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The map label for this gene is braE [H]

Identifier: 110680050

GI number: 110680050

Start: 2715840

End: 2717039

Strand: Direct

Name: braE [H]

Synonym: RD1_2845

Alternate gene names: 110680050

Gene position: 2715840-2717039 (Clockwise)

Preceding gene: 110680049

Following gene: 110680051

Centisome position: 65.71

GC content: 58.25

Gene sequence:

>1200_bases
ATGTTCGGACTTACAAAGAAAGACACGACGCTGCTGTTGATCGTCGCCTGTCTCACCATGTTCGCCCCCTTCATTCTGAA
CCCCTTCCCCGAAGGCTCCGCGATGGCGCAGTTCAACGCGGGCTATCCGGACCTGATGCAACGGCTGGTGATCTTTGGCA
TCTTTGCCATCGGGTTCAATATCCTCTTTGGCCTCACGGGCTACCTCAGCTTTGGCCATGCGGCGTTTCTCGGTGTCGGC
TCCTATGCGGCGATCTGGATGATGAAGCTGCTGACCATGAACGTGATCCCGGCGATCATGGTCTCTGTGCTGATCGCCGG
TCTGTTTTCGCTGCTGGTGGGGTGGATATCGCTGCGCCGCTCCGGCATCTACTTTTCGATCCTGACGCTGGCCTTCGCGC
AGATGTCCTATTCGCTGGCCTATTCGGTGCTGACGCCGATCACCGGTGGTGAAACCGGGCTGCAGCCCAAGGTCAACGAC
CCGCGCATCCTTGATAGCGCGCTGGAGCCCGGTCAGAACCCGCGCGCCAACCTCTTCGGTCTGGAAATGAACCAAAGCTA
TGAACTGGCGATGGGCGGCTGGGTCTTTACCTTCAACGCCGGGTATTACATCGCGGCCCTTGTCATGCTGCTGGCGTTCT
ACCTGTCGATCCGCATCTTCCGCTCGCCTTTCGGGATGATGCTGCGGGCGGTCAAATCCAACCAGCAACGGCTGAACTAC
ACCGGCCTGAACGCAAAACCCTACACGCTGGCGGCTTTTGTCATCTCCGGCATGTATGCGGGGCTGGCGGGCGGATTGCT
GGTGGCGATGGATACGCAGGTGGGCGCGGAACGCATGTTCTGGACAGCTTCCGGCGAGGTGGTTCTGATGACCATTCTCG
GCGGTGCCGGGACGTTGATCGGGCCGGTCCTTGGTGCAGGGTTCATCAAATATATGGAAAACATCGTCTCCAAGATCAAC
AAGACCGTTCTGGAGCAGTGGTTTGCCTTCATGCCTGACGGGATCGAGGATTTCATCATCACGATCGTCTACCCCTTTGT
GGGCAAAGGCTGGCACCTCACGCTTGGGCTGCTCTTCATGATGGTGGTGATCTTCCTGCCCGGTGGTCTTGTTCAGGGCG
GTCAGAAAGTCGCGGGTCTGTTCCGGCGCAAGAAACCCGCCGATGACAAGCCTGATGGCAAAACAACACCTGCGGAATAA

Upstream 100 bases:

>100_bases
GGCATCAACCAAATCATCATCTATCTGGTCGCAATCATCATCTTGCTTACCCGTCCACGCGGACTCATGGGGCGCAAGGG
CGTGATGGAGGAATAATCAC

Downstream 100 bases:

>100_bases
GGAGACGACGTTATGGGAATTCTCGAAGTCAAAAACGTCAACAAGCGGTTCGGGGGCCTGCAAGCCCTCGGCGACGTGAA
CCTCTCCATCGCAGAGCGGT

Product: branched-chain amino acid ABC transporter, permease protein, putative

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 399; Mature: 399

Protein sequence:

>399_residues
MFGLTKKDTTLLLIVACLTMFAPFILNPFPEGSAMAQFNAGYPDLMQRLVIFGIFAIGFNILFGLTGYLSFGHAAFLGVG
SYAAIWMMKLLTMNVIPAIMVSVLIAGLFSLLVGWISLRRSGIYFSILTLAFAQMSYSLAYSVLTPITGGETGLQPKVND
PRILDSALEPGQNPRANLFGLEMNQSYELAMGGWVFTFNAGYYIAALVMLLAFYLSIRIFRSPFGMMLRAVKSNQQRLNY
TGLNAKPYTLAAFVISGMYAGLAGGLLVAMDTQVGAERMFWTASGEVVLMTILGGAGTLIGPVLGAGFIKYMENIVSKIN
KTVLEQWFAFMPDGIEDFIITIVYPFVGKGWHLTLGLLFMMVVIFLPGGLVQGGQKVAGLFRRKKPADDKPDGKTTPAE

Sequences:

>Translated_399_residues
MFGLTKKDTTLLLIVACLTMFAPFILNPFPEGSAMAQFNAGYPDLMQRLVIFGIFAIGFNILFGLTGYLSFGHAAFLGVG
SYAAIWMMKLLTMNVIPAIMVSVLIAGLFSLLVGWISLRRSGIYFSILTLAFAQMSYSLAYSVLTPITGGETGLQPKVND
PRILDSALEPGQNPRANLFGLEMNQSYELAMGGWVFTFNAGYYIAALVMLLAFYLSIRIFRSPFGMMLRAVKSNQQRLNY
TGLNAKPYTLAAFVISGMYAGLAGGLLVAMDTQVGAERMFWTASGEVVLMTILGGAGTLIGPVLGAGFIKYMENIVSKIN
KTVLEQWFAFMPDGIEDFIITIVYPFVGKGWHLTLGLLFMMVVIFLPGGLVQGGQKVAGLFRRKKPADDKPDGKTTPAE
>Mature_399_residues
MFGLTKKDTTLLLIVACLTMFAPFILNPFPEGSAMAQFNAGYPDLMQRLVIFGIFAIGFNILFGLTGYLSFGHAAFLGVG
SYAAIWMMKLLTMNVIPAIMVSVLIAGLFSLLVGWISLRRSGIYFSILTLAFAQMSYSLAYSVLTPITGGETGLQPKVND
PRILDSALEPGQNPRANLFGLEMNQSYELAMGGWVFTFNAGYYIAALVMLLAFYLSIRIFRSPFGMMLRAVKSNQQRLNY
TGLNAKPYTLAAFVISGMYAGLAGGLLVAMDTQVGAERMFWTASGEVVLMTILGGAGTLIGPVLGAGFIKYMENIVSKIN
KTVLEQWFAFMPDGIEDFIITIVYPFVGKGWHLTLGLLFMMVVIFLPGGLVQGGQKVAGLFRRKKPADDKPDGKTTPAE

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG4177

COG function: function code E; ABC-type branched-chain amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=296, Percent_Identity=26.0135135135135, Blast_Score=82, Evalue=6e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 43430; Mature: 43430

Theoretical pI: Translated: 9.56; Mature: 9.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
5.5 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
5.5 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFGLTKKDTTLLLIVACLTMFAPFILNPFPEGSAMAQFNAGYPDLMQRLVIFGIFAIGFN
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHH
ILFGLTGYLSFGHAAFLGVGSYAAIWMMKLLTMNVIPAIMVSVLIAGLFSLLVGWISLRR
HHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SGIYFSILTLAFAQMSYSLAYSVLTPITGGETGLQPKVNDPRILDSALEPGQNPRANLFG
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCEEE
LEMNQSYELAMGGWVFTFNAGYYIAALVMLLAFYLSIRIFRSPFGMMLRAVKSNQQRLNY
EECCCCEEEEECCEEEEECCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHCCC
TGLNAKPYTLAAFVISGMYAGLAGGLLVAMDTQVGAERMFWTASGEVVLMTILGGAGTLI
CCCCCCCHHHHHHHHHHHHHHHHCCEEEEEECCCCCHHEEEECCCCEEEEEEECCCHHHH
GPVLGAGFIKYMENIVSKINKTVLEQWFAFMPDGIEDFIITIVYPFVGKGWHLTLGLLFM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHH
MVVIFLPGGLVQGGQKVAGLFRRKKPADDKPDGKTTPAE
HHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MFGLTKKDTTLLLIVACLTMFAPFILNPFPEGSAMAQFNAGYPDLMQRLVIFGIFAIGFN
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHH
ILFGLTGYLSFGHAAFLGVGSYAAIWMMKLLTMNVIPAIMVSVLIAGLFSLLVGWISLRR
HHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SGIYFSILTLAFAQMSYSLAYSVLTPITGGETGLQPKVNDPRILDSALEPGQNPRANLFG
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCEEE
LEMNQSYELAMGGWVFTFNAGYYIAALVMLLAFYLSIRIFRSPFGMMLRAVKSNQQRLNY
EECCCCEEEEECCEEEEECCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHCCC
TGLNAKPYTLAAFVISGMYAGLAGGLLVAMDTQVGAERMFWTASGEVVLMTILGGAGTLI
CCCCCCCHHHHHHHHHHHHHHHHCCEEEEEECCCCCHHEEEECCCCEEEEEEECCCHHHH
GPVLGAGFIKYMENIVSKINKTVLEQWFAFMPDGIEDFIITIVYPFVGKGWHLTLGLLFM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHH
MVVIFLPGGLVQGGQKVAGLFRRKKPADDKPDGKTTPAE
HHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]