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The map label for this gene is rpoA

Identifier: 109946783

GI number: 109946783

Start: 137014

End: 138048

Strand: Direct

Name: rpoA

Synonym: Hac_0160

Alternate gene names: 109946783

Gene position: 137014-138048 (Clockwise)

Preceding gene: 109946782

Following gene: 109946784

Centisome position: 8.82

GC content: 38.65

Gene sequence:

>1035_bases
ATGAAAGTTATCAAAACAGCACCTTTGATCCCATCAGAAATTAAGGTGCTAGAGAAAGAGGGCAATCGGATTAAGATTTC
TCTAGCTCCATTTGAGTTTGGTTACGCTGTTACGCTCGCTCATCCTATTAGAAGGCTCTTGCTTTTAAGTTCCGTGGGGT
ATGCTCCTGTGGGTTTAAAGATTGAAGGCGTCCATCATGAGTTTGATTCATTAAGAGGCGTTACTGAAGATGTGTCGCTT
TTTATCATGAATTTAAAGAATATCCGTTTTATAGCCAAGGCGTTAGTGGGGCAGGATAGCCCTTTGGAAAACCAATCGGT
TGTGGTGGATTATTCTTTTAAAGGGCCTATGGAGCTTAGGGCTAGGGATTTAAATTCTGAGCATATAGAAATCATCAATC
CTGAAATGCTCCTAGCCACGATCAACGAAGACGCTCAATTGAATTTTTCGCTCATCATTTATAAAGGAATGGGGTATGTC
CCAAGCGAAATTACAAGAGAATTGATGCCTGAAGGTTACATGCCTTTAGATGGATCTTTTACGCCGATTAAAAATGTGGT
TTATGAGATTGAAAATGTTTTGGTTGAGGGCGATCCTAACTATGAAAAAATCATTTTTGATATTGAAACAGATGGGCAAA
TTGATCCTTACAAAGCGTTTTTATCAGCGGTGAAAGTGATGAGCAAGCAACTAGGCGTTTTTGGTGAAAAACCCATTGCT
AACACAGAGTATTCAGGCGATTACGCCCAAAGAGATGATGCTAAAGATTTGAGCGCTAAGATTGAAAGTATGAATTTGAG
CGCTAGGTGTTTTAATTGCTTGGATAAAATCGGTATCAAGTATGTGGGCGAACTGGTTTTAATGAGCGAAGAAGAGCTTA
AAGGCGTGAAAAACATGGGCAAAAAATCCTATGATGAAATCGCTGAAAAATTGAATGATTTAGGCTATCCTGTAGGCACA
GAATTAAGCCTTGAACAAAGAGAGAGTTTGAAAAAAAGATTAGAAAAATTAGAAGATAAAGGAGGTAATGACTGA

Upstream 100 bases:

>100_bases
AATATGGCATCTTCACCCGCTACCCTGAAAGAGAAGAAGTGGTTGTTCCTATTGAAGAAAGACTCATTGTAGAATTGTAT
TCTAAGTAAGGGGTTAGAGC

Downstream 100 bases:

>100_bases
TGAGACACAAACACGGATACCGCAAGCTTGGGAGAACTAGCTCGCACAGGAAGGCATTATTAAAGAATTTAGCTATCGCT
TTGATTGAGCATAATAAAAT

Product: DNA-directed RNA polymerase subunit alpha

Products: NA

Alternate protein names: RNAP subunit alpha; RNA polymerase subunit alpha; Transcriptase subunit alpha

Number of amino acids: Translated: 344; Mature: 344

Protein sequence:

>344_residues
MKVIKTAPLIPSEIKVLEKEGNRIKISLAPFEFGYAVTLAHPIRRLLLLSSVGYAPVGLKIEGVHHEFDSLRGVTEDVSL
FIMNLKNIRFIAKALVGQDSPLENQSVVVDYSFKGPMELRARDLNSEHIEIINPEMLLATINEDAQLNFSLIIYKGMGYV
PSEITRELMPEGYMPLDGSFTPIKNVVYEIENVLVEGDPNYEKIIFDIETDGQIDPYKAFLSAVKVMSKQLGVFGEKPIA
NTEYSGDYAQRDDAKDLSAKIESMNLSARCFNCLDKIGIKYVGELVLMSEEELKGVKNMGKKSYDEIAEKLNDLGYPVGT
ELSLEQRESLKKRLEKLEDKGGND

Sequences:

>Translated_344_residues
MKVIKTAPLIPSEIKVLEKEGNRIKISLAPFEFGYAVTLAHPIRRLLLLSSVGYAPVGLKIEGVHHEFDSLRGVTEDVSL
FIMNLKNIRFIAKALVGQDSPLENQSVVVDYSFKGPMELRARDLNSEHIEIINPEMLLATINEDAQLNFSLIIYKGMGYV
PSEITRELMPEGYMPLDGSFTPIKNVVYEIENVLVEGDPNYEKIIFDIETDGQIDPYKAFLSAVKVMSKQLGVFGEKPIA
NTEYSGDYAQRDDAKDLSAKIESMNLSARCFNCLDKIGIKYVGELVLMSEEELKGVKNMGKKSYDEIAEKLNDLGYPVGT
ELSLEQRESLKKRLEKLEDKGGND
>Mature_344_residues
MKVIKTAPLIPSEIKVLEKEGNRIKISLAPFEFGYAVTLAHPIRRLLLLSSVGYAPVGLKIEGVHHEFDSLRGVTEDVSL
FIMNLKNIRFIAKALVGQDSPLENQSVVVDYSFKGPMELRARDLNSEHIEIINPEMLLATINEDAQLNFSLIIYKGMGYV
PSEITRELMPEGYMPLDGSFTPIKNVVYEIENVLVEGDPNYEKIIFDIETDGQIDPYKAFLSAVKVMSKQLGVFGEKPIA
NTEYSGDYAQRDDAKDLSAKIESMNLSARCFNCLDKIGIKYVGELVLMSEEELKGVKNMGKKSYDEIAEKLNDLGYPVGT
ELSLEQRESLKKRLEKLEDKGGND

Specific function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates

COG id: COG0202

COG function: function code K; DNA-directed RNA polymerase, alpha subunit/40 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA polymerase alpha chain family

Homologues:

Organism=Escherichia coli, GI1789690, Length=322, Percent_Identity=29.5031055900621, Blast_Score=142, Evalue=4e-35,

Paralogues:

None

Copy number: 850 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 560 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 6847 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 7,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): RPOA_HELAH (Q17ZB4)

Other databases:

- EMBL:   AM260522
- RefSeq:   YP_664011.1
- ProteinModelPortal:   Q17ZB4
- SMR:   Q17ZB4
- STRING:   Q17ZB4
- GeneID:   4176862
- GenomeReviews:   AM260522_GR
- KEGG:   hac:Hac_0160
- NMPDR:   fig|382638.8.peg.157
- eggNOG:   COG0202
- HOGENOM:   HBG430844
- OMA:   FGTTLGN
- ProtClustDB:   PRK05182
- BioCyc:   HACI382638:HAC_0160-MONOMER
- HAMAP:   MF_00059
- InterPro:   IPR011261
- InterPro:   IPR011262
- InterPro:   IPR009025
- InterPro:   IPR011263
- InterPro:   IPR011260
- InterPro:   IPR011773
- Gene3D:   G3DSA:2.170.120.12
- ProDom:   PD001179
- SMART:   SM00662
- TIGRFAMs:   TIGR02027

Pfam domain/function: PF01000 RNA_pol_A_bac; PF03118 RNA_pol_A_CTD; PF01193 RNA_pol_L; SSF47789 RNAP_alpha_C; SSF56553 RNAP_insert; SSF55257 RNAP_RBP11-like

EC number: =2.7.7.6

Molecular weight: Translated: 38536; Mature: 38536

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVIKTAPLIPSEIKVLEKEGNRIKISLAPFEFGYAVTLAHPIRRLLLLSSVGYAPVGLK
CCCCCCCCCCHHHHHHHHCCCCEEEEEECCCCCCCEEHHHHHHHHHHHHHHCCCCCCCEE
IEGVHHEFDSLRGVTEDVSLFIMNLKNIRFIAKALVGQDSPLENQSVVVDYSFKGPMELR
EECCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCHHHH
ARDLNSEHIEIINPEMLLATINEDAQLNFSLIIYKGMGYVPSEITRELMPEGYMPLDGSF
HHCCCCCCEEEECCEEEEEEECCCCEEEEEEEEEECCCCCHHHHHHHHCCCCCCCCCCCC
TPIKNVVYEIENVLVEGDPNYEKIIFDIETDGQIDPYKAFLSAVKVMSKQLGVFGEKPIA
CHHHHHHHHHHHHEECCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCC
NTEYSGDYAQRDDAKDLSAKIESMNLSARCFNCLDKIGIKYVGELVLMSEEELKGVKNMG
CCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHEECHHHHHHHHHCC
KKSYDEIAEKLNDLGYPVGTELSLEQRESLKKRLEKLEDKGGND
CCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKVIKTAPLIPSEIKVLEKEGNRIKISLAPFEFGYAVTLAHPIRRLLLLSSVGYAPVGLK
CCCCCCCCCCHHHHHHHHCCCCEEEEEECCCCCCCEEHHHHHHHHHHHHHHCCCCCCCEE
IEGVHHEFDSLRGVTEDVSLFIMNLKNIRFIAKALVGQDSPLENQSVVVDYSFKGPMELR
EECCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCHHHH
ARDLNSEHIEIINPEMLLATINEDAQLNFSLIIYKGMGYVPSEITRELMPEGYMPLDGSF
HHCCCCCCEEEECCEEEEEEECCCCEEEEEEEEEECCCCCHHHHHHHHCCCCCCCCCCCC
TPIKNVVYEIENVLVEGDPNYEKIIFDIETDGQIDPYKAFLSAVKVMSKQLGVFGEKPIA
CHHHHHHHHHHHHEECCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCC
NTEYSGDYAQRDDAKDLSAKIESMNLSARCFNCLDKIGIKYVGELVLMSEEELKGVKNMG
CCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHEECHHHHHHHHHCC
KKSYDEIAEKLNDLGYPVGTELSLEQRESLKKRLEKLEDKGGND
CCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA