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The map label for this gene is kdsA

Identifier: 109946873

GI number: 109946873

Start: 211212

End: 212042

Strand: Reverse

Name: kdsA

Synonym: Hac_0256

Alternate gene names: 109946873

Gene position: 212042-211212 (Counterclockwise)

Preceding gene: 109946874

Following gene: 109946872

Centisome position: 13.65

GC content: 40.07

Gene sequence:

>831_bases
ATGAAAATCTCTAAAACAAAAACCCCTAAACTAGTTTTAATCGCTGGACCATGCGTCATTGAGAGTTTAGACAATCTAAG
AGGTATTGCTATTAAGTTACAACCCCTAGCCAACAATGAGCGATTGGATTTTTATTTTAAAGCGAGTTTTGATAAGGCTA
ACCGCACAAGTTTAGAAAGCTATAGAGGGCCCGGACTAGAAAAAGGCTTAGAAATGTTACAAGCTATCAAAGATGAATTT
GGCTATAAGATTTTAACCGATGTGCATGAGAGCTATCAAGTAAGCATAGCGGTCAAAGTGGCTGATATTTTACAAATACC
GGCGTTTTTATGCCGACAAACGGATTTGATTGTAGAAGTGAGTCAAACTAACGCCATTATCAATATTAAAAAAGGGCAAT
TCATGAGCCCAAAAGACATGCAATATTCTGTCTTAAAAGCCCTTAAAACAAGAGATAAAAATATTCAAAACCCCACTTAT
GAAAACGCTTTAGCTAATGGCGTGTGGCTGTGTGAAAGGGGGAGCAGCTTTGGGTATGGGAATTTAGTGGTGGATATGCG
TTCTTTAAAAATCATGCGAGAATTTGCCCCGGTGATTTTTGACGCCACTCATAGCGTGCAAATGCCAGGGGGTGCGAATG
GGAAAAGTTCAGGAGAAAGCTCTTTTGCCCCTATTTTAGCCAGAGCGGCGGCTGCGGTGGGTGTTGATGGGCTATTTGCA
GAAACGCATATTGATCCTAAAAACGCCCTAAGCGATGGGGCTAACATGCTCAAACCTAATGAGCTTGAAAGCTTGGTAAC
CGATATGTTAAAAATCCAAAATTTATTTTAA

Upstream 100 bases:

>100_bases
GGTTGGCATTATATAATAGAGACAGGCAAGATTTATAATTATAATTTTGAAAGCCATTTTTTTGAACCCATTGAAGAAAC
CATTAAACAAAGGATAAACC

Downstream 100 bases:

>100_bases
AGGAGTTTTCATGCAACTTATAGAAGGGAAATTACAACTACAAGGGAATGAAAGAATCGCTATTGTAATCTCGCGCTTCA
ATCACATTATCACAGACAGA

Product: 2-dehydro-3-deoxyphosphooctonate aldolase

Products: NA

Alternate protein names: 3-deoxy-D-manno-octulosonic acid 8-phosphate synthase; KDO-8-phosphate synthase; KDO 8-P synthase; KDOPS; Phospho-2-dehydro-3-deoxyoctonate aldolase

Number of amino acids: Translated: 276; Mature: 276

Protein sequence:

>276_residues
MKISKTKTPKLVLIAGPCVIESLDNLRGIAIKLQPLANNERLDFYFKASFDKANRTSLESYRGPGLEKGLEMLQAIKDEF
GYKILTDVHESYQVSIAVKVADILQIPAFLCRQTDLIVEVSQTNAIINIKKGQFMSPKDMQYSVLKALKTRDKNIQNPTY
ENALANGVWLCERGSSFGYGNLVVDMRSLKIMREFAPVIFDATHSVQMPGGANGKSSGESSFAPILARAAAAVGVDGLFA
ETHIDPKNALSDGANMLKPNELESLVTDMLKIQNLF

Sequences:

>Translated_276_residues
MKISKTKTPKLVLIAGPCVIESLDNLRGIAIKLQPLANNERLDFYFKASFDKANRTSLESYRGPGLEKGLEMLQAIKDEF
GYKILTDVHESYQVSIAVKVADILQIPAFLCRQTDLIVEVSQTNAIINIKKGQFMSPKDMQYSVLKALKTRDKNIQNPTY
ENALANGVWLCERGSSFGYGNLVVDMRSLKIMREFAPVIFDATHSVQMPGGANGKSSGESSFAPILARAAAAVGVDGLFA
ETHIDPKNALSDGANMLKPNELESLVTDMLKIQNLF
>Mature_276_residues
MKISKTKTPKLVLIAGPCVIESLDNLRGIAIKLQPLANNERLDFYFKASFDKANRTSLESYRGPGLEKGLEMLQAIKDEF
GYKILTDVHESYQVSIAVKVADILQIPAFLCRQTDLIVEVSQTNAIINIKKGQFMSPKDMQYSVLKALKTRDKNIQNPTY
ENALANGVWLCERGSSFGYGNLVVDMRSLKIMREFAPVIFDATHSVQMPGGANGKSSGESSFAPILARAAAAVGVDGLFA
ETHIDPKNALSDGANMLKPNELESLVTDMLKIQNLF

Specific function: Synthesis Of Kdo 8-P Which Is Required For Lipid A Maturation And Cellular Growth. [C]

COG id: COG2877

COG function: function code M; 3-deoxy-D-manno-octulosonic acid (KDO) 8-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the kdsA family

Homologues:

Organism=Escherichia coli, GI1787466, Length=268, Percent_Identity=42.5373134328358, Blast_Score=203, Evalue=1e-53,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): KDSA_HELAH (Q17Z24)

Other databases:

- EMBL:   AM260522
- RefSeq:   YP_664101.1
- ProteinModelPortal:   Q17Z24
- SMR:   Q17Z24
- STRING:   Q17Z24
- GeneID:   4176132
- GenomeReviews:   AM260522_GR
- KEGG:   hac:Hac_0256
- NMPDR:   fig|382638.8.peg.247
- eggNOG:   COG2877
- HOGENOM:   HBG575990
- OMA:   ESEENIF
- PhylomeDB:   Q17Z24
- ProtClustDB:   PRK05198
- BioCyc:   HACI382638:HAC_0256-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00056
- InterPro:   IPR013785
- InterPro:   IPR006218
- InterPro:   IPR006269
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR21057:SF2
- TIGRFAMs:   TIGR01362

Pfam domain/function: PF00793 DAHP_synth_1

EC number: =2.5.1.55

Molecular weight: Translated: 30347; Mature: 30347

Theoretical pI: Translated: 8.43; Mature: 8.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKISKTKTPKLVLIAGPCVIESLDNLRGIAIKLQPLANNERLDFYFKASFDKANRTSLES
CCCCCCCCCEEEEEECCHHHHHHHCCCEEEEEEEECCCCCEEEEEEEECCCCCCHHHHHH
YRGPGLEKGLEMLQAIKDEFGYKILTDVHESYQVSIAVKVADILQIPAFLCRQTDLIVEV
HCCCCHHHHHHHHHHHHHHHCCCHHHHHCCCEEEEEEEEHHHHHHHHHHHHCCCCEEEEE
SQTNAIINIKKGQFMSPKDMQYSVLKALKTRDKNIQNPTYENALANGVWLCERGSSFGYG
ECCCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHCCEEEEECCCCCCCC
NLVVDMRSLKIMREFAPVIFDATHSVQMPGGANGKSSGESSFAPILARAAAAVGVDGLFA
CEEEEHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEH
ETHIDPKNALSDGANMLKPNELESLVTDMLKIQNLF
CCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKISKTKTPKLVLIAGPCVIESLDNLRGIAIKLQPLANNERLDFYFKASFDKANRTSLES
CCCCCCCCCEEEEEECCHHHHHHHCCCEEEEEEEECCCCCEEEEEEEECCCCCCHHHHHH
YRGPGLEKGLEMLQAIKDEFGYKILTDVHESYQVSIAVKVADILQIPAFLCRQTDLIVEV
HCCCCHHHHHHHHHHHHHHHCCCHHHHHCCCEEEEEEEEHHHHHHHHHHHHCCCCEEEEE
SQTNAIINIKKGQFMSPKDMQYSVLKALKTRDKNIQNPTYENALANGVWLCERGSSFGYG
ECCCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHCCEEEEECCCCCCCC
NLVVDMRSLKIMREFAPVIFDATHSVQMPGGANGKSSGESSFAPILARAAAAVGVDGLFA
CEEEEHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEH
ETHIDPKNALSDGANMLKPNELESLVTDMLKIQNLF
CCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA