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The map label for this gene is DDEM fragment 2 [H]

Identifier: 109946722

GI number: 109946722

Start: 87595

End: 87900

Strand: Reverse

Name: DDEM fragment 2 [H]

Synonym: Hac_0093

Alternate gene names: 109946722

Gene position: 87900-87595 (Counterclockwise)

Preceding gene: 109946723

Following gene: 109946721

Centisome position: 5.66

GC content: 34.97

Gene sequence:

>306_bases
GTGAAAGCCATAAAACTAGAAATTTTTATCATTGAAAATGTGAAAAACCTAATCTTATACACTAAAGGCTATTTTTTAGA
AGAGATTAAAGAAAGATTAAACGCTTTAGGGTATCAATTAAGCTATCAAATCCTAAACGCTAAAGATTATGGCGTGCCTC
AAAGCAGGGAGAGAGCTTTTATTGTTGGGGCTACTCATTTTAGTTTTGATTTTAATCTTTTAGAGCCTTCTCAAAGCGTG
AGCGTTCAAGAGGCTATAAGCGATTTAGCCTATTTTCATTCTAATGAGGGGGCGTTTGGCGTTTGA

Upstream 100 bases:

>100_bases
ATTGGTGGGTCTCCCTAATCAAGGCTTTTCTAATTAAAGGGAAAAAATTTAGGGCTAAAAGACCCTAGAAATTTCTTATT
CTTAGGATATTATAGAAATA

Downstream 100 bases:

>100_bases
AAGCGATTATTTAAACCCTATCCAATCCAATTATCAAGCTTTAATGCGAAAAAATAGCCCTAAATTATACAACCATCAAG
CCACCAACCACTCACAAGGC

Product: cytosine specific DNA methyltransferase fragment 2

Products: NA

Alternate protein names: M.BspRI; Cytosine-specific methyltransferase BspRI [H]

Number of amino acids: Translated: 101; Mature: 101

Protein sequence:

>101_residues
MKAIKLEIFIIENVKNLILYTKGYFLEEIKERLNALGYQLSYQILNAKDYGVPQSRERAFIVGATHFSFDFNLLEPSQSV
SVQEAISDLAYFHSNEGAFGV

Sequences:

>Translated_101_residues
MKAIKLEIFIIENVKNLILYTKGYFLEEIKERLNALGYQLSYQILNAKDYGVPQSRERAFIVGATHFSFDFNLLEPSQSV
SVQEAISDLAYFHSNEGAFGV
>Mature_101_residues
MKAIKLEIFIIENVKNLILYTKGYFLEEIKERLNALGYQLSYQILNAKDYGVPQSRERAFIVGATHFSFDFNLLEPSQSV
SVQEAISDLAYFHSNEGAFGV

Specific function: This methylase recognizes the double-stranded sequence GGCC, causes specific methylation on C-3 on both strands, and protects the DNA from cleavage by the BspRI endonuclease [H]

COG id: COG0270

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the C5-methyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018117
- InterPro:   IPR001525 [H]

Pfam domain/function: PF00145 DNA_methylase [H]

EC number: =2.1.1.37 [H]

Molecular weight: Translated: 11512; Mature: 11512

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAIKLEIFIIENVKNLILYTKGYFLEEIKERLNALGYQLSYQILNAKDYGVPQSRERAF
CCEEEEEEEEECCCCEEEEEECCHHHHHHHHHHHHHCEEEEEEEECCCCCCCCCCCCCEE
IVGATHFSFDFNLLEPSQSVSVQEAISDLAYFHSNEGAFGV
EEEEEEEEEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MKAIKLEIFIIENVKNLILYTKGYFLEEIKERLNALGYQLSYQILNAKDYGVPQSRERAF
CCEEEEEEEEECCCCEEEEEECCHHHHHHHHHHHHHCEEEEEEEECCCCCCCCCCCCCEE
IVGATHFSFDFNLLEPSQSVSVQEAISDLAYFHSNEGAFGV
EEEEEEEEEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6313947; 8065896 [H]