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The map label for this gene is bioB

Identifier: 109948197

GI number: 109948197

Start: 1504339

End: 1505187

Strand: Reverse

Name: bioB

Synonym: Hac_1730

Alternate gene names: 109948197

Gene position: 1505187-1504339 (Counterclockwise)

Preceding gene: 109948198

Following gene: 109948196

Centisome position: 96.86

GC content: 44.76

Gene sequence:

>849_bases
ATGCAAGAGATTTTTTTATGCTCCATTTCCAACGTGCGCAGTGGGGATTGCAAAGAAGATTGCGCTTATTGCACGCAAAG
CTCGCACCATCAAGGAGCGATCAAGCGCTATAAATTTAAAGATGAAAAAGTGGTTTTGCAAGAGGCTAGGGCGTTAAGGG
AGCTAGGGGCTTTAGGGTTTTGCTTGGTTACTTCAGGGCGCGAATTAGACGATGAAAAATGCGAATATATCGCTAAATTA
GCCAAAGCCATCAATCAAGAAGAATTGGGCTTGCATTTAATCGCATGCTGCGGGCGCGCGGATTTAGACCAACTAGAGTT
TTTAAGAGACGCAGGCATTCATAGCTATAACCATAATTTAGAGACTTCGCAAAATTTCTTCCCTAAAATTTGCTCCACGC
ACACATGGGAAGAAAGGTTTATCACATGCGAAAACGCTTTAAGGGCGGGCTTGGGGCTGTGCAGCGGGGGGATTTTTGGG
CTTAATGAGAGCTGGGAAGACAGGATTGAAATGCTTAGGGCGTTAGCTTCACTCTCCCCGCACACCACGCCGATTAACTT
TTTCATTAAAAACCCGGTTTTACCCATTGATGCAGAAACTTTAAGCGCGGATGAAGCCCTAGAATGCGTGCTTTTAGCCA
AAGAATTTTTGCCTAACGCTAGGCTTATGGCAGCTGGGGGGCGTGAAGTGGTGTTTAAAGATAACGATAAAAAGGAAGCC
AAGCTTTTTGAATACGGCATCAACGCAGTGGTGTTAGGGGACTATTTAACCACCAAAGGCAAAGCCCCTAAAAAAGATAT
AGAAAGACTGCTCTCTTATGGCTTGAAAATGGCGGCAAGCTGTCATTAA

Upstream 100 bases:

>100_bases
GCATTTTCTCTTTTAAACGCTTTTAAAATCAAAAGAGCTTCTTTTTCTTTGCTATAATCAGCCTAAAAAACCAACTTTTT
TAACACAACAAAGGACTGTT

Downstream 100 bases:

>100_bases
TGAGAGAACTTTTTAAAAGCATCAGAGGGTTTTTGTGCCTTCTTAAAATGATTTTCCCCCAGCGCTTGAAAAACGCCTTT
TGGGGGTTAAGCGAGTTGTT

Product: biotin synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 282; Mature: 282

Protein sequence:

>282_residues
MQEIFLCSISNVRSGDCKEDCAYCTQSSHHQGAIKRYKFKDEKVVLQEARALRELGALGFCLVTSGRELDDEKCEYIAKL
AKAINQEELGLHLIACCGRADLDQLEFLRDAGIHSYNHNLETSQNFFPKICSTHTWEERFITCENALRAGLGLCSGGIFG
LNESWEDRIEMLRALASLSPHTTPINFFIKNPVLPIDAETLSADEALECVLLAKEFLPNARLMAAGGREVVFKDNDKKEA
KLFEYGINAVVLGDYLTTKGKAPKKDIERLLSYGLKMAASCH

Sequences:

>Translated_282_residues
MQEIFLCSISNVRSGDCKEDCAYCTQSSHHQGAIKRYKFKDEKVVLQEARALRELGALGFCLVTSGRELDDEKCEYIAKL
AKAINQEELGLHLIACCGRADLDQLEFLRDAGIHSYNHNLETSQNFFPKICSTHTWEERFITCENALRAGLGLCSGGIFG
LNESWEDRIEMLRALASLSPHTTPINFFIKNPVLPIDAETLSADEALECVLLAKEFLPNARLMAAGGREVVFKDNDKKEA
KLFEYGINAVVLGDYLTTKGKAPKKDIERLLSYGLKMAASCH
>Mature_282_residues
MQEIFLCSISNVRSGDCKEDCAYCTQSSHHQGAIKRYKFKDEKVVLQEARALRELGALGFCLVTSGRELDDEKCEYIAKL
AKAINQEELGLHLIACCGRADLDQLEFLRDAGIHSYNHNLETSQNFFPKICSTHTWEERFITCENALRAGLGLCSGGIFG
LNESWEDRIEMLRALASLSPHTTPINFFIKNPVLPIDAETLSADEALECVLLAKEFLPNARLMAAGGREVVFKDNDKKEA
KLFEYGINAVVLGDYLTTKGKAPKKDIERLLSYGLKMAASCH

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family

Homologues:

Organism=Escherichia coli, GI1786992, Length=278, Percent_Identity=29.4964028776978, Blast_Score=144, Evalue=5e-36,
Organism=Saccharomyces cerevisiae, GI6321725, Length=286, Percent_Identity=31.4685314685315, Blast_Score=149, Evalue=5e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOB_HELAH (Q17VA0)

Other databases:

- EMBL:   AM260522
- RefSeq:   YP_665425.1
- ProteinModelPortal:   Q17VA0
- SMR:   Q17VA0
- STRING:   Q17VA0
- GeneID:   4177114
- GenomeReviews:   AM260522_GR
- KEGG:   hac:Hac_1730
- NMPDR:   fig|382638.8.peg.1673
- HOGENOM:   HBG405569
- OMA:   ATGATEF
- PhylomeDB:   Q17VA0
- ProtClustDB:   PRK08508
- BioCyc:   HACI382638:HAC_1730-MONOMER
- HAMAP:   MF_01694
- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00876
- SMART:   SM00729
- TIGRFAMs:   TIGR00433

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM

EC number: =2.8.1.6

Molecular weight: Translated: 31459; Mature: 31459

Theoretical pI: Translated: 5.88; Mature: 5.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.6 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
4.6 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQEIFLCSISNVRSGDCKEDCAYCTQSSHHQGAIKRYKFKDEKVVLQEARALRELGALGF
CCCEEEEECCCCCCCCCHHHHHHHHCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHCCCEE
CLVTSGRELDDEKCEYIAKLAKAINQEELGLHLIACCGRADLDQLEFLRDAGIHSYNHNL
EEEECCCCCCHHHHHHHHHHHHHHCHHHHCCHHEECCCCCCHHHHHHHHHCCCCCCCCCC
ETSQNFFPKICSTHTWEERFITCENALRAGLGLCSGGIFGLNESWEDRIEMLRALASLSP
CCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCC
HTTPINFFIKNPVLPIDAETLSADEALECVLLAKEFLPNARLMAAGGREVVFKDNDKKEA
CCCCEEEEECCCCCCCCHHHCCHHHHHHHHHHHHHHCCCCEEEECCCCEEEECCCCCHHH
KLFEYGINAVVLGDYLTTKGKAPKKDIERLLSYGLKMAASCH
HHHHHCCCEEEECCHHHCCCCCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MQEIFLCSISNVRSGDCKEDCAYCTQSSHHQGAIKRYKFKDEKVVLQEARALRELGALGF
CCCEEEEECCCCCCCCCHHHHHHHHCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHCCCEE
CLVTSGRELDDEKCEYIAKLAKAINQEELGLHLIACCGRADLDQLEFLRDAGIHSYNHNL
EEEECCCCCCHHHHHHHHHHHHHHCHHHHCCHHEECCCCCCHHHHHHHHHCCCCCCCCCC
ETSQNFFPKICSTHTWEERFITCENALRAGLGLCSGGIFGLNESWEDRIEMLRALASLSP
CCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCC
HTTPINFFIKNPVLPIDAETLSADEALECVLLAKEFLPNARLMAAGGREVVFKDNDKKEA
CCCCEEEEECCCCCCCCHHHCCHHHHHHHHHHHHHHCCCCEEEECCCCEEEECCCCCHHH
KLFEYGINAVVLGDYLTTKGKAPKKDIERLLSYGLKMAASCH
HHHHHCCCEEEECCHHHCCCCCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA