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The map label for this gene is prfA

Identifier: 109948016

GI number: 109948016

Start: 1328955

End: 1330013

Strand: Reverse

Name: prfA

Synonym: Hac_1526

Alternate gene names: 109948016

Gene position: 1330013-1328955 (Counterclockwise)

Preceding gene: 109948017

Following gene: 109948015

Centisome position: 85.59

GC content: 40.32

Gene sequence:

>1059_bases
ATGTCCATTCTAGCCGAAAAGCTTTCTTCCATTCTCAAACGATACGACGAACTCACAGCCTTACTCTCTAGTGCTGAAGT
AATTAATGATATTAAAAAACTCACTGAATTGAGCAAAGAGCAAAGCTCTATTGAAGAAATTTCAGTGGCGAGTAAAGAGT
ATTTGAGCGTTTTAGAAAATATCAAAGAAAATAAAGAGCTTTTAGAAGACAAAGAATTGAGCGAACTCGCCAAAGAAGAA
TTGAAAATTTTAGAAATTAAAAAAAGCGAGCTAGAAACTACGATTAAGCAACTCCTTATCCCCAAAGATCCTAATGACGA
TAAAAATATCTATTTAGAATTAAGAGCTGGTACAGGGGGCGATGAAGCGGGCATTTTTGTAGGGGATTTGTTTAAGGCGT
ATTGCCGTTATGCAGATTTGAAAAAGTGGAAAGTAGAGATTGTGAGCTCTAGTGAAAATAGCGTAGGGGGCTATAAAGAA
ATCATCGCTTTGATTAAGGGCAAGGGCGTGTATTCAAGGCTCAAATTTGAAGCAGGCACGCACAGAGTCCAAAGAGTCCC
TGAGACAGAATCTCAAGGGCGTATCCACACTTCCGCTATCACGGTAGCGATCATGCCTGAAGTAGATGATGTGGAAATTT
CTATCAATCCTAGCGATTTAAAGATTGAAGTGTTTCGTGCTGGTGGGCATGGGGGGCAATGCGTCAATACCACAGACTCT
GCGGTTCGCATCACGCATCTCCCCACTAATATCAGCGTGAGCATGCAAGATGAAAAATCGCAGCATAAAAACAAGGACAA
AGCCCTAAAAATCTTAAAAGCACGCCTTTATGAAAAACAAATTGAAGAGCAACAACTCGCTAACGCTAAAGACAGAAAAG
AGCAAGTCGGCAGTGGGGATAGGAGCGAAAGGATCCGTACCTACAATTACCCGCAAAACCGCTTGAGCGAGCATAGAATC
AATTTAACTTTATATAGTTTAGAAGAAATCATGCTTTCAGGGAATTTAGATGAAGTGATCAACCCTTTAATCGCTCATGC
TCAAAGCCAGTTTGAATAA

Upstream 100 bases:

>100_bases
TCCTTTAGCTCATAATGAAATTTTATTATTGGGCTTCTTTTTAAGTTTTGCGTTTTTTAGGTTGTTGCATCTTTTTTTTT
AATTTTATAGGTAGTCTTGC

Downstream 100 bases:

>100_bases
AATCCCCACTAAGATAATCAAATAAAGTTAAAAAACCCATTTTTTTAAAATCCCGTTTAGGTTTCTAAAAATAGTCTATA
ATGCTCATAAGAGATATTTT

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1

Number of amino acids: Translated: 352; Mature: 351

Protein sequence:

>352_residues
MSILAEKLSSILKRYDELTALLSSAEVINDIKKLTELSKEQSSIEEISVASKEYLSVLENIKENKELLEDKELSELAKEE
LKILEIKKSELETTIKQLLIPKDPNDDKNIYLELRAGTGGDEAGIFVGDLFKAYCRYADLKKWKVEIVSSSENSVGGYKE
IIALIKGKGVYSRLKFEAGTHRVQRVPETESQGRIHTSAITVAIMPEVDDVEISINPSDLKIEVFRAGGHGGQCVNTTDS
AVRITHLPTNISVSMQDEKSQHKNKDKALKILKARLYEKQIEEQQLANAKDRKEQVGSGDRSERIRTYNYPQNRLSEHRI
NLTLYSLEEIMLSGNLDEVINPLIAHAQSQFE

Sequences:

>Translated_352_residues
MSILAEKLSSILKRYDELTALLSSAEVINDIKKLTELSKEQSSIEEISVASKEYLSVLENIKENKELLEDKELSELAKEE
LKILEIKKSELETTIKQLLIPKDPNDDKNIYLELRAGTGGDEAGIFVGDLFKAYCRYADLKKWKVEIVSSSENSVGGYKE
IIALIKGKGVYSRLKFEAGTHRVQRVPETESQGRIHTSAITVAIMPEVDDVEISINPSDLKIEVFRAGGHGGQCVNTTDS
AVRITHLPTNISVSMQDEKSQHKNKDKALKILKARLYEKQIEEQQLANAKDRKEQVGSGDRSERIRTYNYPQNRLSEHRI
NLTLYSLEEIMLSGNLDEVINPLIAHAQSQFE
>Mature_351_residues
SILAEKLSSILKRYDELTALLSSAEVINDIKKLTELSKEQSSIEEISVASKEYLSVLENIKENKELLEDKELSELAKEEL
KILEIKKSELETTIKQLLIPKDPNDDKNIYLELRAGTGGDEAGIFVGDLFKAYCRYADLKKWKVEIVSSSENSVGGYKEI
IALIKGKGVYSRLKFEAGTHRVQRVPETESQGRIHTSAITVAIMPEVDDVEISINPSDLKIEVFRAGGHGGQCVNTTDSA
VRITHLPTNISVSMQDEKSQHKNKDKALKILKARLYEKQIEEQQLANAKDRKEQVGSGDRSERIRTYNYPQNRLSEHRIN
LTLYSLEEIMLSGNLDEVINPLIAHAQSQFE

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family

Homologues:

Organism=Homo sapiens, GI166795303, Length=324, Percent_Identity=42.9012345679012, Blast_Score=261, Evalue=8e-70,
Organism=Homo sapiens, GI34577120, Length=320, Percent_Identity=40.3125, Blast_Score=234, Evalue=1e-61,
Organism=Homo sapiens, GI166795305, Length=225, Percent_Identity=41.3333333333333, Blast_Score=182, Evalue=4e-46,
Organism=Escherichia coli, GI1787462, Length=349, Percent_Identity=54.1547277936963, Blast_Score=386, Evalue=1e-108,
Organism=Escherichia coli, GI2367172, Length=337, Percent_Identity=37.0919881305638, Blast_Score=194, Evalue=8e-51,
Organism=Caenorhabditis elegans, GI17542784, Length=286, Percent_Identity=35.6643356643357, Blast_Score=179, Evalue=2e-45,
Organism=Saccharomyces cerevisiae, GI6321295, Length=311, Percent_Identity=45.016077170418, Blast_Score=244, Evalue=2e-65,
Organism=Drosophila melanogaster, GI19921226, Length=339, Percent_Identity=35.3982300884956, Blast_Score=211, Evalue=4e-55,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): RF1_HELAH (Q17VT1)

Other databases:

- EMBL:   AM260522
- RefSeq:   YP_665244.1
- ProteinModelPortal:   Q17VT1
- SMR:   Q17VT1
- STRING:   Q17VT1
- GeneID:   4176559
- GenomeReviews:   AM260522_GR
- KEGG:   hac:Hac_1526
- NMPDR:   fig|382638.8.peg.1478
- eggNOG:   COG0216
- HOGENOM:   HBG629764
- OMA:   NTNEGEH
- PhylomeDB:   Q17VT1
- ProtClustDB:   PRK00591
- BioCyc:   HACI382638:HAC_1526-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00093
- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373
- SMART:   SM00937
- TIGRFAMs:   TIGR00019

Pfam domain/function: PF03462 PCRF; PF00472 RF-1

EC number: NA

Molecular weight: Translated: 39673; Mature: 39541

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSILAEKLSSILKRYDELTALLSSAEVINDIKKLTELSKEQSSIEEISVASKEYLSVLEN
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IKENKELLEDKELSELAKEELKILEIKKSELETTIKQLLIPKDPNDDKNIYLELRAGTGG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCCC
DEAGIFVGDLFKAYCRYADLKKWKVEIVSSSENSVGGYKEIIALIKGKGVYSRLKFEAGT
CCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHCCCCHHHHHHHHHHH
HRVQRVPETESQGRIHTSAITVAIMPEVDDVEISINPSDLKIEVFRAGGHGGQCVNTTDS
HHHHCCCCCCCCCEEEEEEEEEEEECCCCCEEEEECCCCEEEEEEEECCCCCCEECCCCC
AVRITHLPTNISVSMQDEKSQHKNKDKALKILKARLYEKQIEEQQLANAKDRKEQVGSGD
EEEEEECCCCEEEEECCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCCC
RSERIRTYNYPQNRLSEHRINLTLYSLEEIMLSGNLDEVINPLIAHAQSQFE
CCCCCEECCCCHHHHHHHEEEEEEEHHHHHHHCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SILAEKLSSILKRYDELTALLSSAEVINDIKKLTELSKEQSSIEEISVASKEYLSVLEN
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IKENKELLEDKELSELAKEELKILEIKKSELETTIKQLLIPKDPNDDKNIYLELRAGTGG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCCC
DEAGIFVGDLFKAYCRYADLKKWKVEIVSSSENSVGGYKEIIALIKGKGVYSRLKFEAGT
CCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHCCCCHHHHHHHHHHH
HRVQRVPETESQGRIHTSAITVAIMPEVDDVEISINPSDLKIEVFRAGGHGGQCVNTTDS
HHHHCCCCCCCCCEEEEEEEEEEEECCCCCEEEEECCCCEEEEEEEECCCCCCEECCCCC
AVRITHLPTNISVSMQDEKSQHKNKDKALKILKARLYEKQIEEQQLANAKDRKEQVGSGD
EEEEEECCCCEEEEECCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCCC
RSERIRTYNYPQNRLSEHRINLTLYSLEEIMLSGNLDEVINPLIAHAQSQFE
CCCCCEECCCCHHHHHHHEEEEEEEHHHHHHHCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA