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The map label for this gene is purD [H]

Identifier: 109948077

GI number: 109948077

Start: 1389279

End: 1390553

Strand: Reverse

Name: purD [H]

Synonym: Hac_1595

Alternate gene names: 109948077

Gene position: 1390553-1389279 (Counterclockwise)

Preceding gene: 109948078

Following gene: 109948076

Centisome position: 89.49

GC content: 39.45

Gene sequence:

>1275_bases
ATGAAAGACAACAACAGCTATAATGTTTTAATTGTGGGGAATAAGGGGCGAGAGTATGCTTTAGCGCAAAGGCTTCAGCA
AGACGAACGAGTGAATGCTTTGTATTTTTGTTTGGGTAATGGTGGCACTCAAGATTTAGGTGAAAATTTGGAATGCGAAC
ATTACGAGCATGTCGTGGAATTAGCCCTTAAAAAACAAATCCATTTAGCCATCATTTCAGAAGAAGAGCCTTTAATTTTA
GGGCTTACAGAAATGCTAGAAAAAGCGAGTATTTTAGTGTTTGGGGCTTCTAAAGAGGTCGCTAAGCTAGAAACTTCTAA
AGGCTATATGAAAGCTTTCGCTAAAGAGTGCGGTATTAAAAGTGCATCTTATTTTGAAACAAGCGATCTCAAAGAAGCGT
TAAATTATCTCCAAAATGCCTCTTTCCCCTTAGTGGTTAAAGCGTCAAGCCAGCATGCAAGCGTTATCAACCATCAAGAA
GAAGCGCTAAAAGTCCTTGAAAGCGCTTTCAAACACAGCAATGAACCTGTGATCATAGAGCGTTTTTTAGAGGGCTTTGA
GCTTTCTGTCAGTGCACTAGTGGCCAATGACGATTTTATTTTGTTGCCCTTTTGTCAAAACTACAAACGCTTATTAGAGG
GGGATAATGGTGTCAATACTAGTGGCATGGGGGCGTTCGCTCCTGCAAACTTTTTCTTTGATCATTTAGAAGAAAAGATT
AAAGATCAGATTTTTAAACCCACTTTAAAAAAACTTCAAGCCAATCGTACGCCTTTTAAAGGGGTTTTACTCGCTGAAAT
CATGGTTGTAGAAGAAGAGGGCGTTTTAGAGCCGTATTTATTGGATTTTAGCACGCGTTTTGAAGACATGGAATGCCAGA
CGATTTTACCCCTTGTAGAAAATCCGCTTTTAGATTTGTTTTTAGCTACGACCAAAGGGGAATTAAACTCTCTTGAATTG
GTGTTTTCTAAAGAATTTGTGATGAGTGTGGCGCTTGTTTCTAGGAATTACCCCACTAGCTCTTCGCCCAAACAAACCCT
TTATATTGATCCGGTTGATGAAAAAAAGGGTCATTTGATTTTAGGGGAAGTCAATCAGGATAATGGCGTGTTTGAAAGCA
GTGGAGGGAGGGTGGCGTTTGCCATTGGTAAAGGGAAATCCTTATTAGAAGCTAGAAACCATGCGTATGAGATCGCTCAA
AAGGTGCATTTTGAAGGCATGTTTTATCGCAAGGATATTGGCTTTAAGGCGTTGGATTTGAAAGAATATTCTTAA

Upstream 100 bases:

>100_bases
AATAAGATTCTCCTAGCCTTATTGGTGTAGGTTATTGAACTTATGCACATGTAAAAGTAGCGGTCATCTGGGTTTAAAAC
AACCACATTTTAGGGAAGAC

Downstream 100 bases:

>100_bases
AAGTTTGAGACAAATGGAAATTTGTCTTGTTTGTTTTGAATGCGATCCCCAAATTTAGAAAAAGAAGAAACAGAAATCAT
AGAAACACTCCTTATGCATG

Product: glycinamide ribonucleotide synthetase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 424; Mature: 424

Protein sequence:

>424_residues
MKDNNSYNVLIVGNKGREYALAQRLQQDERVNALYFCLGNGGTQDLGENLECEHYEHVVELALKKQIHLAIISEEEPLIL
GLTEMLEKASILVFGASKEVAKLETSKGYMKAFAKECGIKSASYFETSDLKEALNYLQNASFPLVVKASSQHASVINHQE
EALKVLESAFKHSNEPVIIERFLEGFELSVSALVANDDFILLPFCQNYKRLLEGDNGVNTSGMGAFAPANFFFDHLEEKI
KDQIFKPTLKKLQANRTPFKGVLLAEIMVVEEEGVLEPYLLDFSTRFEDMECQTILPLVENPLLDLFLATTKGELNSLEL
VFSKEFVMSVALVSRNYPTSSSPKQTLYIDPVDEKKGHLILGEVNQDNGVFESSGGRVAFAIGKGKSLLEARNHAYEIAQ
KVHFEGMFYRKDIGFKALDLKEYS

Sequences:

>Translated_424_residues
MKDNNSYNVLIVGNKGREYALAQRLQQDERVNALYFCLGNGGTQDLGENLECEHYEHVVELALKKQIHLAIISEEEPLIL
GLTEMLEKASILVFGASKEVAKLETSKGYMKAFAKECGIKSASYFETSDLKEALNYLQNASFPLVVKASSQHASVINHQE
EALKVLESAFKHSNEPVIIERFLEGFELSVSALVANDDFILLPFCQNYKRLLEGDNGVNTSGMGAFAPANFFFDHLEEKI
KDQIFKPTLKKLQANRTPFKGVLLAEIMVVEEEGVLEPYLLDFSTRFEDMECQTILPLVENPLLDLFLATTKGELNSLEL
VFSKEFVMSVALVSRNYPTSSSPKQTLYIDPVDEKKGHLILGEVNQDNGVFESSGGRVAFAIGKGKSLLEARNHAYEIAQ
KVHFEGMFYRKDIGFKALDLKEYS
>Mature_424_residues
MKDNNSYNVLIVGNKGREYALAQRLQQDERVNALYFCLGNGGTQDLGENLECEHYEHVVELALKKQIHLAIISEEEPLIL
GLTEMLEKASILVFGASKEVAKLETSKGYMKAFAKECGIKSASYFETSDLKEALNYLQNASFPLVVKASSQHASVINHQE
EALKVLESAFKHSNEPVIIERFLEGFELSVSALVANDDFILLPFCQNYKRLLEGDNGVNTSGMGAFAPANFFFDHLEEKI
KDQIFKPTLKKLQANRTPFKGVLLAEIMVVEEEGVLEPYLLDFSTRFEDMECQTILPLVENPLLDLFLATTKGELNSLEL
VFSKEFVMSVALVSRNYPTSSSPKQTLYIDPVDEKKGHLILGEVNQDNGVFESSGGRVAFAIGKGKSLLEARNHAYEIAQ
KVHFEGMFYRKDIGFKALDLKEYS

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI28416899, Length=429, Percent_Identity=32.6340326340326, Blast_Score=215, Evalue=5e-56,
Organism=Homo sapiens, GI4503915, Length=429, Percent_Identity=32.6340326340326, Blast_Score=215, Evalue=6e-56,
Organism=Homo sapiens, GI209869995, Length=429, Percent_Identity=32.6340326340326, Blast_Score=215, Evalue=6e-56,
Organism=Homo sapiens, GI209869993, Length=429, Percent_Identity=32.6340326340326, Blast_Score=215, Evalue=6e-56,
Organism=Escherichia coli, GI1790438, Length=431, Percent_Identity=28.538283062645, Blast_Score=195, Evalue=6e-51,
Organism=Caenorhabditis elegans, GI17567511, Length=430, Percent_Identity=33.4883720930233, Blast_Score=200, Evalue=9e-52,
Organism=Saccharomyces cerevisiae, GI6321203, Length=448, Percent_Identity=30.5803571428571, Blast_Score=204, Evalue=2e-53,
Organism=Drosophila melanogaster, GI62471619, Length=438, Percent_Identity=30.1369863013699, Blast_Score=177, Evalue=1e-44,
Organism=Drosophila melanogaster, GI24582400, Length=442, Percent_Identity=30.0904977375566, Blast_Score=177, Evalue=1e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 47493; Mature: 47493

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDNNSYNVLIVGNKGREYALAQRLQQDERVNALYFCLGNGGTQDLGENLECEHYEHVVE
CCCCCCEEEEEECCCCCHHHHHHHHHHCCCCCEEEEEECCCCCHHHCCCCCHHHHHHHHH
LALKKQIHLAIISEEEPLILGLTEMLEKASILVFGASKEVAKLETSKGYMKAFAKECGIK
HHHHCCEEEEEECCCCCEEEEHHHHHHHCEEEEEECCCHHHHHHCCHHHHHHHHHHHCCC
SASYFETSDLKEALNYLQNASFPLVVKASSQHASVINHQEEALKVLESAFKHSNEPVIIE
CCCCCCHHHHHHHHHHHHCCCCCEEEEECCCHHHHHCCHHHHHHHHHHHHHCCCCCEEHH
RFLEGFELSVSALVANDDFILLPFCQNYKRLLEGDNGVNTSGMGAFAPANFFFDHLEEKI
HHHCCCCEEEEEEEECCCEEEECCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHH
KDQIFKPTLKKLQANRTPFKGVLLAEIMVVEEEGVLEPYLLDFSTRFEDMECQTILPLVE
HHHHHHHHHHHHHCCCCCCCCEEEEEEEEECCCCCCCCEEECHHCCCCCCCHHHHHHHHC
NPLLDLFLATTKGELNSLELVFSKEFVMSVALVSRNYPTSSSPKQTLYIDPVDEKKGHLI
CCHHHEEEECCCCCCCCEEEEEHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCEE
LGEVNQDNGVFESSGGRVAFAIGKGKSLLEARNHAYEIAQKVHFEGMFYRKDIGFKALDL
EEEECCCCCEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCEEEEECCCCEEECC
KEYS
CCCC
>Mature Secondary Structure
MKDNNSYNVLIVGNKGREYALAQRLQQDERVNALYFCLGNGGTQDLGENLECEHYEHVVE
CCCCCCEEEEEECCCCCHHHHHHHHHHCCCCCEEEEEECCCCCHHHCCCCCHHHHHHHHH
LALKKQIHLAIISEEEPLILGLTEMLEKASILVFGASKEVAKLETSKGYMKAFAKECGIK
HHHHCCEEEEEECCCCCEEEEHHHHHHHCEEEEEECCCHHHHHHCCHHHHHHHHHHHCCC
SASYFETSDLKEALNYLQNASFPLVVKASSQHASVINHQEEALKVLESAFKHSNEPVIIE
CCCCCCHHHHHHHHHHHHCCCCCEEEEECCCHHHHHCCHHHHHHHHHHHHHCCCCCEEHH
RFLEGFELSVSALVANDDFILLPFCQNYKRLLEGDNGVNTSGMGAFAPANFFFDHLEEKI
HHHCCCCEEEEEEEECCCEEEECCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHH
KDQIFKPTLKKLQANRTPFKGVLLAEIMVVEEEGVLEPYLLDFSTRFEDMECQTILPLVE
HHHHHHHHHHHHHCCCCCCCCEEEEEEEEECCCCCCCCEEECHHCCCCCCCHHHHHHHHC
NPLLDLFLATTKGELNSLELVFSKEFVMSVALVSRNYPTSSSPKQTLYIDPVDEKKGHLI
CCHHHEEEECCCCCCCCEEEEEHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCEE
LGEVNQDNGVFESSGGRVAFAIGKGKSLLEARNHAYEIAQKVHFEGMFYRKDIGFKALDL
EEEECCCCCEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCEEEEECCCCEEECC
KEYS
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]