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The map label for this gene is grpE [H]

Identifier: 109947964

GI number: 109947964

Start: 1275301

End: 1275861

Strand: Reverse

Name: grpE [H]

Synonym: Hac_1469

Alternate gene names: 109947964

Gene position: 1275861-1275301 (Counterclockwise)

Preceding gene: 109947965

Following gene: 109947963

Centisome position: 82.11

GC content: 39.57

Gene sequence:

>561_bases
ATGAAAGATGAACACAACCAAGAACACGATTTAAGCCAAAAAGAGCTAGAGTCTTGCGAAAATTCTTGCACTTGTGAAGG
AAAAAAACAAGAAGCGAGCGAAAAAGAATGCGAGATTAAAGAAGATTTTGAGCTTAAATACCAAGAAATGCACGAAAAAT
ATTTGAGGGTGCATGCGGATTTTGAAAATGTGAAAAAGCGCTTAGAAAGAGACAAGAGCATGGCGTTAGAGTATGCGTAT
GAAAAAATCGCGCTAGATTTATTGCCGGTGATTGATGCACTTCTTGGGGCTCATAAGAGCGCTTCAGGAGATGATAAAGA
GAGCGCTTTAACGAAAGGCTTGGAGCTTACGATGGAAAAGTTGCATGAAGTTTTGGCAAGGCATGGCATTGAGGGGATTG
AATGCTTAGAAGAATTTGATCCCAATTTCCACAATGCGATCATGCAAGTCAAAAGCGAAGAAAAAGAAAATGGGAAAATC
GTGCAAGTTTTGCAACAGGGTTACAAGTATAAAGGTAGGGTTTTAAGGCCTGCAATGGTGAGCATTGCTAAAAACGATTA
A

Upstream 100 bases:

>100_bases
CGCGTCCTATAAAATTTAAAAACATGCAAGTGCAACTGCTTTGTGTAGGGAAATTGGAATGCGATTATGAAGGGTTTTTT
CAAACCATTAAGGAGGAACG

Downstream 100 bases:

>100_bases
ATTTATTTTAAAAAGAAAGGATAACACATGGGAAAAGTTATTGGAATTGATTTAGGGACCACCAACTCTGCAATGGCAGT
GTATGAGGGTAATGAGGCAA

Product: heat shock protein GrpE

Products: NA

Alternate protein names: HSP-70 cofactor [H]

Number of amino acids: Translated: 186; Mature: 186

Protein sequence:

>186_residues
MKDEHNQEHDLSQKELESCENSCTCEGKKQEASEKECEIKEDFELKYQEMHEKYLRVHADFENVKKRLERDKSMALEYAY
EKIALDLLPVIDALLGAHKSASGDDKESALTKGLELTMEKLHEVLARHGIEGIECLEEFDPNFHNAIMQVKSEEKENGKI
VQVLQQGYKYKGRVLRPAMVSIAKND

Sequences:

>Translated_186_residues
MKDEHNQEHDLSQKELESCENSCTCEGKKQEASEKECEIKEDFELKYQEMHEKYLRVHADFENVKKRLERDKSMALEYAY
EKIALDLLPVIDALLGAHKSASGDDKESALTKGLELTMEKLHEVLARHGIEGIECLEEFDPNFHNAIMQVKSEEKENGKI
VQVLQQGYKYKGRVLRPAMVSIAKND
>Mature_186_residues
MKDEHNQEHDLSQKELESCENSCTCEGKKQEASEKECEIKEDFELKYQEMHEKYLRVHADFENVKKRLERDKSMALEYAY
EKIALDLLPVIDALLGAHKSASGDDKESALTKGLELTMEKLHEVLARHGIEGIECLEEFDPNFHNAIMQVKSEEKENGKI
VQVLQQGYKYKGRVLRPAMVSIAKND

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family [H]

Homologues:

Organism=Homo sapiens, GI24308295, Length=161, Percent_Identity=34.1614906832298, Blast_Score=69, Evalue=2e-12,
Organism=Escherichia coli, GI1788967, Length=133, Percent_Identity=39.8496240601504, Blast_Score=98, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI17552458, Length=152, Percent_Identity=31.5789473684211, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6324806, Length=142, Percent_Identity=34.5070422535211, Blast_Score=82, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24653432, Length=171, Percent_Identity=34.5029239766082, Blast_Score=82, Evalue=2e-16,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012 [H]

Pfam domain/function: PF01025 GrpE [H]

EC number: NA

Molecular weight: Translated: 21427; Mature: 21427

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDEHNQEHDLSQKELESCENSCTCEGKKQEASEKECEIKEDFELKYQEMHEKYLRVHAD
CCCCCCCCCCCCHHHHHHHHCCCCCCCCHHHCCHHHCCCHHHHHHHHHHHHHHHHHHHCC
FENVKKRLERDKSMALEYAYEKIALDLLPVIDALLGAHKSASGDDKESALTKGLELTMEK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
LHEVLARHGIEGIECLEEFDPNFHNAIMQVKSEEKENGKIVQVLQQGYKYKGRVLRPAMV
HHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHH
SIAKND
HHHCCC
>Mature Secondary Structure
MKDEHNQEHDLSQKELESCENSCTCEGKKQEASEKECEIKEDFELKYQEMHEKYLRVHAD
CCCCCCCCCCCCHHHHHHHHCCCCCCCCHHHCCHHHCCCHHHHHHHHHHHHHHHHHHHCC
FENVKKRLERDKSMALEYAYEKIALDLLPVIDALLGAHKSASGDDKESALTKGLELTMEK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
LHEVLARHGIEGIECLEEFDPNFHNAIMQVKSEEKENGKIVQVLQQGYKYKGRVLRPAMV
HHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHH
SIAKND
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA