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The map label for this gene is dnaN [H]

Identifier: 110632364

GI number: 110632364

Start: 1712

End: 2830

Strand: Direct

Name: dnaN [H]

Synonym: Meso_0002

Alternate gene names: 110632364

Gene position: 1712-2830 (Clockwise)

Preceding gene: 110632363

Following gene: 110632365

Centisome position: 0.04

GC content: 59.52

Gene sequence:

>1119_bases
ATGCGCGTAGTCCTTGAACGTTCCAATCTTCTGAAGTCGCTCGGCCATGTTCATCGGGTGGTGGAACGGCGTAATACCAT
TCCCATTCTCTCCAATGTGCTGCTTTCAGCTGATGGTGCGAGCCTGGAGATGAAAGCGACCGACCTCGATCTGGAGATGA
CGGAAGCAGCGCCGGCCTCCGTGGAGCAGGGAGGCGCCACCACCGTGCCTGCGCATCTGCTCTACGATATCGTCCGCAAG
CTGCCGGACGGTGCTGAGGTGATGTTGAAGGTGGATGAGAACGGCCAGACCATGTCGGTCATTTCGGGCCGTTCCACCTT
CCGCTTGCAATGCCTGCCCAAGGCGGATTTCCCGGCCCTTACGGCTGGCCAGTTTTCGCATATTTTCCGCATGGAATCCG
CCGTCCTGCGCAGCCTGGTGGAGAAGACCCAGTTCGCCATCTCGACCGAGGAGACGCGTTATTATTTGAACGGCATCTTC
CTTCACACGCTTGAATCGGATGGAGCGCTCAAGCTTCGGGCCGTGGCAACCGATGGCCACCGCCTTGCGCGGGCGGAGAT
CGAGGCGCCCGCCGGTTCGGAAGGGATGCCCGGCATCATTGTACCGCGCAAAACCGTTGGCGAATTGCAGAAGCTGGTGG
ACGATCCGGATGTTGCCGTCACTGTGGAGCTTTCGGACACGAAAATCCGCTTCACAATTGGCAGTGTTGTGCTTACGTCC
AAGCTGATCGACGGCACGTTCCCCGATTATCAGCGCGTCATCCCGGTAGGCAACGACAAGGAACTGCTCATCGACAGGCA
GAGCTTCTCGGCGGCGGTCGATCGTGTCTCCACCGTCTCCTCGGAGCGCGGACGGGCGGTGAAACTTTCCATTTCGGACG
GTCAGGTGACCTTTACGGTCAACAATCCGGATTCGGGCAGCGCGACGGAAGAAATACCTGCGGGCTATAACGCGGATCCC
ATCGAAATCGGTTTCAACGCGCGCTACCTCTTGGACGTTGCAGCGCAACTGTCGGGCAGTGAAGCGAAGTTTCTCCTGGC
CGACGCCGGCTCGCCGACGCTCATCCAGGATACGTCCGACGAGCGCGTGCTTTACGTCCTGATGCCGATGCGGGTGTGA

Upstream 100 bases:

>100_bases
TTTTCCGGTTTTCCTGTCAGTTTGGCGCAATTTCTATCAGGTCGAGGCGGGCCGTGACGGCATTCGCCAGGCCGCTCCTC
ATCAGGCGAGAACAGAGGTT

Downstream 100 bases:

>100_bases
CGACGACGGCCACGACAGGAACTCCGGAAAAGCGGCCGGCGCAGGTCCATATCGCCAAGCTGACGCTCTCCAATTTTCGC
AACTACGCCTCGCTCTCGCT

Product: DNA polymerase III subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 372; Mature: 372

Protein sequence:

>372_residues
MRVVLERSNLLKSLGHVHRVVERRNTIPILSNVLLSADGASLEMKATDLDLEMTEAAPASVEQGGATTVPAHLLYDIVRK
LPDGAEVMLKVDENGQTMSVISGRSTFRLQCLPKADFPALTAGQFSHIFRMESAVLRSLVEKTQFAISTEETRYYLNGIF
LHTLESDGALKLRAVATDGHRLARAEIEAPAGSEGMPGIIVPRKTVGELQKLVDDPDVAVTVELSDTKIRFTIGSVVLTS
KLIDGTFPDYQRVIPVGNDKELLIDRQSFSAAVDRVSTVSSERGRAVKLSISDGQVTFTVNNPDSGSATEEIPAGYNADP
IEIGFNARYLLDVAAQLSGSEAKFLLADAGSPTLIQDTSDERVLYVLMPMRV

Sequences:

>Translated_372_residues
MRVVLERSNLLKSLGHVHRVVERRNTIPILSNVLLSADGASLEMKATDLDLEMTEAAPASVEQGGATTVPAHLLYDIVRK
LPDGAEVMLKVDENGQTMSVISGRSTFRLQCLPKADFPALTAGQFSHIFRMESAVLRSLVEKTQFAISTEETRYYLNGIF
LHTLESDGALKLRAVATDGHRLARAEIEAPAGSEGMPGIIVPRKTVGELQKLVDDPDVAVTVELSDTKIRFTIGSVVLTS
KLIDGTFPDYQRVIPVGNDKELLIDRQSFSAAVDRVSTVSSERGRAVKLSISDGQVTFTVNNPDSGSATEEIPAGYNADP
IEIGFNARYLLDVAAQLSGSEAKFLLADAGSPTLIQDTSDERVLYVLMPMRV
>Mature_372_residues
MRVVLERSNLLKSLGHVHRVVERRNTIPILSNVLLSADGASLEMKATDLDLEMTEAAPASVEQGGATTVPAHLLYDIVRK
LPDGAEVMLKVDENGQTMSVISGRSTFRLQCLPKADFPALTAGQFSHIFRMESAVLRSLVEKTQFAISTEETRYYLNGIF
LHTLESDGALKLRAVATDGHRLARAEIEAPAGSEGMPGIIVPRKTVGELQKLVDDPDVAVTVELSDTKIRFTIGSVVLTS
KLIDGTFPDYQRVIPVGNDKELLIDRQSFSAAVDRVSTVSSERGRAVKLSISDGQVTFTVNNPDSGSATEEIPAGYNADP
IEIGFNARYLLDVAAQLSGSEAKFLLADAGSPTLIQDTSDERVLYVLMPMRV

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=375, Percent_Identity=40.2666666666667, Blast_Score=265, Evalue=4e-72,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 40325; Mature: 40325

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVVLERSNLLKSLGHVHRVVERRNTIPILSNVLLSADGASLEMKATDLDLEMTEAAPAS
CEEEEEHHHHHHHHHHHHHHHHHCCCCHHHHHHHEECCCCEEEEEEECCCEEECCCCCCC
VEQGGATTVPAHLLYDIVRKLPDGAEVMLKVDENGQTMSVISGRSTFRLQCLPKADFPAL
CCCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEEEECCCEEEEEEECCCCCCCC
TAGQFSHIFRMESAVLRSLVEKTQFAISTEETRYYLNGIFLHTLESDGALKLRAVATDGH
CCHHHHHHHHHHHHHHHHHHHHHHEEEECCCCEEEEEEEEEEEECCCCCEEEEEEECCCC
RLARAEIEAPAGSEGMPGIIVPRKTVGELQKLVDDPDVAVTVELSDTKIRFTIGSVVLTS
EEEEEEECCCCCCCCCCEEEECCHHHHHHHHHHCCCCEEEEEEECCCEEEEEEHHHHHHH
KLIDGTFPDYQRVIPVGNDKELLIDRQSFSAAVDRVSTVSSERGRAVKLSISDGQVTFTV
HHHCCCCCCCCEEEECCCCCEEEEECHHHHHHHHHHHHHHCCCCCEEEEEECCCEEEEEE
NNPDSGSATEEIPAGYNADPIEIGFNARYLLDVAAQLSGSEAKFLLADAGSPTLIQDTSD
CCCCCCCCCHHCCCCCCCCCEEECCCCEEEEEHHHHCCCCCCEEEEEECCCCCEEECCCC
ERVLYVLMPMRV
CEEEEEEEECCC
>Mature Secondary Structure
MRVVLERSNLLKSLGHVHRVVERRNTIPILSNVLLSADGASLEMKATDLDLEMTEAAPAS
CEEEEEHHHHHHHHHHHHHHHHHCCCCHHHHHHHEECCCCEEEEEEECCCEEECCCCCCC
VEQGGATTVPAHLLYDIVRKLPDGAEVMLKVDENGQTMSVISGRSTFRLQCLPKADFPAL
CCCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEEEECCCEEEEEEECCCCCCCC
TAGQFSHIFRMESAVLRSLVEKTQFAISTEETRYYLNGIFLHTLESDGALKLRAVATDGH
CCHHHHHHHHHHHHHHHHHHHHHHEEEECCCCEEEEEEEEEEEECCCCCEEEEEEECCCC
RLARAEIEAPAGSEGMPGIIVPRKTVGELQKLVDDPDVAVTVELSDTKIRFTIGSVVLTS
EEEEEEECCCCCCCCCCEEEECCHHHHHHHHHHCCCCEEEEEEECCCEEEEEEHHHHHHH
KLIDGTFPDYQRVIPVGNDKELLIDRQSFSAAVDRVSTVSSERGRAVKLSISDGQVTFTV
HHHCCCCCCCCEEEECCCCCEEEEECHHHHHHHHHHHHHHCCCCCEEEEEECCCEEEEEE
NNPDSGSATEEIPAGYNADPIEIGFNARYLLDVAAQLSGSEAKFLLADAGSPTLIQDTSD
CCCCCCCCCHHCCCCCCCCCEEECCCCEEEEEHHHHCCCCCCEEEEEECCCCCEEECCCC
ERVLYVLMPMRV
CEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9079919; 8226640 [H]