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The map label for this gene is hisS

Identifier: 109948052

GI number: 109948052

Start: 1358448

End: 1359776

Strand: Reverse

Name: hisS

Synonym: Hac_1566

Alternate gene names: 109948052

Gene position: 1359776-1358448 (Counterclockwise)

Preceding gene: 109948054

Following gene: 109948051

Centisome position: 87.51

GC content: 38.0

Gene sequence:

>1329_bases
ATGATTACCCCTAAAGTGTTGAGTGGGTTTAAAGACCGCTTGCCTAAAGATGCGATACAAAAGGCTCAGTTGCTTTCTAA
AGTTTCAGTTGTTTTTCAAAGTTTTGGTTTTGTGCCGATTGAAACCCCTCATTTGGAATACGCTGAAACGCTATTACCTG
ATGCGAGTAGCGATATTCAAAAAGAGATTTATCGCTTTAAAGATCATGGGGGTAGGGATGTGGCTTTAAGGTTTGATTTG
ACCGTGCCATTAGCGCGCTTTGTCTCTTTGTACCATCAAACGTTAGGAATGCCCTTTAAACGCTACGCCATAGGCAATGT
CTTTAGGGGTGAGAGGGCACAAAAAGGGCGTTATAGAGAATTCACGCAATGCGATTTTGATTTTATAGGGAGCGAGAGTT
TGGTGTGCGATGCTGAGATCATCCAAGTGGTTATCGCTTCCTTAAAAGCTTTGGATTTAGAAGATTTTTGCGTCTCTATC
AATCACAGGAAAATTTTGAATGGGATATGCGAATATTTTGGCATTTCTCAAGTGACTGGAGTGTTGCGCATTGTGGATAA
ATTAGAAAAGATTGGCTTAAATGGGGTTGAAGAAGAATTAAAAAAAGAGTGCGATTTAAATCCAAACACCATTAAAGGGC
TTTTAGAAATGGTTCAAATCAAGCAAGACGATTTAAGCCATGCGGAATTTTTTGAAAAAATTGCTTATTTGAAAGACTGT
AATGAAAATCTAAAAAAGGGCATACAGGATTTAGAAAAACTATACCAATTGCTAGGGGATTTACAAATTTCTCAAAACCT
GTATAAGATTGATTTTTCTATCGCTAGGGGCTTAGGGTATTATACAGGGATTGTGTATGAAACCACGCTTAATGACATGA
AGTCTTTAGGGAGCGTGTGTTCAGGGGGTCGTTATGATCATTTGACTAAAAATTTTTCTAAAGAAGATTTGCAAGGGGTG
GGGGCTTCTATTGGGATTGATAGATTGATTGTGGCTTTAAGTGGAATGCAATTATTAGATGAGCGTTCCACACAAGCTAA
AGTGCTAATCGCTTGCATGAATGAAGAATATTTTTCTTATGCAAACCGCTTGGCGGAGTCTTTAAGACAAAGCGGTATTT
TTAGCGAAGTCTATCCAGAAGCCCAAAAAATCAAAAAACCCTTTTCTTATGCTAACCATAAGGGGCATGAGTTTGTGGCC
ATCATTGGCGAAGAAGAATTTAAAAGCGAAACTTTAAGCTTAAAAAACATGCATTCAGGCATGCAACTGAATTGTTTGAG
CTTTTTAAAAGCCCTTGAAATCATTGGAGAAAACGATGAAGACTTATAA

Upstream 100 bases:

>100_bases
CAATAAAATGCGCATGCGTTTGGGTGCACTTACGCTCATCAATAGCCCTTTTTTAAAGATTTTAAGCTAAAATTTTAACA
TACAAATACAAGGAAATGGA

Downstream 100 bases:

>100_bases
TATCGCCATTGTTGGAGCTAGTGGGGCGGTAGGGCAAGAGTTAATTAAGGGTTTAGAAAATTCTTCTTTCCCTATTAAAA
AGTTTGTCCCGCTCGCTAGC

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase; HisRS

Number of amino acids: Translated: 442; Mature: 442

Protein sequence:

>442_residues
MITPKVLSGFKDRLPKDAIQKAQLLSKVSVVFQSFGFVPIETPHLEYAETLLPDASSDIQKEIYRFKDHGGRDVALRFDL
TVPLARFVSLYHQTLGMPFKRYAIGNVFRGERAQKGRYREFTQCDFDFIGSESLVCDAEIIQVVIASLKALDLEDFCVSI
NHRKILNGICEYFGISQVTGVLRIVDKLEKIGLNGVEEELKKECDLNPNTIKGLLEMVQIKQDDLSHAEFFEKIAYLKDC
NENLKKGIQDLEKLYQLLGDLQISQNLYKIDFSIARGLGYYTGIVYETTLNDMKSLGSVCSGGRYDHLTKNFSKEDLQGV
GASIGIDRLIVALSGMQLLDERSTQAKVLIACMNEEYFSYANRLAESLRQSGIFSEVYPEAQKIKKPFSYANHKGHEFVA
IIGEEEFKSETLSLKNMHSGMQLNCLSFLKALEIIGENDEDL

Sequences:

>Translated_442_residues
MITPKVLSGFKDRLPKDAIQKAQLLSKVSVVFQSFGFVPIETPHLEYAETLLPDASSDIQKEIYRFKDHGGRDVALRFDL
TVPLARFVSLYHQTLGMPFKRYAIGNVFRGERAQKGRYREFTQCDFDFIGSESLVCDAEIIQVVIASLKALDLEDFCVSI
NHRKILNGICEYFGISQVTGVLRIVDKLEKIGLNGVEEELKKECDLNPNTIKGLLEMVQIKQDDLSHAEFFEKIAYLKDC
NENLKKGIQDLEKLYQLLGDLQISQNLYKIDFSIARGLGYYTGIVYETTLNDMKSLGSVCSGGRYDHLTKNFSKEDLQGV
GASIGIDRLIVALSGMQLLDERSTQAKVLIACMNEEYFSYANRLAESLRQSGIFSEVYPEAQKIKKPFSYANHKGHEFVA
IIGEEEFKSETLSLKNMHSGMQLNCLSFLKALEIIGENDEDL
>Mature_442_residues
MITPKVLSGFKDRLPKDAIQKAQLLSKVSVVFQSFGFVPIETPHLEYAETLLPDASSDIQKEIYRFKDHGGRDVALRFDL
TVPLARFVSLYHQTLGMPFKRYAIGNVFRGERAQKGRYREFTQCDFDFIGSESLVCDAEIIQVVIASLKALDLEDFCVSI
NHRKILNGICEYFGISQVTGVLRIVDKLEKIGLNGVEEELKKECDLNPNTIKGLLEMVQIKQDDLSHAEFFEKIAYLKDC
NENLKKGIQDLEKLYQLLGDLQISQNLYKIDFSIARGLGYYTGIVYETTLNDMKSLGSVCSGGRYDHLTKNFSKEDLQGV
GASIGIDRLIVALSGMQLLDERSTQAKVLIACMNEEYFSYANRLAESLRQSGIFSEVYPEAQKIKKPFSYANHKGHEFVA
IIGEEEFKSETLSLKNMHSGMQLNCLSFLKALEIIGENDEDL

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI6996014, Length=436, Percent_Identity=32.7981651376147, Blast_Score=196, Evalue=3e-50,
Organism=Homo sapiens, GI15029520, Length=436, Percent_Identity=31.4220183486239, Blast_Score=186, Evalue=3e-47,
Organism=Homo sapiens, GI65287717, Length=414, Percent_Identity=26.0869565217391, Blast_Score=84, Evalue=3e-16,
Organism=Escherichia coli, GI1788861, Length=425, Percent_Identity=25.6470588235294, Blast_Score=116, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI71993693, Length=440, Percent_Identity=32.5, Blast_Score=202, Evalue=4e-52,
Organism=Caenorhabditis elegans, GI71993686, Length=440, Percent_Identity=32.5, Blast_Score=201, Evalue=5e-52,
Organism=Saccharomyces cerevisiae, GI6325290, Length=457, Percent_Identity=30.4157549234136, Blast_Score=181, Evalue=2e-46,
Organism=Saccharomyces cerevisiae, GI6320489, Length=312, Percent_Identity=23.0769230769231, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24643061, Length=462, Percent_Identity=29.8701298701299, Blast_Score=171, Evalue=7e-43,
Organism=Drosophila melanogaster, GI24643059, Length=480, Percent_Identity=28.3333333333333, Blast_Score=160, Evalue=2e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYH_HELAH (Q17VP5)

Other databases:

- EMBL:   AM260522
- RefSeq:   YP_665280.1
- ProteinModelPortal:   Q17VP5
- SMR:   Q17VP5
- STRING:   Q17VP5
- GeneID:   4177433
- GenomeReviews:   AM260522_GR
- KEGG:   hac:Hac_1566
- NMPDR:   fig|382638.8.peg.1516
- eggNOG:   COG0124
- HOGENOM:   HBG616575
- OMA:   IGKVFRD
- PhylomeDB:   Q17VP5
- ProtClustDB:   PRK00037
- BioCyc:   HACI382638:HAC_1566-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00127
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR015807
- InterPro:   IPR004516
- Gene3D:   G3DSA:3.40.50.800
- PANTHER:   PTHR11476
- PIRSF:   PIRSF001549
- TIGRFAMs:   TIGR00442

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; SSF52954 Anticodon_bd

EC number: =6.1.1.21

Molecular weight: Translated: 49920; Mature: 49920

Theoretical pI: Translated: 5.38; Mature: 5.38

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITPKVLSGFKDRLPKDAIQKAQLLSKVSVVFQSFGFVPIETPHLEYAETLLPDASSDIQ
CCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCHHHH
KEIYRFKDHGGRDVALRFDLTVPLARFVSLYHQTLGMPFKRYAIGNVFRGERAQKGRYRE
HHHHHHHCCCCCEEEEEEECHHHHHHHHHHHHHHHCCCHHHHHHCCHHCCCHHCCCCCCH
FTQCDFDFIGSESLVCDAEIIQVVIASLKALDLEDFCVSINHRKILNGICEYFGISQVTG
HHHCCCHHCCCCCCEEHHHHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHHHCHHHHHH
VLRIVDKLEKIGLNGVEEELKKECDLNPNTIKGLLEMVQIKQDDLSHAEFFEKIAYLKDC
HHHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
NENLKKGIQDLEKLYQLLGDLQISQNLYKIDFSIARGLGYYTGIVYETTLNDMKSLGSVC
HHHHHHHHHHHHHHHHHHCCHHHHCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
SGGRYDHLTKNFSKEDLQGVGASIGIDRLIVALSGMQLLDERSTQAKVLIACMNEEYFSY
CCCCCHHHHHCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCHHEEHHHHHCHHHHHH
ANRLAESLRQSGIFSEVYPEAQKIKKPFSYANHKGHEFVAIIGEEEFKSETLSLKNMHSG
HHHHHHHHHHCCCHHHHCCCHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHCCC
MQLNCLSFLKALEIIGENDEDL
CCHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MITPKVLSGFKDRLPKDAIQKAQLLSKVSVVFQSFGFVPIETPHLEYAETLLPDASSDIQ
CCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCHHHH
KEIYRFKDHGGRDVALRFDLTVPLARFVSLYHQTLGMPFKRYAIGNVFRGERAQKGRYRE
HHHHHHHCCCCCEEEEEEECHHHHHHHHHHHHHHHCCCHHHHHHCCHHCCCHHCCCCCCH
FTQCDFDFIGSESLVCDAEIIQVVIASLKALDLEDFCVSINHRKILNGICEYFGISQVTG
HHHCCCHHCCCCCCEEHHHHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHHHCHHHHHH
VLRIVDKLEKIGLNGVEEELKKECDLNPNTIKGLLEMVQIKQDDLSHAEFFEKIAYLKDC
HHHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
NENLKKGIQDLEKLYQLLGDLQISQNLYKIDFSIARGLGYYTGIVYETTLNDMKSLGSVC
HHHHHHHHHHHHHHHHHHCCHHHHCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
SGGRYDHLTKNFSKEDLQGVGASIGIDRLIVALSGMQLLDERSTQAKVLIACMNEEYFSY
CCCCCHHHHHCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCHHEEHHHHHCHHHHHH
ANRLAESLRQSGIFSEVYPEAQKIKKPFSYANHKGHEFVAIIGEEEFKSETLSLKNMHSG
HHHHHHHHHHCCCHHHHCCCHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHCCC
MQLNCLSFLKALEIIGENDEDL
CCHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA