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The map label for this gene is dnaB [H]

Identifier: 110680418

GI number: 110680418

Start: 3106588

End: 3108081

Strand: Direct

Name: dnaB [H]

Synonym: RD1_3237

Alternate gene names: 110680418

Gene position: 3106588-3108081 (Clockwise)

Preceding gene: 110680417

Following gene: 110680419

Centisome position: 75.16

GC content: 59.3

Gene sequence:

>1494_bases
ATGAACGAGATATCGACCTTCAATCCGACCGGCGTTGATGTGCAATCGCCTGAGACCATGCCCCATTCCATCGAGGCGGA
ACAGCAGTTGCTCGGTGCCATCCTGACCAACAACGACATCTACGACCGCGTGGCTTCGATCATTGGACCCCAGCATTTTT
ATGACCCCGTCCATGCGCGCATTTTCGAGATTGCCGCCGCGCGGATTGCCAAGAACAGCCTGGCCTCCCCGGTCACGCTC
AAAGCCTTCATGGAGGACGACGAGGGCCTCAAGGAGCTTGGCGGCCCGGCGTATCTGGCGCGCCTTGCGGGGGCGGCGAT
TTCTGCATTTGCGGTCAAGGACTACGCGCAGATGATCTATGATCTGGCGGTGCGGCGGGACCTGATCCAGCTTGGGCGCA
CGATATCGGACAAGGCGGCGCATGTGGATGTGGCCTCTGAACCGCGCGAGCAGATTGTCGAGGCGGAACAGGCGTTATAC
GCGCTTGCGGAACAGGGGCAGTCCGAAAGTGGTTTTCAAAGCTTTCTCAAGGCAGTAACGGATGCTGTTAATGTTGCAAA
TGCCGCCTATCAGCGCGACGGCGGCATGTCAGGTGTCTCCACCGGCCTGATCGACATGGACAAGAAGCTTGGCGGTTTGC
ACCGGTCGGATTTGCTGATCCTCGCGGGTCGTCCATCCATGGGCAAGACCTCTCTTGCGACGAACATCGCCTTCAACATC
GCCAAGGCCTACAAGCGCGGCGTGTTGCCCGACGGCAGCGAAGGTGCCGTTGATGGTGGTGTGGTCGGGTTCTACTCCCT
TGAGATGAGCGCGGAACAACTCGCCGCGCGGATTTTGTCTGAAGCCTCCGAAGTGCCCTCGGAACAGATCCGGCGGGGCG
ATATGACAGAAACCGAATTCCGACGCTTCGTGGATGCGGCGAAATCGCTGGAGGCCTGCCCGCTTTACATAGACGACACA
CCCGCGCTGCCGATCGCGCAGCTTGCGGCCCGCGCGCGGCGGCTCAAACGGACCCACGGGCTTGATGTGCTGATGGTGGA
TTATCTGCAACTGGTGCGCGGGACGGGTAAATCCGAGAACCGCGTGAATGAAATCTCCGAGATTACAATGGGCCTCAAGG
CCATCGCCAAAGAGCTGGATATCCCGGTCGTTGCCCTGTCGCAGTTGTCACGTCAGGTGGAAAGCCGCGAAGACAAACGC
CCCCAGCTCAGCGATCTGCGCGAATCAGGCTCCATCGAACAGGATGCCGATGTGGTCATGTTCGTGTTCCGCGAAGAATA
TTACAAGGAACGCGAAAAACCGGGCGACCACGACCTTGAAGCCATGGCAAAATGGCAGGAAGAGATGGATCGCCTGCATG
GCCGCGCCGAAGTGGTTATCGGCAAGCAGCGACATGGCCCTATCGGTACGGTTGAACTCAGTTTCGAGGGGCGTTTCACG
CGCTTTGGCAATCTGGTGCAGCCCTGGCAACAGGGCAGCGACCGCGAATTCTGA

Upstream 100 bases:

>100_bases
AGCTTTGCCCCGATAGGCGGCAGGGGAATTTTCGACTACATCTGTCACATAAGCGTTACAAAAATCAGCAGCAGCAAATG
CCGCGCAGGCAAGGACAGTC

Downstream 100 bases:

>100_bases
TTGCCCAACGGCTCAAACAGAACGGGACCCCGTCGGTGCGGTTGCCTTTTGTGCAAACGCCTCCCTTTCAGCGCGTTCCG
CGTCCTGTAAGGGCGGATGC

Product: replicative DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 497; Mature: 497

Protein sequence:

>497_residues
MNEISTFNPTGVDVQSPETMPHSIEAEQQLLGAILTNNDIYDRVASIIGPQHFYDPVHARIFEIAAARIAKNSLASPVTL
KAFMEDDEGLKELGGPAYLARLAGAAISAFAVKDYAQMIYDLAVRRDLIQLGRTISDKAAHVDVASEPREQIVEAEQALY
ALAEQGQSESGFQSFLKAVTDAVNVANAAYQRDGGMSGVSTGLIDMDKKLGGLHRSDLLILAGRPSMGKTSLATNIAFNI
AKAYKRGVLPDGSEGAVDGGVVGFYSLEMSAEQLAARILSEASEVPSEQIRRGDMTETEFRRFVDAAKSLEACPLYIDDT
PALPIAQLAARARRLKRTHGLDVLMVDYLQLVRGTGKSENRVNEISEITMGLKAIAKELDIPVVALSQLSRQVESREDKR
PQLSDLRESGSIEQDADVVMFVFREEYYKEREKPGDHDLEAMAKWQEEMDRLHGRAEVVIGKQRHGPIGTVELSFEGRFT
RFGNLVQPWQQGSDREF

Sequences:

>Translated_497_residues
MNEISTFNPTGVDVQSPETMPHSIEAEQQLLGAILTNNDIYDRVASIIGPQHFYDPVHARIFEIAAARIAKNSLASPVTL
KAFMEDDEGLKELGGPAYLARLAGAAISAFAVKDYAQMIYDLAVRRDLIQLGRTISDKAAHVDVASEPREQIVEAEQALY
ALAEQGQSESGFQSFLKAVTDAVNVANAAYQRDGGMSGVSTGLIDMDKKLGGLHRSDLLILAGRPSMGKTSLATNIAFNI
AKAYKRGVLPDGSEGAVDGGVVGFYSLEMSAEQLAARILSEASEVPSEQIRRGDMTETEFRRFVDAAKSLEACPLYIDDT
PALPIAQLAARARRLKRTHGLDVLMVDYLQLVRGTGKSENRVNEISEITMGLKAIAKELDIPVVALSQLSRQVESREDKR
PQLSDLRESGSIEQDADVVMFVFREEYYKEREKPGDHDLEAMAKWQEEMDRLHGRAEVVIGKQRHGPIGTVELSFEGRFT
RFGNLVQPWQQGSDREF
>Mature_497_residues
MNEISTFNPTGVDVQSPETMPHSIEAEQQLLGAILTNNDIYDRVASIIGPQHFYDPVHARIFEIAAARIAKNSLASPVTL
KAFMEDDEGLKELGGPAYLARLAGAAISAFAVKDYAQMIYDLAVRRDLIQLGRTISDKAAHVDVASEPREQIVEAEQALY
ALAEQGQSESGFQSFLKAVTDAVNVANAAYQRDGGMSGVSTGLIDMDKKLGGLHRSDLLILAGRPSMGKTSLATNIAFNI
AKAYKRGVLPDGSEGAVDGGVVGFYSLEMSAEQLAARILSEASEVPSEQIRRGDMTETEFRRFVDAAKSLEACPLYIDDT
PALPIAQLAARARRLKRTHGLDVLMVDYLQLVRGTGKSENRVNEISEITMGLKAIAKELDIPVVALSQLSRQVESREDKR
PQLSDLRESGSIEQDADVVMFVFREEYYKEREKPGDHDLEAMAKWQEEMDRLHGRAEVVIGKQRHGPIGTVELSFEGRFT
RFGNLVQPWQQGSDREF

Specific function: Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity and contains distinct active sites for ATP binding, DNA binding, and interaction with dnaC protein, primase, and other prepriming proteins [

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=473, Percent_Identity=39.323467230444, Blast_Score=296, Evalue=1e-81,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 54658; Mature: 54658

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS50162 RECA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEISTFNPTGVDVQSPETMPHSIEAEQQLLGAILTNNDIYDRVASIIGPQHFYDPVHAR
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHCCHHHHH
IFEIAAARIAKNSLASPVTLKAFMEDDEGLKELGGPAYLARLAGAAISAFAVKDYAQMIY
HHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
DLAVRRDLIQLGRTISDKAAHVDVASEPREQIVEAEQALYALAEQGQSESGFQSFLKAVT
HHHHHHHHHHHCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
DAVNVANAAYQRDGGMSGVSTGLIDMDKKLGGLHRSDLLILAGRPSMGKTSLATNIAFNI
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHHH
AKAYKRGVLPDGSEGAVDGGVVGFYSLEMSAEQLAARILSEASEVPSEQIRRGDMTETEF
HHHHHCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCHHHH
RRFVDAAKSLEACPLYIDDTPALPIAQLAARARRLKRTHGLDVLMVDYLQLVRGTGKSEN
HHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCH
RVNEISEITMGLKAIAKELDIPVVALSQLSRQVESREDKRPQLSDLRESGSIEQDADVVM
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCHHHH
FVFREEYYKEREKPGDHDLEAMAKWQEEMDRLHGRAEVVIGKQRHGPIGTVELSFEGRFT
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCEEEEEEECCCHH
RFGNLVQPWQQGSDREF
HHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MNEISTFNPTGVDVQSPETMPHSIEAEQQLLGAILTNNDIYDRVASIIGPQHFYDPVHAR
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHCCHHHHH
IFEIAAARIAKNSLASPVTLKAFMEDDEGLKELGGPAYLARLAGAAISAFAVKDYAQMIY
HHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
DLAVRRDLIQLGRTISDKAAHVDVASEPREQIVEAEQALYALAEQGQSESGFQSFLKAVT
HHHHHHHHHHHCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
DAVNVANAAYQRDGGMSGVSTGLIDMDKKLGGLHRSDLLILAGRPSMGKTSLATNIAFNI
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHHH
AKAYKRGVLPDGSEGAVDGGVVGFYSLEMSAEQLAARILSEASEVPSEQIRRGDMTETEF
HHHHHCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCHHHH
RRFVDAAKSLEACPLYIDDTPALPIAQLAARARRLKRTHGLDVLMVDYLQLVRGTGKSEN
HHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCH
RVNEISEITMGLKAIAKELDIPVVALSQLSRQVESREDKRPQLSDLRESGSIEQDADVVM
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCHHHH
FVFREEYYKEREKPGDHDLEAMAKWQEEMDRLHGRAEVVIGKQRHGPIGTVELSFEGRFT
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCEEEEEEECCCHH
RFGNLVQPWQQGSDREF
HHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA