Click here to switch to the map view.

The map label for this gene is iclR [H]

Identifier: 110680096

GI number: 110680096

Start: 2772262

End: 2773119

Strand: Direct

Name: iclR [H]

Synonym: RD1_2893

Alternate gene names: 110680096

Gene position: 2772262-2773119 (Clockwise)

Preceding gene: 110680089

Following gene: 110680097

Centisome position: 67.07

GC content: 60.96

Gene sequence:

>858_bases
ATGAACACACAGAAAAGGCCGCGCGGTCGCCCGAAATCGCAGTTCAAGGAAAGCACCGCCGGTACGATGCAATCGCTGGA
TCGTGCCTTGGGTGTTCTGGTGGCGGTGGCCAAGTCGGAACAGGCGACGCTGACCGACCTGTCGCTTTCGCTCGGTGTGC
CGACGGCCACCACGCACCGCATTCTGACGACGCTGCAAAAACACCGTTTTGTTGCCTTCAACGAAGAACACCAAAGCTGG
AGCATCGGGATCGAAGCCTACCGTACCGGTGCCTCCTTCATGAACCGGACCAGCCTGACCGAAGTGAGCCGCCCGATCAT
GCGCTTGCTGATGGAAAAGACGGGGGAGACCGCCAACCTTGCCATCCCGGACGGAGCTGAAGTCGTCTTTGTTGAACAGA
TCGAAACGCTCCACCCGATCCGCGCGTTTTTCGCACGGGGCGCACGCACGTCGATGCACGCTTCTGGCATTGGCAAGGCC
ATTCTGTCCACGATGAGCGAAGAACACGTCAGGCGACTGCTTATCTCGTCAGGCTTGCAGGCTTTTACGGACAATACGCT
CACATCCCCGGCATCGCTCTTTGCCGACCTCACCGAGACGCGGGCGCGCGGCTATTCCTATGATCGCGAAGAACGTTACC
TCGGCATGTCCTGCATCGGCAGTGCGATTTTCGACGAGCGCGGTGAGGCCTGTGGGGGCGTATCCATCTCAGGGCCAAGC
ACGCGGTTTGACAGCCTGCGTGTTCCTGAACTGGGGGCCATCGTGGCCGAGGCTGCAGGGCAGATTTCGCAACTCATCGG
CGGGCGCGGCCGGGAGCCTGCATCAAGCCTGCCTGCCTCAAACACCCCCACGGCGTGA

Upstream 100 bases:

>100_bases
ATTTATTTGGATATTTTCTTTTCATTATTTTCCATTTTCGCAATTTGGAAATTATTTTTATTTCTGATACGATATTAGGG
CAAACAGCCAGGGTATCCCC

Downstream 100 bases:

>100_bases
CCCACAACCGCGCGGTGTAATCACCGATTTGCGACGTGCAACCTCTTGTTTGCACACAACGAATACCTAAATTATTCTCA
TATAAACCTGTCATCAAAAA

Product: acetate operon repressor, putative

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 285; Mature: 285

Protein sequence:

>285_residues
MNTQKRPRGRPKSQFKESTAGTMQSLDRALGVLVAVAKSEQATLTDLSLSLGVPTATTHRILTTLQKHRFVAFNEEHQSW
SIGIEAYRTGASFMNRTSLTEVSRPIMRLLMEKTGETANLAIPDGAEVVFVEQIETLHPIRAFFARGARTSMHASGIGKA
ILSTMSEEHVRRLLISSGLQAFTDNTLTSPASLFADLTETRARGYSYDREERYLGMSCIGSAIFDERGEACGGVSISGPS
TRFDSLRVPELGAIVAEAAGQISQLIGGRGREPASSLPASNTPTA

Sequences:

>Translated_285_residues
MNTQKRPRGRPKSQFKESTAGTMQSLDRALGVLVAVAKSEQATLTDLSLSLGVPTATTHRILTTLQKHRFVAFNEEHQSW
SIGIEAYRTGASFMNRTSLTEVSRPIMRLLMEKTGETANLAIPDGAEVVFVEQIETLHPIRAFFARGARTSMHASGIGKA
ILSTMSEEHVRRLLISSGLQAFTDNTLTSPASLFADLTETRARGYSYDREERYLGMSCIGSAIFDERGEACGGVSISGPS
TRFDSLRVPELGAIVAEAAGQISQLIGGRGREPASSLPASNTPTA
>Mature_285_residues
MNTQKRPRGRPKSQFKESTAGTMQSLDRALGVLVAVAKSEQATLTDLSLSLGVPTATTHRILTTLQKHRFVAFNEEHQSW
SIGIEAYRTGASFMNRTSLTEVSRPIMRLLMEKTGETANLAIPDGAEVVFVEQIETLHPIRAFFARGARTSMHASGIGKA
ILSTMSEEHVRRLLISSGLQAFTDNTLTSPASLFADLTETRARGYSYDREERYLGMSCIGSAIFDERGEACGGVSISGPS
TRFDSLRVPELGAIVAEAAGQISQLIGGRGREPASSLPASNTPTA

Specific function: Regulation of the glyoxylate bypass operon (aceBAK), which encodes isocitrate lyase, malate synthase as well as isocitrate dehydrogenase kinase/phosphorylase. Glyoxylate disrupts the interaction with the promoter by favoring the inactive dimeric form. Pyr

COG id: COG1414

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 iclR-ED (iclR effector binding) domain [H]

Homologues:

Organism=Escherichia coli, GI87082359, Length=264, Percent_Identity=42.4242424242424, Blast_Score=209, Evalue=2e-55,
Organism=Escherichia coli, GI1786715, Length=266, Percent_Identity=37.218045112782, Blast_Score=183, Evalue=1e-47,
Organism=Escherichia coli, GI1788131, Length=246, Percent_Identity=28.4552845528455, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1786468, Length=248, Percent_Identity=29.0322580645161, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI1788581, Length=248, Percent_Identity=24.5967741935484, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1790752, Length=250, Percent_Identity=24, Blast_Score=88, Evalue=6e-19,
Organism=Escherichia coli, GI1789999, Length=259, Percent_Identity=27.4131274131274, Blast_Score=82, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014757
- InterPro:   IPR005471
- InterPro:   IPR011991 [H]

Pfam domain/function: PF09339 HTH_IclR; PF01614 IclR [H]

EC number: NA

Molecular weight: Translated: 30748; Mature: 30748

Theoretical pI: Translated: 8.72; Mature: 8.72

Prosite motif: PS51077 HTH_ICLR ; PS51078 ICLR_ED ; PS00354 HMGI_Y ; PS00070 ALDEHYDE_DEHYDR_CYS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTQKRPRGRPKSQFKESTAGTMQSLDRALGVLVAVAKSEQATLTDLSLSLGVPTATTHR
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCHHHHH
ILTTLQKHRFVAFNEEHQSWSIGIEAYRTGASFMNRTSLTEVSRPIMRLLMEKTGETANL
HHHHHHHHCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEE
AIPDGAEVVFVEQIETLHPIRAFFARGARTSMHASGIGKAILSTMSEEHVRRLLISSGLQ
ECCCCCCEEEHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHH
AFTDNTLTSPASLFADLTETRARGYSYDREERYLGMSCIGSAIFDERGEACGGVSISGPS
HHHCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCC
TRFDSLRVPELGAIVAEAAGQISQLIGGRGREPASSLPASNTPTA
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHCCCCCCCCCC
>Mature Secondary Structure
MNTQKRPRGRPKSQFKESTAGTMQSLDRALGVLVAVAKSEQATLTDLSLSLGVPTATTHR
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCHHHHH
ILTTLQKHRFVAFNEEHQSWSIGIEAYRTGASFMNRTSLTEVSRPIMRLLMEKTGETANL
HHHHHHHHCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEE
AIPDGAEVVFVEQIETLHPIRAFFARGARTSMHASGIGKAILSTMSEEHVRRLLISSGLQ
ECCCCCCEEEHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHH
AFTDNTLTSPASLFADLTETRARGYSYDREERYLGMSCIGSAIFDERGEACGGVSISGPS
HHHCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCC
TRFDSLRVPELGAIVAEAAGQISQLIGGRGREPASSLPASNTPTA
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2185227; 1995431; 8265357; 9278503; 1995429 [H]