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The map label for this gene is cynT [H]

Identifier: 109946874

GI number: 109946874

Start: 212029

End: 212694

Strand: Reverse

Name: cynT [H]

Synonym: Hac_0257

Alternate gene names: 109946874

Gene position: 212694-212029 (Counterclockwise)

Preceding gene: 109946877

Following gene: 109946873

Centisome position: 13.69

GC content: 35.59

Gene sequence:

>666_bases
GTGAAAGCATTTTTAGGGGTGCTAGAGTTTCAAGAGAATGAGTATGAAGAGCTTAAAGAGCTTTGTGAAAGCTTAAAGAC
TAAGCAAAAGCCTCATACTTTGTTTATTTCTTGCGTGAATTCACGAGTCGTGCCCAATCTAATCACAGGCACAAAACCTG
GTGAATTGTATGTGATCCGTAACATGGGCAATGTGATCCCCTCTCAAACAAGCTATAAAGAATCCCTTTCTACCATTGCG
AGCATTGAATACGCTATCATGCATGCGGGCATTCAAAATGTTATTATTTGTGGGCATAGTAATTGTGGGGCTTGCGAGAG
CATTCATTTAATTGATAATAAAACCACAAAAGTTAAAACCCCTTATACCACAAACTGGATACAATTTTTAGAGCCTATTA
AAAAAGAATTAAAAGATCACCCACAATTTAGCAGCCATTCTGCAAGGCGATCATGGTTTACGGAGCGTTTGAATGTGCAC
TTACAACTCAACAACCTCTTAAGCTATGATTTTATCCAAGAAAAAGTGATCAAGAATGAATTGAAAATTTTTGGTTGGCA
TTATATAATAGAGACAGGCAAGATTTATAATTATAATTTTGAAAGCCATTTTTTTGAACCCATTGAAGAAACCATTAAAC
AAAGGATAAACCATGAAAATCTCTAA

Upstream 100 bases:

>100_bases
AATAACGCCATTATAACAAAAAGAAATGAAAGAATTTTAGCTATGATAAGCGTTTGAAAATAAAACAAAGTTTCATGAAA
TTAGATTTAAGGGTTAAAGA

Downstream 100 bases:

>100_bases
AACAAAAACCCCTAAACTAGTTTTAATCGCTGGACCATGCGTCATTGAGAGTTTAGACAATCTAAGAGGTATTGCTATTA
AGTTACAACCCCTAGCCAAC

Product: hypothetical protein

Products: NA

Alternate protein names: Carbonate dehydratase [H]

Number of amino acids: Translated: 221; Mature: 221

Protein sequence:

>221_residues
MKAFLGVLEFQENEYEELKELCESLKTKQKPHTLFISCVNSRVVPNLITGTKPGELYVIRNMGNVIPSQTSYKESLSTIA
SIEYAIMHAGIQNVIICGHSNCGACESIHLIDNKTTKVKTPYTTNWIQFLEPIKKELKDHPQFSSHSARRSWFTERLNVH
LQLNNLLSYDFIQEKVIKNELKIFGWHYIIETGKIYNYNFESHFFEPIEETIKQRINHENL

Sequences:

>Translated_221_residues
MKAFLGVLEFQENEYEELKELCESLKTKQKPHTLFISCVNSRVVPNLITGTKPGELYVIRNMGNVIPSQTSYKESLSTIA
SIEYAIMHAGIQNVIICGHSNCGACESIHLIDNKTTKVKTPYTTNWIQFLEPIKKELKDHPQFSSHSARRSWFTERLNVH
LQLNNLLSYDFIQEKVIKNELKIFGWHYIIETGKIYNYNFESHFFEPIEETIKQRINHENL
>Mature_221_residues
MKAFLGVLEFQENEYEELKELCESLKTKQKPHTLFISCVNSRVVPNLITGTKPGELYVIRNMGNVIPSQTSYKESLSTIA
SIEYAIMHAGIQNVIICGHSNCGACESIHLIDNKTTKVKTPYTTNWIQFLEPIKKELKDHPQFSSHSARRSWFTERLNVH
LQLNNLLSYDFIQEKVIKNELKIFGWHYIIETGKIYNYNFESHFFEPIEETIKQRINHENL

Specific function: Reversible Hydration Of Carbon Dioxide. Carbon Dioxide Formed In The Bicarbonate-Dependent Decomposition Of Cyanate By Cyanase (Cyns) Diffuses Out Of The Cell Faster Than It Would Be Hydrated To Bicarbonate, So The Apparent Function Of This Enzyme Is To C

COG id: COG0288

COG function: function code P; Carbonic anhydrase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the beta-class carbonic anhydrase family [H]

Homologues:

Organism=Escherichia coli, GI1786534, Length=208, Percent_Identity=34.1346153846154, Blast_Score=133, Evalue=9e-33,
Organism=Escherichia coli, GI1786318, Length=211, Percent_Identity=28.9099526066351, Blast_Score=76, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI25147564, Length=238, Percent_Identity=26.0504201680672, Blast_Score=88, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI115532990, Length=237, Percent_Identity=25.3164556962025, Blast_Score=84, Evalue=6e-17,
Organism=Saccharomyces cerevisiae, GI6324292, Length=168, Percent_Identity=29.7619047619048, Blast_Score=80, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24645213, Length=244, Percent_Identity=23.3606557377049, Blast_Score=94, Evalue=5e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001765
- InterPro:   IPR015892 [H]

Pfam domain/function: PF00484 Pro_CA [H]

EC number: =4.2.1.1 [H]

Molecular weight: Translated: 25738; Mature: 25738

Theoretical pI: Translated: 7.31; Mature: 7.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAFLGVLEFQENEYEELKELCESLKTKQKPHTLFISCVNSRVVPNLITGTKPGELYVIR
CCHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHCCCCCCCEEEEC
NMGNVIPSQTSYKESLSTIASIEYAIMHAGIQNVIICGHSNCGACESIHLIDNKTTKVKT
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCEEEEECCCCCEEEC
PYTTNWIQFLEPIKKELKDHPQFSSHSARRSWFTERLNVHLQLNNLLSYDFIQEKVIKNE
CCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHEEEEEECCHHHHHHHHHHHHHHH
LKIFGWHYIIETGKIYNYNFESHFFEPIEETIKQRINHENL
HHHEEEEEEEECCEEEEECCHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MKAFLGVLEFQENEYEELKELCESLKTKQKPHTLFISCVNSRVVPNLITGTKPGELYVIR
CCHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHCCCCCCCEEEEC
NMGNVIPSQTSYKESLSTIASIEYAIMHAGIQNVIICGHSNCGACESIHLIDNKTTKVKT
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCEEEEECCCCCEEEC
PYTTNWIQFLEPIKKELKDHPQFSSHSARRSWFTERLNVHLQLNNLLSYDFIQEKVIKNE
CCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHEEEEEECCHHHHHHHHHHHHHHH
LKIFGWHYIIETGKIYNYNFESHFFEPIEETIKQRINHENL
HHHEEEEEEEECCEEEEECCHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]