: twin-arginine translocation pathway signal

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 514; Mature: 513

Protein sequence:

>514_residues
MSRLHALTMSRRAFLAAAGAGIAAVQLPGSASAQADAGVLRYALSAFPPSLSPWLNAGAAASTVKTLIMRGLLGYDTEGQ
LIGEIAESWAAVDPTTYDFKLRANAVFSNGKPVRAQDVIWTVNQISAPESTAHLRTTLNQVESMEALDDHTVRLILTKPN
AALPFALASPNAAILAEGSTEDAPIACGPFRLAQQERGVFIEVERNPDYYKNGSPQVDKIRLTAYADENLRMAALESGDV
DIIEYVPWSAYSRFDNNDRMKLSGSLGPFHVVLFNTSKPPFDNPLVRQAIGYAINREDIVAAAFAGQGEPLYGFPNPPGS
AYQASTPENSYTYDPERAKALLAEAGYADGFNCTLLATSTYGMLQDTAVVVAAYLQAIGINAELKLPEWASRITAGNEGQ
YDIAIHGTVGEYNDPDSLYPLLHSSVKTFQNSFGFASERIDTLLEKGRGELDPAKREEIYKQLAQAYFEEVPQAPLVWRV
QSFGLARNVEGFQCLPGFLGVTSTAILLDQARFV

Sequences:

>Translated_514_residues
MSRLHALTMSRRAFLAAAGAGIAAVQLPGSASAQADAGVLRYALSAFPPSLSPWLNAGAAASTVKTLIMRGLLGYDTEGQ
LIGEIAESWAAVDPTTYDFKLRANAVFSNGKPVRAQDVIWTVNQISAPESTAHLRTTLNQVESMEALDDHTVRLILTKPN
AALPFALASPNAAILAEGSTEDAPIACGPFRLAQQERGVFIEVERNPDYYKNGSPQVDKIRLTAYADENLRMAALESGDV
DIIEYVPWSAYSRFDNNDRMKLSGSLGPFHVVLFNTSKPPFDNPLVRQAIGYAINREDIVAAAFAGQGEPLYGFPNPPGS
AYQASTPENSYTYDPERAKALLAEAGYADGFNCTLLATSTYGMLQDTAVVVAAYLQAIGINAELKLPEWASRITAGNEGQ
YDIAIHGTVGEYNDPDSLYPLLHSSVKTFQNSFGFASERIDTLLEKGRGELDPAKREEIYKQLAQAYFEEVPQAPLVWRV
QSFGLARNVEGFQCLPGFLGVTSTAILLDQARFV
>Mature_513_residues
SRLHALTMSRRAFLAAAGAGIAAVQLPGSASAQADAGVLRYALSAFPPSLSPWLNAGAAASTVKTLIMRGLLGYDTEGQL
IGEIAESWAAVDPTTYDFKLRANAVFSNGKPVRAQDVIWTVNQISAPESTAHLRTTLNQVESMEALDDHTVRLILTKPNA
ALPFALASPNAAILAEGSTEDAPIACGPFRLAQQERGVFIEVERNPDYYKNGSPQVDKIRLTAYADENLRMAALESGDVD
IIEYVPWSAYSRFDNNDRMKLSGSLGPFHVVLFNTSKPPFDNPLVRQAIGYAINREDIVAAAFAGQGEPLYGFPNPPGSA
YQASTPENSYTYDPERAKALLAEAGYADGFNCTLLATSTYGMLQDTAVVVAAYLQAIGINAELKLPEWASRITAGNEGQY
DIAIHGTVGEYNDPDSLYPLLHSSVKTFQNSFGFASERIDTLLEKGRGELDPAKREEIYKQLAQAYFEEVPQAPLVWRVQ
SFGLARNVEGFQCLPGFLGVTSTAILLDQARFV

Specific function: Part of the ABC transporter complex gsiABCD involved in glutathione import [H]

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787052, Length=493, Percent_Identity=28.1947261663286, Blast_Score=178, Evalue=8e-46,
Organism=Escherichia coli, GI1789966, Length=491, Percent_Identity=26.4765784114053, Blast_Score=147, Evalue=2e-36,
Organism=Escherichia coli, GI1787495, Length=527, Percent_Identity=24.8576850094877, Blast_Score=132, Evalue=5e-32,
Organism=Escherichia coli, GI87081878, Length=516, Percent_Identity=23.2558139534884, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1787551, Length=532, Percent_Identity=24.4360902255639, Blast_Score=118, Evalue=9e-28,
Organism=Escherichia coli, GI1789887, Length=452, Percent_Identity=25, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1787762, Length=496, Percent_Identity=24.5967741935484, Blast_Score=105, Evalue=1e-23,
Organism=Escherichia coli, GI1789397, Length=488, Percent_Identity=23.155737704918, Blast_Score=96, Evalue=8e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 55632; Mature: 55501

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRLHALTMSRRAFLAAAGAGIAAVQLPGSASAQADAGVLRYALSAFPPSLSPWLNAGAA
CCCHHHHHHHHHHHEEECCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCHHHCCCHH
ASTVKTLIMRGLLGYDTEGQLIGEIAESWAAVDPTTYDFKLRANAVFSNGKPVRAQDVIW
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCEEEEEEEEEEEECCCCCCCHHHEEE
TVNQISAPESTAHLRTTLNQVESMEALDDHTVRLILTKPNAALPFALASPNAAILAEGST
EEHHCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCEEECCCCEEEEECCCC
EDAPIACGPFRLAQQERGVFIEVERNPDYYKNGSPQVDKIRLTAYADENLRMAALESGDV
CCCCEEECCHHHHHCCCCEEEEEECCCCCCCCCCCCCEEEEEEEEECCCEEEEEECCCCE
DIIEYVPWSAYSRFDNNDRMKLSGSLGPFHVVLFNTSKPPFDNPLVRQAIGYAINREDIV
EEEEECCCHHHHHCCCCCCEEEECCCCCEEEEEECCCCCCCCCHHHHHHHHCCCCCCCEE
AAAFAGQGEPLYGFPNPPGSAYQASTPENSYTYDPERAKALLAEAGYADGFNCTLLATST
EEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEEECC
YGMLQDTAVVVAAYLQAIGINAELKLPEWASRITAGNEGQYDIAIHGTVGEYNDPDSLYP
CCHHHHHHHHHHHHHHHHCCCCEEECHHHHHHHCCCCCCCEEEEEEECCCCCCCCHHHHH
LLHSSVKTFQNSFGFASERIDTLLEKGRGELDPAKREEIYKQLAQAYFEEVPQAPLVWRV
HHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEE
QSFGLARNVEGFQCLPGFLGVTSTAILLDQARFV
HHCCCCCCCCCCHHCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SRLHALTMSRRAFLAAAGAGIAAVQLPGSASAQADAGVLRYALSAFPPSLSPWLNAGAA
CCHHHHHHHHHHHEEECCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCHHHCCCHH
ASTVKTLIMRGLLGYDTEGQLIGEIAESWAAVDPTTYDFKLRANAVFSNGKPVRAQDVIW
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCEEEEEEEEEEEECCCCCCCHHHEEE
TVNQISAPESTAHLRTTLNQVESMEALDDHTVRLILTKPNAALPFALASPNAAILAEGST
EEHHCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCEEECCCCEEEEECCCC
EDAPIACGPFRLAQQERGVFIEVERNPDYYKNGSPQVDKIRLTAYADENLRMAALESGDV
CCCCEEECCHHHHHCCCCEEEEEECCCCCCCCCCCCCEEEEEEEEECCCEEEEEECCCCE
DIIEYVPWSAYSRFDNNDRMKLSGSLGPFHVVLFNTSKPPFDNPLVRQAIGYAINREDIV
EEEEECCCHHHHHCCCCCCEEEECCCCCEEEEEECCCCCCCCCHHHHHHHHCCCCCCCEE
AAAFAGQGEPLYGFPNPPGSAYQASTPENSYTYDPERAKALLAEAGYADGFNCTLLATST
EEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEEECC
YGMLQDTAVVVAAYLQAIGINAELKLPEWASRITAGNEGQYDIAIHGTVGEYNDPDSLYP
CCHHHHHHHHHHHHHHHHCCCCEEECHHHHHHHCCCCCCCEEEEEEECCCCCCCCHHHHH
LLHSSVKTFQNSFGFASERIDTLLEKGRGELDPAKREEIYKQLAQAYFEEVPQAPLVWRV
HHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEE
QSFGLARNVEGFQCLPGFLGVTSTAILLDQARFV
HHCCCCCCCCCCHHCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA