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The map label for this gene is radA [H]

Identifier: 109947988

GI number: 109947988

Start: 1298408

End: 1299754

Strand: Reverse

Name: radA [H]

Synonym: Hac_1496

Alternate gene names: 109947988

Gene position: 1299754-1298408 (Counterclockwise)

Preceding gene: 109947989

Following gene: 109947987

Centisome position: 83.64

GC content: 40.39

Gene sequence:

>1347_bases
ATGGCTAAAAAAACTTCTTTATTTGAGTGTCAGCATTGCGGTTTTACTAGCCCTAAATGGCTGGGTAAGTGCGTTCAATG
CAACTCATGGGAGAGCTTTATTGAATTAAACCAAACCCAAAAAGAAGTTTTAAACACGCTTAAAAACCCCCTTTCAAAAA
TACAAAAAAGCGTTTCTATTGTGGAAATTGAGCATGAAGAAGTGGTGAAATTTTCTTCCACTCAAAGCGAATTAGACATT
GTTTTAGGTGGGGGGATTGCTAAAGGGGGGCTGTATTTAGTAGGGGGGAGTCCTGGGGTGGGGAAATCCACTTTGCTTTT
AAAAGTGGCTTCTGGGTTAGCCAAAAACCAGCAAAAGGTTTTGTATGTGAGCGGTGAAGAGAGCTTGAGCCAGATTAAAA
TGCGAGCCACACGATTAGATTGCATAGAAAAAGAATTGTATTTGCTTAATGAAATCAATTGGCCTGTGATTAAATCCAAT
ATTGAAAGCGAGGATTATTTTGCTTGCGTGATTGATTCTATTCAAACGCTTTATTCGCCAGAGATTTCTTCAGCACCCGG
TTCTATTTCGCAAGTGCGAGAAATCACTTTTGAGCTCATGCGTTTGGCAAAGACAAGAGACATCGCTATTTTTATCATCG
GTCATATCACTAAAGAAGGGAGTATTGCAGGCCCTAGAGTGTTAGAGCATATGGTAGATAGCGTGCTGTATTTTGAAGGC
GATCCGAGTAGGGAATTAAGGATTTTAAGGAGTTTTAAAAACCGCTTTGGCCCTACGAGTGAGATTGGGTTGTTTGAAAT
GAAAGAGCAGGGTTTGGTGAGCGCTAAAGAGGCTTCAAGCTTGTTTTTTTCTAAAGAAGAGCCTATGGAGGGAAGTGCGA
TTACCATTACTTTAGAAGGTTCAAGGGCGTTGATTTTAGAAATTCAGGCGTTAGTGAGCGAGTGCAGTTTTGGAGTGCCC
AAACGATTGGCGAATGGGTTTGACACTAACCGCCTTAACATGCTCATCGCTTTGTTAGAAAAAAAGCTAGAAATCCCTTT
AAATCGCCATGATGTGTTTATTAATGTGAGTGGGGGCATTAAAATCAGCGAGCCGGCTTGCGATTTAGCCGTCATTGCCA
GCATCCTTTCAAGCTTTAAAAACAGAAAAATTGACAATAAAACCGCCTTTTTGGGTGAAGTGAGTTTGAATGGTAGGATT
TTAGAAGCCCCTAATTTGAACGCCAGATTGAAAGAAATGGAAAATTATGGGTTTTTAAAAGCCATTTTGCCTAAAAAACC
CAGTCAAAAAACTTCTATCAAGTGCTATGAAGCCAATGTGGTGGGTAAGATTGTTGAGTGGATGTAG

Upstream 100 bases:

>100_bases
CATGAGCGCGACTACCGGAACTTTATTTGCCATTGAAAACGCCCTGCAAGAATTGCTGGACCGCAATATCAGGGTGTTAC
CGATTTAAACGGCAATCGCT

Downstream 100 bases:

>100_bases
GTTTAAAGCTCTATTTTTAGCATTTTTTACAATCTAATATCAAAGAGCTTGATTTTCATCAAGCGATAACCCCTTGAAAA
AAGCAGTCTTGGATTAGGGG

Product: DNA repair protein RadA

Products: NA

Alternate protein names: DNA repair protein sms homolog [H]

Number of amino acids: Translated: 448; Mature: 447

Protein sequence:

>448_residues
MAKKTSLFECQHCGFTSPKWLGKCVQCNSWESFIELNQTQKEVLNTLKNPLSKIQKSVSIVEIEHEEVVKFSSTQSELDI
VLGGGIAKGGLYLVGGSPGVGKSTLLLKVASGLAKNQQKVLYVSGEESLSQIKMRATRLDCIEKELYLLNEINWPVIKSN
IESEDYFACVIDSIQTLYSPEISSAPGSISQVREITFELMRLAKTRDIAIFIIGHITKEGSIAGPRVLEHMVDSVLYFEG
DPSRELRILRSFKNRFGPTSEIGLFEMKEQGLVSAKEASSLFFSKEEPMEGSAITITLEGSRALILEIQALVSECSFGVP
KRLANGFDTNRLNMLIALLEKKLEIPLNRHDVFINVSGGIKISEPACDLAVIASILSSFKNRKIDNKTAFLGEVSLNGRI
LEAPNLNARLKEMENYGFLKAILPKKPSQKTSIKCYEANVVGKIVEWM

Sequences:

>Translated_448_residues
MAKKTSLFECQHCGFTSPKWLGKCVQCNSWESFIELNQTQKEVLNTLKNPLSKIQKSVSIVEIEHEEVVKFSSTQSELDI
VLGGGIAKGGLYLVGGSPGVGKSTLLLKVASGLAKNQQKVLYVSGEESLSQIKMRATRLDCIEKELYLLNEINWPVIKSN
IESEDYFACVIDSIQTLYSPEISSAPGSISQVREITFELMRLAKTRDIAIFIIGHITKEGSIAGPRVLEHMVDSVLYFEG
DPSRELRILRSFKNRFGPTSEIGLFEMKEQGLVSAKEASSLFFSKEEPMEGSAITITLEGSRALILEIQALVSECSFGVP
KRLANGFDTNRLNMLIALLEKKLEIPLNRHDVFINVSGGIKISEPACDLAVIASILSSFKNRKIDNKTAFLGEVSLNGRI
LEAPNLNARLKEMENYGFLKAILPKKPSQKTSIKCYEANVVGKIVEWM
>Mature_447_residues
AKKTSLFECQHCGFTSPKWLGKCVQCNSWESFIELNQTQKEVLNTLKNPLSKIQKSVSIVEIEHEEVVKFSSTQSELDIV
LGGGIAKGGLYLVGGSPGVGKSTLLLKVASGLAKNQQKVLYVSGEESLSQIKMRATRLDCIEKELYLLNEINWPVIKSNI
ESEDYFACVIDSIQTLYSPEISSAPGSISQVREITFELMRLAKTRDIAIFIIGHITKEGSIAGPRVLEHMVDSVLYFEGD
PSRELRILRSFKNRFGPTSEIGLFEMKEQGLVSAKEASSLFFSKEEPMEGSAITITLEGSRALILEIQALVSECSFGVPK
RLANGFDTNRLNMLIALLEKKLEIPLNRHDVFINVSGGIKISEPACDLAVIASILSSFKNRKIDNKTAFLGEVSLNGRIL
EAPNLNARLKEMENYGFLKAILPKKPSQKTSIKCYEANVVGKIVEWM

Specific function: May play a role in the repair of endogenous alkylation damage [H]

COG id: COG1066

COG function: function code O; Predicted ATP-dependent serine protease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recA family. RadA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790850, Length=426, Percent_Identity=41.0798122065728, Blast_Score=336, Evalue=1e-93,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR014774
- InterPro:   IPR004504
- InterPro:   IPR008269
- InterPro:   IPR020568 [H]

Pfam domain/function: PF06745 KaiC; PF05362 Lon_C [H]

EC number: NA

Molecular weight: Translated: 49633; Mature: 49502

Theoretical pI: Translated: 7.67; Mature: 7.67

Prosite motif: PS50162 RECA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKKTSLFECQHCGFTSPKWLGKCVQCNSWESFIELNQTQKEVLNTLKNPLSKIQKSVSI
CCCCCCCHHHHCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEE
VEIEHEEVVKFSSTQSELDIVLGGGIAKGGLYLVGGSPGVGKSTLLLKVASGLAKNQQKV
EEECHHHHHHHCCCCCCEEEEEECCCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCEE
LYVSGEESLSQIKMRATRLDCIEKELYLLNEINWPVIKSNIESEDYFACVIDSIQTLYSP
EEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCHHHHHHHHHHHHHCCC
EISSAPGSISQVREITFELMRLAKTRDIAIFIIGHITKEGSIAGPRVLEHMVDSVLYFEG
CCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHEEEECC
DPSRELRILRSFKNRFGPTSEIGLFEMKEQGLVSAKEASSLFFSKEEPMEGSAITITLEG
CCHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCHHHHHHHHCCCCCCCCCCEEEEEECC
SRALILEIQALVSECSFGVPKRLANGFDTNRLNMLIALLEKKLEIPLNRHDVFINVSGGI
CCEEEHHHHHHHHHHCCCCCHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEECCCE
KISEPACDLAVIASILSSFKNRKIDNKTAFLGEVSLNGRILEAPNLNARLKEMENYGFLK
EECCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEEECCCEEEECCCCCHHHHHHHCCCCEE
AILPKKPSQKTSIKCYEANVVGKIVEWM
ECCCCCCCCCCEEEEEEHHHHHHHHHCC
>Mature Secondary Structure 
AKKTSLFECQHCGFTSPKWLGKCVQCNSWESFIELNQTQKEVLNTLKNPLSKIQKSVSI
CCCCCCHHHHCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEE
VEIEHEEVVKFSSTQSELDIVLGGGIAKGGLYLVGGSPGVGKSTLLLKVASGLAKNQQKV
EEECHHHHHHHCCCCCCEEEEEECCCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCEE
LYVSGEESLSQIKMRATRLDCIEKELYLLNEINWPVIKSNIESEDYFACVIDSIQTLYSP
EEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCHHHHHHHHHHHHHCCC
EISSAPGSISQVREITFELMRLAKTRDIAIFIIGHITKEGSIAGPRVLEHMVDSVLYFEG
CCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHEEEECC
DPSRELRILRSFKNRFGPTSEIGLFEMKEQGLVSAKEASSLFFSKEEPMEGSAITITLEG
CCHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCHHHHHHHHCCCCCCCCCCEEEEEECC
SRALILEIQALVSECSFGVPKRLANGFDTNRLNMLIALLEKKLEIPLNRHDVFINVSGGI
CCEEEHHHHHHHHHHCCCCCHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEECCCE
KISEPACDLAVIASILSSFKNRKIDNKTAFLGEVSLNGRILEAPNLNARLKEMENYGFLK
EECCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEEECCCEEEECCCCCHHHHHHHCCCCEE
AILPKKPSQKTSIKCYEANVVGKIVEWM
ECCCCCCCCCCEEEEEEHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]