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The map label for this gene is dpnA fragment 3 [H]

Identifier: 109946717

GI number: 109946717

Start: 86453

End: 86668

Strand: Reverse

Name: dpnA fragment 3 [H]

Synonym: Hac_0085

Alternate gene names: 109946717

Gene position: 86668-86453 (Counterclockwise)

Preceding gene: 109946718

Following gene: 109946716

Centisome position: 5.58

GC content: 39.35

Gene sequence:

>216_bases
GTGAGTAGGACCGAAAGAGTTAAACACCCCACGCAAAAAAGCCTAGCCTTAATGGAAAAAATCATTTCTATCCACACAAA
CCCTAATGATCTCGTGCTAGATCCTTTTATGGGGAGCGGCACCACCGGTTTAACATGCAAGAATTTAAAGCGAAACTTTA
TCGGTACAGAATTAGAAAAAGAATATTTTCAAATCGCTAAAAAGCGTTTAGGCTAG

Upstream 100 bases:

>100_bases
CCTATGGGCGGTTAAAAAGAAAGCTAAATGGGCGTTTAACAAACCCAACAATGAAACCTAATTTACGCCCCTTGATTTTA
AAAAGCCCCATAGCCCCATA

Downstream 100 bases:

>100_bases
TTTTAAAAACGCTATTTGAATGAGATTGTGTTATAGTTGCTTGCAATGATACCTTAATTAGGATTTTTAATGAAAAGAAT
GTTAGCAGAGTTTGAAAAAA

Product: adenine specific DNA methyltransferase fragment 3

Products: NA

Alternate protein names: M.LlaDCHI B; M.LlaDCHIB; Adenine-specific methyltransferase LlaDCHIB; M.LlaII B [H]

Number of amino acids: Translated: 71; Mature: 70

Protein sequence:

>71_residues
MSRTERVKHPTQKSLALMEKIISIHTNPNDLVLDPFMGSGTTGLTCKNLKRNFIGTELEKEYFQIAKKRLG

Sequences:

>Translated_71_residues
MSRTERVKHPTQKSLALMEKIISIHTNPNDLVLDPFMGSGTTGLTCKNLKRNFIGTELEKEYFQIAKKRLG
>Mature_70_residues
SRTERVKHPTQKSLALMEKIISIHTNPNDLVLDPFMGSGTTGLTCKNLKRNFIGTELEKEYFQIAKKRLG

Specific function: This methylase recognizes the double-stranded sequence GATC, causes specific methylation on A-2 on both strands, and protects the DNA from cleavage by the LlaDCHI endonuclease [H]

COG id: COG0863

COG function: function code L; DNA modification methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI87082238, Length=66, Percent_Identity=48.4848484848485, Blast_Score=66, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002941
- InterPro:   IPR002052
- InterPro:   IPR001091 [H]

Pfam domain/function: PF01555 N6_N4_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 8077; Mature: 7945

Theoretical pI: Translated: 10.41; Mature: 10.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRTERVKHPTQKSLALMEKIISIHTNPNDLVLDPFMGSGTTGLTCKNLKRNFIGTELEK
CCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCHHHHHHHHCCCHHHH
EYFQIAKKRLG
HHHHHHHHHCC
>Mature Secondary Structure 
SRTERVKHPTQKSLALMEKIISIHTNPNDLVLDPFMGSGTTGLTCKNLKRNFIGTELEK
CCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCHHHHHHHHCCCHHHH
EYFQIAKKRLG
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7793939 [H]