Click here to switch to the map view.

The map label for this gene is recF [H]

Identifier: 110632365

GI number: 110632365

Start: 2827

End: 4002

Strand: Direct

Name: recF [H]

Synonym: Meso_0003

Alternate gene names: 110632365

Gene position: 2827-4002 (Clockwise)

Preceding gene: 110632364

Following gene: 110632366

Centisome position: 0.06

GC content: 64.37

Gene sequence:

>1176_bases
GTGACGACGACGGCCACGACAGGAACTCCGGAAAAGCGGCCGGCGCAGGTCCATATCGCCAAGCTGACGCTCTCCAATTT
TCGCAACTACGCCTCGCTCTCGCTCGGCCTGCAGCCTGGCGCGGTGGTGCTTACCGGCGAGAACGGCGCCGGCAAGACGA
ACCTGCTCGAAGCAATCTCCTTTCTCTCCCCCGGTCGTGGACTGCGGCGCGCCACATTGGAAGAGGCGATGCGCATCGGC
TCCAGCGATGGTTTTGCGGTGCATGCGGAGGTGGAAGGACCTTATGGCTCCTGTCGGATTGGGACGGGAACGGCCGGTAC
GGCAGCTGAGGGATCAGAATCCGGGCGACGTGTGCGCATCGATGGCGAGCCCCAGCGCAGCGCTGAAGCCATGCTCGATT
GGTTGCGGGTGATCTGGCTTACCCCGGCTATGGACGCGCTCTTCACCGGGGCTTCGGCTGATCGCAGGCGCTTTTTGGAC
CGCCTTGTCCTGGCAATCGATCCAGCGCATGGCCGCCGGGCGGCGGATTATGAAAAAGCCATGCGCTCCCGCAACCGGCT
TTTCGCGGATGATGTGCGGGACGATGCCTGGTTCGATGCCATCGAGATGCAGATGGCCGAAACCGGCGTGGCGATTGCCG
CGGCGCGTGCCGAGATGTTGCGGCTGCTTGCCGCAATGATTGAGCGTCTTCCTGCCGGCAGTCCCTTTCCCAAGGCGCTG
CTCGCGCTAGAAGGCACGGTGGACGAGGCGATTGCACGAAACCCGGCTGTCGAGGTTGAGGAGGATTTCCGTACTCGCTT
GCGCGAAGAGCGCCCGAGAGACCGCGCGGCGGGGCGGGCGCTCGAAGGGCCGCACCGCAGCGAGCTCCTGGTGCGCCATG
CGCCCAAGGACATGCCGGCGGAAAGCTGTTCGACCGGAGAACAAAAGGCGCTTCTCGTTGGGCTCGTGCTTGCCCATGCA
CGGCTGACCGCTGAGCTTTCAGGCATGGCGCCGATTCTGCTCCTGGACGAAATCTCCGCCCATTTCGATGCCGATCGCCG
CGCGGCCCTCTTCGACATCCTCGAGGATCTCAACTGCCAGGCCTTCATGACCGGTACGGAACGCGCGCTCTTCTCCAGCC
TGGAGGGCCGGGCCCAATTTCTCGCCGTATCGGGCGGCGCAATTCAGTCGGTTTGA

Upstream 100 bases:

>100_bases
GCAGTGAAGCGAAGTTTCTCCTGGCCGACGCCGGCTCGCCGACGCTCATCCAGGATACGTCCGACGAGCGCGTGCTTTAC
GTCCTGATGCCGATGCGGGT

Downstream 100 bases:

>100_bases
GCGCGGCGCACCTGACAAGCCGCCTCCGGCGAAGTATGGTCCCGCCATGAACGATACGATCCTTTCCGACGAAGAGCTTG
AGCGCTACGCGCGCCATATC

Product: recombination protein F

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 391; Mature: 390

Protein sequence:

>391_residues
MTTTATTGTPEKRPAQVHIAKLTLSNFRNYASLSLGLQPGAVVLTGENGAGKTNLLEAISFLSPGRGLRRATLEEAMRIG
SSDGFAVHAEVEGPYGSCRIGTGTAGTAAEGSESGRRVRIDGEPQRSAEAMLDWLRVIWLTPAMDALFTGASADRRRFLD
RLVLAIDPAHGRRAADYEKAMRSRNRLFADDVRDDAWFDAIEMQMAETGVAIAAARAEMLRLLAAMIERLPAGSPFPKAL
LALEGTVDEAIARNPAVEVEEDFRTRLREERPRDRAAGRALEGPHRSELLVRHAPKDMPAESCSTGEQKALLVGLVLAHA
RLTAELSGMAPILLLDEISAHFDADRRAALFDILEDLNCQAFMTGTERALFSSLEGRAQFLAVSGGAIQSV

Sequences:

>Translated_391_residues
MTTTATTGTPEKRPAQVHIAKLTLSNFRNYASLSLGLQPGAVVLTGENGAGKTNLLEAISFLSPGRGLRRATLEEAMRIG
SSDGFAVHAEVEGPYGSCRIGTGTAGTAAEGSESGRRVRIDGEPQRSAEAMLDWLRVIWLTPAMDALFTGASADRRRFLD
RLVLAIDPAHGRRAADYEKAMRSRNRLFADDVRDDAWFDAIEMQMAETGVAIAAARAEMLRLLAAMIERLPAGSPFPKAL
LALEGTVDEAIARNPAVEVEEDFRTRLREERPRDRAAGRALEGPHRSELLVRHAPKDMPAESCSTGEQKALLVGLVLAHA
RLTAELSGMAPILLLDEISAHFDADRRAALFDILEDLNCQAFMTGTERALFSSLEGRAQFLAVSGGAIQSV
>Mature_390_residues
TTTATTGTPEKRPAQVHIAKLTLSNFRNYASLSLGLQPGAVVLTGENGAGKTNLLEAISFLSPGRGLRRATLEEAMRIGS
SDGFAVHAEVEGPYGSCRIGTGTAGTAAEGSESGRRVRIDGEPQRSAEAMLDWLRVIWLTPAMDALFTGASADRRRFLDR
LVLAIDPAHGRRAADYEKAMRSRNRLFADDVRDDAWFDAIEMQMAETGVAIAAARAEMLRLLAAMIERLPAGSPFPKALL
ALEGTVDEAIARNPAVEVEEDFRTRLREERPRDRAAGRALEGPHRSELLVRHAPKDMPAESCSTGEQKALLVGLVLAHAR
LTAELSGMAPILLLDEISAHFDADRRAALFDILEDLNCQAFMTGTERALFSSLEGRAQFLAVSGGAIQSV

Specific function: The recF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP [H]

COG id: COG1195

COG function: function code L; Recombinational DNA repair ATPase (RecF pathway)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recF family [H]

Homologues:

Organism=Escherichia coli, GI1790135, Length=350, Percent_Identity=26, Blast_Score=92, Evalue=8e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR001238
- InterPro:   IPR018078
- InterPro:   IPR003395
- InterPro:   IPR007406 [H]

Pfam domain/function: PF02463 SMC_N [H]

EC number: NA

Molecular weight: Translated: 42217; Mature: 42085

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: PS00617 RECF_1 ; PS00687 ALDEHYDE_DEHYDR_GLU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTTATTGTPEKRPAQVHIAKLTLSNFRNYASLSLGLQPGAVVLTGENGAGKTNLLEAIS
CCCCCCCCCCCCCCCEEEEEEEEHHHCCCCEEEEECCCCCEEEEECCCCCCHHHHHHHHH
FLSPGRGLRRATLEEAMRIGSSDGFAVHAEVEGPYGSCRIGTGTAGTAAEGSESGRRVRI
HHCCCCCHHHHHHHHHHHCCCCCCEEEEEEECCCCCCEEECCCCCCCCCCCCCCCCEEEE
DGEPQRSAEAMLDWLRVIWLTPAMDALFTGASADRRRFLDRLVLAIDPAHGRRAADYEKA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHH
MRSRNRLFADDVRDDAWFDAIEMQMAETGVAIAAARAEMLRLLAAMIERLPAGSPFPKAL
HHHCCCEEHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
LALEGTVDEAIARNPAVEVEEDFRTRLREERPRDRAAGRALEGPHRSELLVRHAPKDMPA
HHHCCCHHHHHHCCCCCCCHHHHHHHHHHHCCHHHHCCCCCCCCCHHHHHHHCCCCCCCC
ESCSTGEQKALLVGLVLAHARLTAELSGMAPILLLDEISAHFDADRRAALFDILEDLNCQ
HHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHCCCCHHHHHHHHHHHCCCE
AFMTGTERALFSSLEGRAQFLAVSGGAIQSV
EEECCHHHHHHHHHCCCEEEEEECCCHHCCC
>Mature Secondary Structure 
TTTATTGTPEKRPAQVHIAKLTLSNFRNYASLSLGLQPGAVVLTGENGAGKTNLLEAIS
CCCCCCCCCCCCCCEEEEEEEEHHHCCCCEEEEECCCCCEEEEECCCCCCHHHHHHHHH
FLSPGRGLRRATLEEAMRIGSSDGFAVHAEVEGPYGSCRIGTGTAGTAAEGSESGRRVRI
HHCCCCCHHHHHHHHHHHCCCCCCEEEEEEECCCCCCEEECCCCCCCCCCCCCCCCEEEE
DGEPQRSAEAMLDWLRVIWLTPAMDALFTGASADRRRFLDRLVLAIDPAHGRRAADYEKA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHH
MRSRNRLFADDVRDDAWFDAIEMQMAETGVAIAAARAEMLRLLAAMIERLPAGSPFPKAL
HHHCCCEEHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
LALEGTVDEAIARNPAVEVEEDFRTRLREERPRDRAAGRALEGPHRSELLVRHAPKDMPA
HHHCCCHHHHHHCCCCCCCHHHHHHHHHHHCCHHHHCCCCCCCCCHHHHHHHCCCCCCCC
ESCSTGEQKALLVGLVLAHARLTAELSGMAPILLLDEISAHFDADRRAALFDILEDLNCQ
HHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHCCCCHHHHHHHHHHHCCCE
AFMTGTERALFSSLEGRAQFLAVSGGAIQSV
EEECCHHHHHHHHHCCCEEEEEECCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11214968 [H]