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The map label for this gene is serA [C]

Identifier: 109947976

GI number: 109947976

Start: 1290829

End: 1291773

Strand: Reverse

Name: serA [C]

Synonym: Hac_1481

Alternate gene names: 109947976

Gene position: 1291773-1290829 (Counterclockwise)

Preceding gene: 109947978

Following gene: 109947973

Centisome position: 83.13

GC content: 38.73

Gene sequence:

>945_bases
ATGAAAACATTTAAAAAAGGCGTGGTGCTAGACGCTAAAAGCGTGGGGTTAAAAGCGCTAGAGGTTTTAAAAGAGGTGGC
GGATTTTCATTTTTATGAAACCACTTTGCCCAGTCAAATCATTGAACGATCCATTGATGCTGAAATTATGGTATTGAATA
AAGTGGTGATCACTAAAGAAATTTTAACGCAATTGCCCAAGCTTAAGCTCATTTGCATCACTGCCACAGGCATGGATAAT
GTGGATGTGAAAAGCGCGAAAGATTTAGGTATAGAAGTCAAAAATGTGAGCGCTTATTCTACTGAGTCCGTAGCCCAGCA
CACTTTAGCGTGCGCGTTGTCTTTGTTGGGGAGGATCAATTATTACGATCATTATTGCAAAAGCGGGGAATATAGTCAAA
GCGATATTTTTACGCATATTAGTGATATTAAAATGGGGCTTATTAAGGGGGGTCAATGGGGGGTTATTGGTTTAGGCGCT
ATCGGTAAAAGAGTCGCCAAACTCGCTCAAGCTTTTGGGGCAAAGGTGGTGTATTATTCCCCTAAAGACAAAAAAGAAGA
GTATGAGTGCTTGAGTTTGGAAAATTTACTCCAAACAAGCGATATTGTTAGCATTCATGCTCCTTTGAATGAAAGCACAC
GCAATTTGATCGCTCTAAAAGAATTGCAAAGCCTAAAAGAAGGGGCGATTTTAATCAATGTGGGGCGTGGGGGCATTGTG
AATGAAAAGGATTTGGCTTTGGTGTTAGAAACCAAGGATTTGTATTACGCAAGCGATGTGTTTGTTAAAGAGCCTTTTGA
AAAAGATCATGTTTTTTTAAACCCAAAGATCCAAAACAAATTGCTTTTAACCCCTCATATCGCATGGGCGTATAGCGATA
GCTTGAAAACCTTGATAGAAAAAACCAAAGAAAATATCCAAGATTTTTTAACTTCTCAAAAATAA

Upstream 100 bases:

>100_bases
GGGAGGGTGTTTTTCTTGCTTTTTGGCATGTTTAGTTTCTTTAAAAAGGGATTTTGTGGTTTAATTATATAACTTTAAAA
TTAAATCTAGGAGTAAAGAG

Downstream 100 bases:

>100_bases
ACGCAAAAAAAGGGGGGTTTAAGCGTTTGGGTTTCTTTACTAACGCTTAATGTTTTTTGTGTGCAGCAAAAATTAAAATG
CTTTTAAATTTTTAAGGTTC

Product: 2-hydroxyacid dehydrogenase

Products: 3-phosphohydroxypyruvate; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 314; Mature: 314

Protein sequence:

>314_residues
MKTFKKGVVLDAKSVGLKALEVLKEVADFHFYETTLPSQIIERSIDAEIMVLNKVVITKEILTQLPKLKLICITATGMDN
VDVKSAKDLGIEVKNVSAYSTESVAQHTLACALSLLGRINYYDHYCKSGEYSQSDIFTHISDIKMGLIKGGQWGVIGLGA
IGKRVAKLAQAFGAKVVYYSPKDKKEEYECLSLENLLQTSDIVSIHAPLNESTRNLIALKELQSLKEGAILINVGRGGIV
NEKDLALVLETKDLYYASDVFVKEPFEKDHVFLNPKIQNKLLLTPHIAWAYSDSLKTLIEKTKENIQDFLTSQK

Sequences:

>Translated_314_residues
MKTFKKGVVLDAKSVGLKALEVLKEVADFHFYETTLPSQIIERSIDAEIMVLNKVVITKEILTQLPKLKLICITATGMDN
VDVKSAKDLGIEVKNVSAYSTESVAQHTLACALSLLGRINYYDHYCKSGEYSQSDIFTHISDIKMGLIKGGQWGVIGLGA
IGKRVAKLAQAFGAKVVYYSPKDKKEEYECLSLENLLQTSDIVSIHAPLNESTRNLIALKELQSLKEGAILINVGRGGIV
NEKDLALVLETKDLYYASDVFVKEPFEKDHVFLNPKIQNKLLLTPHIAWAYSDSLKTLIEKTKENIQDFLTSQK
>Mature_314_residues
MKTFKKGVVLDAKSVGLKALEVLKEVADFHFYETTLPSQIIERSIDAEIMVLNKVVITKEILTQLPKLKLICITATGMDN
VDVKSAKDLGIEVKNVSAYSTESVAQHTLACALSLLGRINYYDHYCKSGEYSQSDIFTHISDIKMGLIKGGQWGVIGLGA
IGKRVAKLAQAFGAKVVYYSPKDKKEEYECLSLENLLQTSDIVSIHAPLNESTRNLIALKELQSLKEGAILINVGRGGIV
NEKDLALVLETKDLYYASDVFVKEPFEKDHVFLNPKIQNKLLLTPHIAWAYSDSLKTLIEKTKENIQDFLTSQK

Specific function: Serine biosynthesis; first step. [C]

COG id: COG1052

COG function: function code CHR; Lactate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI4557497, Length=317, Percent_Identity=33.1230283911672, Blast_Score=148, Evalue=6e-36,
Organism=Homo sapiens, GI61743967, Length=316, Percent_Identity=33.2278481012658, Blast_Score=148, Evalue=7e-36,
Organism=Homo sapiens, GI145580578, Length=317, Percent_Identity=30.9148264984227, Blast_Score=137, Evalue=1e-32,
Organism=Homo sapiens, GI4557499, Length=317, Percent_Identity=30.9148264984227, Blast_Score=137, Evalue=1e-32,
Organism=Homo sapiens, GI145580575, Length=298, Percent_Identity=32.2147651006711, Blast_Score=136, Evalue=2e-32,
Organism=Homo sapiens, GI23308577, Length=247, Percent_Identity=28.3400809716599, Blast_Score=99, Evalue=5e-21,
Organism=Homo sapiens, GI6912396, Length=273, Percent_Identity=26.007326007326, Blast_Score=80, Evalue=3e-15,
Organism=Escherichia coli, GI1789279, Length=286, Percent_Identity=30.0699300699301, Blast_Score=107, Evalue=9e-25,
Organism=Escherichia coli, GI1787645, Length=270, Percent_Identity=25.1851851851852, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI87082289, Length=289, Percent_Identity=27.3356401384083, Blast_Score=98, Evalue=6e-22,
Organism=Caenorhabditis elegans, GI17532191, Length=269, Percent_Identity=33.457249070632, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI25147481, Length=309, Percent_Identity=28.8025889967638, Blast_Score=115, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6324964, Length=304, Percent_Identity=26.3157894736842, Blast_Score=102, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6322116, Length=306, Percent_Identity=25.4901960784314, Blast_Score=91, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6320925, Length=306, Percent_Identity=25.4901960784314, Blast_Score=91, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6324055, Length=285, Percent_Identity=25.2631578947368, Blast_Score=86, Evalue=6e-18,
Organism=Saccharomyces cerevisiae, GI6324980, Length=138, Percent_Identity=30.4347826086957, Blast_Score=63, Evalue=6e-11,
Organism=Drosophila melanogaster, GI24646446, Length=304, Percent_Identity=32.8947368421053, Blast_Score=140, Evalue=8e-34,
Organism=Drosophila melanogaster, GI24646448, Length=304, Percent_Identity=32.8947368421053, Blast_Score=140, Evalue=8e-34,
Organism=Drosophila melanogaster, GI24646452, Length=304, Percent_Identity=32.8947368421053, Blast_Score=140, Evalue=8e-34,
Organism=Drosophila melanogaster, GI24646450, Length=304, Percent_Identity=32.8947368421053, Blast_Score=140, Evalue=8e-34,
Organism=Drosophila melanogaster, GI62472511, Length=305, Percent_Identity=33.1147540983607, Blast_Score=140, Evalue=1e-33,
Organism=Drosophila melanogaster, GI28574286, Length=302, Percent_Identity=27.4834437086093, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI19921140, Length=285, Percent_Identity=27.719298245614, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24585516, Length=242, Percent_Identity=28.9256198347107, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI28571528, Length=314, Percent_Identity=27.0700636942675, Blast_Score=79, Evalue=5e-15,
Organism=Drosophila melanogaster, GI45552429, Length=237, Percent_Identity=27.0042194092827, Blast_Score=72, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24585514, Length=233, Percent_Identity=27.8969957081545, Blast_Score=72, Evalue=5e-13,
Organism=Drosophila melanogaster, GI28574282, Length=233, Percent_Identity=27.8969957081545, Blast_Score=72, Evalue=5e-13,
Organism=Drosophila melanogaster, GI28574284, Length=233, Percent_Identity=27.8969957081545, Blast_Score=72, Evalue=5e-13,
Organism=Drosophila melanogaster, GI45551003, Length=233, Percent_Identity=27.8969957081545, Blast_Score=72, Evalue=5e-13,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: 1.1.1.95

Molecular weight: Translated: 34925; Mature: 34925

Theoretical pI: Translated: 7.57; Mature: 7.57

Prosite motif: PS00671 D_2_HYDROXYACID_DH_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTFKKGVVLDAKSVGLKALEVLKEVADFHFYETTLPSQIIERSIDAEIMVLNKVVITKE
CCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCEEEEHHHHHHHHH
ILTQLPKLKLICITATGMDNVDVKSAKDLGIEVKNVSAYSTESVAQHTLACALSLLGRIN
HHHHCCCEEEEEEEECCCCCCCCCCHHHCCEEEECCCCCCHHHHHHHHHHHHHHHHHHCC
YYDHYCKSGEYSQSDIFTHISDIKMGLIKGGQWGVIGLGAIGKRVAKLAQAFGAKVVYYS
HHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHCCEEEEEC
PKDKKEEYECLSLENLLQTSDIVSIHAPLNESTRNLIALKELQSLKEGAILINVGRGGIV
CCCCCHHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHCCEEEEECCCCCCC
NEKDLALVLETKDLYYASDVFVKEPFEKDHVFLNPKIQNKLLLTPHIAWAYSDSLKTLIE
CCCCEEEEEEECCCEEECCHHEECCCCCCCEEECCCCCCEEEECCCCHHHHHHHHHHHHH
KTKENIQDFLTSQK
HHHHHHHHHHHCCC
>Mature Secondary Structure
MKTFKKGVVLDAKSVGLKALEVLKEVADFHFYETTLPSQIIERSIDAEIMVLNKVVITKE
CCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCEEEEHHHHHHHHH
ILTQLPKLKLICITATGMDNVDVKSAKDLGIEVKNVSAYSTESVAQHTLACALSLLGRIN
HHHHCCCEEEEEEEECCCCCCCCCCHHHCCEEEECCCCCCHHHHHHHHHHHHHHHHHHCC
YYDHYCKSGEYSQSDIFTHISDIKMGLIKGGQWGVIGLGAIGKRVAKLAQAFGAKVVYYS
HHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHCCEEEEEC
PKDKKEEYECLSLENLLQTSDIVSIHAPLNESTRNLIALKELQSLKEGAILINVGRGGIV
CCCCCHHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHCCEEEEECCCCCCC
NEKDLALVLETKDLYYASDVFVKEPFEKDHVFLNPKIQNKLLLTPHIAWAYSDSLKTLIE
CCCCEEEEEEECCCEEECCHHEECCCCCCCEEECCCCCCEEEECCCCHHHHHHHHHHHHH
KTKENIQDFLTSQK
HHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-phosphoglycerate; NAD+

Specific reaction: 3-phosphoglycerate + NAD+ = 3-phosphohydroxypyruvate + NADH + H+

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800; 10675023 [H]