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The map label for this gene is fabI-2 [H]

Identifier: 110680382

GI number: 110680382

Start: 3064112

End: 3064987

Strand: Direct

Name: fabI-2 [H]

Synonym: RD1_3199

Alternate gene names: 110680382

Gene position: 3064112-3064987 (Clockwise)

Preceding gene: 110680373

Following gene: 110680383

Centisome position: 74.14

GC content: 57.65

Gene sequence:

>876_bases
ATGGCCATCGCGCAATCGCCTAAAGACCAAGGACAAGAGAGCAACCATTCAGTTACCAAGGGAAATTTCATGGGAAACGA
GCTTCTGGCGGGAAAACGCGGCCTGATCATGGGCCTTGCAAATGACAAGTCCATCGCCTGGGGTATTGCCAAGACCTGCG
CCGATGCCGGGGCGGAACTTGCCTTTTCCTATCAGGGCGAGGCGCTGAAAAAGCGGGTCGGGCCGCTGGCGGAAAAGCTG
GGCAGCGATATCGTCCTGCCCTGCGATGTAAGCGATGAGGCCTCGATGGATGCGCTGTTTGACGCGCTCAAGGACAAATG
GGGCACGCTGGACTTCATCGTCCATGCCATCGGGTTCTCCGACAAGGGCGAATTGCGCGGACGCTATGTGGACACCACGC
GCGACAATTTCGCGATGACGATGGATATTTCGGTCTATTCCTTTACCTCGGTCGTGCAACGGGCGGAAAAGATGATGAAC
CCGGGCGGGTCCTGCCTGACAATGACCTATTATGGTGCCGAAAAGGTCATGCCGCATTATAACGTGATGGGTGTGGCCAA
GGCGGCGCTTGAGGCATCGGTGCAGTATCTGGCCGAAGACCTTGGCAAGGATGGCATCCGCGTGAATGCGATCTCCGCCG
GGCCGATCAAGACGCTGGCCGCGAGCGGGATTGGCGATTTCCGTTATATCATGAAGTGGAACGAGTATAACTCACCCCTG
CGGCGCAATGTGACGATTGATGACGTGGGCAAGGCGGCCCTGTTCCTGTTGTCTGACCTCGGTTCAGGCACCACGGGGGA
GAACCTGCATGTGGATGCGGGGTATCATGTGGTGGGCATGAAGGCGGTGGACGCCCCCGACATGAGCAAGGAATAA

Upstream 100 bases:

>100_bases
TCATGATGCACCTTTCGCGTTATCTCGTCCGTGGCACAGGTGGCCGAAACACGCCAAAGGTCAAGAGGGGCCGCGCAAGT
TGGGGCCGCGAGCAGCCTTG

Downstream 100 bases:

>100_bases
CGCAGTGAGCGATCTGTCCTGGGCCCAGCTTGCCGCTTTCAACGTCACCCTGTTGGTGGCGATGGCGGCACCGGGTCCGG
CGTTTCTGCTGGTGTTGCGC

Product: enoyl-(acyl carrier protein) reductase

Products: NA

Alternate protein names: NADH-dependent enoyl-ACP reductase 1 [H]

Number of amino acids: Translated: 291; Mature: 290

Protein sequence:

>291_residues
MAIAQSPKDQGQESNHSVTKGNFMGNELLAGKRGLIMGLANDKSIAWGIAKTCADAGAELAFSYQGEALKKRVGPLAEKL
GSDIVLPCDVSDEASMDALFDALKDKWGTLDFIVHAIGFSDKGELRGRYVDTTRDNFAMTMDISVYSFTSVVQRAEKMMN
PGGSCLTMTYYGAEKVMPHYNVMGVAKAALEASVQYLAEDLGKDGIRVNAISAGPIKTLAASGIGDFRYIMKWNEYNSPL
RRNVTIDDVGKAALFLLSDLGSGTTGENLHVDAGYHVVGMKAVDAPDMSKE

Sequences:

>Translated_291_residues
MAIAQSPKDQGQESNHSVTKGNFMGNELLAGKRGLIMGLANDKSIAWGIAKTCADAGAELAFSYQGEALKKRVGPLAEKL
GSDIVLPCDVSDEASMDALFDALKDKWGTLDFIVHAIGFSDKGELRGRYVDTTRDNFAMTMDISVYSFTSVVQRAEKMMN
PGGSCLTMTYYGAEKVMPHYNVMGVAKAALEASVQYLAEDLGKDGIRVNAISAGPIKTLAASGIGDFRYIMKWNEYNSPL
RRNVTIDDVGKAALFLLSDLGSGTTGENLHVDAGYHVVGMKAVDAPDMSKE
>Mature_290_residues
AIAQSPKDQGQESNHSVTKGNFMGNELLAGKRGLIMGLANDKSIAWGIAKTCADAGAELAFSYQGEALKKRVGPLAEKLG
SDIVLPCDVSDEASMDALFDALKDKWGTLDFIVHAIGFSDKGELRGRYVDTTRDNFAMTMDISVYSFTSVVQRAEKMMNP
GGSCLTMTYYGAEKVMPHYNVMGVAKAALEASVQYLAEDLGKDGIRVNAISAGPIKTLAASGIGDFRYIMKWNEYNSPLR
RNVTIDDVGKAALFLLSDLGSGTTGENLHVDAGYHVVGMKAVDAPDMSKE

Specific function: Fatty acid biosynthesis pathway; second reduction step. [C]

COG id: COG0623

COG function: function code I; Enoyl-[acyl-carrier-protein] reductase (NADH)

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family. FabI subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787545, Length=258, Percent_Identity=51.1627906976744, Blast_Score=270, Evalue=6e-74,
Organism=Escherichia coli, GI1787905, Length=252, Percent_Identity=30.5555555555556, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1789378, Length=260, Percent_Identity=27.3076923076923, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI87082100, Length=255, Percent_Identity=28.2352941176471, Blast_Score=69, Evalue=4e-13,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR014358
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: =1.3.1.9 [H]

Molecular weight: Translated: 31256; Mature: 31125

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIAQSPKDQGQESNHSVTKGNFMGNELLAGKRGLIMGLANDKSIAWGIAKTCADAGAEL
CCCCCCCHHCCCCCCCCEEECCCCCCHHHCCCCCEEEEECCCCCHHHHHHHHHHHCCCEE
AFSYQGEALKKRVGPLAEKLGSDIVLPCDVSDEASMDALFDALKDKWGTLDFIVHAIGFS
EEECCCHHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCC
DKGELRGRYVDTTRDNFAMTMDISVYSFTSVVQRAEKMMNPGGSCLTMTYYGAEKVMPHY
CCCCCCCEEECCCCCCEEEEEEEHHHHHHHHHHHHHHHHCCCCCEEEEEEECHHHCCCCC
NVMGVAKAALEASVQYLAEDLGKDGIRVNAISAGPIKTLAASGIGDFRYIMKWNEYNSPL
CHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHCCCCCEEEEEEECCCCCCH
RRNVTIDDVGKAALFLLSDLGSGTTGENLHVDAGYHVVGMKAVDAPDMSKE
HCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCEEEEEEEEECCCCCCCC
>Mature Secondary Structure 
AIAQSPKDQGQESNHSVTKGNFMGNELLAGKRGLIMGLANDKSIAWGIAKTCADAGAEL
CCCCCCHHCCCCCCCCEEECCCCCCHHHCCCCCEEEEECCCCCHHHHHHHHHHHCCCEE
AFSYQGEALKKRVGPLAEKLGSDIVLPCDVSDEASMDALFDALKDKWGTLDFIVHAIGFS
EEECCCHHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCC
DKGELRGRYVDTTRDNFAMTMDISVYSFTSVVQRAEKMMNPGGSCLTMTYYGAEKVMPHY
CCCCCCCEEECCCCCCEEEEEEEHHHHHHHHHHHHHHHHCCCCCEEEEEEECHHHCCCCC
NVMGVAKAALEASVQYLAEDLGKDGIRVNAISAGPIKTLAASGIGDFRYIMKWNEYNSPL
CHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHCCCCCEEEEEEECCCCCCH
RRNVTIDDVGKAALFLLSDLGSGTTGENLHVDAGYHVVGMKAVDAPDMSKE
HCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCEEEEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]