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The map label for this gene is thiD [H]

Identifier: 110680769

GI number: 110680769

Start: 3475220

End: 3476029

Strand: Direct

Name: thiD [H]

Synonym: RD1_3612

Alternate gene names: 110680769

Gene position: 3475220-3476029 (Clockwise)

Preceding gene: 110680768

Following gene: 110680770

Centisome position: 84.08

GC content: 61.73

Gene sequence:

>810_bases
ATGATACCCAACGTCCTCAGTATCGCCGGGTCTGACCCTTCCGGAGGCGCAGGCATTCAGGCCGACATCAAGGCCATTTC
CGCCAATGGCGCCTTTGCCATGAACGCCATCACAGCTTTGACCGCACAGAACACGCAAGGCGTGAGCGGCATTCACCTGA
TCCCGCCCGCCTTTGTGCAGGCCCAGATCAAAGCCATCTTCGACGACATCCGTGTGGATGCGGTGAAGATCGGCATGATC
GCCAATGCCGTGATCGCAGCCGCTGTGGCAGATGCGTTGCAGGGCCACGGCGATATTCCCATCGTGCTTGACCCGGTGAT
GATTGCCAAAGGGGGCGCGGCCTTGTTGGAACCGGAGGCGGTCGATACCCTGCGCTGCGCGCTTTTGCCGATCGCGACCG
TGTTGACACCCAACCTGCCCGAAGCGGCACATCTGCTTGGCACCGACATCGCCAAAACACGCGATGACATGGCCAAACAA
GGTGCCGCCCTTTGCGCGCTTGGCCCCAAAGCGACGCTGATGAAAGGCGGGCATCTTGAGGGGTCTGAAAGCCCCGATTG
CCTTGTGACCGCCGAGACCGTGACATGGTTTGATGCACAGCGCACCGCCACGCAAAACACCCATGGCACCGGCTGTACCC
TGTCCTCGGCCCTGGCTGCGCAACTGGCCAAAGGACACCCCCCGGCCAAAGCTGCGTCCGCAGCCAAATCCTATGTCGCA
CAGGCCATCGCGGCGGCAGACGCCTTGTCTGTCGGGACCGGCCACGGCCCCACGCACCATTTGGCATCGTTTTATTCCCA
AAGGACGTGA

Upstream 100 bases:

>100_bases
GCGGTGCCGATGGCATGGCCGTCGTGTCCGCCATCTGTGGTCAGCCCGATCCACAGGCGGCGGCTGGCGCTTTTCATAGT
TTCAAACCGGAGCATAAAGC

Downstream 100 bases:

>100_bases
AGGGTTTCTGACCCCCCGTCTTTGTTGAACACACCCGACAAAAGGACCATCACATGACCAAATCCATCTATGGCGAGACT
TTCGCGCTTTGGCGTGACGG

Product: phosphomethylpyrimidine kinase

Products: NA

Alternate protein names: Hydroxymethylpyrimidine kinase; HMP kinase; Hydroxymethylpyrimidine phosphate kinase; HMP-P kinase; HMP-phosphate kinase; HMPP kinase [H]

Number of amino acids: Translated: 269; Mature: 269

Protein sequence:

>269_residues
MIPNVLSIAGSDPSGGAGIQADIKAISANGAFAMNAITALTAQNTQGVSGIHLIPPAFVQAQIKAIFDDIRVDAVKIGMI
ANAVIAAAVADALQGHGDIPIVLDPVMIAKGGAALLEPEAVDTLRCALLPIATVLTPNLPEAAHLLGTDIAKTRDDMAKQ
GAALCALGPKATLMKGGHLEGSESPDCLVTAETVTWFDAQRTATQNTHGTGCTLSSALAAQLAKGHPPAKAASAAKSYVA
QAIAAADALSVGTGHGPTHHLASFYSQRT

Sequences:

>Translated_269_residues
MIPNVLSIAGSDPSGGAGIQADIKAISANGAFAMNAITALTAQNTQGVSGIHLIPPAFVQAQIKAIFDDIRVDAVKIGMI
ANAVIAAAVADALQGHGDIPIVLDPVMIAKGGAALLEPEAVDTLRCALLPIATVLTPNLPEAAHLLGTDIAKTRDDMAKQ
GAALCALGPKATLMKGGHLEGSESPDCLVTAETVTWFDAQRTATQNTHGTGCTLSSALAAQLAKGHPPAKAASAAKSYVA
QAIAAADALSVGTGHGPTHHLASFYSQRT
>Mature_269_residues
MIPNVLSIAGSDPSGGAGIQADIKAISANGAFAMNAITALTAQNTQGVSGIHLIPPAFVQAQIKAIFDDIRVDAVKIGMI
ANAVIAAAVADALQGHGDIPIVLDPVMIAKGGAALLEPEAVDTLRCALLPIATVLTPNLPEAAHLLGTDIAKTRDDMAKQ
GAALCALGPKATLMKGGHLEGSESPDCLVTAETVTWFDAQRTATQNTHGTGCTLSSALAAQLAKGHPPAKAASAAKSYVA
QAIAAADALSVGTGHGPTHHLASFYSQRT

Specific function: Catalyzes the phosphorylation of hydroxymethylpyrimidine phosphate (HMP-P) to HMP-PP, and of HMP to HMP-P. Shows no activity with pyridoxal, pyridoxamine or pyridoxine [H]

COG id: COG0351

COG function: function code H; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiD family [H]

Homologues:

Organism=Escherichia coli, GI1788420, Length=262, Percent_Identity=52.2900763358779, Blast_Score=261, Evalue=4e-71,
Organism=Saccharomyces cerevisiae, GI6325378, Length=273, Percent_Identity=33.3333333333333, Blast_Score=136, Evalue=3e-33,
Organism=Saccharomyces cerevisiae, GI6324997, Length=274, Percent_Identity=36.8613138686131, Blast_Score=132, Evalue=6e-32,
Organism=Saccharomyces cerevisiae, GI6324517, Length=278, Percent_Identity=34.5323741007194, Blast_Score=127, Evalue=2e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013749
- InterPro:   IPR004399 [H]

Pfam domain/function: PF08543 Phos_pyr_kin [H]

EC number: =2.7.1.49; =2.7.4.7 [H]

Molecular weight: Translated: 27100; Mature: 27100

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPNVLSIAGSDPSGGAGIQADIKAISANGAFAMNAITALTAQNTQGVSGIHLIPPAFVQ
CCCCEEEECCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHCCCCCCCCCEEECCHHHHH
AQIKAIFDDIRVDAVKIGMIANAVIAAAVADALQGHGDIPIVLDPVMIAKGGAALLEPEA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCEEEECCCCCEECCHH
VDTLRCALLPIATVLTPNLPEAAHLLGTDIAKTRDDMAKQGAALCALGPKATLMKGGHLE
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHEEECCCCC
GSESPDCLVTAETVTWFDAQRTATQNTHGTGCTLSSALAAQLAKGHPPAKAASAAKSYVA
CCCCCCEEEEEEEEEEECCHHHCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHH
QAIAAADALSVGTGHGPTHHLASFYSQRT
HHHHHHHHHHCCCCCCCHHHHHHHHHCCC
>Mature Secondary Structure
MIPNVLSIAGSDPSGGAGIQADIKAISANGAFAMNAITALTAQNTQGVSGIHLIPPAFVQ
CCCCEEEECCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHCCCCCCCCCEEECCHHHHH
AQIKAIFDDIRVDAVKIGMIANAVIAAAVADALQGHGDIPIVLDPVMIAKGGAALLEPEA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCEEEECCCCCEECCHH
VDTLRCALLPIATVLTPNLPEAAHLLGTDIAKTRDDMAKQGAALCALGPKATLMKGGHLE
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHEEECCCCC
GSESPDCLVTAETVTWFDAQRTATQNTHGTGCTLSSALAAQLAKGHPPAKAASAAKSYVA
CCCCCCEEEEEEEEEEECCHHHCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHH
QAIAAADALSVGTGHGPTHHLASFYSQRT
HHHHHHHHHHCCCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10075431; 9097040; 9278503 [H]