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The map label for this gene is smpB

Identifier: 109946797

GI number: 109946797

Start: 148433

End: 148891

Strand: Reverse

Name: smpB

Synonym: Hac_0174

Alternate gene names: 109946797

Gene position: 148891-148433 (Counterclockwise)

Preceding gene: 109946798

Following gene: 109946796

Centisome position: 9.58

GC content: 31.15

Gene sequence:

>459_bases
ATGAAACTTATTGCCAGCAATAAAAAAGCCTATTTTGATTATGAAATTTTGGAGACTTTAGAAGCAGGATTGGTGCTTTT
AGGCTCTGAAGTCAAGGCTTTGAGACAAACTAGGGTGAATTTAAAGGATAATTTTGTTAAAATAATCAAAGGAGAAGCTT
TTTTATTTGGTGTTCATATATCCTATTTAGATACAATCCATGCGTATTATAAGCCCAATGAAAGGCGAGAGCGTAAGTTG
CTTTTACATAAAAAGCAATTATTGAAATGGCAAATTGAAGCGTCTAAAGAACGCTTGAGTATTGTAGGATTAAAATTGTA
TTTTAACCAAAGAAATAGAGCTAAAATCCAAATTGCTTTAGTGAAAGGAAAAAAATTGCACGATAAAAGACAAAACCTCA
AAGAAAAAGCACTCAATAAAGAAATTCTTGCCGATTTAAAACACCACTTTAAAGGATAA

Upstream 100 bases:

>100_bases
AAGCTTTAAAGGATATAGAGAGCCAATTGGGTAAGGAGTGGTTTTTTAGCGGGAGTGGGAGCGCGTTTTTTAGGCTAAAG
CCCACTCAAAAAGGGAGCGA

Downstream 100 bases:

>100_bases
TAATGAGAGAAATGGTAGATTATGGGATCATAGGTTTTTTGATATTCTTATCGGTCATCGTTATAGCGATCGCAATAGAA
AGGATGTGGTTTTTTGCCAC

Product: SsrA-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 152; Mature: 152

Protein sequence:

>152_residues
MKLIASNKKAYFDYEILETLEAGLVLLGSEVKALRQTRVNLKDNFVKIIKGEAFLFGVHISYLDTIHAYYKPNERRERKL
LLHKKQLLKWQIEASKERLSIVGLKLYFNQRNRAKIQIALVKGKKLHDKRQNLKEKALNKEILADLKHHFKG

Sequences:

>Translated_152_residues
MKLIASNKKAYFDYEILETLEAGLVLLGSEVKALRQTRVNLKDNFVKIIKGEAFLFGVHISYLDTIHAYYKPNERRERKL
LLHKKQLLKWQIEASKERLSIVGLKLYFNQRNRAKIQIALVKGKKLHDKRQNLKEKALNKEILADLKHHFKG
>Mature_152_residues
MKLIASNKKAYFDYEILETLEAGLVLLGSEVKALRQTRVNLKDNFVKIIKGEAFLFGVHISYLDTIHAYYKPNERRERKL
LLHKKQLLKWQIEASKERLSIVGLKLYFNQRNRAKIQIALVKGKKLHDKRQNLKEKALNKEILADLKHHFKG

Specific function: Binds specifically to the ssrA RNA (tmRNA) and is required for stable association of ssrA with ribosomes

COG id: COG0691

COG function: function code O; tmRNA-binding protein

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the smpB family

Homologues:

Organism=Escherichia coli, GI1788973, Length=133, Percent_Identity=40.6015037593985, Blast_Score=97, Evalue=3e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SSRP_HELAH (Q17ZA0)

Other databases:

- EMBL:   AM260522
- RefSeq:   YP_664025.1
- ProteinModelPortal:   Q17ZA0
- SMR:   Q17ZA0
- STRING:   Q17ZA0
- GeneID:   4177458
- GenomeReviews:   AM260522_GR
- KEGG:   hac:Hac_0174
- NMPDR:   fig|382638.8.peg.171
- eggNOG:   COG0691
- HOGENOM:   HBG293422
- OMA:   EKYEAGI
- ProtClustDB:   PRK05422
- BioCyc:   HACI382638:HAC_0174-MONOMER
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_00023
- InterPro:   IPR000037
- InterPro:   IPR020081
- Gene3D:   G3DSA:2.40.280.10
- ProDom:   PD004488
- TIGRFAMs:   TIGR00086

Pfam domain/function: PF01668 SmpB; SSF74982 SmpB

EC number: NA

Molecular weight: Translated: 17851; Mature: 17851

Theoretical pI: Translated: 10.73; Mature: 10.73

Prosite motif: PS01317 SSRP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
0.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
0.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLIASNKKAYFDYEILETLEAGLVLLGSEVKALRQTRVNLKDNFVKIIKGEAFLFGVHI
CCCCCCCCCCEEHHHHHHHHHHCCEEECHHHHHHHHHHCCCCHHHHEEECCCEEEEEEHH
SYLDTIHAYYKPNERRERKLLLHKKQLLKWQIEASKERLSIVGLKLYFNQRNRAKIQIAL
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCCEEEEEEE
VKGKKLHDKRQNLKEKALNKEILADLKHHFKG
ECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKLIASNKKAYFDYEILETLEAGLVLLGSEVKALRQTRVNLKDNFVKIIKGEAFLFGVHI
CCCCCCCCCCEEHHHHHHHHHHCCEEECHHHHHHHHHHCCCCHHHHEEECCCEEEEEEHH
SYLDTIHAYYKPNERRERKLLLHKKQLLKWQIEASKERLSIVGLKLYFNQRNRAKIQIAL
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCCEEEEEEE
VKGKKLHDKRQNLKEKALNKEILADLKHHFKG
ECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA