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The map label for this gene is fdhI [H]

Identifier: 110680098

GI number: 110680098

Start: 2774151

End: 2775341

Strand: Reverse

Name: fdhI [H]

Synonym: RD1_2895

Alternate gene names: 110680098

Gene position: 2775341-2774151 (Counterclockwise)

Preceding gene: 110680099

Following gene: 110680095

Centisome position: 67.15

GC content: 58.52

Gene sequence:

>1191_bases
ATGCTGCGTCAGATCTACGCTTTGTTGATTACTTTTTCCCTGTGCTTTCCTGCCTTTGCACAGGAAGCACCTGTTGGCGA
AACCGACCGCTCCGCGACCGGGGGGGCGACAACGCTCGAAGACATCATGAAGCGCCAGGAAGGTCTCAAGGTCGGGAATG
AATTCCGAAGCGGCCTGACCGGGAACCCCGCCGCGGCAAAACCAACGACAGAGCAACTGGGCACGCTTGGCGGCGCGTCT
GACCCGGACCTGTGGCGCGCGCTGCGCTTTGACAGCGCGGATATTACCACGCAGATACAACACCCCGCAGCAACGGTTCT
CATCCAGGATACGGGCATGTGGTGGTTGCAGTTCCGCGAAGGGCCGCTGACGACCTATGGCTTCGGCCTGATCCTGGTTA
CGATGGGCGCGCTGGTGCTGTTCTACCTGATCCGCGGGCGCATCCGCATTGACGGGGAAAGAACCGGCAGGACGATCCTG
CGCTTCAAATCCATTGAACGCTTCGCCCATTGGCTCTTGGCCGGGTCGTTTATTCTGCTGGGTCTTACAGGGGTCATATC
GCTATTGGGTCGCCAATTCCTGATCCCTGCCTTGGGGCCGGAGGCGTTCTCGACCATCGCGATTGGCGCCAAGTGGATAC
ATAACAACGTGTCATGGGCGTTCATGCTGGCCATCGTCATGATTTTTTTCCTCTGGGTCGTGCACAACCTGCCGCACCGC
TCTGACATCTACTGGCTGCGCAAGGGGGGCGGTATCTTCGGCGGGGGGCACCCGCCGGCCAAGAAGTTCAACGCCGGTCA
GAAGTTCATCTTCTGGTCCGTGATCGTTCTGGGGGGCTCTATCTCGGCCTCTGGTTTGTCTTTGTTGTTCCCGTTCGAAC
TGCCCATGTTTGCCGCGACATTTGCCAAGCTGAACGATTTTGGCATCCCCCAGTTGATCGGGTTGGGCGAATTGAACACA
GACCTCGCGCCGCATACGGAAATGCAATATGCGCAGCTCTGGCATTCGATCGTCAGCTTCATCCTCTTTGCGATTGTGAT
TGCGCATATCTATATCGGGTCGGTCGGCATGGAGGGTGCCTATGACGCCATGGGATCGGGCGAAGTCGAAGAGCAATGGG
CGCGCGAGCACCACAGCCTTTGGGTCGAAGAGGTCAAAGCCAAGGAAGCGCAAGCGGGGAGGCCATCATGA

Upstream 100 bases:

>100_bases
TACGACCAAAAAGGCGGCTGACAGGCCCCTTTGTCGCAATACCTAACTGGGGGCGGTTCTGCTGAGGGCCGCCCCTCGCT
ATCTTGGATAGGATAAAAAC

Downstream 100 bases:

>100_bases
GAGAGATTTTCCTCGCCTTTGCAACGATGGGTGTGATCGCGGTCACGGCCAGCTACGTCTTGCCGGAATTGGGGTTTTCT
TCGCAGGCCCAGACAACGGG

Product: formate dehydrogenase, gamma subunit, putative

Products: CO2; NADH

Alternate protein names: Aerobic formate dehydrogenase cytochrome b556 subunit; FDH-Z subunit gamma; Formate dehydrogenase-O subunit gamma [H]

Number of amino acids: Translated: 396; Mature: 396

Protein sequence:

>396_residues
MLRQIYALLITFSLCFPAFAQEAPVGETDRSATGGATTLEDIMKRQEGLKVGNEFRSGLTGNPAAAKPTTEQLGTLGGAS
DPDLWRALRFDSADITTQIQHPAATVLIQDTGMWWLQFREGPLTTYGFGLILVTMGALVLFYLIRGRIRIDGERTGRTIL
RFKSIERFAHWLLAGSFILLGLTGVISLLGRQFLIPALGPEAFSTIAIGAKWIHNNVSWAFMLAIVMIFFLWVVHNLPHR
SDIYWLRKGGGIFGGGHPPAKKFNAGQKFIFWSVIVLGGSISASGLSLLFPFELPMFAATFAKLNDFGIPQLIGLGELNT
DLAPHTEMQYAQLWHSIVSFILFAIVIAHIYIGSVGMEGAYDAMGSGEVEEQWAREHHSLWVEEVKAKEAQAGRPS

Sequences:

>Translated_396_residues
MLRQIYALLITFSLCFPAFAQEAPVGETDRSATGGATTLEDIMKRQEGLKVGNEFRSGLTGNPAAAKPTTEQLGTLGGAS
DPDLWRALRFDSADITTQIQHPAATVLIQDTGMWWLQFREGPLTTYGFGLILVTMGALVLFYLIRGRIRIDGERTGRTIL
RFKSIERFAHWLLAGSFILLGLTGVISLLGRQFLIPALGPEAFSTIAIGAKWIHNNVSWAFMLAIVMIFFLWVVHNLPHR
SDIYWLRKGGGIFGGGHPPAKKFNAGQKFIFWSVIVLGGSISASGLSLLFPFELPMFAATFAKLNDFGIPQLIGLGELNT
DLAPHTEMQYAQLWHSIVSFILFAIVIAHIYIGSVGMEGAYDAMGSGEVEEQWAREHHSLWVEEVKAKEAQAGRPS
>Mature_396_residues
MLRQIYALLITFSLCFPAFAQEAPVGETDRSATGGATTLEDIMKRQEGLKVGNEFRSGLTGNPAAAKPTTEQLGTLGGAS
DPDLWRALRFDSADITTQIQHPAATVLIQDTGMWWLQFREGPLTTYGFGLILVTMGALVLFYLIRGRIRIDGERTGRTIL
RFKSIERFAHWLLAGSFILLGLTGVISLLGRQFLIPALGPEAFSTIAIGAKWIHNNVSWAFMLAIVMIFFLWVVHNLPHR
SDIYWLRKGGGIFGGGHPPAKKFNAGQKFIFWSVIVLGGSISASGLSLLFPFELPMFAATFAKLNDFGIPQLIGLGELNT
DLAPHTEMQYAQLWHSIVSFILFAIVIAHIYIGSVGMEGAYDAMGSGEVEEQWAREHHSLWVEEVKAKEAQAGRPS

Specific function: Allows to use formate as major electron donor during aerobic respiration. Subunit gamma is probably the cytochrome b556(FDO) component of the formate dehydrogenase [H]

COG id: COG2864

COG function: function code C; Cytochrome b subunit of formate dehydrogenase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the formate dehydrogenase gamma subunit family [H]

Homologues:

Organism=Escherichia coli, GI1790325, Length=238, Percent_Identity=26.890756302521, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1787750, Length=241, Percent_Identity=27.3858921161826, Blast_Score=78, Evalue=9e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005797
- InterPro:   IPR016174
- InterPro:   IPR006471 [H]

Pfam domain/function: PF00033 Cytochrom_B_N [H]

EC number: 1.2.1.2

Molecular weight: Translated: 43567; Mature: 43567

Theoretical pI: Translated: 7.14; Mature: 7.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRQIYALLITFSLCFPAFAQEAPVGETDRSATGGATTLEDIMKRQEGLKVGNEFRSGLT
CHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCHHHHHHHCCCC
GNPAAAKPTTEQLGTLGGASDPDLWRALRFDSADITTQIQHPAATVLIQDTGMWWLQFRE
CCCCCCCCCHHHHHCCCCCCCHHHHHHHHCCCCCCEEEECCCEEEEEEEECCEEEEEEEC
GPLTTYGFGLILVTMGALVLFYLIRGRIRIDGERTGRTILRFKSIERFAHWLLAGSFILL
CCCCHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
GLTGVISLLGRQFLIPALGPEAFSTIAIGAKWIHNNVSWAFMLAIVMIFFLWVVHNLPHR
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCC
SDIYWLRKGGGIFGGGHPPAKKFNAGQKFIFWSVIVLGGSISASGLSLLFPFELPMFAAT
CCEEEEECCCCCCCCCCCCHHHCCCCCHHHHHHHHHHCCCCCCCCCEEEECCHHHHHHHH
FAKLNDFGIPQLIGLGELNTDLAPHTEMQYAQLWHSIVSFILFAIVIAHIYIGSVGMEGA
HHHHHCCCCCHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
YDAMGSGEVEEQWAREHHSLWVEEVKAKEAQAGRPS
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MLRQIYALLITFSLCFPAFAQEAPVGETDRSATGGATTLEDIMKRQEGLKVGNEFRSGLT
CHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCHHHHHHHCCCC
GNPAAAKPTTEQLGTLGGASDPDLWRALRFDSADITTQIQHPAATVLIQDTGMWWLQFRE
CCCCCCCCCHHHHHCCCCCCCHHHHHHHHCCCCCCEEEECCCEEEEEEEECCEEEEEEEC
GPLTTYGFGLILVTMGALVLFYLIRGRIRIDGERTGRTILRFKSIERFAHWLLAGSFILL
CCCCHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
GLTGVISLLGRQFLIPALGPEAFSTIAIGAKWIHNNVSWAFMLAIVMIFFLWVVHNLPHR
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCC
SDIYWLRKGGGIFGGGHPPAKKFNAGQKFIFWSVIVLGGSISASGLSLLFPFELPMFAAT
CCEEEEECCCCCCCCCCCCHHHCCCCCHHHHHHHHHHCCCCCCCCCEEEECCHHHHHHHH
FAKLNDFGIPQLIGLGELNTDLAPHTEMQYAQLWHSIVSFILFAIVIAHIYIGSVGMEGA
HHHHHCCCCCHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
YDAMGSGEVEEQWAREHHSLWVEEVKAKEAQAGRPS
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; NAD+

Specific reaction: formate + NAD+ = CO2 + NADH

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]