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The map label for this gene is fdhB [H]

Identifier: 110680099

GI number: 110680099

Start: 2775421

End: 2776017

Strand: Reverse

Name: fdhB [H]

Synonym: RD1_2896

Alternate gene names: 110680099

Gene position: 2776017-2775421 (Counterclockwise)

Preceding gene: 110680100

Following gene: 110680098

Centisome position: 67.17

GC content: 62.14

Gene sequence:

>597_bases
ATGGCCGCTAGAGCAAAATTTCTCGTCGACGCTGAACGCTGCATTGAGTGCAACGCCTGTGTCACCGCCTGTAAGAACGA
GCACGAAGTGCCGTGGGGCATCAACCGCCGGAAGGTCGTCACCCTGAATGACGGCAAGCCGGGTGAACGGTCGATCTCCG
TCGCCTGCATGCACTGTTCAGACGCGCCTTGCATGGCCGTCTGTCCGGTGGATTGTTTCTACCAGAACGAAGAGGGCGTG
GTCCTGCACTCCAAGGACCTGTGCATCGGCTGCGGGTACTGCTTTTATGCATGCCCCTTCGGCGCACCCCAGTTCCCGCA
GGCGGGCAACTTCGGATCCCGTGGCAAGATGGACAAATGTACCTTCTGCGCAGGCGGTCCCGAAGAAAACCACTCCACCG
CCGAATTCCAGAAGTATGGCCGCAACCGGATCGCGGAAGGCAAGCTGCCGATCTGCGCCGAGATGTGCGCCACCAAGGCG
CTTCTGGCCGGTGATGGCGACGTTGTCGCGGGCATCTACCGCGAGCGCGTCGTGGCGCGTGGCTTTGGCTCCGGCGCATG
GGGCTGGGGCACGGCATACGACCAAAAAGGCGGCTGA

Upstream 100 bases:

>100_bases
CAACACCGCACAGACCTATGGCTATGACAGCGTAACCCAGATGCAAGAGACGAAAGCCACTCTTTGCAAAATCTGGGCAG
CATAAGGGAGGACATGAACA

Downstream 100 bases:

>100_bases
CAGGCCCCTTTGTCGCAATACCTAACTGGGGGCGGTTCTGCTGAGGGCCGCCCCTCGCTATCTTGGATAGGATAAAAACA
TGCTGCGTCAGATCTACGCT

Product: formate dehydrogenase Fe-S subunit

Products: CO2; NADH

Alternate protein names: NA

Number of amino acids: Translated: 198; Mature: 197

Protein sequence:

>198_residues
MAARAKFLVDAERCIECNACVTACKNEHEVPWGINRRKVVTLNDGKPGERSISVACMHCSDAPCMAVCPVDCFYQNEEGV
VLHSKDLCIGCGYCFYACPFGAPQFPQAGNFGSRGKMDKCTFCAGGPEENHSTAEFQKYGRNRIAEGKLPICAEMCATKA
LLAGDGDVVAGIYRERVVARGFGSGAWGWGTAYDQKGG

Sequences:

>Translated_198_residues
MAARAKFLVDAERCIECNACVTACKNEHEVPWGINRRKVVTLNDGKPGERSISVACMHCSDAPCMAVCPVDCFYQNEEGV
VLHSKDLCIGCGYCFYACPFGAPQFPQAGNFGSRGKMDKCTFCAGGPEENHSTAEFQKYGRNRIAEGKLPICAEMCATKA
LLAGDGDVVAGIYRERVVARGFGSGAWGWGTAYDQKGG
>Mature_197_residues
AARAKFLVDAERCIECNACVTACKNEHEVPWGINRRKVVTLNDGKPGERSISVACMHCSDAPCMAVCPVDCFYQNEEGVV
LHSKDLCIGCGYCFYACPFGAPQFPQAGNFGSRGKMDKCTFCAGGPEENHSTAEFQKYGRNRIAEGKLPICAEMCATKAL
LAGDGDVVAGIYRERVVARGFGSGAWGWGTAYDQKGG

Specific function: This chain is an electron transfer unit containing 18 cysteine residues, 16 of which occur in four clusters [H]

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1787122, Length=178, Percent_Identity=32.5842696629214, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1787872, Length=175, Percent_Identity=32.5714285714286, Blast_Score=97, Evalue=9e-22,
Organism=Escherichia coli, GI226510944, Length=170, Percent_Identity=34.1176470588235, Blast_Score=91, Evalue=7e-20,
Organism=Escherichia coli, GI1789370, Length=198, Percent_Identity=28.2828282828283, Blast_Score=83, Evalue=1e-17,
Organism=Escherichia coli, GI2367345, Length=173, Percent_Identity=32.9479768786127, Blast_Score=82, Evalue=2e-17,
Organism=Escherichia coli, GI1790326, Length=202, Percent_Identity=27.7227722772277, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1787749, Length=204, Percent_Identity=26.9607843137255, Blast_Score=68, Evalue=3e-13,
Organism=Escherichia coli, GI87082114, Length=89, Percent_Identity=38.2022471910112, Blast_Score=67, Evalue=7e-13,
Organism=Escherichia coli, GI2367245, Length=180, Percent_Identity=30.5555555555556, Blast_Score=63, Evalue=1e-11,
Organism=Escherichia coli, GI1789079, Length=200, Percent_Identity=28, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI87082179, Length=184, Percent_Identity=29.3478260869565, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR000813 [H]

Pfam domain/function: PF00037 Fer4 [H]

EC number: 1.2.1.2

Molecular weight: Translated: 21300; Mature: 21169

Theoretical pI: Translated: 7.14; Mature: 7.14

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

8.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
11.1 %Cys+Met (Translated Protein)
8.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
10.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAARAKFLVDAERCIECNACVTACKNEHEVPWGINRRKVVTLNDGKPGERSISVACMHCS
CCCCCCEEECHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCCEEEEEEEECC
DAPCMAVCPVDCFYQNEEGVVLHSKDLCIGCGYCFYACPFGAPQFPQAGNFGSRGKMDKC
CCCEEEECCCCCEEECCCCEEEECCCCEEECCHHHEECCCCCCCCCCCCCCCCCCCCCCE
TFCAGGPEENHSTAEFQKYGRNRIAEGKLPICAEMCATKALLAGDGDVVAGIYRERVVAR
EEECCCCCCCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHEECCCCCEEHHHHHHHHHHC
GFGSGAWGWGTAYDQKGG
CCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AARAKFLVDAERCIECNACVTACKNEHEVPWGINRRKVVTLNDGKPGERSISVACMHCS
CCCCCEEECHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCCEEEEEEEECC
DAPCMAVCPVDCFYQNEEGVVLHSKDLCIGCGYCFYACPFGAPQFPQAGNFGSRGKMDKC
CCCEEEECCCCCEEECCCCEEEECCCCEEECCHHHEECCCCCCCCCCCCCCCCCCCCCCE
TFCAGGPEENHSTAEFQKYGRNRIAEGKLPICAEMCATKALLAGDGDVVAGIYRERVVAR
EEECCCCCCCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHEECCCCCEEHHHHHHHHHHC
GFGSGAWGWGTAYDQKGG
CCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; NAD+

Specific reaction: formate + NAD+ = CO2 + NADH

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1781728; 14500908 [H]