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The map label for this gene is trmB

Identifier: 109947246

GI number: 109947246

Start: 587207

End: 588403

Strand: Reverse

Name: trmB

Synonym: Hac_0671

Alternate gene names: 109947246

Gene position: 588403-587207 (Counterclockwise)

Preceding gene: 109947247

Following gene: 109947245

Centisome position: 37.87

GC content: 33.08

Gene sequence:

>1197_bases
ATGCCCCATTTTTTAGCCAAGTTAGATTGCAAACCTTTAGAATACCCAATAATAAGTGGGGATTTTTGTTTTCATAAAGA
ATTTTCAAGCTTAAAACACCCCACTAAAAGTTGTGTGTATGCGAGTTTTAAAGACCATATTTTTTTATTGCAAAAAATCA
GGCGAGCGAATGATTTTTTAATCAAAAGCGAAAAAGCAACGCCCTTAAAAAGAGAGATTTTAAAACAAGCCTTAAGGATT
TATGCACAATCTTTTGAAGTGATTTTGCATAATTTGCAAGAAAATTCTAAACATGCGAGCGGAAAAAAAGCCCTTGATTT
AGAGGATTTTGAAGACTTTATCAAAGAAAATCAAGCCCCTATTTTAATGGAAATTGGTTTTGGGAGTGGGAGGCATTTGA
TGGAATTAGCCAAAAACAACCCCACTAAAACATGTTTAGGGATAGAAATTTATACCCCCTCTATCGCACAAGTATTAAAA
CAAATAGAATTATTGGATTTAAAAAATCTGCATGTGTTACAAGGCGATGGTCGTTTGGTTTTAGAGAGCATACCGCATCA
CAAGTGCGAGAAAATTTTTGTGCATTTCCCTGTGCCGTGGAATGACAAAAAACACCGCCGAGTGTTAAGCGAAAGATTTT
TAGACGAAGCTTTAAGGGTTTTAGAACCTAATGGGTTTTTGGAATTACGCACTGATGATACTCTTTATTTTGAAGACAGC
CTAAAATTAGCCTTAAAACATTTTAAAAGCGAAATAGAAATCAAAAAAAACGCTCAAATCCCAGTTGTCAGCAAGTATGA
AGCGCGTTGGAATAAACTCAAAAAAGATATTTATGATTTAAGAATTTATTCTTTAGAATCCAATGAAACCCCTTTTAATA
ACCATGCATTTGATTTTTCTTTTGATACAATAACACTTAATCAAAAGAGCGTTGGAATGATTTTAAAAACCCCAAAAATC
ATTAAAGAAGGGTATTTTGTGCATGTTTGCAATATCTATGAGAATAAGGGGGATTTTTTAGTGGAATTGAGCATGGGGGA
TTTTGATTGGCCTATGCGTTTGTTTGTTTTATCAGTAAAAAATCAAGTTTTTTACCTCAATAAAAGCCCATTAAAAACTT
TAAACAACCACAAAGCGCATCTTTTGCTTCAAAATATCCTAAAGGAATTTGATGAGTATAATCATTGCAGCGAATAA

Upstream 100 bases:

>100_bases
GTGCCTTATCGCTATCAGGTAGTGAGCGTGGATAAAGATGGTTTAGAATCGCACCCTAGCAAAGAAGTGCGTTTGTTTTT
AGAGCGCTAAAAGAGTTTGA

Downstream 100 bases:

>100_bases
TCTATGCTTACAATACCAACAAAACGAGCCGGTCATCAAACATGCTAATTTACGCATCAAACGGAAGGATTTTGTGTTTA
TTTCAGGGTCTAGTGGGAGC

Product: tRNA (guanine-N(7)-)-methyltransferase

Products: NA

Alternate protein names: tRNA(m7G46)-methyltransferase

Number of amino acids: Translated: 398; Mature: 397

Protein sequence:

>398_residues
MPHFLAKLDCKPLEYPIISGDFCFHKEFSSLKHPTKSCVYASFKDHIFLLQKIRRANDFLIKSEKATPLKREILKQALRI
YAQSFEVILHNLQENSKHASGKKALDLEDFEDFIKENQAPILMEIGFGSGRHLMELAKNNPTKTCLGIEIYTPSIAQVLK
QIELLDLKNLHVLQGDGRLVLESIPHHKCEKIFVHFPVPWNDKKHRRVLSERFLDEALRVLEPNGFLELRTDDTLYFEDS
LKLALKHFKSEIEIKKNAQIPVVSKYEARWNKLKKDIYDLRIYSLESNETPFNNHAFDFSFDTITLNQKSVGMILKTPKI
IKEGYFVHVCNIYENKGDFLVELSMGDFDWPMRLFVLSVKNQVFYLNKSPLKTLNNHKAHLLLQNILKEFDEYNHCSE

Sequences:

>Translated_398_residues
MPHFLAKLDCKPLEYPIISGDFCFHKEFSSLKHPTKSCVYASFKDHIFLLQKIRRANDFLIKSEKATPLKREILKQALRI
YAQSFEVILHNLQENSKHASGKKALDLEDFEDFIKENQAPILMEIGFGSGRHLMELAKNNPTKTCLGIEIYTPSIAQVLK
QIELLDLKNLHVLQGDGRLVLESIPHHKCEKIFVHFPVPWNDKKHRRVLSERFLDEALRVLEPNGFLELRTDDTLYFEDS
LKLALKHFKSEIEIKKNAQIPVVSKYEARWNKLKKDIYDLRIYSLESNETPFNNHAFDFSFDTITLNQKSVGMILKTPKI
IKEGYFVHVCNIYENKGDFLVELSMGDFDWPMRLFVLSVKNQVFYLNKSPLKTLNNHKAHLLLQNILKEFDEYNHCSE
>Mature_397_residues
PHFLAKLDCKPLEYPIISGDFCFHKEFSSLKHPTKSCVYASFKDHIFLLQKIRRANDFLIKSEKATPLKREILKQALRIY
AQSFEVILHNLQENSKHASGKKALDLEDFEDFIKENQAPILMEIGFGSGRHLMELAKNNPTKTCLGIEIYTPSIAQVLKQ
IELLDLKNLHVLQGDGRLVLESIPHHKCEKIFVHFPVPWNDKKHRRVLSERFLDEALRVLEPNGFLELRTDDTLYFEDSL
KLALKHFKSEIEIKKNAQIPVVSKYEARWNKLKKDIYDLRIYSLESNETPFNNHAFDFSFDTITLNQKSVGMILKTPKII
KEGYFVHVCNIYENKGDFLVELSMGDFDWPMRLFVLSVKNQVFYLNKSPLKTLNNHKAHLLLQNILKEFDEYNHCSE

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA

COG id: COG0220

COG function: function code R; Predicted S-adenosylmethionine-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmB family

Homologues:

Organism=Escherichia coli, GI1789330, Length=184, Percent_Identity=30.9782608695652, Blast_Score=81, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMB_HELAH (Q17Y01)

Other databases:

- EMBL:   AM260522
- RefSeq:   YP_664474.1
- ProteinModelPortal:   Q17Y01
- SMR:   Q17Y01
- STRING:   Q17Y01
- GeneID:   4176664
- GenomeReviews:   AM260522_GR
- KEGG:   hac:Hac_0671
- NMPDR:   fig|382638.8.peg.653
- eggNOG:   COG0220
- HOGENOM:   HBG321449
- OMA:   FVHFPVP
- PhylomeDB:   Q17Y01
- ProtClustDB:   PRK14121
- BioCyc:   HACI382638:HAC_0671-MONOMER
- HAMAP:   MF_01057
- InterPro:   IPR003358
- PANTHER:   PTHR23417:SF1
- TIGRFAMs:   TIGR00091

Pfam domain/function: PF02390 Methyltransf_4

EC number: =2.1.1.33

Molecular weight: Translated: 46494; Mature: 46363

Theoretical pI: Translated: 8.29; Mature: 8.29

Prosite motif: NA

Important sites: BINDING 124-124 BINDING 149-149 BINDING 176-176 BINDING 232-232

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPHFLAKLDCKPLEYPIISGDFCFHKEFSSLKHPTKSCVYASFKDHIFLLQKIRRANDFL
CCCCCCCCCCCCCCCCEECCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCEE
IKSEKATPLKREILKQALRIYAQSFEVILHNLQENSKHASGKKALDLEDFEDFIKENQAP
EECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCC
ILMEIGFGSGRHLMELAKNNPTKTCLGIEIYTPSIAQVLKQIELLDLKNLHVLQGDGRLV
EEEEECCCCCHHHHHHHHCCCCCEEEEEEEECCHHHHHHHHHHHHCCCCEEEEECCCEEE
LESIPHHKCEKIFVHFPVPWNDKKHRRVLSERFLDEALRVLEPNGFLELRTDDTLYFEDS
EECCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCEEEEHHH
LKLALKHFKSEIEIKKNAQIPVVSKYEARWNKLKKDIYDLRIYSLESNETPFNNHAFDFS
HHHHHHHHHHHEEEECCCCCCEEEHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCEEEEE
FDTITLNQKSVGMILKTPKIIKEGYFVHVCNIYENKGDFLVELSMGDFDWPMRLFVLSVK
EEEEEECCCCCCEEECCCHHHHCCCEEEEEEEEECCCCEEEEEECCCCCCCHHHEEEEEC
NQVFYLNKSPLKTLNNHKAHLLLQNILKEFDEYNHCSE
CEEEEECCCCHHHHCCCHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
PHFLAKLDCKPLEYPIISGDFCFHKEFSSLKHPTKSCVYASFKDHIFLLQKIRRANDFL
CCCCCCCCCCCCCCCEECCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCEE
IKSEKATPLKREILKQALRIYAQSFEVILHNLQENSKHASGKKALDLEDFEDFIKENQAP
EECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCC
ILMEIGFGSGRHLMELAKNNPTKTCLGIEIYTPSIAQVLKQIELLDLKNLHVLQGDGRLV
EEEEECCCCCHHHHHHHHCCCCCEEEEEEEECCHHHHHHHHHHHHCCCCEEEEECCCEEE
LESIPHHKCEKIFVHFPVPWNDKKHRRVLSERFLDEALRVLEPNGFLELRTDDTLYFEDS
EECCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCEEEEHHH
LKLALKHFKSEIEIKKNAQIPVVSKYEARWNKLKKDIYDLRIYSLESNETPFNNHAFDFS
HHHHHHHHHHHEEEECCCCCCEEEHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCEEEEE
FDTITLNQKSVGMILKTPKIIKEGYFVHVCNIYENKGDFLVELSMGDFDWPMRLFVLSVK
EEEEEECCCCCCEEECCCHHHHCCCEEEEEEEEECCCCEEEEEECCCCCCCHHHEEEEEC
NQVFYLNKSPLKTLNNHKAHLLLQNILKEFDEYNHCSE
CEEEEECCCCHHHHCCCHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA