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The map label for this gene is nlaIIIR [H]

Identifier: 109946918

GI number: 109946918

Start: 259057

End: 259575

Strand: Direct

Name: nlaIIIR [H]

Synonym: Hac_0305

Alternate gene names: 109946918

Gene position: 259057-259575 (Clockwise)

Preceding gene: 109946917

Following gene: 109946919

Centisome position: 16.67

GC content: 36.22

Gene sequence:

>519_bases
TTGGAGTTTGATAAGGAGCAAACTCCAAGAAATTCTATTGATAGAATACGCTTGAATGGCTATAATACCAAGTGTGTTTT
TAACCAAAGTATCCGTCAAGATATTAAAAATCACTATAGCCAACAATGTTGTGCTATGTGCGGTGTGTGGGGCAACTCTG
AAAACACTCAAATAGAGGTAGATCACAAAGACGGCCGCAAAAATGATCCAAGAGTTTCTGATTTAAGCATGCAGACTTTA
GATGATTTTCAGGCTTTATGTAAAGCTTGTAATGATAAGAAACGCCAAATTTGTAAAGAATGCAAAGAGACTGGCTATAG
ATTTGATGCAACAAAAATTCTTGGCAATCATTATCCTTTCTATGAGGGGGAGGCTGAATATGACGGCTGTGTGGGTTGCT
ATCAATATGACCCCATACAATACAAGAAAACTTGTAATGATAGGATATACAATGAAGGGTATCAAAAAGGTTATGATGAG
GGGTATCAAATTGGATACCATCAAAAAACTACTTTATAG

Upstream 100 bases:

>100_bases
GGGTAAGCGTTACAGAATTTGTAGGAAAATATCAAGAATTACAGCTAGGTAATGGGGGAGTTGGTGTAGGAAGAGTTCAT
CTATAGCTAAAGAATTTAAC

Downstream 100 bases:

>100_bases
TGGTTGCAATGAACTACATCGGTTCTAAATACAAGCTCATTCCCTTTATTAAGGAAAATATCCATGCGGTTGTGGGCAGT
GATCTCTCTTGCACGATTTT

Product: ulcer-associated gene restriction endonuclease

Products: NA

Alternate protein names: R.NlaIII; Endonuclease NlaIII; Type II restriction enzyme NlaIII [H]

Number of amino acids: Translated: 172; Mature: 172

Protein sequence:

>172_residues
MEFDKEQTPRNSIDRIRLNGYNTKCVFNQSIRQDIKNHYSQQCCAMCGVWGNSENTQIEVDHKDGRKNDPRVSDLSMQTL
DDFQALCKACNDKKRQICKECKETGYRFDATKILGNHYPFYEGEAEYDGCVGCYQYDPIQYKKTCNDRIYNEGYQKGYDE
GYQIGYHQKTTL

Sequences:

>Translated_172_residues
MEFDKEQTPRNSIDRIRLNGYNTKCVFNQSIRQDIKNHYSQQCCAMCGVWGNSENTQIEVDHKDGRKNDPRVSDLSMQTL
DDFQALCKACNDKKRQICKECKETGYRFDATKILGNHYPFYEGEAEYDGCVGCYQYDPIQYKKTCNDRIYNEGYQKGYDE
GYQIGYHQKTTL
>Mature_172_residues
MEFDKEQTPRNSIDRIRLNGYNTKCVFNQSIRQDIKNHYSQQCCAMCGVWGNSENTQIEVDHKDGRKNDPRVSDLSMQTL
DDFQALCKACNDKKRQICKECKETGYRFDATKILGNHYPFYEGEAEYDGCVGCYQYDPIQYKKTCNDRIYNEGYQKGYDE
GYQIGYHQKTTL

Specific function: Recognizes the double-stranded sequence CATG and cleaves after G-4 [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003615
- InterPro:   IPR007979 [H]

Pfam domain/function: PF05315 ICEA [H]

EC number: =3.1.21.4 [H]

Molecular weight: Translated: 20164; Mature: 20164

Theoretical pI: Translated: 6.49; Mature: 6.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

6.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
8.1 %Cys+Met (Translated Protein)
6.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
8.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEFDKEQTPRNSIDRIRLNGYNTKCVFNQSIRQDIKNHYSQQCCAMCGVWGNSENTQIEV
CCCCCCCCCCCCCCEEEECCCCCEEEECHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEE
DHKDGRKNDPRVSDLSMQTLDDFQALCKACNDKKRQICKECKETGYRFDATKILGNHYPF
ECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCHHHHHCCCCCC
YEGEAEYDGCVGCYQYDPIQYKKTCNDRIYNEGYQKGYDEGYQIGYHQKTTL
CCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCC
>Mature Secondary Structure
MEFDKEQTPRNSIDRIRLNGYNTKCVFNQSIRQDIKNHYSQQCCAMCGVWGNSENTQIEV
CCCCCCCCCCCCCCEEEECCCCCEEEECHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEE
DHKDGRKNDPRVSDLSMQTLDDFQALCKACNDKKRQICKECKETGYRFDATKILGNHYPF
ECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCHHHHHCCCCCC
YEGEAEYDGCVGCYQYDPIQYKKTCNDRIYNEGYQKGYDEGYQIGYHQKTTL
CCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8996109 [H]