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The map label for this gene is tuf

Identifier: 109948069

GI number: 109948069

Start: 1378418

End: 1379617

Strand: Reverse

Name: tuf

Synonym: Hac_1586

Alternate gene names: 109948069

Gene position: 1379617-1378418 (Counterclockwise)

Preceding gene: 109948071

Following gene: 109948068

Centisome position: 88.78

GC content: 43.33

Gene sequence:

>1200_bases
ATGGCAAAAGAAAAGTTTAACAGAACTAAGCCACATGTTAATATTGGGACCATTGGGCATGTAGACCATGGTAAAACAAC
TTTGAGTGCAGCGATCTCAGCGGTGCTTTCTTTGAAAGGTCTTGCAGAAATGAAAGACTATGACAATATTGATAACGCCC
CTGAAGAAAAAGAAAGAGGGATCACTATTGCTACTTCTCATATTGAATATGAGACTGAAAATAGGCACTATGCACATGTG
GATTGCCCAGGGCACGCCGACTATGTGAAAAACATGATTACAGGTGCGGCACAAATGGATGGAGCGATTTTAGTTGTTTC
TGCAGCTGATGGTCCTATGCCTCAAACTAGAGAGCATATCTTATTGTCTCGTCAAGTAGGCGTCCCTCACATCGTTGTTT
TCTTAAACAAACAAGACATGGTAGATGACCAAGAATTGTTAGAGCTTGTAGAAGTGGAAGTGCGTGAGTTATTGAGTGCG
TATGAATTCCCTGGTGATGATACTCCTATTGTAGCGGGTTCAGCTTTGAAAGCGTTAGAAGAAGCAAAAGCGGGTAGCGT
AGGTGAATGGGGCGAAAAAGTGCTCAAACTCATGGCTGAAGTGGATGCGTATATCCCTACTCCGGTGAGGGACACTGAAA
AAAGTTTCTTAATGCCGGTTGAAGATGTGTTTTCTATTGCAGGTAGGGGAACTGTGGTTACAGGTAGGATTGAAAGAGGC
ATGGTGAAAGTAGGCGATGAAGTGGAAATCGTTGGTATCAGAGCCACACAAAAAACAACCGTAACCGGCGTGGAAATGTT
TAGAAAAGAACTAGACAAAGGTGAAGCAGGCGATAATGTGGGCGTGCTTTTGAGAGGAACTAAAAAAGAAGAAGTAGAGC
GTGGTATGGTTTTGTGTAAGCCAGGTTCCATCACTCCGCACAAGAAATTTGAAGGGGAAATTTATGTCCTTTCTAAAGAA
GAAGGTGGGAGGCACACTCCATTCTTCACTAACTATCGTCCGCAATTCTATGTGCGCACGACTGATGTGACTGGATCGAT
CACCCTTCCTGAAGGCGTAGAAATGGTTATGCCTGGCGATAATGTGAAAATCACCGTAGAATTGATCAACTCTATTGCGT
TAGAGTTAGGGACAAAATTTGCGATCCGTGAAGGCGGTAGAACCGTTGGTGCTGGTGTTGTGAGCAATATTATTGAATAA

Upstream 100 bases:

>100_bases
ATGTCTTAAAATTTGTGGTAACATTGACAATATTCAGGCATATAAGGATTTTGTTTAGTATAATTCGAAAATCCATTTCA
AAAATTAAGGAGAAATACAA

Downstream 100 bases:

>100_bases
TATTAGCAAAAAGAGTTACCATAAAAAAGGGTCGTTATGAAAGTTAAAATAGGATTGAAGTGTTCTGATTGTGAAGACAT
CAATTACAGCACAACTAAGA

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu

Number of amino acids: Translated: 399; Mature: 398

Protein sequence:

>399_residues
MAKEKFNRTKPHVNIGTIGHVDHGKTTLSAAISAVLSLKGLAEMKDYDNIDNAPEEKERGITIATSHIEYETENRHYAHV
DCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLSRQVGVPHIVVFLNKQDMVDDQELLELVEVEVRELLSA
YEFPGDDTPIVAGSALKALEEAKAGSVGEWGEKVLKLMAEVDAYIPTPVRDTEKSFLMPVEDVFSIAGRGTVVTGRIERG
MVKVGDEVEIVGIRATQKTTVTGVEMFRKELDKGEAGDNVGVLLRGTKKEEVERGMVLCKPGSITPHKKFEGEIYVLSKE
EGGRHTPFFTNYRPQFYVRTTDVTGSITLPEGVEMVMPGDNVKITVELINSIALELGTKFAIREGGRTVGAGVVSNIIE

Sequences:

>Translated_399_residues
MAKEKFNRTKPHVNIGTIGHVDHGKTTLSAAISAVLSLKGLAEMKDYDNIDNAPEEKERGITIATSHIEYETENRHYAHV
DCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLSRQVGVPHIVVFLNKQDMVDDQELLELVEVEVRELLSA
YEFPGDDTPIVAGSALKALEEAKAGSVGEWGEKVLKLMAEVDAYIPTPVRDTEKSFLMPVEDVFSIAGRGTVVTGRIERG
MVKVGDEVEIVGIRATQKTTVTGVEMFRKELDKGEAGDNVGVLLRGTKKEEVERGMVLCKPGSITPHKKFEGEIYVLSKE
EGGRHTPFFTNYRPQFYVRTTDVTGSITLPEGVEMVMPGDNVKITVELINSIALELGTKFAIREGGRTVGAGVVSNIIE
>Mature_398_residues
AKEKFNRTKPHVNIGTIGHVDHGKTTLSAAISAVLSLKGLAEMKDYDNIDNAPEEKERGITIATSHIEYETENRHYAHVD
CPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLSRQVGVPHIVVFLNKQDMVDDQELLELVEVEVRELLSAY
EFPGDDTPIVAGSALKALEEAKAGSVGEWGEKVLKLMAEVDAYIPTPVRDTEKSFLMPVEDVFSIAGRGTVVTGRIERGM
VKVGDEVEIVGIRATQKTTVTGVEMFRKELDKGEAGDNVGVLLRGTKKEEVERGMVLCKPGSITPHKKFEGEIYVLSKEE
GGRHTPFFTNYRPQFYVRTTDVTGSITLPEGVEMVMPGDNVKITVELINSIALELGTKFAIREGGRTVGAGVVSNIIE

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily

Homologues:

Organism=Homo sapiens, GI34147630, Length=398, Percent_Identity=56.78391959799, Blast_Score=478, Evalue=1e-135,
Organism=Homo sapiens, GI223555963, Length=438, Percent_Identity=30.365296803653, Blast_Score=169, Evalue=4e-42,
Organism=Homo sapiens, GI5729864, Length=438, Percent_Identity=30.365296803653, Blast_Score=169, Evalue=4e-42,
Organism=Homo sapiens, GI4503471, Length=445, Percent_Identity=28.5393258426966, Blast_Score=144, Evalue=2e-34,
Organism=Homo sapiens, GI4503475, Length=450, Percent_Identity=29.1111111111111, Blast_Score=142, Evalue=6e-34,
Organism=Homo sapiens, GI54607086, Length=321, Percent_Identity=29.2834890965732, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI194018522, Length=335, Percent_Identity=28.0597014925373, Blast_Score=126, Evalue=5e-29,
Organism=Homo sapiens, GI194097354, Length=335, Percent_Identity=28.0597014925373, Blast_Score=125, Evalue=6e-29,
Organism=Homo sapiens, GI194018520, Length=337, Percent_Identity=28.1899109792285, Blast_Score=125, Evalue=6e-29,
Organism=Homo sapiens, GI46094014, Length=323, Percent_Identity=28.7925696594427, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI4503507, Length=273, Percent_Identity=29.6703296703297, Blast_Score=86, Evalue=5e-17,
Organism=Homo sapiens, GI82546879, Length=428, Percent_Identity=22.8971962616822, Blast_Score=69, Evalue=7e-12,
Organism=Homo sapiens, GI94966754, Length=135, Percent_Identity=34.8148148148148, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI157426893, Length=309, Percent_Identity=26.2135922330097, Blast_Score=66, Evalue=5e-11,
Organism=Escherichia coli, GI1789737, Length=398, Percent_Identity=73.6180904522613, Blast_Score=582, Evalue=1e-167,
Organism=Escherichia coli, GI1790412, Length=398, Percent_Identity=73.6180904522613, Blast_Score=582, Evalue=1e-167,
Organism=Escherichia coli, GI2367247, Length=340, Percent_Identity=31.4705882352941, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI48994988, Length=280, Percent_Identity=31.7857142857143, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1789108, Length=265, Percent_Identity=29.811320754717, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1790835, Length=150, Percent_Identity=30.6666666666667, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1788922, Length=336, Percent_Identity=25.297619047619, Blast_Score=63, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17556456, Length=405, Percent_Identity=49.8765432098765, Blast_Score=385, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI25141371, Length=390, Percent_Identity=43.8461538461538, Blast_Score=333, Evalue=9e-92,
Organism=Caenorhabditis elegans, GI32566629, Length=351, Percent_Identity=29.6296296296296, Blast_Score=138, Evalue=5e-33,
Organism=Caenorhabditis elegans, GI17552884, Length=454, Percent_Identity=27.5330396475771, Blast_Score=137, Evalue=7e-33,
Organism=Caenorhabditis elegans, GI17569207, Length=454, Percent_Identity=27.5330396475771, Blast_Score=137, Evalue=7e-33,
Organism=Caenorhabditis elegans, GI115532067, Length=447, Percent_Identity=27.7404921700224, Blast_Score=128, Evalue=5e-30,
Organism=Caenorhabditis elegans, GI115532065, Length=447, Percent_Identity=27.7404921700224, Blast_Score=128, Evalue=6e-30,
Organism=Caenorhabditis elegans, GI32566303, Length=445, Percent_Identity=26.2921348314607, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17506081, Length=304, Percent_Identity=27.3026315789474, Blast_Score=113, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI32566301, Length=156, Percent_Identity=35.2564102564103, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17557151, Length=237, Percent_Identity=26.1603375527426, Blast_Score=69, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6324761, Length=400, Percent_Identity=64.25, Blast_Score=523, Evalue=1e-149,
Organism=Saccharomyces cerevisiae, GI6325337, Length=445, Percent_Identity=28.7640449438202, Blast_Score=145, Evalue=1e-35,
Organism=Saccharomyces cerevisiae, GI6319594, Length=445, Percent_Identity=28.7640449438202, Blast_Score=145, Evalue=1e-35,
Organism=Saccharomyces cerevisiae, GI6322937, Length=324, Percent_Identity=24.6913580246914, Blast_Score=94, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6320863, Length=284, Percent_Identity=29.2253521126761, Blast_Score=89, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6320377, Length=345, Percent_Identity=29.8550724637681, Blast_Score=88, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6323098, Length=135, Percent_Identity=34.8148148148148, Blast_Score=72, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6323320, Length=241, Percent_Identity=28.6307053941909, Blast_Score=65, Evalue=1e-11,
Organism=Drosophila melanogaster, GI281363316, Length=396, Percent_Identity=57.3232323232323, Blast_Score=479, Evalue=1e-135,
Organism=Drosophila melanogaster, GI17864358, Length=396, Percent_Identity=57.3232323232323, Blast_Score=479, Evalue=1e-135,
Organism=Drosophila melanogaster, GI19921738, Length=390, Percent_Identity=51.025641025641, Blast_Score=413, Evalue=1e-116,
Organism=Drosophila melanogaster, GI24652838, Length=452, Percent_Identity=28.0973451327434, Blast_Score=140, Evalue=2e-33,
Organism=Drosophila melanogaster, GI17137572, Length=452, Percent_Identity=28.0973451327434, Blast_Score=140, Evalue=2e-33,
Organism=Drosophila melanogaster, GI45553807, Length=462, Percent_Identity=27.4891774891775, Blast_Score=139, Evalue=2e-33,
Organism=Drosophila melanogaster, GI45553816, Length=462, Percent_Identity=27.4891774891775, Blast_Score=139, Evalue=2e-33,
Organism=Drosophila melanogaster, GI24651721, Length=462, Percent_Identity=27.4891774891775, Blast_Score=139, Evalue=2e-33,
Organism=Drosophila melanogaster, GI17864154, Length=462, Percent_Identity=27.4891774891775, Blast_Score=139, Evalue=2e-33,
Organism=Drosophila melanogaster, GI17137380, Length=348, Percent_Identity=29.5977011494253, Blast_Score=134, Evalue=1e-31,
Organism=Drosophila melanogaster, GI19922690, Length=308, Percent_Identity=31.8181818181818, Blast_Score=132, Evalue=4e-31,
Organism=Drosophila melanogaster, GI45550900, Length=338, Percent_Identity=28.1065088757396, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24647046, Length=284, Percent_Identity=27.112676056338, Blast_Score=82, Evalue=5e-16,
Organism=Drosophila melanogaster, GI24647048, Length=284, Percent_Identity=27.112676056338, Blast_Score=82, Evalue=6e-16,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): EFTU_HELAH (Q17VM8)

Other databases:

- EMBL:   AM260522
- RefSeq:   YP_665297.1
- ProteinModelPortal:   Q17VM8
- SMR:   Q17VM8
- STRING:   Q17VM8
- GeneID:   4176676
- GenomeReviews:   AM260522_GR
- KEGG:   hac:Hac_1586
- NMPDR:   fig|382638.8.peg.1535
- eggNOG:   COG0050
- HOGENOM:   HBG307581
- OMA:   ERKPHIN
- PhylomeDB:   Q17VM8
- ProtClustDB:   PRK12735
- BioCyc:   HACI382638:HAC_1586-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00118_B
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000
- PANTHER:   PTHR23115:SF31
- PRINTS:   PR00315
- TIGRFAMs:   TIGR00485
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3; SSF50465 Elong_init_C; SSF50447 Translat_factor

EC number: 3.6.5.3

Molecular weight: Translated: 43540; Mature: 43409

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKEKFNRTKPHVNIGTIGHVDHGKTTLSAAISAVLSLKGLAEMKDYDNIDNAPEEKERG
CCCCCCCCCCCCEEECEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCC
ITIATSHIEYETENRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHI
EEEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHE
LLSRQVGVPHIVVFLNKQDMVDDQELLELVEVEVRELLSAYEFPGDDTPIVAGSALKALE
EEECCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEECHHHHHHHH
EAKAGSVGEWGEKVLKLMAEVDAYIPTPVRDTEKSFLMPVEDVFSIAGRGTVVTGRIERG
HHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCHHHHHHHCCCCEEEEEECCCC
MVKVGDEVEIVGIRATQKTTVTGVEMFRKELDKGEAGDNVGVLLRGTKKEEVERGMVLCK
EEEECCEEEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCEEEEECCCHHHHHCCEEEEC
PGSITPHKKFEGEIYVLSKEEGGRHTPFFTNYRPQFYVRTTDVTGSITLPEGVEMVMPGD
CCCCCCCCCCCCEEEEEECCCCCCCCCEEECCCCEEEEEEECCEEEEECCCCCEEEECCC
NVKITVELINSIALELGTKFAIREGGRTVGAGVVSNIIE
CCEEEHHHHHHHHHHHCCEEEECCCCCEEHHHHHHHHCC
>Mature Secondary Structure 
AKEKFNRTKPHVNIGTIGHVDHGKTTLSAAISAVLSLKGLAEMKDYDNIDNAPEEKERG
CCCCCCCCCCCEEECEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCC
ITIATSHIEYETENRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHI
EEEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHE
LLSRQVGVPHIVVFLNKQDMVDDQELLELVEVEVRELLSAYEFPGDDTPIVAGSALKALE
EEECCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEECHHHHHHHH
EAKAGSVGEWGEKVLKLMAEVDAYIPTPVRDTEKSFLMPVEDVFSIAGRGTVVTGRIERG
HHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCHHHHHHHCCCCEEEEEECCCC
MVKVGDEVEIVGIRATQKTTVTGVEMFRKELDKGEAGDNVGVLLRGTKKEEVERGMVLCK
EEEECCEEEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCEEEEECCCHHHHHCCEEEEC
PGSITPHKKFEGEIYVLSKEEGGRHTPFFTNYRPQFYVRTTDVTGSITLPEGVEMVMPGD
CCCCCCCCCCCCEEEEEECCCCCCCCCEEECCCCEEEEEEECCEEEEECCCCCEEEECCC
NVKITVELINSIALELGTKFAIREGGRTVGAGVVSNIIE
CCEEEHHHHHHHHHHHCCEEEECCCCCEEHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA