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The map label for this gene is gcvA [H]

Identifier: 110681090

GI number: 110681090

Start: 3816098

End: 3816985

Strand: Direct

Name: gcvA [H]

Synonym: RD1_3957

Alternate gene names: 110681090

Gene position: 3816098-3816985 (Clockwise)

Preceding gene: 110681088

Following gene: 110681093

Centisome position: 92.33

GC content: 63.4

Gene sequence:

>888_bases
ATGCAACCTGCGCGCCGTTTGCTCCCCTCCATCACCGCGCTGACCTGTTTCGAGGCGGTCGCCCGTTTAGGCAGCGCCAC
GGATGCAGCGGCTGAACTGTCGCTGACCCAAAGCGCGGTGAGCCGACAGATCAAGGCGCTGGAGGGGCAGTTGGGCATCA
CGCTTTTTGAACGGCGCGGACGACAATTGATCCTGACATCGCGCGGTGCGGCATATGTTTCGGATATCCGCGACATCCTG
CAGCGCCTGACGCGCGCCTCCGTTGCAGTGCGTACCGACGCGCGCGCGGGTGCGCTCAACCTGTCGATCCTGCCCGCTTT
TGGCATGCATTGGCTGGCACCCCGGCTGCAGGACTTTGCCCAGACCCACCCGGAGGTCACCGTCAATCTGTCCACCCGTC
TGGCCCCGTTTGATTTTCGCGATACGCAGTTTGATGCCGCCATCCATTTCGGGCGCGAGGACTGGCCGGCGGCGGACTAC
CTGCCGCTCATGCCAGAGACCGTGGTTGCGGTCTGTGCGCCCTCCCTGATTCCAACGCCGTTAAGCGATGCGCGCGGTAT
TCTGTCCCATGACCTGATGCATCTGGAAACCCGGCCACGCGGTTGGGTGCGGTGGTTGCGATCGCTCGATATTGACGCCT
CGCTGCCCCCCGGCATGGTTTTCGATCAGTTTTCCACCATGGCGCAGGCGGCGGTGCACGGCTTAGGGGTCGCGCTGCTG
CCAACCTTTGTCGCGGACCCGTTCCTGAACAGTGGTCAGTTGGTGCTGGCCTCGCCCCACGCCAACCAGAGCATCGGCAG
CTATTATCTCGTGTGGCCTAAAGACCGCGAAGAGAGCCATAGCCTGACGTCTTTTCGCACCTGGCTGCGCGATCAGGCCC
GAGGCTGA

Upstream 100 bases:

>100_bases
CCTTTCGGCGTCATATCGGCATTAGGTTGCGCCCATTATTGCAAGTTACGGGGCAAAAGGCTAACGAGGCTTTGTCCTGT
CATTCATGAGAAAAAATCAT

Downstream 100 bases:

>100_bases
ACCACGGTTGGACGTGCCGGTCATGCCCGCTCGAATACCTCGCCGCGCCATGCCGTTCCTGCGCCACGGATGTGGCTCGT
TTCTGTCGCAATCCACTTTT

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 295; Mature: 295

Protein sequence:

>295_residues
MQPARRLLPSITALTCFEAVARLGSATDAAAELSLTQSAVSRQIKALEGQLGITLFERRGRQLILTSRGAAYVSDIRDIL
QRLTRASVAVRTDARAGALNLSILPAFGMHWLAPRLQDFAQTHPEVTVNLSTRLAPFDFRDTQFDAAIHFGREDWPAADY
LPLMPETVVAVCAPSLIPTPLSDARGILSHDLMHLETRPRGWVRWLRSLDIDASLPPGMVFDQFSTMAQAAVHGLGVALL
PTFVADPFLNSGQLVLASPHANQSIGSYYLVWPKDREESHSLTSFRTWLRDQARG

Sequences:

>Translated_295_residues
MQPARRLLPSITALTCFEAVARLGSATDAAAELSLTQSAVSRQIKALEGQLGITLFERRGRQLILTSRGAAYVSDIRDIL
QRLTRASVAVRTDARAGALNLSILPAFGMHWLAPRLQDFAQTHPEVTVNLSTRLAPFDFRDTQFDAAIHFGREDWPAADY
LPLMPETVVAVCAPSLIPTPLSDARGILSHDLMHLETRPRGWVRWLRSLDIDASLPPGMVFDQFSTMAQAAVHGLGVALL
PTFVADPFLNSGQLVLASPHANQSIGSYYLVWPKDREESHSLTSFRTWLRDQARG
>Mature_295_residues
MQPARRLLPSITALTCFEAVARLGSATDAAAELSLTQSAVSRQIKALEGQLGITLFERRGRQLILTSRGAAYVSDIRDIL
QRLTRASVAVRTDARAGALNLSILPAFGMHWLAPRLQDFAQTHPEVTVNLSTRLAPFDFRDTQFDAAIHFGREDWPAADY
LPLMPETVVAVCAPSLIPTPLSDARGILSHDLMHLETRPRGWVRWLRSLDIDASLPPGMVFDQFSTMAQAAVHGLGVALL
PTFVADPFLNSGQLVLASPHANQSIGSYYLVWPKDREESHSLTSFRTWLRDQARG

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=289, Percent_Identity=36.6782006920415, Blast_Score=179, Evalue=3e-46,
Organism=Escherichia coli, GI1786448, Length=284, Percent_Identity=29.2253521126761, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1788706, Length=319, Percent_Identity=28.8401253918495, Blast_Score=107, Evalue=8e-25,
Organism=Escherichia coli, GI145693193, Length=298, Percent_Identity=29.1946308724832, Blast_Score=104, Evalue=8e-24,
Organism=Escherichia coli, GI1786401, Length=252, Percent_Identity=28.1746031746032, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI157672245, Length=166, Percent_Identity=31.9277108433735, Blast_Score=78, Evalue=8e-16,
Organism=Escherichia coli, GI1787589, Length=293, Percent_Identity=23.8907849829352, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1790262, Length=258, Percent_Identity=25.1937984496124, Blast_Score=62, Evalue=5e-11,
Organism=Escherichia coli, GI1788481, Length=256, Percent_Identity=25.78125, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32491; Mature: 32491

Theoretical pI: Translated: 7.74; Mature: 7.74

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQPARRLLPSITALTCFEAVARLGSATDAAAELSLTQSAVSRQIKALEGQLGITLFERRG
CCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCC
RQLILTSRGAAYVSDIRDILQRLTRASVAVRTDARAGALNLSILPAFGMHWLAPRLQDFA
CEEEEECCCCHHHHHHHHHHHHHHHHHHHEECCCCCCEEEEEEHHHHHHHHHHHHHHHHH
QTHPEVTVNLSTRLAPFDFRDTQFDAAIHFGREDWPAADYLPLMPETVVAVCAPSLIPTP
HCCCCEEEEEECEECCCCCCCCCHHHHEECCCCCCCCCCCCCCCHHHHHHHHCCCCCCCC
LSDARGILSHDLMHLETRPRGWVRWLRSLDIDASLPPGMVFDQFSTMAQAAVHGLGVALL
CHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
PTFVADPFLNSGQLVLASPHANQSIGSYYLVWPKDREESHSLTSFRTWLRDQARG
HHHHHCCCCCCCCEEEECCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MQPARRLLPSITALTCFEAVARLGSATDAAAELSLTQSAVSRQIKALEGQLGITLFERRG
CCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCC
RQLILTSRGAAYVSDIRDILQRLTRASVAVRTDARAGALNLSILPAFGMHWLAPRLQDFA
CEEEEECCCCHHHHHHHHHHHHHHHHHHHEECCCCCCEEEEEEHHHHHHHHHHHHHHHHH
QTHPEVTVNLSTRLAPFDFRDTQFDAAIHFGREDWPAADYLPLMPETVVAVCAPSLIPTP
HCCCCEEEEEECEECCCCCCCCCHHHHEECCCCCCCCCCCCCCCHHHHHHHHCCCCCCCC
LSDARGILSHDLMHLETRPRGWVRWLRSLDIDASLPPGMVFDQFSTMAQAAVHGLGVALL
CHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
PTFVADPFLNSGQLVLASPHANQSIGSYYLVWPKDREESHSLTSFRTWLRDQARG
HHHHHCCCCCCCCEEEECCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]