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The map label for this gene is glnA [H]

Identifier: 109947431

GI number: 109947431

Start: 768545

End: 769990

Strand: Reverse

Name: glnA [H]

Synonym: Hac_0878

Alternate gene names: 109947431

Gene position: 769990-768545 (Counterclockwise)

Preceding gene: 109947444

Following gene: 109947427

Centisome position: 49.55

GC content: 40.8

Gene sequence:

>1446_bases
ATGATAACAAGAACTCAAAATACTGAAAGCAAAATCAAAGAATTTTTTGCATTTTGCAAAGAAAATGAAGTGGAATTTGT
GGATTTTAGATTCAGCGATATTAAAGGCACTTGGAATCATATCGCTTATTCTTTTGGGGCTTTAACGCATGACATGTTTA
AAGAGGGGATCCCTTTTGATGCGAGCTCTTTTAAAGGTTGGCAAAGCATTGAACACTCTGATATGATTTTAACCCCTGAT
TTGGTGCGTTACTTCATTGACCCTTTTAGTGCAGATGTGAGCGTGGTCGTGTTTTGCGATGTTTATGATGTGTATAAAAA
CCAGCCTTATGAAAAATGCCCTAGAAGTATCGCTAAAAAAGCCTTAAAGCATTTAAAGGATTTAGGTTTGGGCGATGTGG
CTTATTTTGGTGCGGAGAATGAATTTTTTATCTTTGATTCCATTAAGATTAAAGACGCTTCCAATTCCCAATACTACGAA
GTGGATAGCGAAGAGGGCGAATGGAATAGGGATAGAAGTTTTGAAAATGGCGTGAATTATGGGCATAGACCGGGCAAGCA
AGGGGGCTATTTGCCTGTGCCACCGACGGATACGATGATGGATATTCGCACTGAAATTGTGAAAGTCTTGAATCAAGTGG
GGCTAGAAACTTTTGTTGTCCATCATGAAGTCGCGCAAGCACAAGGTGAAGTGGGCGTGAAATTTGGGGATCTAGTGGAA
GCGGCTGATAATGTCCAAAAACTCAAATATGTGGTTAAAATGGTCGCTCACTTGAACGGCAAAACCGCCACTTTCATGCC
AAAACCTTTGTATGGGGATAATGGGAGTGGGATGCACACCCATGTGAGCATTTGGAAAAACAATGAAAACCTTTTTAGTG
GCGAAACTTACAAGGGCTTGAGCGAGTTGGCGTTGCATTTTTTAGGGGGCGTGTTGCACCACGCTAAAGGGTTAGCCGCT
TTCACTAACGCTTCTACTAATTCTTATAAACGCCTAATTCCAGGCTATGAAGCCCCATCTATTCTAACTTATTCTTCTAA
CAATAGGAGTGCGAGCGTTCGTATTCCTTATGGGATTTCTAAAACTAGCGCGAGGTTTGAATTCAGATTCCCAGATAGCT
CGTCAAACCCCTACTTGGCTTTTGGGGCTATTTTAATGGCAGGTATTGATGGCGTTAAAAATAAGATTGATCCGGGCGAA
GCGATGGATATTAACCTTTTCAAATTGACTTTAGATGAAATCAGAGAAAAGGGCATCAAACAAATGCCCCATACTCTAAG
GAGATCATTAGAAGCGATGTTAGCCGATAGGCAGTATTTAAAAGAGGGGCAAGTCTTTAGCGAAGAATTTATCCAAGCCT
ACCAATCGCTTAAATTCAATACCGAAGTGTTTCCATGGGAGAGCAAACCCCATCCTTTTGAATTTATCACCACTTATTCA
TGTTAA

Upstream 100 bases:

>100_bases
ATCTTTAAGAAAACTTAACGCAAATCTTACTTTTTTACCCCATAAATGCTATAATCACCCCTATGAAATCAAACCAAACT
CGTATAATTGAAGGCCATTA

Downstream 100 bases:

>100_bases
AAATGAATGGGCGGATAAACCTCATTCTAAAAGTTTCAAATAAGGTAAGGCAAAAATCCACTATTTTAAAAAAGAGTGGC
TGAGAAAATTGATTTTTTGC

Product: hypothetical protein

Products: NA

Alternate protein names: Glutamate--ammonia ligase [H]

Number of amino acids: Translated: 481; Mature: 481

Protein sequence:

>481_residues
MITRTQNTESKIKEFFAFCKENEVEFVDFRFSDIKGTWNHIAYSFGALTHDMFKEGIPFDASSFKGWQSIEHSDMILTPD
LVRYFIDPFSADVSVVVFCDVYDVYKNQPYEKCPRSIAKKALKHLKDLGLGDVAYFGAENEFFIFDSIKIKDASNSQYYE
VDSEEGEWNRDRSFENGVNYGHRPGKQGGYLPVPPTDTMMDIRTEIVKVLNQVGLETFVVHHEVAQAQGEVGVKFGDLVE
AADNVQKLKYVVKMVAHLNGKTATFMPKPLYGDNGSGMHTHVSIWKNNENLFSGETYKGLSELALHFLGGVLHHAKGLAA
FTNASTNSYKRLIPGYEAPSILTYSSNNRSASVRIPYGISKTSARFEFRFPDSSSNPYLAFGAILMAGIDGVKNKIDPGE
AMDINLFKLTLDEIREKGIKQMPHTLRRSLEAMLADRQYLKEGQVFSEEFIQAYQSLKFNTEVFPWESKPHPFEFITTYS
C

Sequences:

>Translated_481_residues
MITRTQNTESKIKEFFAFCKENEVEFVDFRFSDIKGTWNHIAYSFGALTHDMFKEGIPFDASSFKGWQSIEHSDMILTPD
LVRYFIDPFSADVSVVVFCDVYDVYKNQPYEKCPRSIAKKALKHLKDLGLGDVAYFGAENEFFIFDSIKIKDASNSQYYE
VDSEEGEWNRDRSFENGVNYGHRPGKQGGYLPVPPTDTMMDIRTEIVKVLNQVGLETFVVHHEVAQAQGEVGVKFGDLVE
AADNVQKLKYVVKMVAHLNGKTATFMPKPLYGDNGSGMHTHVSIWKNNENLFSGETYKGLSELALHFLGGVLHHAKGLAA
FTNASTNSYKRLIPGYEAPSILTYSSNNRSASVRIPYGISKTSARFEFRFPDSSSNPYLAFGAILMAGIDGVKNKIDPGE
AMDINLFKLTLDEIREKGIKQMPHTLRRSLEAMLADRQYLKEGQVFSEEFIQAYQSLKFNTEVFPWESKPHPFEFITTYS
C
>Mature_481_residues
MITRTQNTESKIKEFFAFCKENEVEFVDFRFSDIKGTWNHIAYSFGALTHDMFKEGIPFDASSFKGWQSIEHSDMILTPD
LVRYFIDPFSADVSVVVFCDVYDVYKNQPYEKCPRSIAKKALKHLKDLGLGDVAYFGAENEFFIFDSIKIKDASNSQYYE
VDSEEGEWNRDRSFENGVNYGHRPGKQGGYLPVPPTDTMMDIRTEIVKVLNQVGLETFVVHHEVAQAQGEVGVKFGDLVE
AADNVQKLKYVVKMVAHLNGKTATFMPKPLYGDNGSGMHTHVSIWKNNENLFSGETYKGLSELALHFLGGVLHHAKGLAA
FTNASTNSYKRLIPGYEAPSILTYSSNNRSASVRIPYGISKTSARFEFRFPDSSSNPYLAFGAILMAGIDGVKNKIDPGE
AMDINLFKLTLDEIREKGIKQMPHTLRRSLEAMLADRQYLKEGQVFSEEFIQAYQSLKFNTEVFPWESKPHPFEFITTYS
C

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1790301, Length=467, Percent_Identity=48.8222698072805, Blast_Score=458, Evalue=1e-130,
Organism=Escherichia coli, GI87081870, Length=365, Percent_Identity=25.7534246575342, Blast_Score=100, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24641292, Length=321, Percent_Identity=24.9221183800623, Blast_Score=73, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24641290, Length=321, Percent_Identity=24.9221183800623, Blast_Score=73, Evalue=5e-13,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809 [H]

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 54476; Mature: 54476

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS00181 GLNA_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITRTQNTESKIKEFFAFCKENEVEFVDFRFSDIKGTWNHIAYSFGALTHDMFKEGIPFD
CCCCCCCHHHHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCC
ASSFKGWQSIEHSDMILTPDLVRYFIDPFSADVSVVVFCDVYDVYKNQPYEKCPRSIAKK
CCCCCCHHCCCCCCEEECHHHHHHHHCCCCCCCEEEEEEEHHHHHCCCCHHHHHHHHHHH
ALKHLKDLGLGDVAYFGAENEFFIFDSIKIKDASNSQYYEVDSEEGEWNRDRSFENGVNY
HHHHHHHCCCCCEEEECCCCCEEEEEEEEEECCCCCCEEEECCCCCCCCCCCCHHHCCCC
GHRPGKQGGYLPVPPTDTMMDIRTEIVKVLNQVGLETFVVHHEVAQAQGEVGVKFGDLVE
CCCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCCHHHHHH
AADNVQKLKYVVKMVAHLNGKTATFMPKPLYGDNGSGMHTHVSIWKNNENLFSGETYKGL
HHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCEEEEEEEECCCCCCCCCHHCCH
SELALHFLGGVLHHAKGLAAFTNASTNSYKRLIPGYEAPSILTYSSNNRSASVRIPYGIS
HHHHHHHHHHHHHHHCCHHEEECCCCCCHHHHCCCCCCCEEEEEECCCCEEEEEECCCCC
KTSARFEFRFPDSSSNPYLAFGAILMAGIDGVKNKIDPGEAMDINLFKLTLDEIREKGIK
CCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEHHHHHHHHHHHH
QMPHTLRRSLEAMLADRQYLKEGQVFSEEFIQAYQSLKFNTEVFPWESKPHPFEFITTYS
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCEEECCCCCCCCEEEEEECC
C
C
>Mature Secondary Structure
MITRTQNTESKIKEFFAFCKENEVEFVDFRFSDIKGTWNHIAYSFGALTHDMFKEGIPFD
CCCCCCCHHHHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCC
ASSFKGWQSIEHSDMILTPDLVRYFIDPFSADVSVVVFCDVYDVYKNQPYEKCPRSIAKK
CCCCCCHHCCCCCCEEECHHHHHHHHCCCCCCCEEEEEEEHHHHHCCCCHHHHHHHHHHH
ALKHLKDLGLGDVAYFGAENEFFIFDSIKIKDASNSQYYEVDSEEGEWNRDRSFENGVNY
HHHHHHHCCCCCEEEECCCCCEEEEEEEEEECCCCCCEEEECCCCCCCCCCCCHHHCCCC
GHRPGKQGGYLPVPPTDTMMDIRTEIVKVLNQVGLETFVVHHEVAQAQGEVGVKFGDLVE
CCCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCCHHHHHH
AADNVQKLKYVVKMVAHLNGKTATFMPKPLYGDNGSGMHTHVSIWKNNENLFSGETYKGL
HHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCEEEEEEEECCCCCCCCCHHCCH
SELALHFLGGVLHHAKGLAAFTNASTNSYKRLIPGYEAPSILTYSSNNRSASVRIPYGIS
HHHHHHHHHHHHHHHCCHHEEECCCCCCHHHHCCCCCCCEEEEEECCCCEEEEEECCCCC
KTSARFEFRFPDSSSNPYLAFGAILMAGIDGVKNKIDPGEAMDINLFKLTLDEIREKGIK
CCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEHHHHHHHHHHHH
QMPHTLRRSLEAMLADRQYLKEGQVFSEEFIQAYQSLKFNTEVFPWESKPHPFEFITTYS
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCEEECCCCCCCCEEEEEECC
C
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]