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The map label for this gene is folC [H]

Identifier: 109946909

GI number: 109946909

Start: 248912

End: 250072

Strand: Direct

Name: folC [H]

Synonym: Hac_0295

Alternate gene names: 109946909

Gene position: 248912-250072 (Clockwise)

Preceding gene: 109946908

Following gene: 109946910

Centisome position: 16.02

GC content: 35.92

Gene sequence:

>1161_bases
TTGAAAGGATTGAAGGCGTTTTTAGAAACAAAGCCTAAAGAATACCATAAATTTGACCCTAGCCGTTTCATTCAAATTTA
CAAGGATTTTAGAAACGCTTTTTTTGAGATTCAAGCAAAAGCCATTCACATTGTAGGGACGAATGGTAAGGGTAGCACAG
GGCGGTTTTTAACCCTTTTATTAGCCGATCGAGGGTTTAAGGTGTTGCACTTCACTTCACCGCATGTTTTTGAATTTAGA
GAACGCTTTTATTTGAATGGTTCTATCGTTAATGAAAGCGCTTTAGAAAACGCCCACCAGCAATTGCAACTGCACGCTTT
CAGTAACGCTTGTTCGTATTTTGAATACGCTACCTTACTGGCTGTCATGTTGGCTAAAGATTGCGATTATCTAGTTTTAG
AAGCAGGGCTTGGGGGGGAGTTTGACAGCACAAACGCTTTAGAAAAAACGCTGAGTGTTTTCACCCCCATTGACTATGAT
CATAAGGAATTTTTAGGAGATAGTTTAGAAAGCATTGCAACCACCAAATTAAAGGCGATGAGCACTCTTAATATTGTCGC
TTTGCAACAAGAACTAGTTTTAAATATAGCTCAAAAAATAGCCAAAGACAAACAAGCCCAATTAATTGTAGTTCAAAATG
AGATCTCACAAGAAGTGAAAGACTATATTGAACGCTACCATTTAGCCCATTTTTTAGCGATGAATTTAGAAGTGGCTTTG
AAGGCGTTTGAAACGCTTATGCCATGCAATAAACAAGAAGTTTTACAACATTTAAAGCCTTTGGATTTAATCGGTCGTTG
CGAGCTTTTAAGCCCTAATATTTTAATAGATGTGGGGCATAATCCCCATAGTGCGAAAGCCTTAAAAGAAGAAATCAAGC
GTGTTTTTAACACTAAAATCATTTTAATTTATAATTGCTATCAAGATAAAGACGCTTTTGGGGTGCTAGAAATTTTAAAA
CCGGTAATTAAAAAGGTTTTGATTTTAGAATTAAACAAAGAAAGGGTGATTCAATTAGAAACGCTTCAAGGGATTTTAGA
AAATTTAGGGTTAGAATACGCTTTGTTTGAAGATGTGAAAGAAAACGAAAATTATTTGGTGTATGGCTCATTTTTAGTGG
TCAATGCTTTTTATGAACGCTATCAAAAGAAGAGGGATTGA

Upstream 100 bases:

>100_bases
AACCGCTATTATGCGATCAATCAGGCTGTGGAGCAAACTTTGACTAAATTTGTAGCGCAAATCGCTTATGAAGGGAAATT
CAATAATGAAAAATAGCCCT

Downstream 100 bases:

>100_bases
TGCCGCAAAACCAACTCGTGATCACCATCATTGATGAATCAGGCTCTAAACAACTCAAATTCTCTAAAAATTTAAAACGC
AACCTCATCATTGCTATTGC

Product: folylpolyglutamate synthase / dihydrofolate synthase

Products: NA

Alternate protein names: Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolylpolyglutamate synthase; Tetrahydrofolate synthase [H]

Number of amino acids: Translated: 386; Mature: 386

Protein sequence:

>386_residues
MKGLKAFLETKPKEYHKFDPSRFIQIYKDFRNAFFEIQAKAIHIVGTNGKGSTGRFLTLLLADRGFKVLHFTSPHVFEFR
ERFYLNGSIVNESALENAHQQLQLHAFSNACSYFEYATLLAVMLAKDCDYLVLEAGLGGEFDSTNALEKTLSVFTPIDYD
HKEFLGDSLESIATTKLKAMSTLNIVALQQELVLNIAQKIAKDKQAQLIVVQNEISQEVKDYIERYHLAHFLAMNLEVAL
KAFETLMPCNKQEVLQHLKPLDLIGRCELLSPNILIDVGHNPHSAKALKEEIKRVFNTKIILIYNCYQDKDAFGVLEILK
PVIKKVLILELNKERVIQLETLQGILENLGLEYALFEDVKENENYLVYGSFLVVNAFYERYQKKRD

Sequences:

>Translated_386_residues
MKGLKAFLETKPKEYHKFDPSRFIQIYKDFRNAFFEIQAKAIHIVGTNGKGSTGRFLTLLLADRGFKVLHFTSPHVFEFR
ERFYLNGSIVNESALENAHQQLQLHAFSNACSYFEYATLLAVMLAKDCDYLVLEAGLGGEFDSTNALEKTLSVFTPIDYD
HKEFLGDSLESIATTKLKAMSTLNIVALQQELVLNIAQKIAKDKQAQLIVVQNEISQEVKDYIERYHLAHFLAMNLEVAL
KAFETLMPCNKQEVLQHLKPLDLIGRCELLSPNILIDVGHNPHSAKALKEEIKRVFNTKIILIYNCYQDKDAFGVLEILK
PVIKKVLILELNKERVIQLETLQGILENLGLEYALFEDVKENENYLVYGSFLVVNAFYERYQKKRD
>Mature_386_residues
MKGLKAFLETKPKEYHKFDPSRFIQIYKDFRNAFFEIQAKAIHIVGTNGKGSTGRFLTLLLADRGFKVLHFTSPHVFEFR
ERFYLNGSIVNESALENAHQQLQLHAFSNACSYFEYATLLAVMLAKDCDYLVLEAGLGGEFDSTNALEKTLSVFTPIDYD
HKEFLGDSLESIATTKLKAMSTLNIVALQQELVLNIAQKIAKDKQAQLIVVQNEISQEVKDYIERYHLAHFLAMNLEVAL
KAFETLMPCNKQEVLQHLKPLDLIGRCELLSPNILIDVGHNPHSAKALKEEIKRVFNTKIILIYNCYQDKDAFGVLEILK
PVIKKVLILELNKERVIQLETLQGILENLGLEYALFEDVKENENYLVYGSFLVVNAFYERYQKKRD

Specific function: Conversion of folates to polyglutamate derivatives [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932984, Length=146, Percent_Identity=34.9315068493151, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI66932990, Length=146, Percent_Identity=34.9315068493151, Blast_Score=84, Evalue=3e-16,
Organism=Escherichia coli, GI1788654, Length=368, Percent_Identity=28.804347826087, Blast_Score=138, Evalue=7e-34,
Organism=Caenorhabditis elegans, GI71984923, Length=319, Percent_Identity=25.705329153605, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17553150, Length=319, Percent_Identity=25.705329153605, Blast_Score=93, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17553148, Length=319, Percent_Identity=25.705329153605, Blast_Score=93, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6323760, Length=330, Percent_Identity=25.1515151515152, Blast_Score=90, Evalue=6e-19,
Organism=Saccharomyces cerevisiae, GI6324815, Length=314, Percent_Identity=28.0254777070064, Blast_Score=83, Evalue=6e-17,
Organism=Drosophila melanogaster, GI24641571, Length=179, Percent_Identity=31.8435754189944, Blast_Score=81, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17 [H]

Molecular weight: Translated: 44250; Mature: 44250

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGLKAFLETKPKEYHKFDPSRFIQIYKDFRNAFFEIQAKAIHIVGTNGKGSTGRFLTLL
CCCHHHHHHCCCHHHHCCCHHHHHHHHHHHHHHHHEEEEEEEEEEECCCCCCCCCEEEEE
LADRGFKVLHFTSPHVFEFRERFYLNGSIVNESALENAHQQLQLHAFSNACSYFEYATLL
ECCCCCEEEEECCCHHHHHHHHHEECCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVMLAKDCDYLVLEAGLGGEFDSTNALEKTLSVFTPIDYDHKEFLGDSLESIATTKLKAM
HHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
STLNIVALQQELVLNIAQKIAKDKQAQLIVVQNEISQEVKDYIERYHLAHFLAMNLEVAL
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KAFETLMPCNKQEVLQHLKPLDLIGRCELLSPNILIDVGHNPHSAKALKEEIKRVFNTKI
HHHHHHCCCCHHHHHHHCCCCCHHCCCEEECCCEEEECCCCCCHHHHHHHHHHHHHCCEE
ILIYNCYQDKDAFGVLEILKPVIKKVLILELNKERVIQLETLQGILENLGLEYALFEDVK
EEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHEEHHHHHHHHHHCCCCHHHHHHHH
ENENYLVYGSFLVVNAFYERYQKKRD
CCCCEEEEEHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKGLKAFLETKPKEYHKFDPSRFIQIYKDFRNAFFEIQAKAIHIVGTNGKGSTGRFLTLL
CCCHHHHHHCCCHHHHCCCHHHHHHHHHHHHHHHHEEEEEEEEEEECCCCCCCCCEEEEE
LADRGFKVLHFTSPHVFEFRERFYLNGSIVNESALENAHQQLQLHAFSNACSYFEYATLL
ECCCCCEEEEECCCHHHHHHHHHEECCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVMLAKDCDYLVLEAGLGGEFDSTNALEKTLSVFTPIDYDHKEFLGDSLESIATTKLKAM
HHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
STLNIVALQQELVLNIAQKIAKDKQAQLIVVQNEISQEVKDYIERYHLAHFLAMNLEVAL
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KAFETLMPCNKQEVLQHLKPLDLIGRCELLSPNILIDVGHNPHSAKALKEEIKRVFNTKI
HHHHHHCCCCHHHHHHHCCCCCHHCCCEEECCCEEEECCCCCCHHHHHHHHHHHHHCCEE
ILIYNCYQDKDAFGVLEILKPVIKKVLILELNKERVIQLETLQGILENLGLEYALFEDVK
EEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHEEHHHHHHHHHHCCCCHHHHHHHH
ENENYLVYGSFLVVNAFYERYQKKRD
CCCCEEEEEHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]