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The map label for this gene is glgX [H]

Identifier: 110680077

GI number: 110680077

Start: 2744492

End: 2746588

Strand: Reverse

Name: glgX [H]

Synonym: RD1_2872

Alternate gene names: 110680077

Gene position: 2746588-2744492 (Counterclockwise)

Preceding gene: 110680078

Following gene: 110680076

Centisome position: 66.45

GC content: 61.23

Gene sequence:

>2097_bases
ATGGATTATCACTTCACATCCGGCAACCCGACACAGTTGGGCGCGCATCATGATGGGCAGGGCGTCAATTTCGCGGTCTT
TTCTGCGCATGCCAGTGCGATCGAACTGTGCCTTTTTACCCCAGACGGGCACACAGAAACGGCGCGCCTTGCCTTGCCGG
AACGCAGCGGTGATATCTGGCACGGCTATCTGCCGGGGCTGGCGGTGGGCACGGTTTATGGCTACCGCGCCCATGGGCCG
TATGCGCCGGAGCAGGGACACCGGTTTAACCCCAACAAACTTTTGCTCGACCCCTATACCCGGCAGCTTTGCGGTGCGTT
TAAAGATGACCCCGCGCTGCTTGGCTATGTAGAGGGCGACGCGGAAGAGGATCTGTCCTTTGATCCGCGCGACTCTGCCC
CCTACGTGGCCAAATCCGTGGTCCGCGACCCCGCGATTTTCCCCAAGGCGGGCACAGGTCCTCAAATCCCATGGGAAGAA
ACGTTGATCTATGAGGCGCATGTCAAAGGTCTGACCGCGCAAAATGACCGTGTTCCGGCCGATCTGCGCGGCACTTATGA
CGCCCTCGCCAGCGACGCGATGCTGGCGCATTTCAAACGGCTCGGCATCACGGCGGTTGAACTGCTGCCCGTCCATGCCT
TTGTGAATGACAGTTTTTTACTTAAGAAAGGTCTCACAAACTATTGGGGTTACAATACAATCGGGTTTTTTGCGCCCGAG
CCGCGCTATGTCGGCCCTGCAGGTATCGCTGGTTTTCAAAAGATGGTACAGCGCCTGCATGCAGCCGGGATCGAGGTCAT
TCTGGATGTGGTCTACAACCACACGGCCGAGGGCGATCAGCGCGGCCCGACGCTCAGTTTTCGCGGCCTCGACAATGCCT
CATACTATCGGCTGACCGATGCGGACCCGCGGTTTTACGTGAATGATACAGGCACGGGCAACACGCTGAACGTAGCGCAC
CCGCAGGTGCTGCGCATGGTGCTCGACAGCCTGCGGTTCTGGGTGGAGCATATGGGTGTCGACGGCTTTCGTTTCGATCT
GGCCACGACGCTGGCGCGCGAGCCGGATGGGTTTGACCCGCAGGGAGGGTTCATGGATGCACTGCGTCAGGACCCGGTGC
TGGGCGGGGTCAAACTGATTGCGGAACCTTGGGACATTGGACCCGGCGGCTACCAGTTGGGGGCTTACCCGCCCGAATTC
GCCGAGTGGAACGACGCCTATCGCGATACGGTGCGCCGCTTCTGGAAGGGCGACAGTCACAGCGCGCAGGACCTTGCCGC
GCGGCTGTTGGGATCGGCTGAGATCTTTGATCATCGGGGTCGGCGGGCGTGGTCCTCGCTCAACTTTCTGGCCGCGCATG
ACGGGTTCACGCTGGCGGATACGACAAGTTATGCAAGGCGGCACAATCAGGCGAACGGGGAGGGCAACCGCGACGGGCAT
CACGGCAATCACTCTGATTCTTGCGGTGTCGAAGGCCCCACAACGGATCCGGATATTCTGGCCCGCCGCGCCCGTCGTCA
GCGCAACATGCTGGCGACACTGTTCCTGTCGCAGGGCACGCCGATGCTGCTGGCCGGGGATGAGATGGGCAATTCGCAGG
CTGGCAACAACAATGCCTATTGTCAGGACAATGCCATCGGCTGGGTCGATTGGCAGGCGGATCAGGCGGGTCTTTTCGAT
TTTGTGCAACAGCTTTCAGCCTTCAGGGCTGCGCATCCGGTCCTGCGTCAATCACGTTTCCTGCATGGGGCTGCGCGCGT
GGCCGATGGCCGCCCGGATGTGGAATGGTCAGATGCCACCGGGGCTGCGCCGGATTGGCATGACCCGGATTTGTCGACCT
TTTGTCTGATGCTGCGCGGATCAGCCGAAGCATCGGTCGATACCGTTGGCGATGATGCGGTGCTGGTGGTCATCAACCGC
GCGCCTGATCCTGTGAAGATCGCACTGCCGCCGCCGCCCACCGGGCAGTGCTGGCAGATTGGCATGCGCACCGGGGCTGC
AGCGCGCGGCAAATTGGAAGCGGGGGAACTGGTGGTTGAAGCCGAAGAGGTCATGGCGCTGATCCTGTCGCCCATGGATG
CGCCCGATGCGTGGTGA

Upstream 100 bases:

>100_bases
GATGATGCGCTGCGCCATGCGTCACCCCGTGGGTTGGGAGACGTCCTCCAAAGCCTATCTTGAGGTTTACGATCGTCTTC
TGGGCGGATAACCGGGGCGG

Downstream 100 bases:

>100_bases
TGCCGCCTTGTCCCGCCTTGCGCAAGAGGTCGGCGTTGTGGCCGAATATCAGGACATGCAGGGCATCACGCATGTAACGC
CCCCCGAGACGCAGTGGGCG

Product: glycogen debranching enzyme

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 698; Mature: 698

Protein sequence:

>698_residues
MDYHFTSGNPTQLGAHHDGQGVNFAVFSAHASAIELCLFTPDGHTETARLALPERSGDIWHGYLPGLAVGTVYGYRAHGP
YAPEQGHRFNPNKLLLDPYTRQLCGAFKDDPALLGYVEGDAEEDLSFDPRDSAPYVAKSVVRDPAIFPKAGTGPQIPWEE
TLIYEAHVKGLTAQNDRVPADLRGTYDALASDAMLAHFKRLGITAVELLPVHAFVNDSFLLKKGLTNYWGYNTIGFFAPE
PRYVGPAGIAGFQKMVQRLHAAGIEVILDVVYNHTAEGDQRGPTLSFRGLDNASYYRLTDADPRFYVNDTGTGNTLNVAH
PQVLRMVLDSLRFWVEHMGVDGFRFDLATTLAREPDGFDPQGGFMDALRQDPVLGGVKLIAEPWDIGPGGYQLGAYPPEF
AEWNDAYRDTVRRFWKGDSHSAQDLAARLLGSAEIFDHRGRRAWSSLNFLAAHDGFTLADTTSYARRHNQANGEGNRDGH
HGNHSDSCGVEGPTTDPDILARRARRQRNMLATLFLSQGTPMLLAGDEMGNSQAGNNNAYCQDNAIGWVDWQADQAGLFD
FVQQLSAFRAAHPVLRQSRFLHGAARVADGRPDVEWSDATGAAPDWHDPDLSTFCLMLRGSAEASVDTVGDDAVLVVINR
APDPVKIALPPPPTGQCWQIGMRTGAAARGKLEAGELVVEAEEVMALILSPMDAPDAW

Sequences:

>Translated_698_residues
MDYHFTSGNPTQLGAHHDGQGVNFAVFSAHASAIELCLFTPDGHTETARLALPERSGDIWHGYLPGLAVGTVYGYRAHGP
YAPEQGHRFNPNKLLLDPYTRQLCGAFKDDPALLGYVEGDAEEDLSFDPRDSAPYVAKSVVRDPAIFPKAGTGPQIPWEE
TLIYEAHVKGLTAQNDRVPADLRGTYDALASDAMLAHFKRLGITAVELLPVHAFVNDSFLLKKGLTNYWGYNTIGFFAPE
PRYVGPAGIAGFQKMVQRLHAAGIEVILDVVYNHTAEGDQRGPTLSFRGLDNASYYRLTDADPRFYVNDTGTGNTLNVAH
PQVLRMVLDSLRFWVEHMGVDGFRFDLATTLAREPDGFDPQGGFMDALRQDPVLGGVKLIAEPWDIGPGGYQLGAYPPEF
AEWNDAYRDTVRRFWKGDSHSAQDLAARLLGSAEIFDHRGRRAWSSLNFLAAHDGFTLADTTSYARRHNQANGEGNRDGH
HGNHSDSCGVEGPTTDPDILARRARRQRNMLATLFLSQGTPMLLAGDEMGNSQAGNNNAYCQDNAIGWVDWQADQAGLFD
FVQQLSAFRAAHPVLRQSRFLHGAARVADGRPDVEWSDATGAAPDWHDPDLSTFCLMLRGSAEASVDTVGDDAVLVVINR
APDPVKIALPPPPTGQCWQIGMRTGAAARGKLEAGELVVEAEEVMALILSPMDAPDAW
>Mature_698_residues
MDYHFTSGNPTQLGAHHDGQGVNFAVFSAHASAIELCLFTPDGHTETARLALPERSGDIWHGYLPGLAVGTVYGYRAHGP
YAPEQGHRFNPNKLLLDPYTRQLCGAFKDDPALLGYVEGDAEEDLSFDPRDSAPYVAKSVVRDPAIFPKAGTGPQIPWEE
TLIYEAHVKGLTAQNDRVPADLRGTYDALASDAMLAHFKRLGITAVELLPVHAFVNDSFLLKKGLTNYWGYNTIGFFAPE
PRYVGPAGIAGFQKMVQRLHAAGIEVILDVVYNHTAEGDQRGPTLSFRGLDNASYYRLTDADPRFYVNDTGTGNTLNVAH
PQVLRMVLDSLRFWVEHMGVDGFRFDLATTLAREPDGFDPQGGFMDALRQDPVLGGVKLIAEPWDIGPGGYQLGAYPPEF
AEWNDAYRDTVRRFWKGDSHSAQDLAARLLGSAEIFDHRGRRAWSSLNFLAAHDGFTLADTTSYARRHNQANGEGNRDGH
HGNHSDSCGVEGPTTDPDILARRARRQRNMLATLFLSQGTPMLLAGDEMGNSQAGNNNAYCQDNAIGWVDWQADQAGLFD
FVQQLSAFRAAHPVLRQSRFLHGAARVADGRPDVEWSDATGAAPDWHDPDLSTFCLMLRGSAEASVDTVGDDAVLVVINR
APDPVKIALPPPPTGQCWQIGMRTGAAARGKLEAGELVVEAEEVMALILSPMDAPDAW

Specific function: Hydrolyzes The Alpha-1,6-Glucosidic Linkages In Glycogen Which Has First Been Partially Depolymerized By Phosphorylase. Shows Only Very Little Activity With Native Glycogen. [C]

COG id: COG1523

COG function: function code G; Type II secretory pathway, pullulanase PulA and related glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Homo sapiens, GI189458812, Length=189, Percent_Identity=28.042328042328, Blast_Score=72, Evalue=2e-12,
Organism=Escherichia coli, GI2367229, Length=649, Percent_Identity=45.7627118644068, Blast_Score=535, Evalue=1e-153,
Organism=Caenorhabditis elegans, GI17554896, Length=204, Percent_Identity=28.4313725490196, Blast_Score=67, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI32564391, Length=204, Percent_Identity=28.4313725490196, Blast_Score=67, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6320826, Length=202, Percent_Identity=26.2376237623762, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI28573410, Length=212, Percent_Identity=25.9433962264151, Blast_Score=75, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR011837
- InterPro:   IPR013783
- InterPro:   IPR014756 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02922 CBM_48 [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 76274; Mature: 76274

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDYHFTSGNPTQLGAHHDGQGVNFAVFSAHASAIELCLFTPDGHTETARLALPERSGDIW
CCCEECCCCCCEECCCCCCCCCEEEEEECCCCEEEEEEECCCCCCCEEEEECCCCCCCEE
HGYLPGLAVGTVYGYRAHGPYAPEQGHRFNPNKLLLDPYTRQLCGAFKDDPALLGYVEGD
ECCCCHHHHHHEECCCCCCCCCCCCCCCCCCCEEEECHHHHHHHCCCCCCCCEEEEECCC
AEEDLSFDPRDSAPYVAKSVVRDPAIFPKAGTGPQIPWEETLIYEAHVKGLTAQNDRVPA
CCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCHHEEEEEEHHCCCCCCCCCCCH
DLRGTYDALASDAMLAHFKRLGITAVELLPVHAFVNDSFLLKKGLTNYWGYNTIGFFAPE
HHCHHHHHHHHHHHHHHHHHHCCEEEHEEEEHHHHCCCHHHHHCCHHHCCCCCEEEECCC
PRYVGPAGIAGFQKMVQRLHAAGIEVILDVVYNHTAEGDQRGPTLSFRGLDNASYYRLTD
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCEEEEEC
ADPRFYVNDTGTGNTLNVAHPQVLRMVLDSLRFWVEHMGVDGFRFDLATTLAREPDGFDP
CCCCEEEECCCCCCEEECCCHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHCCCCCCCC
QGGFMDALRQDPVLGGVKLIAEPWDIGPGGYQLGAYPPEFAEWNDAYRDTVRRFWKGDSH
CCHHHHHHHCCCCCCCEEEEECCCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHCCCCC
SAQDLAARLLGSAEIFDHRGRRAWSSLNFLAAHDGFTLADTTSYARRHNQANGEGNRDGH
CHHHHHHHHHCCHHHHHHCCHHHHHCCCEEEECCCEEEEHHHHHHHHCCCCCCCCCCCCC
HGNHSDSCGVEGPTTDPDILARRARRQRNMLATLFLSQGTPMLLAGDEMGNSQAGNNNAY
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHEEEEEECCCCCEEEECCCCCCCCCCCCCCE
CQDNAIGWVDWQADQAGLFDFVQQLSAFRAAHPVLRQSRFLHGAARVADGRPDVEWSDAT
ECCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
GAAPDWHDPDLSTFCLMLRGSAEASVDTVGDDAVLVVINRAPDPVKIALPPPPTGQCWQI
CCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCEEEEECCCCCCCCEEE
GMRTGAAARGKLEAGELVVEAEEVMALILSPMDAPDAW
CCCCCCCCCCCCCCCCEEEEHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MDYHFTSGNPTQLGAHHDGQGVNFAVFSAHASAIELCLFTPDGHTETARLALPERSGDIW
CCCEECCCCCCEECCCCCCCCCEEEEEECCCCEEEEEEECCCCCCCEEEEECCCCCCCEE
HGYLPGLAVGTVYGYRAHGPYAPEQGHRFNPNKLLLDPYTRQLCGAFKDDPALLGYVEGD
ECCCCHHHHHHEECCCCCCCCCCCCCCCCCCCEEEECHHHHHHHCCCCCCCCEEEEECCC
AEEDLSFDPRDSAPYVAKSVVRDPAIFPKAGTGPQIPWEETLIYEAHVKGLTAQNDRVPA
CCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCHHEEEEEEHHCCCCCCCCCCCH
DLRGTYDALASDAMLAHFKRLGITAVELLPVHAFVNDSFLLKKGLTNYWGYNTIGFFAPE
HHCHHHHHHHHHHHHHHHHHHCCEEEHEEEEHHHHCCCHHHHHCCHHHCCCCCEEEECCC
PRYVGPAGIAGFQKMVQRLHAAGIEVILDVVYNHTAEGDQRGPTLSFRGLDNASYYRLTD
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCEEEEEC
ADPRFYVNDTGTGNTLNVAHPQVLRMVLDSLRFWVEHMGVDGFRFDLATTLAREPDGFDP
CCCCEEEECCCCCCEEECCCHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHCCCCCCCC
QGGFMDALRQDPVLGGVKLIAEPWDIGPGGYQLGAYPPEFAEWNDAYRDTVRRFWKGDSH
CCHHHHHHHCCCCCCCEEEEECCCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHCCCCC
SAQDLAARLLGSAEIFDHRGRRAWSSLNFLAAHDGFTLADTTSYARRHNQANGEGNRDGH
CHHHHHHHHHCCHHHHHHCCHHHHHCCCEEEECCCEEEEHHHHHHHHCCCCCCCCCCCCC
HGNHSDSCGVEGPTTDPDILARRARRQRNMLATLFLSQGTPMLLAGDEMGNSQAGNNNAY
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHEEEEEECCCCCEEEECCCCCCCCCCCCCCE
CQDNAIGWVDWQADQAGLFDFVQQLSAFRAAHPVLRQSRFLHGAARVADGRPDVEWSDAT
ECCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
GAAPDWHDPDLSTFCLMLRGSAEASVDTVGDDAVLVVINRAPDPVKIALPPPPTGQCWQI
CCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCEEEEECCCCCCCCEEE
GMRTGAAARGKLEAGELVVEAEEVMALILSPMDAPDAW
CCCCCCCCCCCCCCCCEEEEHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Glycosylases; Glycosidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]