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The map label for this gene is hemB [H]

Identifier: 109946955

GI number: 109946955

Start: 292842

End: 293813

Strand: Reverse

Name: hemB [H]

Synonym: Hac_0345

Alternate gene names: 109946955

Gene position: 293813-292842 (Counterclockwise)

Preceding gene: 109946956

Following gene: 109946954

Centisome position: 18.91

GC content: 39.51

Gene sequence:

>972_bases
ATGTTCAAACGATTGAGAAGACTACGAAGTAGCGAAAATTTAAGGGCTATGATAAGAGAAACGCGTTTAGATATTAACGA
TTTCATCGCCCCCCTATTTGTCATAGAAAGCGATGGTAATATTAAAAATGAAATTAGCTCCATGCCTAGGGTTTATCAAA
TGAGTATCGAGCCTCTTTTGAAAGAATGCGAAGAGTTGGTGAGTCTTGGTATCAAAGCCGTTCTATTGTTTGGTATCCCT
AAACACAAGGACGCCACAGGAAGCCATGCGTTAAATAAGGATCATATTGCCGCAAGAGCCACGAGAGAGATTAAAAAACG
ATTTAAGGATTTGATCGTTATAGCGGATTTGTGTTTTTGTGAATACACTGACCATGGGCATTGCGGGATTTTAGAAAACA
CTTCTGTGTCTAACGATAAAACGCTAGAGATTTTAAACCTTCAAGGGCTTATTTTAGCCGAAAGCGGTGTGGATATTTTA
GCCCCAAGCAGCATGATGGATGGGAATGTTTTAAGCTTAAGAAACGCACTGGATAAGGCTGGGTATTTTTGCACGCCTAT
TATGAGCTATTCCACTAAATTTGCGAGCAGCTATTATGGGCCTTTTAGAGATGCGGCAAATTCCGCACCAAGTTTTGGCG
ATCGGAAGAGCTATCAAATGGATTACGGCAATCAAAAAGAAGCGCTTTTAGAAAGTTTAGAAGATGAAAAACAGGGTGCG
GATATTTTAATGGTCAAACCGGCTTTAGCGTATTTGGATATTGTTAAAGAGATTAGAAATCACACTTTACTCCCTTTAGC
GCTCTATAATGTGAGTGGGGAATACGCCATGCTCAAACTCGCTCAAAAACACAACTTGATCAATTATGAAAGCGTGCTGT
TAGAAACGATGACTTGCTTTAAAAGAGCGGGAGCGGATATGATTATTAGCTATCATGCTAAAGAAGTGGCTAACTTATTA
CAAAGGAATTGA

Upstream 100 bases:

>100_bases
GCTACCCCCCCCTCCGAAAATTTGAAAGAAATAAAAGAGATGAAAGAGACAAAAAAGACCGATTGCAAAGCTAAAGAAAG
CCAATGTATAGAGGGAAAAC

Downstream 100 bases:

>100_bases
ATGGGACGAGCGTTTGAATACAGAAGAGCGGCTAAAGAAAAACGATGGGATAAGATGAGTAAGGTTTTCCCCAAACTCGC
AAAAGCGATCACTCTAGCGG

Product: delta-aminolevulinic acid dehydratase

Products: NA

Alternate protein names: ALAD; ALADH; Porphobilinogen synthase [H]

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
MFKRLRRLRSSENLRAMIRETRLDINDFIAPLFVIESDGNIKNEISSMPRVYQMSIEPLLKECEELVSLGIKAVLLFGIP
KHKDATGSHALNKDHIAARATREIKKRFKDLIVIADLCFCEYTDHGHCGILENTSVSNDKTLEILNLQGLILAESGVDIL
APSSMMDGNVLSLRNALDKAGYFCTPIMSYSTKFASSYYGPFRDAANSAPSFGDRKSYQMDYGNQKEALLESLEDEKQGA
DILMVKPALAYLDIVKEIRNHTLLPLALYNVSGEYAMLKLAQKHNLINYESVLLETMTCFKRAGADMIISYHAKEVANLL
QRN

Sequences:

>Translated_323_residues
MFKRLRRLRSSENLRAMIRETRLDINDFIAPLFVIESDGNIKNEISSMPRVYQMSIEPLLKECEELVSLGIKAVLLFGIP
KHKDATGSHALNKDHIAARATREIKKRFKDLIVIADLCFCEYTDHGHCGILENTSVSNDKTLEILNLQGLILAESGVDIL
APSSMMDGNVLSLRNALDKAGYFCTPIMSYSTKFASSYYGPFRDAANSAPSFGDRKSYQMDYGNQKEALLESLEDEKQGA
DILMVKPALAYLDIVKEIRNHTLLPLALYNVSGEYAMLKLAQKHNLINYESVLLETMTCFKRAGADMIISYHAKEVANLL
QRN
>Mature_323_residues
MFKRLRRLRSSENLRAMIRETRLDINDFIAPLFVIESDGNIKNEISSMPRVYQMSIEPLLKECEELVSLGIKAVLLFGIP
KHKDATGSHALNKDHIAARATREIKKRFKDLIVIADLCFCEYTDHGHCGILENTSVSNDKTLEILNLQGLILAESGVDIL
APSSMMDGNVLSLRNALDKAGYFCTPIMSYSTKFASSYYGPFRDAANSAPSFGDRKSYQMDYGNQKEALLESLEDEKQGA
DILMVKPALAYLDIVKEIRNHTLLPLALYNVSGEYAMLKLAQKHNLINYESVLLETMTCFKRAGADMIISYHAKEVANLL
QRN

Specific function: Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen [H]

COG id: COG0113

COG function: function code H; Delta-aminolevulinic acid dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ALADH family [H]

Homologues:

Organism=Homo sapiens, GI189083849, Length=306, Percent_Identity=41.8300653594771, Blast_Score=243, Evalue=1e-64,
Organism=Escherichia coli, GI87081728, Length=318, Percent_Identity=52.2012578616352, Blast_Score=317, Evalue=6e-88,
Organism=Saccharomyces cerevisiae, GI6321398, Length=313, Percent_Identity=36.1022364217252, Blast_Score=197, Evalue=2e-51,
Organism=Drosophila melanogaster, GI21358291, Length=303, Percent_Identity=36.963696369637, Blast_Score=219, Evalue=3e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001731
- InterPro:   IPR013785 [H]

Pfam domain/function: PF00490 ALAD [H]

EC number: =4.2.1.24 [H]

Molecular weight: Translated: 36309; Mature: 36309

Theoretical pI: Translated: 6.94; Mature: 6.94

Prosite motif: PS00169 D_ALA_DEHYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKRLRRLRSSENLRAMIRETRLDINDFIAPLFVIESDGNIKNEISSMPRVYQMSIEPLL
CHHHHHHHHCCCHHHHHHHHHHCCHHHHHCEEEEEECCCCHHHHHHHCCHHHHHHHHHHH
KECEELVSLGIKAVLLFGIPKHKDATGSHALNKDHIAARATREIKKRFKDLIVIADLCFC
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EYTDHGHCGILENTSVSNDKTLEILNLQGLILAESGVDILAPSSMMDGNVLSLRNALDKA
CCCCCCCCCEECCCCCCCCCEEEEEECCEEEEECCCCCEECCCCCCCCCHHHHHHHHHHC
GYFCTPIMSYSTKFASSYYGPFRDAANSAPSFGDRKSYQMDYGNQKEALLESLEDEKQGA
CCCHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCC
DILMVKPALAYLDIVKEIRNHTLLPLALYNVSGEYAMLKLAQKHNLINYESVLLETMTCF
CEEEECHHHHHHHHHHHHHCCCEEEEEEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHH
KRAGADMIISYHAKEVANLLQRN
HHCCCHHEEHHHHHHHHHHHHCC
>Mature Secondary Structure
MFKRLRRLRSSENLRAMIRETRLDINDFIAPLFVIESDGNIKNEISSMPRVYQMSIEPLL
CHHHHHHHHCCCHHHHHHHHHHCCHHHHHCEEEEEECCCCHHHHHHHCCHHHHHHHHHHH
KECEELVSLGIKAVLLFGIPKHKDATGSHALNKDHIAARATREIKKRFKDLIVIADLCFC
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EYTDHGHCGILENTSVSNDKTLEILNLQGLILAESGVDILAPSSMMDGNVLSLRNALDKA
CCCCCCCCCEECCCCCCCCCEEEEEECCEEEEECCCCCEECCCCCCCCCHHHHHHHHHHC
GYFCTPIMSYSTKFASSYYGPFRDAANSAPSFGDRKSYQMDYGNQKEALLESLEDEKQGA
CCCHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCC
DILMVKPALAYLDIVKEIRNHTLLPLALYNVSGEYAMLKLAQKHNLINYESVLLETMTCF
CEEEECHHHHHHHHHHHHHCCCEEEEEEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHH
KRAGADMIISYHAKEVANLLQRN
HHCCCHHEEHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]