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The map label for this gene is maiA [H]

Identifier: 110680805

GI number: 110680805

Start: 3515760

End: 3516395

Strand: Direct

Name: maiA [H]

Synonym: RD1_3652

Alternate gene names: 110680805

Gene position: 3515760-3516395 (Clockwise)

Preceding gene: 110680804

Following gene: 110680806

Centisome position: 85.06

GC content: 62.74

Gene sequence:

>636_bases
ATGCTGACGCTCTATTCCTATTGGCGATCCACCACATCCTACCGGGTGCGGGTGGCGCTGAACCTCAAGGGGCTGTCTTA
TCGGACAGTCCCGGTGGACCTTGTGGCGGGCGATCAGCGCAGACCGGATTACGTGGCGTTGAACCCGGTCCAAGGGGTGC
CCACACTGGTGCTGGAGGACGGCACCGCGCTGACGCAATCGCTCGCGATCCTCGACTATCTGGATCACGTCGTGCCTGAC
CCCGCCCTGCTGCCAAGCGATCCCCTGCTTCGGGCCAAGGTGCAGGCGGCGGCGCAGATCATCGCGCTCGACATTCACCC
CGTGAACAACCTGAAGGTCATGAACCGCCTGAAATCCGAACACGGGGCGAGCGCCGATCAAGGCATCGAATGGATGCGCC
ACTGGATGACGCAAGGTTTCCACGCCTATCAGGCGCTGCTGCCCGATGGCCCCGCCTACAGCTTCTCGGACACGCCGCTG
CTTTGCGACATCTGCCTTGTTGCGCAGCTTTATAACGCACACCGCTGGGGCGTGGACATGACGCCCTTTGCCCGCCTGCT
TGAGATCGAAGAACAGGCCCTGAAACTGCCGGCTTTCGATGCCGCACGCCCCGAAAACCAGCCAGACGCCACCTGA

Upstream 100 bases:

>100_bases
AGTTCGCGGGCAAGGAAGCGCCGCTGCAGGACGATTACATCAATTGCTGGGCGGACATCGAAAAGAAATTCGACGGCACG
CCGGGCAAGAAGTAAGCGCC

Downstream 100 bases:

>100_bases
AGGACAAACGATGACACAACTTCTCCGCTCTTGGGTCGAAAGCGCCAATGCGCCCGACACCGACTTCCCGCTCAACAACC
TGCCCTATGGGGCTTTCCGT

Product: maleylacetoacetate isomerase

Products: NA

Alternate protein names: MAAI [H]

Number of amino acids: Translated: 211; Mature: 211

Protein sequence:

>211_residues
MLTLYSYWRSTTSYRVRVALNLKGLSYRTVPVDLVAGDQRRPDYVALNPVQGVPTLVLEDGTALTQSLAILDYLDHVVPD
PALLPSDPLLRAKVQAAAQIIALDIHPVNNLKVMNRLKSEHGASADQGIEWMRHWMTQGFHAYQALLPDGPAYSFSDTPL
LCDICLVAQLYNAHRWGVDMTPFARLLEIEEQALKLPAFDAARPENQPDAT

Sequences:

>Translated_211_residues
MLTLYSYWRSTTSYRVRVALNLKGLSYRTVPVDLVAGDQRRPDYVALNPVQGVPTLVLEDGTALTQSLAILDYLDHVVPD
PALLPSDPLLRAKVQAAAQIIALDIHPVNNLKVMNRLKSEHGASADQGIEWMRHWMTQGFHAYQALLPDGPAYSFSDTPL
LCDICLVAQLYNAHRWGVDMTPFARLLEIEEQALKLPAFDAARPENQPDAT
>Mature_211_residues
MLTLYSYWRSTTSYRVRVALNLKGLSYRTVPVDLVAGDQRRPDYVALNPVQGVPTLVLEDGTALTQSLAILDYLDHVVPD
PALLPSDPLLRAKVQAAAQIIALDIHPVNNLKVMNRLKSEHGASADQGIEWMRHWMTQGFHAYQALLPDGPAYSFSDTPL
LCDICLVAQLYNAHRWGVDMTPFARLLEIEEQALKLPAFDAARPENQPDAT

Specific function: Forms An Equimolar Complex With The RNA Polymerase Holoenzyme (Rnap) But Not With The Core Enzyme. It Is Synthesized Predominantly When Cells Are Exposed To Amino Acid Starvation, At Which Time It Accounts For Over 50% Of The Total Protein Synthesized. It

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GST N-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI22202624, Length=209, Percent_Identity=41.6267942583732, Blast_Score=160, Evalue=6e-40,
Organism=Homo sapiens, GI22202622, Length=162, Percent_Identity=37.037037037037, Blast_Score=106, Evalue=1e-23,
Organism=Homo sapiens, GI194440732, Length=209, Percent_Identity=32.5358851674641, Blast_Score=105, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI17551302, Length=208, Percent_Identity=37.5, Blast_Score=142, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI17510461, Length=209, Percent_Identity=36.8421052631579, Blast_Score=132, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17556142, Length=209, Percent_Identity=38.2775119617225, Blast_Score=129, Evalue=9e-31,
Organism=Drosophila melanogaster, GI24645375, Length=210, Percent_Identity=40.9523809523809, Blast_Score=157, Evalue=4e-39,
Organism=Drosophila melanogaster, GI21355859, Length=210, Percent_Identity=40.9523809523809, Blast_Score=157, Evalue=4e-39,
Organism=Drosophila melanogaster, GI45553325, Length=210, Percent_Identity=40.9523809523809, Blast_Score=157, Evalue=4e-39,
Organism=Drosophila melanogaster, GI21355857, Length=211, Percent_Identity=36.9668246445498, Blast_Score=152, Evalue=1e-37,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1982 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010987
- InterPro:   IPR004045
- InterPro:   IPR017933
- InterPro:   IPR004046
- InterPro:   IPR005955
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00043 GST_C; PF02798 GST_N [H]

EC number: =5.2.1.2 [H]

Molecular weight: Translated: 23503; Mature: 23503

Theoretical pI: Translated: 5.55; Mature: 5.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTLYSYWRSTTSYRVRVALNLKGLSYRTVPVDLVAGDQRRPDYVALNPVQGVPTLVLED
CEEEEHHHCCCCCEEEEEEEEECCCEEEEEEEEEEECCCCCCCEEEECCCCCCCEEEEEC
GTALTQSLAILDYLDHVVPDPALLPSDPLLRAKVQAAAQIIALDIHPVNNLKVMNRLKSE
CHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHEEEEEEECCCCHHHHHHHHHHH
HGASADQGIEWMRHWMTQGFHAYQALLPDGPAYSFSDTPLLCDICLVAQLYNAHRWGVDM
CCCCHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCH
TPFARLLEIEEQALKLPAFDAARPENQPDAT
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MLTLYSYWRSTTSYRVRVALNLKGLSYRTVPVDLVAGDQRRPDYVALNPVQGVPTLVLED
CEEEEHHHCCCCCEEEEEEEEECCCEEEEEEEEEEECCCCCCCEEEECCCCCCCEEEEEC
GTALTQSLAILDYLDHVVPDPALLPSDPLLRAKVQAAAQIIALDIHPVNNLKVMNRLKSE
CHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHEEEEEEECCCCHHHHHHHHHHH
HGASADQGIEWMRHWMTQGFHAYQALLPDGPAYSFSDTPLLCDICLVAQLYNAHRWGVDM
CCCCHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCH
TPFARLLEIEEQALKLPAFDAARPENQPDAT
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]