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The map label for this gene is purB [H]

Identifier: 109947032

GI number: 109947032

Start: 373021

End: 374343

Strand: Reverse

Name: purB [H]

Synonym: Hac_0429

Alternate gene names: 109947032

Gene position: 374343-373021 (Counterclockwise)

Preceding gene: 109947033

Following gene: 109947031

Centisome position: 24.09

GC content: 40.06

Gene sequence:

>1323_bases
GTGTTAGAACGCTATGCGAATGAAGAAATGAAAGCCTTATGGAGCGAGCGAACCAAGTTTGAAACCTATTTAGAAGTGGA
AAAAGCTGTGGTTAGGGCATGGAATAAGCTTGGACAAATCAATGATAGCGATTGCGAAAAAATCTGCTCTCAAGCGAAGT
TTAACCTGAAGCGCATCAAAGAAATTGAAAAGACCACTAAACATGATTTAATCGCTTTCACTATGTGCGTGGCTGAAAGC
TTGGGCGAAGAGTCTCGCTTTTTTCATTATGGCATCACTTCTAGTGATTGCATTGACACAGCTATGGCATTATTGATGAC
AAAAAGTTTAAAGCTCATTCAAAAAAGCGTTAAAAACCTCTATGAAACACTTAAAAATAGGGCTTTAGAGCATAAAGACA
CGCTAATGGTAGGCAGAAGCCATGGGGTGTTTGGCGAACCCATCACTTTTGGTTTAGTTTTAGCCCTTTTTGCTGATGAA
ATCAAACGGCATTTAAAAGCCTTGGATTTGACGATGGAGTTTATTAGCGTGGGGGCGATTAGTGGAGCTATGGGGAATTT
CGCGCACGCTCCCTTAGAATTAGAAGAATTAGCGTGCGGATTTTTAGGTTTAAAAACAGCCAGTATCAGCAATCAAGTCA
TTCAAAGAGATCGCTACGCTAGGCTTGCATGCGATTTGGCTCTTTTAGCGAGCAGTTGTGAAAAAATCGCTATCAATATC
CGCCATTTGCAACGCAGTGAAGTCTATGAAGTGGAAGAATACTTTTCAATAGGGCAAAAGGGAAGCTCTGCGATGCCCCA
TAAAAGAAACCCCATATTGAGCGAAAATATCACTGGGCTTTGTAGGGTGATTCGCTCTTTTACGACCCCTATGCTAGAAA
ATGTTGCCCTATGGCATGAAAGAGACATGAGCCATAGCTCTGTGGAGCGTTTTGCCTTACCCGATCTATTTATCACTAGC
GATTTTATGCTCAGCCGCTTGAATGGCGTGGTTGAAAATTTAGTGGTCTATCCCAAGAACATGCTTAAAAATTTAGCCTT
GAGTGGGGGGTTAGTCTTTTCGCAACGAGTATTATTAGAATTGCCTAAAAAAGGTTTGAGCAGAGAAGAAAGCTATAAAA
TCGTGCAAGAAAATGCGATGAAAATATGGGAAGTTTTGCAACAAGGCACCTTTAAAAACGCTGATGAAAATTTGTTTTTG
AACGCCCTGCTTGATGATGAACGCTTGAAAAAATATTTGAGCAAGGATGAAATCAAAGCGTGTTTTGATTATAGCTATTA
CACTAAAAATGTAGATGCGATTTTTAAAAGGGTGTTTGAATAA

Upstream 100 bases:

>100_bases
AAGTATAACTCTTCAATCAAATCAAGGGGCTTTTTGGGTGCATCTTTTATCCCCTTGATTTTACCCCTTTCGAATAAATT
CTAGATTTTAAGGATTGTTG

Downstream 100 bases:

>100_bases
GGCGCGATTTTACAAAAAATTTTGGCTTTATTCATTGAGTGATAAAGTTTAAAATAATAAAATAAAGTTTTCATTTTTTA
AAAAATTAAAATTTCACTCA

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase [H]

Number of amino acids: Translated: 440; Mature: 440

Protein sequence:

>440_residues
MLERYANEEMKALWSERTKFETYLEVEKAVVRAWNKLGQINDSDCEKICSQAKFNLKRIKEIEKTTKHDLIAFTMCVAES
LGEESRFFHYGITSSDCIDTAMALLMTKSLKLIQKSVKNLYETLKNRALEHKDTLMVGRSHGVFGEPITFGLVLALFADE
IKRHLKALDLTMEFISVGAISGAMGNFAHAPLELEELACGFLGLKTASISNQVIQRDRYARLACDLALLASSCEKIAINI
RHLQRSEVYEVEEYFSIGQKGSSAMPHKRNPILSENITGLCRVIRSFTTPMLENVALWHERDMSHSSVERFALPDLFITS
DFMLSRLNGVVENLVVYPKNMLKNLALSGGLVFSQRVLLELPKKGLSREESYKIVQENAMKIWEVLQQGTFKNADENLFL
NALLDDERLKKYLSKDEIKACFDYSYYTKNVDAIFKRVFE

Sequences:

>Translated_440_residues
MLERYANEEMKALWSERTKFETYLEVEKAVVRAWNKLGQINDSDCEKICSQAKFNLKRIKEIEKTTKHDLIAFTMCVAES
LGEESRFFHYGITSSDCIDTAMALLMTKSLKLIQKSVKNLYETLKNRALEHKDTLMVGRSHGVFGEPITFGLVLALFADE
IKRHLKALDLTMEFISVGAISGAMGNFAHAPLELEELACGFLGLKTASISNQVIQRDRYARLACDLALLASSCEKIAINI
RHLQRSEVYEVEEYFSIGQKGSSAMPHKRNPILSENITGLCRVIRSFTTPMLENVALWHERDMSHSSVERFALPDLFITS
DFMLSRLNGVVENLVVYPKNMLKNLALSGGLVFSQRVLLELPKKGLSREESYKIVQENAMKIWEVLQQGTFKNADENLFL
NALLDDERLKKYLSKDEIKACFDYSYYTKNVDAIFKRVFE
>Mature_440_residues
MLERYANEEMKALWSERTKFETYLEVEKAVVRAWNKLGQINDSDCEKICSQAKFNLKRIKEIEKTTKHDLIAFTMCVAES
LGEESRFFHYGITSSDCIDTAMALLMTKSLKLIQKSVKNLYETLKNRALEHKDTLMVGRSHGVFGEPITFGLVLALFADE
IKRHLKALDLTMEFISVGAISGAMGNFAHAPLELEELACGFLGLKTASISNQVIQRDRYARLACDLALLASSCEKIAINI
RHLQRSEVYEVEEYFSIGQKGSSAMPHKRNPILSENITGLCRVIRSFTTPMLENVALWHERDMSHSSVERFALPDLFITS
DFMLSRLNGVVENLVVYPKNMLKNLALSGGLVFSQRVLLELPKKGLSREESYKIVQENAMKIWEVLQQGTFKNADENLFL
NALLDDERLKKYLSKDEIKACFDYSYYTKNVDAIFKRVFE

Specific function: De novo purine biosynthesis; eighth step. [C]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557269, Length=453, Percent_Identity=25.3863134657837, Blast_Score=125, Evalue=7e-29,
Organism=Homo sapiens, GI183227688, Length=402, Percent_Identity=27.1144278606965, Blast_Score=124, Evalue=2e-28,
Organism=Escherichia coli, GI1787376, Length=289, Percent_Identity=27.681660899654, Blast_Score=82, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17508577, Length=342, Percent_Identity=26.3157894736842, Blast_Score=104, Evalue=8e-23,
Organism=Caenorhabditis elegans, GI32564234, Length=307, Percent_Identity=27.6872964169381, Blast_Score=88, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6323391, Length=390, Percent_Identity=26.4102564102564, Blast_Score=133, Evalue=5e-32,
Organism=Drosophila melanogaster, GI24647570, Length=380, Percent_Identity=26.3157894736842, Blast_Score=115, Evalue=5e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 50197; Mature: 50197

Theoretical pI: Translated: 7.25; Mature: 7.25

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLERYANEEMKALWSERTKFETYLEVEKAVVRAWNKLGQINDSDCEKICSQAKFNLKRIK
CCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
EIEKTTKHDLIAFTMCVAESLGEESRFFHYGITSSDCIDTAMALLMTKSLKLIQKSVKNL
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
YETLKNRALEHKDTLMVGRSHGVFGEPITFGLVLALFADEIKRHLKALDLTMEFISVGAI
HHHHHHHHHHHHHHEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SGAMGNFAHAPLELEELACGFLGLKTASISNQVIQRDRYARLACDLALLASSCEKIAINI
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RHLQRSEVYEVEEYFSIGQKGSSAMPHKRNPILSENITGLCRVIRSFTTPMLENVALWHE
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
RDMSHSSVERFALPDLFITSDFMLSRLNGVVENLVVYPKNMLKNLALSGGLVFSQRVLLE
CCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHH
LPKKGLSREESYKIVQENAMKIWEVLQQGTFKNADENLFLNALLDDERLKKYLSKDEIKA
HCHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEHHHHCCHHHHHHHHCHHHHHH
CFDYSYYTKNVDAIFKRVFE
HHHHHHHHCCHHHHHHHHCC
>Mature Secondary Structure
MLERYANEEMKALWSERTKFETYLEVEKAVVRAWNKLGQINDSDCEKICSQAKFNLKRIK
CCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
EIEKTTKHDLIAFTMCVAESLGEESRFFHYGITSSDCIDTAMALLMTKSLKLIQKSVKNL
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
YETLKNRALEHKDTLMVGRSHGVFGEPITFGLVLALFADEIKRHLKALDLTMEFISVGAI
HHHHHHHHHHHHHHEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SGAMGNFAHAPLELEELACGFLGLKTASISNQVIQRDRYARLACDLALLASSCEKIAINI
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RHLQRSEVYEVEEYFSIGQKGSSAMPHKRNPILSENITGLCRVIRSFTTPMLENVALWHE
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
RDMSHSSVERFALPDLFITSDFMLSRLNGVVENLVVYPKNMLKNLALSGGLVFSQRVLLE
CCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHH
LPKKGLSREESYKIVQENAMKIWEVLQQGTFKNADENLFLNALLDDERLKKYLSKDEIKA
HCHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEHHHHCCHHHHHHHHCHHHHHH
CFDYSYYTKNVDAIFKRVFE
HHHHHHHHCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]