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The map label for this gene is tgt [H]

Identifier: 110680152

GI number: 110680152

Start: 2831666

End: 2832850

Strand: Reverse

Name: tgt [H]

Synonym: RD1_2952

Alternate gene names: 110680152

Gene position: 2832850-2831666 (Counterclockwise)

Preceding gene: 110680162

Following gene: 110680151

Centisome position: 68.54

GC content: 60.25

Gene sequence:

>1185_bases
ATGGGTGCAGCCATTAATCAAGGGCTTGGAACGAAGGGAAATTTGCGGCAAACGGACCGGATGAGATTCAGTTTTGACCT
ACAGGCCACGGATGGCCGCGCGCGCACAGGCGTGATTTCGACGCCGCGGGGCGACATTCGCACGCCGGCCTTCATGCCCG
TGGGCACCGCCGCGACCGTCAAGGCAATGCTGCCGGCGTCGGTGCGCCAGACCGGTGCTGATATCCTGCTGGGAAATACC
TACCATTTGATGCTGCGCCCGACGGCGGAGCGCATCGACCGGCTTGGCGGTTTGCACAGGTTCATGGACTGGGACGGCCC
GATCCTGACGGATTCGGGCGGGTTTCAGGTCATGAGCCTCGCCGCGTTGCGCAAGCTGACCGAAGAGGGCGTGAGTTTCA
AAAGTCATATCGACGGATCACGGCACATGCTGACGCCCGAGCGATCCATGGAAATCCAGCGCCTGCTCGGCTCTGACATC
GTCATGTGTTTTGACGAGTGTCCCGCACTGCCTGCTGATCGCGCGGCCATCGAAAAGAGCATGCAATTGTCGATGCGGTG
GGCAGCGCGGTCCAAGGACGCTTTTGGTGACCGGCCTGGCCACGCGCTTTTCGGTATTCAGCAGGGAGGGCTGGAAGAAG
ACCTGCGCGCAGAAAGTGCGGCAGCCCTGCGCGAGATTGAATTTGATGGCTACGCCGTGGGCGGGCTTGCGGTTGGCGAG
GGCCAGGCGGCGATGTTTGGCTGTCTGGACTATGCGCCGGAACAGTTGCCCCGCGACAAGCCGCGATACCTGATGGGGGT
GGGCAAACCGGATGACATTGTCGGGGCCGTGGCGCGTGGCATTGATATGATGGATTGTGTTCTGCCCAGCCGCTCCGGGC
GCACAGGGCAGGTCTTCACCCGTCGCGGTGTTGTGAACATCAAGAATGCGCGCCATGCGGATGATCCAAGGCCGCTGGAT
GAGCAGTGCAGCTGCCCCGCGTGCCGCAGCTATAGCCGCGCCTATCTGCATCATGTGTTCCGGTCGCAGGAAATCATCAG
TTCAATGCTTTTGACATGGCATAACCTGCATTATTATCAGGAGCTTATGGCGGGCATGCGGTCTGCGATCGCGCAAGGTA
CATTTGATCAATGGACCAGCGGTTTCATGGCTGAGCGTGCTTTGGGCGATATCGAACCAATTTAG

Upstream 100 bases:

>100_bases
ACCAGGTGGTGCGCGCGTTGGCCGTAAGCGCGTTGATGCGGTCGAGGTGATCCTGATCTGTCACGGTCTGACCTTTGGCT
TTTTATAAGGCGACTGTGGA

Downstream 100 bases:

>100_bases
CTTATACTGACATTATTTTTTATCCGCGAAGTTCTCCCCGCGATGCTGGATTTCAGCGGGGATTAACCTGTGCTTAAACC
AAATGCCTTTACACAGGCGC

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase [H]

Number of amino acids: Translated: 394; Mature: 393

Protein sequence:

>394_residues
MGAAINQGLGTKGNLRQTDRMRFSFDLQATDGRARTGVISTPRGDIRTPAFMPVGTAATVKAMLPASVRQTGADILLGNT
YHLMLRPTAERIDRLGGLHRFMDWDGPILTDSGGFQVMSLAALRKLTEEGVSFKSHIDGSRHMLTPERSMEIQRLLGSDI
VMCFDECPALPADRAAIEKSMQLSMRWAARSKDAFGDRPGHALFGIQQGGLEEDLRAESAAALREIEFDGYAVGGLAVGE
GQAAMFGCLDYAPEQLPRDKPRYLMGVGKPDDIVGAVARGIDMMDCVLPSRSGRTGQVFTRRGVVNIKNARHADDPRPLD
EQCSCPACRSYSRAYLHHVFRSQEIISSMLLTWHNLHYYQELMAGMRSAIAQGTFDQWTSGFMAERALGDIEPI

Sequences:

>Translated_394_residues
MGAAINQGLGTKGNLRQTDRMRFSFDLQATDGRARTGVISTPRGDIRTPAFMPVGTAATVKAMLPASVRQTGADILLGNT
YHLMLRPTAERIDRLGGLHRFMDWDGPILTDSGGFQVMSLAALRKLTEEGVSFKSHIDGSRHMLTPERSMEIQRLLGSDI
VMCFDECPALPADRAAIEKSMQLSMRWAARSKDAFGDRPGHALFGIQQGGLEEDLRAESAAALREIEFDGYAVGGLAVGE
GQAAMFGCLDYAPEQLPRDKPRYLMGVGKPDDIVGAVARGIDMMDCVLPSRSGRTGQVFTRRGVVNIKNARHADDPRPLD
EQCSCPACRSYSRAYLHHVFRSQEIISSMLLTWHNLHYYQELMAGMRSAIAQGTFDQWTSGFMAERALGDIEPI
>Mature_393_residues
GAAINQGLGTKGNLRQTDRMRFSFDLQATDGRARTGVISTPRGDIRTPAFMPVGTAATVKAMLPASVRQTGADILLGNTY
HLMLRPTAERIDRLGGLHRFMDWDGPILTDSGGFQVMSLAALRKLTEEGVSFKSHIDGSRHMLTPERSMEIQRLLGSDIV
MCFDECPALPADRAAIEKSMQLSMRWAARSKDAFGDRPGHALFGIQQGGLEEDLRAESAAALREIEFDGYAVGGLAVGEG
QAAMFGCLDYAPEQLPRDKPRYLMGVGKPDDIVGAVARGIDMMDCVLPSRSGRTGQVFTRRGVVNIKNARHADDPRPLDE
QCSCPACRSYSRAYLHHVFRSQEIISSMLLTWHNLHYYQELMAGMRSAIAQGTFDQWTSGFMAERALGDIEPI

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI13654276, Length=376, Percent_Identity=40.1595744680851, Blast_Score=293, Evalue=2e-79,
Organism=Escherichia coli, GI1786607, Length=362, Percent_Identity=55.8011049723757, Blast_Score=404, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI17544680, Length=362, Percent_Identity=38.9502762430939, Blast_Score=263, Evalue=1e-70,
Organism=Caenorhabditis elegans, GI25146502, Length=154, Percent_Identity=28.5714285714286, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI19920510, Length=357, Percent_Identity=43.6974789915966, Blast_Score=309, Evalue=2e-84,
Organism=Drosophila melanogaster, GI21355311, Length=259, Percent_Identity=23.9382239382239, Blast_Score=85, Evalue=7e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004803
- InterPro:   IPR002616 [H]

Pfam domain/function: PF01702 TGT [H]

EC number: =2.4.2.29 [H]

Molecular weight: Translated: 43270; Mature: 43139

Theoretical pI: Translated: 7.04; Mature: 7.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
6.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGAAINQGLGTKGNLRQTDRMRFSFDLQATDGRARTGVISTPRGDIRTPAFMPVGTAATV
CCCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCCCCEEECCCCCCCCCCCCCCCCHHHH
KAMLPASVRQTGADILLGNTYHLMLRPTAERIDRLGGLHRFMDWDGPILTDSGGFQVMSL
HHHHHHHHHHCCCCEEECCEEEEEECCHHHHHHHHHHHHHHHCCCCCEEECCCCHHHHHH
AALRKLTEEGVSFKSHIDGSRHMLTPERSMEIQRLLGSDIVMCFDECPALPADRAAIEKS
HHHHHHHHHCCCHHHHCCCCCCCCCCHHHHHHHHHHCCHHEEHHHHCCCCCCHHHHHHHH
MQLSMRWAARSKDAFGDRPGHALFGIQQGGLEEDLRAESAAALREIEFDGYAVGGLAVGE
HHHHHHHHHCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCEEECCEEECC
GQAAMFGCLDYAPEQLPRDKPRYLMGVGKPDDIVGAVARGIDMMDCVLPSRSGRTGQVFT
CCHHHHHHHHHCHHHCCCCCCCEEEECCCCHHHHHHHHHCCHHHHHHCCCCCCCCCCEEE
RRGVVNIKNARHADDPRPLDEQCSCPACRSYSRAYLHHVFRSQEIISSMLLTWHNLHYYQ
ECCCEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELMAGMRSAIAQGTFDQWTSGFMAERALGDIEPI
HHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
GAAINQGLGTKGNLRQTDRMRFSFDLQATDGRARTGVISTPRGDIRTPAFMPVGTAATV
CCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCCCCEEECCCCCCCCCCCCCCCCHHHH
KAMLPASVRQTGADILLGNTYHLMLRPTAERIDRLGGLHRFMDWDGPILTDSGGFQVMSL
HHHHHHHHHHCCCCEEECCEEEEEECCHHHHHHHHHHHHHHHCCCCCEEECCCCHHHHHH
AALRKLTEEGVSFKSHIDGSRHMLTPERSMEIQRLLGSDIVMCFDECPALPADRAAIEKS
HHHHHHHHHCCCHHHHCCCCCCCCCCHHHHHHHHHHCCHHEEHHHHCCCCCCHHHHHHHH
MQLSMRWAARSKDAFGDRPGHALFGIQQGGLEEDLRAESAAALREIEFDGYAVGGLAVGE
HHHHHHHHHCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCEEECCEEECC
GQAAMFGCLDYAPEQLPRDKPRYLMGVGKPDDIVGAVARGIDMMDCVLPSRSGRTGQVFT
CCHHHHHHHHHCHHHCCCCCCCEEEECCCCHHHHHHHHHCCHHHHHHCCCCCCCCCCEEE
RRGVVNIKNARHADDPRPLDEQCSCPACRSYSRAYLHHVFRSQEIISSMLLTWHNLHYYQ
ECCCEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELMAGMRSAIAQGTFDQWTSGFMAERALGDIEPI
HHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA