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The map label for this gene is pyrB [H]

Identifier: 110680597

GI number: 110680597

Start: 3292065

End: 3293063

Strand: Direct

Name: pyrB [H]

Synonym: RD1_3427

Alternate gene names: 110680597

Gene position: 3292065-3293063 (Clockwise)

Preceding gene: 110680591

Following gene: 110680598

Centisome position: 79.65

GC content: 58.16

Gene sequence:

>999_bases
TTGCCGATGGCACAACCCCTGCCTATAAGGTGGAAAATCGCAATAGGTTGGCCCATGACATTTCCGCACCGACACCTGCT
TGGTATCGAGGCTCTTCGCCCGGAAGAGATCAATACTCTGCTTGATCTGGCGGACAAATACGCAGCGCTGAACCGACAGC
CCGACAAACACGCCGATGCCCTCAAGGGTCTGACGCAAATCAACATGTTCTTCGAAAATTCGACCCGCACGCAGGCGAGT
TTCGAGATCGCGGGCAAGCGGCTTGGCGCGGATGTGATGAACATGGCGATGCAGGCCAGTTCGGTCAAAAAGGGCGAAAC
GCTGATTGACACCGCGCTCACCCTGAATGCGATGCACCCTGATTTGCTGGTGGTGCGGCACCCGCAATCGGGTGCTGTGG
ATCTCTTGGCGCAAAAAGTGAACTGTGCCGTACTGAACGCGGGCGATGGGCGCCATGAACACCCGACGCAGGCCCTGCTG
GATGCGCTGACGATCCGGCGTGCCAAGGGGCGCTTGCATCGCCTGTCCATCGCGATCTGTGGCGATATTGCGCACAGCCG
CGTGGCGCGCTCCAACATCATGCTGCTGGGCAAGATGGAAAACCGCATACGTCTGATCGGCCCGCCGACCCTGATGCCCA
GCGGTATTTCCGAGTTCGGCGTCGAGGTGTTCAACGATATGAACGCGGGCCTGAAAGACGTCGATGTGGTGATGATGCTG
CGTCTGCAAAAAGAACGTATGGATGGCGGGTTCATCCCCTCTGAACGTGAATACTACCACCGCTTCGGGCTGGATGCGGA
TAAGCTGTCCAACGCCAAACCGGATGCGATCATCATGCACCCCGGCCCCATGAACAGGGGCGTTGAGATTGACGGGACGC
TGGCCGATGACATCAACCGCAGCGTCATCCAGGATCAGGTCGAAATGGGTGTCGCAGTGCGCATGGCCGCGATGGATCTG
CTGTCTCAAAACCTAAGACTAACGCGAGGTGCTGAATGA

Upstream 100 bases:

>100_bases
GCAAAGCCAACGCATGATGAAAGTCGGATGATTCCATGAGCGTATTCTACGCGCCCCATCACGCGAACTAAACAGCCAGT
TGCACATTGGCGTCAGCACC

Downstream 100 bases:

>100_bases
CCACCCTATCAATCCCGGCACTGGAAAGCGGCGTGATCCGGGTTTTTGCCCTGTCGATGTCGGATCAGGATGCCAAGGCC
CTGCGCGATGGTGCCGGATC

Product: aspartate carbamoyltransferase catalytic subunit

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase [H]

Number of amino acids: Translated: 332; Mature: 331

Protein sequence:

>332_residues
MPMAQPLPIRWKIAIGWPMTFPHRHLLGIEALRPEEINTLLDLADKYAALNRQPDKHADALKGLTQINMFFENSTRTQAS
FEIAGKRLGADVMNMAMQASSVKKGETLIDTALTLNAMHPDLLVVRHPQSGAVDLLAQKVNCAVLNAGDGRHEHPTQALL
DALTIRRAKGRLHRLSIAICGDIAHSRVARSNIMLLGKMENRIRLIGPPTLMPSGISEFGVEVFNDMNAGLKDVDVVMML
RLQKERMDGGFIPSEREYYHRFGLDADKLSNAKPDAIIMHPGPMNRGVEIDGTLADDINRSVIQDQVEMGVAVRMAAMDL
LSQNLRLTRGAE

Sequences:

>Translated_332_residues
MPMAQPLPIRWKIAIGWPMTFPHRHLLGIEALRPEEINTLLDLADKYAALNRQPDKHADALKGLTQINMFFENSTRTQAS
FEIAGKRLGADVMNMAMQASSVKKGETLIDTALTLNAMHPDLLVVRHPQSGAVDLLAQKVNCAVLNAGDGRHEHPTQALL
DALTIRRAKGRLHRLSIAICGDIAHSRVARSNIMLLGKMENRIRLIGPPTLMPSGISEFGVEVFNDMNAGLKDVDVVMML
RLQKERMDGGFIPSEREYYHRFGLDADKLSNAKPDAIIMHPGPMNRGVEIDGTLADDINRSVIQDQVEMGVAVRMAAMDL
LSQNLRLTRGAE
>Mature_331_residues
PMAQPLPIRWKIAIGWPMTFPHRHLLGIEALRPEEINTLLDLADKYAALNRQPDKHADALKGLTQINMFFENSTRTQASF
EIAGKRLGADVMNMAMQASSVKKGETLIDTALTLNAMHPDLLVVRHPQSGAVDLLAQKVNCAVLNAGDGRHEHPTQALLD
ALTIRRAKGRLHRLSIAICGDIAHSRVARSNIMLLGKMENRIRLIGPPTLMPSGISEFGVEVFNDMNAGLKDVDVVMMLR
LQKERMDGGFIPSEREYYHRFGLDADKLSNAKPDAIIMHPGPMNRGVEIDGTLADDINRSVIQDQVEMGVAVRMAAMDLL
SQNLRLTRGAE

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=305, Percent_Identity=35.7377049180328, Blast_Score=177, Evalue=9e-45,
Organism=Escherichia coli, GI2367364, Length=311, Percent_Identity=37.2990353697749, Blast_Score=151, Evalue=5e-38,
Organism=Escherichia coli, GI1790703, Length=195, Percent_Identity=30.7692307692308, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1786469, Length=227, Percent_Identity=27.3127753303965, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI193204318, Length=342, Percent_Identity=34.5029239766082, Blast_Score=141, Evalue=6e-34,
Organism=Saccharomyces cerevisiae, GI6322331, Length=313, Percent_Identity=35.1437699680511, Blast_Score=151, Evalue=1e-37,
Organism=Drosophila melanogaster, GI24642586, Length=306, Percent_Identity=34.640522875817, Blast_Score=144, Evalue=6e-35,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082 [H]

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N [H]

EC number: =2.1.3.2 [H]

Molecular weight: Translated: 36716; Mature: 36585

Theoretical pI: Translated: 7.82; Mature: 7.82

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
6.0 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
5.7 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPMAQPLPIRWKIAIGWPMTFPHRHLLGIEALRPEEINTLLDLADKYAALNRQPDKHADA
CCCCCCCCEEEEEEEECCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHH
LKGLTQINMFFENSTRTQASFEIAGKRLGADVMNMAMQASSVKKGETLIDTALTLNAMHP
HHHHHHEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHEEECCCC
DLLVVRHPQSGAVDLLAQKVNCAVLNAGDGRHEHPTQALLDALTIRRAKGRLHRLSIAIC
CEEEEECCCCCHHHHHHHHCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCCEEEEEEEEE
GDIAHSRVARSNIMLLGKMENRIRLIGPPTLMPSGISEFGVEVFNDMNAGLKDVDVVMML
HHHHHHHHHHCCEEEEEECCCCEEEEECCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHH
RLQKERMDGGFIPSEREYYHRFGLDADKLSNAKPDAIIMHPGPMNRGVEIDGTLADDINR
HHHHHHCCCCCCCCHHHHHHHHCCCHHHHCCCCCCEEEECCCCCCCCEEECCCCHHHHHH
SVIQDQVEMGVAVRMAAMDLLSQNLRLTRGAE
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
PMAQPLPIRWKIAIGWPMTFPHRHLLGIEALRPEEINTLLDLADKYAALNRQPDKHADA
CCCCCCCEEEEEEEECCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHH
LKGLTQINMFFENSTRTQASFEIAGKRLGADVMNMAMQASSVKKGETLIDTALTLNAMHP
HHHHHHEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHEEECCCC
DLLVVRHPQSGAVDLLAQKVNCAVLNAGDGRHEHPTQALLDALTIRRAKGRLHRLSIAIC
CEEEEECCCCCHHHHHHHHCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCCEEEEEEEEE
GDIAHSRVARSNIMLLGKMENRIRLIGPPTLMPSGISEFGVEVFNDMNAGLKDVDVVMML
HHHHHHHHHHCCEEEEEECCCCEEEEECCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHH
RLQKERMDGGFIPSEREYYHRFGLDADKLSNAKPDAIIMHPGPMNRGVEIDGTLADDINR
HHHHHHCCCCCCCCHHHHHHHHCCCHHHHCCCCCCEEEECCCCCCCCEEECCCCHHHHHH
SVIQDQVEMGVAVRMAAMDLLSQNLRLTRGAE
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA