Click here to switch to the map view.

The map label for this gene is yciL [C]

Identifier: 109946748

GI number: 109946748

Start: 117178

End: 117942

Strand: Direct

Name: yciL [C]

Synonym: Hac_0125

Alternate gene names: 109946748

Gene position: 117178-117942 (Clockwise)

Preceding gene: 109946747

Following gene: 109946749

Centisome position: 7.54

GC content: 39.87

Gene sequence:

>765_bases
TTGAGGATCAACCAATTTTTAGCCCATTATACCAAGCATTCGCGCAGAGAGGCTGAAAAATTGGTTTTAGAGGGGCGGGT
GAAAATCAACCATGAGCATGCCAAACTCGCTAGCGTGGTTAAAGAAAACGACAAGGTTTTTTTGGATAAGCGTCTCATCA
AGCCCTTAAAAAACAAAAAATTCAGCGTGCTAGTTTATCACAAGCCAAAGGGCGAGTTAGTGAGCAAAAACGATCCCTTA
AAACGGCGCATGATTTATGAAAGCTTGGAAAGAAAATACGCGCATTTTGTGCCTGTGGGCCGTTTGGATTTTGCTAGTGA
GGGGGTGCTATTATTAAGCGATAGTAAGGCGGTGGTGAGTGCTTTAATGCATGCAAATTTGGAAAAAGAGTATCTTATTA
AAATTCAAGGTTTTGTTACAAAAGAGATGGAAAACGCTATGCAAGAGGGCTTGAAATTAGAAAACGCCACTAAAGGAGCA
CACCAAAAAACCCCAATTAAAAGCATGGAATTCGCCCCCTTTATTGGTTATGAAATCATCAAAAACCATGCCAAATACTC
TAAAATTAGAGTTGTTATCAATGAGGGGAAAAACAGAGAATTGAGGCGTTTTTTTGCGTTTTTTAACGCTAGAGTGTTGG
ATTTAAGGCGCGTTCGTTATGGTTTTGTGAATTTGAATGCCTTGCCGGTAGGGAAAATGCGTTTTTTAAACCGCCAAGAA
TACAATGAATTGCATGCGTTTATGGCCAATGTTAAAGGGGATTAG

Upstream 100 bases:

>100_bases
TTATCAAAGATAAGGGCAAGCAATTCAAATTCCAAAGCGATTTGTTGGTAGATGAAAAGATTAAGGACGATTTTAAAGAA
TTAGAGTGGAGGGATTTAGC

Downstream 100 bases:

>100_bases
CTTTTGGGTGTAGGGTTTATGCTATACTTTTAAAGTTGGGGGAAATAAAAATTAAGGATTATCATGTTAGAAGTGATTAA
CGGAAAAAATTACGCAGAGA

Product: pseudouridylate synthase

Products: NA

Alternate protein names: RNA pseudouridylate synthase; RNA-uridine isomerase [H]

Number of amino acids: Translated: 254; Mature: 254

Protein sequence:

>254_residues
MRINQFLAHYTKHSRREAEKLVLEGRVKINHEHAKLASVVKENDKVFLDKRLIKPLKNKKFSVLVYHKPKGELVSKNDPL
KRRMIYESLERKYAHFVPVGRLDFASEGVLLLSDSKAVVSALMHANLEKEYLIKIQGFVTKEMENAMQEGLKLENATKGA
HQKTPIKSMEFAPFIGYEIIKNHAKYSKIRVVINEGKNRELRRFFAFFNARVLDLRRVRYGFVNLNALPVGKMRFLNRQE
YNELHAFMANVKGD

Sequences:

>Translated_254_residues
MRINQFLAHYTKHSRREAEKLVLEGRVKINHEHAKLASVVKENDKVFLDKRLIKPLKNKKFSVLVYHKPKGELVSKNDPL
KRRMIYESLERKYAHFVPVGRLDFASEGVLLLSDSKAVVSALMHANLEKEYLIKIQGFVTKEMENAMQEGLKLENATKGA
HQKTPIKSMEFAPFIGYEIIKNHAKYSKIRVVINEGKNRELRRFFAFFNARVLDLRRVRYGFVNLNALPVGKMRFLNRQE
YNELHAFMANVKGD
>Mature_254_residues
MRINQFLAHYTKHSRREAEKLVLEGRVKINHEHAKLASVVKENDKVFLDKRLIKPLKNKKFSVLVYHKPKGELVSKNDPL
KRRMIYESLERKYAHFVPVGRLDFASEGVLLLSDSKAVVSALMHANLEKEYLIKIQGFVTKEMENAMQEGLKLENATKGA
HQKTPIKSMEFAPFIGYEIIKNHAKYSKIRVVINEGKNRELRRFFAFFNARVLDLRRVRYGFVNLNALPVGKMRFLNRQE
YNELHAFMANVKGD

Specific function: Unknown

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787524, Length=255, Percent_Identity=30.1960784313725, Blast_Score=108, Evalue=5e-25,
Organism=Escherichia coli, GI1790453, Length=252, Percent_Identity=28.1746031746032, Blast_Score=96, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 29598; Mature: 29598

Theoretical pI: Translated: 10.66; Mature: 10.66

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRINQFLAHYTKHSRREAEKLVLEGRVKINHEHAKLASVVKENDKVFLDKRLIKPLKNKK
CCHHHHHHHHHHHHHHHHHHHHHHCEEEECHHHHHHHHHHHCCCCEEHHHHHHHHHCCCC
FSVLVYHKPKGELVSKNDPLKRRMIYESLERKYAHFVPVGRLDFASEGVLLLSDSKAVVS
EEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCHHHHH
ALMHANLEKEYLIKIQGFVTKEMENAMQEGLKLENATKGAHQKTPIKSMEFAPFIGYEII
HHHHCCCCHHHEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHH
KNHAKYSKIRVVINEGKNRELRRFFAFFNARVLDLRRVRYGFVNLNALPVGKMRFLNRQE
HCCCHHEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCEEEECCCCCHHHHHHHHHH
YNELHAFMANVKGD
HHHHHHHHHHCCCC
>Mature Secondary Structure
MRINQFLAHYTKHSRREAEKLVLEGRVKINHEHAKLASVVKENDKVFLDKRLIKPLKNKK
CCHHHHHHHHHHHHHHHHHHHHHHCEEEECHHHHHHHHHHHCCCCEEHHHHHHHHHCCCC
FSVLVYHKPKGELVSKNDPLKRRMIYESLERKYAHFVPVGRLDFASEGVLLLSDSKAVVS
EEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCHHHHH
ALMHANLEKEYLIKIQGFVTKEMENAMQEGLKLENATKGAHQKTPIKSMEFAPFIGYEII
HHHHCCCCHHHEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHH
KNHAKYSKIRVVINEGKNRELRRFFAFFNARVLDLRRVRYGFVNLNALPVGKMRFLNRQE
HCCCHHEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCEEEECCCCCHHHHHHHHHH
YNELHAFMANVKGD
HHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9252185 [H]