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The map label for this gene is dnaA [H]

Identifier: 110632363

GI number: 110632363

Start: 85

End: 1524

Strand: Direct

Name: dnaA [H]

Synonym: Meso_0001

Alternate gene names: 110632363

Gene position: 85-1524 (Clockwise)

Preceding gene: NA

Following gene: 110632364

Centisome position: 0.0

GC content: 60.83

Gene sequence:

>1440_bases
ATGGCAGTGTTCGAACGGGTTCGGGCACAACTGAAGGCCCGGCTCGGTACGGAGGTTTATTCCAGCTGGTTTGGGCGCAT
GAAGCTGGCGGAGACCTCCAAAGGTGTAGTTCGCATTTCTGTCCCGACGGCATTTCTGAGGGCCTGGATCAACGGCCACT
ATCTTGATCTGATCACCAGTCTTTGGAAGGCGGAGGATCCGTCCACCTTGAAGGTGGAGATTGTGGTTCGCACGGCGACG
CGCACATCGCCCGTCAAGGCGGATGTCAAGCCCGTGCCGGCGCGGAAGGCAAGTGGCCAGCCTCAGCAGGCGCTCGCGTC
GGTCGGGGTCAACGGGGCCCGGGTCACGCCCCTGCCTACGGCAAAAGCAGACCAGGCGATGCGGCCCAGCATGTTCGGAT
CGCCGCTCGACAGCCGCTTCACCTTTGAATCCTTCATTGAGGGCGCCTCGAACCGTGTTGCCTATGCGGCCGCCCGAACG
GTGGCGGAACTGGCGGCAGGGGCCATAAGGTTCAATCCGCTCTTCATTCATGCCTCGGTCGGCCTCGGGAAAACACACCT
CTTGCAGGCCGTCGCCGCGGCTTCCTTGAAGCGGCGCCCGCAATCGCGCGTTGTCTATCTTACCGCGGAATACTTCATGT
GGCGCTTTGCCACTGCGATACGCGACAACAACGCGCTTACCCTAAAGGAACAACTCCGGGACATCGATCTCCTCATTATC
GATGACATGCAGTTTCTGCAGGGCAAATCGATACAGAACGAGTTCTGCCACCTTCTCAATATGTTGCTCGACAGCGCCAG
GCAGGTGGTCGTGGCAGCCGACCGGCCGGTATCGGAATTGGAGTCGCTGGAGCCGCGCGTAAAGTCGCGTCTGAACGGCG
GCGTGTCTCTCGAGATGCTCACGCCGGATTACGACCTCAGGCTGGCCATGCTTAAGCAGCGTCTCGCCTGCGCTCGCGTC
GAGGATCCTTCCCTAGATATCCCTCTGGAAGTGCTGGAGCATGTGGCACAGGTGGTGACCGGCAGCGGGCGCGAGCTTGA
GGGTGCGTTCAACCAGCTCGTTTTCCGCCATTCCTTCGAACCGCAGATCACACTTGAGCGCATCGACGAAATCCTGGGTC
CCGTCTGTCGCACCGGCGAGCCGAGGCGCGTGCGCATCGAGGATATCCAGCGCATCGTCGCTCGGCATTACAATGTGTCG
AAGACGGAGCTTCTTTCCAATCGGCGCACCCGTTCCATCGTGAAGCCGCGCCAGGTGGCAATGTACCTTGCCAAGGTAAT
GACGCCTCGCTCGCTGCCGGAGATCGGCCGCCGCTTCGGCGGTCGCGATCACACGACGGTGCTGCATGCGGTGCGCAAAA
TCGAAGGCATGTCGGGGGACGACAAGCAGCTTGCGCAGGAGATCGAGCTTCTGAAGAGGCTGATCAGCGATCAAGCTTAA

Upstream 100 bases:

>100_bases
GGAAACGAGGATGCAAAGCGGCGTTGAGAGGGAAGTCGGGGGAGGGTTCTCTTTTCACGGCTCGGCGGCTCATGAAGAAG
CGGAGAGCTCCGGAGAGGCC

Downstream 100 bases:

>100_bases
GCGGGAGTGCGCCTAAAAACGCTCGTAGAAGCGTTATCCCCAGAAAGCCCGCGGAAATCGGCTAACGCCTTTTCGCGGGC
TTGCCTTTTTTCCGGTTTTC

Product: chromosomal replication initiation protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 479; Mature: 478

Protein sequence:

>479_residues
MAVFERVRAQLKARLGTEVYSSWFGRMKLAETSKGVVRISVPTAFLRAWINGHYLDLITSLWKAEDPSTLKVEIVVRTAT
RTSPVKADVKPVPARKASGQPQQALASVGVNGARVTPLPTAKADQAMRPSMFGSPLDSRFTFESFIEGASNRVAYAAART
VAELAAGAIRFNPLFIHASVGLGKTHLLQAVAAASLKRRPQSRVVYLTAEYFMWRFATAIRDNNALTLKEQLRDIDLLII
DDMQFLQGKSIQNEFCHLLNMLLDSARQVVVAADRPVSELESLEPRVKSRLNGGVSLEMLTPDYDLRLAMLKQRLACARV
EDPSLDIPLEVLEHVAQVVTGSGRELEGAFNQLVFRHSFEPQITLERIDEILGPVCRTGEPRRVRIEDIQRIVARHYNVS
KTELLSNRRTRSIVKPRQVAMYLAKVMTPRSLPEIGRRFGGRDHTTVLHAVRKIEGMSGDDKQLAQEIELLKRLISDQA

Sequences:

>Translated_479_residues
MAVFERVRAQLKARLGTEVYSSWFGRMKLAETSKGVVRISVPTAFLRAWINGHYLDLITSLWKAEDPSTLKVEIVVRTAT
RTSPVKADVKPVPARKASGQPQQALASVGVNGARVTPLPTAKADQAMRPSMFGSPLDSRFTFESFIEGASNRVAYAAART
VAELAAGAIRFNPLFIHASVGLGKTHLLQAVAAASLKRRPQSRVVYLTAEYFMWRFATAIRDNNALTLKEQLRDIDLLII
DDMQFLQGKSIQNEFCHLLNMLLDSARQVVVAADRPVSELESLEPRVKSRLNGGVSLEMLTPDYDLRLAMLKQRLACARV
EDPSLDIPLEVLEHVAQVVTGSGRELEGAFNQLVFRHSFEPQITLERIDEILGPVCRTGEPRRVRIEDIQRIVARHYNVS
KTELLSNRRTRSIVKPRQVAMYLAKVMTPRSLPEIGRRFGGRDHTTVLHAVRKIEGMSGDDKQLAQEIELLKRLISDQA
>Mature_478_residues
AVFERVRAQLKARLGTEVYSSWFGRMKLAETSKGVVRISVPTAFLRAWINGHYLDLITSLWKAEDPSTLKVEIVVRTATR
TSPVKADVKPVPARKASGQPQQALASVGVNGARVTPLPTAKADQAMRPSMFGSPLDSRFTFESFIEGASNRVAYAAARTV
AELAAGAIRFNPLFIHASVGLGKTHLLQAVAAASLKRRPQSRVVYLTAEYFMWRFATAIRDNNALTLKEQLRDIDLLIID
DMQFLQGKSIQNEFCHLLNMLLDSARQVVVAADRPVSELESLEPRVKSRLNGGVSLEMLTPDYDLRLAMLKQRLACARVE
DPSLDIPLEVLEHVAQVVTGSGRELEGAFNQLVFRHSFEPQITLERIDEILGPVCRTGEPRRVRIEDIQRIVARHYNVSK
TELLSNRRTRSIVKPRQVAMYLAKVMTPRSLPEIGRRFGGRDHTTVLHAVRKIEGMSGDDKQLAQEIELLKRLISDQA

Specific function: Plays an important role in the initiation and regulation of chromosomal replication. Binds to the origin of replication; it binds specifically double-stranded DNA at a 9 bp consensus (dnaA box):5'-TTATC[CA]A[CA]A-3'. DnaA binds to ATP and to acidic phosph

COG id: COG0593

COG function: function code L; ATPase involved in DNA replication initiation

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dnaA family [H]

Homologues:

Organism=Escherichia coli, GI2367267, Length=484, Percent_Identity=35.5371900826446, Blast_Score=278, Evalue=8e-76,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR001957
- InterPro:   IPR020591
- InterPro:   IPR018312
- InterPro:   IPR013159
- InterPro:   IPR013317
- InterPro:   IPR010921 [H]

Pfam domain/function: PF00308 Bac_DnaA; PF08299 Bac_DnaA_C [H]

EC number: NA

Molecular weight: Translated: 53521; Mature: 53390

Theoretical pI: Translated: 10.43; Mature: 10.43

Prosite motif: PS01008 DNAA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVFERVRAQLKARLGTEVYSSWFGRMKLAETSKGVVRISVPTAFLRAWINGHYLDLITS
CHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCEEEEECCHHHHHHHHCCHHHHHHHH
LWKAEDPSTLKVEIVVRTATRTSPVKADVKPVPARKASGQPQQALASVGVNGARVTPLPT
HHCCCCCCEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCEECCCCC
AKADQAMRPSMFGSPLDSRFTFESFIEGASNRVAYAAARTVAELAAGAIRFNPLFIHASV
CCCCHHCCCCCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHCCEEECEEEEEEEC
GLGKTHLLQAVAAASLKRRPQSRVVYLTAEYFMWRFATAIRDNNALTLKEQLRDIDLLII
CCCHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHCCCCEEHHHHHCCCCEEEE
DDMQFLQGKSIQNEFCHLLNMLLDSARQVVVAADRPVSELESLEPRVKSRLNGGVSLEML
CCHHHHCCCCHHHHHHHHHHHHHHHHCEEEEECCCCHHHHHHHCHHHHHHCCCCEEEEEE
TPDYDLRLAMLKQRLACARVEDPSLDIPLEVLEHVAQVVTGSGRELEGAFNQLVFRHSFE
CCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCC
PQITLERIDEILGPVCRTGEPRRVRIEDIQRIVARHYNVSKTELLSNRRTRSIVKPRQVA
CCEEHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHH
MYLAKVMTPRSLPEIGRRFGGRDHTTVLHAVRKIEGMSGDDKQLAQEIELLKRLISDQA
HHHHHHHCCCCCHHHHHHCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
AVFERVRAQLKARLGTEVYSSWFGRMKLAETSKGVVRISVPTAFLRAWINGHYLDLITS
HHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCEEEEECCHHHHHHHHCCHHHHHHHH
LWKAEDPSTLKVEIVVRTATRTSPVKADVKPVPARKASGQPQQALASVGVNGARVTPLPT
HHCCCCCCEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCEECCCCC
AKADQAMRPSMFGSPLDSRFTFESFIEGASNRVAYAAARTVAELAAGAIRFNPLFIHASV
CCCCHHCCCCCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHCCEEECEEEEEEEC
GLGKTHLLQAVAAASLKRRPQSRVVYLTAEYFMWRFATAIRDNNALTLKEQLRDIDLLII
CCCHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHCCCCEEHHHHHCCCCEEEE
DDMQFLQGKSIQNEFCHLLNMLLDSARQVVVAADRPVSELESLEPRVKSRLNGGVSLEML
CCHHHHCCCCHHHHHHHHHHHHHHHHCEEEEECCCCHHHHHHHCHHHHHHCCCCEEEEEE
TPDYDLRLAMLKQRLACARVEDPSLDIPLEVLEHVAQVVTGSGRELEGAFNQLVFRHSFE
CCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCC
PQITLERIDEILGPVCRTGEPRRVRIEDIQRIVARHYNVSKTELLSNRRTRSIVKPRQVA
CCEEHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHH
MYLAKVMTPRSLPEIGRRFGGRDHTTVLHAVRKIEGMSGDDKQLAQEIELLKRLISDQA
HHHHHHHCCCCCHHHHHHCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11214968 [H]