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The map label for this gene is metH [H]

Identifier: 110680248

GI number: 110680248

Start: 2926061

End: 2927143

Strand: Direct

Name: metH [H]

Synonym: RD1_3057

Alternate gene names: 110680248

Gene position: 2926061-2927143 (Clockwise)

Preceding gene: 110680247

Following gene: 110680249

Centisome position: 70.8

GC content: 59.83

Gene sequence:

>1083_bases
ATGACAAAGACCATCGTCGAATCAAAAACCAAGACTGCGATCATCGGGTTCGATCAGCCCTTTTGCGTGATCGGGGAACG
CATCAACCCGACAGGGCGCAAGAAACTCGCGCTTGAACTGGAAGCAGGCGACTTTTCCACTGTCGAGGCCGATGCCATGG
CGCAGGTCGCGGCCGGGGCGACGGTGCTGGACATCAATTCGGGCGCTGTCTTTTCCAACAAGATGGCCGAAGATACCCGC
TATGCGGACAACAACTTTGTCGAACCGCCCTTGATGCGCGAGTTGGTCCTGCGCGTGCAGGCCATCGTTGATGTGCCGCT
TTGCATCGACAGTTCCGTACCCGGTGCGCTCGAATCAGGTCTCGAAGCGGCCGAGGGTCGCCCCTTGCTAAATTCGGTCA
CCGGCGAGGAAGAACGGCTGGAAGTGGTCTTGCCGCTGGTCAAGAAGTATAATGTGCCTGTCGTAGCTATTTCGAACGAC
GATACCGGCATTTCGGAGGATCCGGACGTACGATTTGCCGTCGCCAAAAAGATCGTCGAACGTGCCGCTGATTTCGGCAT
CCCGGCGCATGACATCGTGGTTGACCCCCTCGTGATGCCAATCGGAGCGATGGCCACCGCAGGGCATCAGGTATTCACGC
TGGTGCGTCGGCTGCGCGAGGAATTGGGTGTCAACACGACCTGCGGCGCATCCAACATCAGCTTTGGCTTGCCCAACCGT
CACGGCATCAACAACGCGTTTTTGCCAATGGCGATGGGTGCTGGCATGACCTCTGCCATTATGAATCCCGTCGCCCTGCC
CGTTTCCGCAGCCCGAATCGAAGCCAAGAAGATGGAACTGGCCGCGGCAGGTGTTGTGTTTCCCGAAGACATGGACCTTG
AGACCTTTTGCCAACTGACCGGTTTGGGTTCGACCCGACCGCGGCCCGGAAAAGAGATGGAGGCCATCCGTGCGGCGAAT
TTCCTGACCAACAATGACCCGCACGGGGGCGCCTGGATCGCGTTTAACAAGGAAGCCCCCAAGCCGGGCCAGGAAGGTCG
CGGACGTGCGGCGCGCACGGGTGGGCGGCGCCGTCGCGCGTGA

Upstream 100 bases:

>100_bases
CTTTCCACTCGGCGGGATCAAGACGAACGCCAACTGGGCCATCGAAAACGGCGGCTCCTCCGCAAAACCGGCAAACGCCT
GATCCAAGGACTTATTCCTG

Downstream 100 bases:

>100_bases
TTGGCTTTTTGAGCAGACAATTCTGGAACCCCCGTCGATGACATCGCCGGGGGTTTTTTTGTTTAAAAACAAAGATCTGT
CATGTCCGGCGAACCAATTT

Product: methyltetrahydrofolate:corrinoid/iron-sulfur protein methyltransferase

Products: NA

Alternate protein names: 5-methyltetrahydrofolate--homocysteine methyltransferase; Methionine synthase, vitamin-B12 dependent; MS [H]

Number of amino acids: Translated: 360; Mature: 359

Protein sequence:

>360_residues
MTKTIVESKTKTAIIGFDQPFCVIGERINPTGRKKLALELEAGDFSTVEADAMAQVAAGATVLDINSGAVFSNKMAEDTR
YADNNFVEPPLMRELVLRVQAIVDVPLCIDSSVPGALESGLEAAEGRPLLNSVTGEEERLEVVLPLVKKYNVPVVAISND
DTGISEDPDVRFAVAKKIVERAADFGIPAHDIVVDPLVMPIGAMATAGHQVFTLVRRLREELGVNTTCGASNISFGLPNR
HGINNAFLPMAMGAGMTSAIMNPVALPVSAARIEAKKMELAAAGVVFPEDMDLETFCQLTGLGSTRPRPGKEMEAIRAAN
FLTNNDPHGGAWIAFNKEAPKPGQEGRGRAARTGGRRRRA

Sequences:

>Translated_360_residues
MTKTIVESKTKTAIIGFDQPFCVIGERINPTGRKKLALELEAGDFSTVEADAMAQVAAGATVLDINSGAVFSNKMAEDTR
YADNNFVEPPLMRELVLRVQAIVDVPLCIDSSVPGALESGLEAAEGRPLLNSVTGEEERLEVVLPLVKKYNVPVVAISND
DTGISEDPDVRFAVAKKIVERAADFGIPAHDIVVDPLVMPIGAMATAGHQVFTLVRRLREELGVNTTCGASNISFGLPNR
HGINNAFLPMAMGAGMTSAIMNPVALPVSAARIEAKKMELAAAGVVFPEDMDLETFCQLTGLGSTRPRPGKEMEAIRAAN
FLTNNDPHGGAWIAFNKEAPKPGQEGRGRAARTGGRRRRA
>Mature_359_residues
TKTIVESKTKTAIIGFDQPFCVIGERINPTGRKKLALELEAGDFSTVEADAMAQVAAGATVLDINSGAVFSNKMAEDTRY
ADNNFVEPPLMRELVLRVQAIVDVPLCIDSSVPGALESGLEAAEGRPLLNSVTGEEERLEVVLPLVKKYNVPVVAISNDD
TGISEDPDVRFAVAKKIVERAADFGIPAHDIVVDPLVMPIGAMATAGHQVFTLVRRLREELGVNTTCGASNISFGLPNRH
GINNAFLPMAMGAGMTSAIMNPVALPVSAARIEAKKMELAAAGVVFPEDMDLETFCQLTGLGSTRPRPGKEMEAIRAANF
LTNNDPHGGAWIAFNKEAPKPGQEGRGRAARTGGRRRRA

Specific function: Catalyzes the transfer of a methyl group from methyl- cobalamin to homocysteine, yielding enzyme-bound cob(I)alamin and methionine. Subsequently, remethylates the cofactor using methyltetrahydrofolate [H]

COG id: COG1410

COG function: function code E; Methionine synthase I, cobalamin-binding domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pterin-binding domain [H]

Homologues:

Organism=Homo sapiens, GI169790923, Length=262, Percent_Identity=29.7709923664122, Blast_Score=99, Evalue=7e-21,
Organism=Escherichia coli, GI1790450, Length=267, Percent_Identity=26.2172284644195, Blast_Score=77, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17535405, Length=272, Percent_Identity=27.2058823529412, Blast_Score=98, Evalue=6e-21,

Paralogues:

None

Copy number: 513 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006158
- InterPro:   IPR011005
- InterPro:   IPR003759
- InterPro:   IPR011822
- InterPro:   IPR000489
- InterPro:   IPR003726
- InterPro:   IPR004223 [H]

Pfam domain/function: PF02310 B12-binding; PF02607 B12-binding_2; PF02965 Met_synt_B12; PF00809 Pterin_bind; PF02574 S-methyl_trans [H]

EC number: =2.1.1.13 [H]

Molecular weight: Translated: 38401; Mature: 38269

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: PS50972 PTERIN_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKTIVESKTKTAIIGFDQPFCVIGERINPTGRKKLALELEAGDFSTVEADAMAQVAAGA
CCCCHHCCCCCEEEEECCCCCEEECCCCCCCCCEEEEEEEECCCCCCCCHHHHHHHHCCC
TVLDINSGAVFSNKMAEDTRYADNNFVEPPLMRELVLRVQAIVDVPLCIDSSVPGALESG
EEEEECCCCEECCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCEEECCCCCHHHHHC
LEAAEGRPLLNSVTGEEERLEVVLPLVKKYNVPVVAISNDDTGISEDPDVRFAVAKKIVE
CHHHCCCCHHHHCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCHHHHHHHHHHH
RAADFGIPAHDIVVDPLVMPIGAMATAGHQVFTLVRRLREELGVNTTCGASNISFGLPNR
HHHHCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
HGINNAFLPMAMGAGMTSAIMNPVALPVSAARIEAKKMELAAAGVVFPEDMDLETFCQLT
CCCCCCHHHHHHCCCHHHHHHCCHHCCCHHHHHHHHHHHHHHCCEECCCCCCHHHHHHHH
GLGSTRPRPGKEMEAIRAANFLTNNDPHGGAWIAFNKEAPKPGQEGRGRAARTGGRRRRA
CCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCHHHCCCCCCCC
>Mature Secondary Structure 
TKTIVESKTKTAIIGFDQPFCVIGERINPTGRKKLALELEAGDFSTVEADAMAQVAAGA
CCCHHCCCCCEEEEECCCCCEEECCCCCCCCCEEEEEEEECCCCCCCCHHHHHHHHCCC
TVLDINSGAVFSNKMAEDTRYADNNFVEPPLMRELVLRVQAIVDVPLCIDSSVPGALESG
EEEEECCCCEECCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCEEECCCCCHHHHHC
LEAAEGRPLLNSVTGEEERLEVVLPLVKKYNVPVVAISNDDTGISEDPDVRFAVAKKIVE
CHHHCCCCHHHHCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCHHHHHHHHHHH
RAADFGIPAHDIVVDPLVMPIGAMATAGHQVFTLVRRLREELGVNTTCGASNISFGLPNR
HHHHCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
HGINNAFLPMAMGAGMTSAIMNPVALPVSAARIEAKKMELAAAGVVFPEDMDLETFCQLT
CCCCCCHHHHHHCCCHHHHHHCCHHCCCHHHHHHHHHHHHHHCCEECCCCCCHHHHHHHH
GLGSTRPRPGKEMEAIRAANFLTNNDPHGGAWIAFNKEAPKPGQEGRGRAARTGGRRRRA
CCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]