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The map label for this gene is rlmI [H]

Identifier: 110680008

GI number: 110680008

Start: 2667016

End: 2668239

Strand: Reverse

Name: rlmI [H]

Synonym: RD1_2799

Alternate gene names: 110680008

Gene position: 2668239-2667016 (Counterclockwise)

Preceding gene: 110680009

Following gene: 110680007

Centisome position: 64.56

GC content: 63.32

Gene sequence:

>1224_bases
ATGACAGCATCCATTCCTGAAAAAGCAGCCCCCGCCGGTGATCTGCCCACCGTCCGGCTGAAACCGAAAGCCAATGCGCG
CGCGATCCGCCATGGGTTCCCTTGGGTCTATGCCAATGAGTTGGTCACCGACCGGCGGACCCGCTCGAAACCCGCCGGAA
CCCTCGCGGTTCTGGAGGATGACGGGCGTGTTCCTCTGGGTCTTGTGGCGCTTAATCCGGACTCCAAGATCATCTGCCGG
ATGCTGGACCGCGACCCGCAGGCCAGCATTGACGCGGCATGGTTCGAGGCACGGTTGCGCAAGGCGCTTCACTTGCGTGA
ACGGGTGTTTGACACCCCCTATTACAGACTCGCCCATGCAGAGGCGGACGGGCTGCCGGGCGTGGTGATTGATCGCTTTG
GCGACGCTTGTGTCGTGCAGCCGAATGCCGCATGGGCCGAGGCTTTTTTGCCTGCGCTGACGGATGCTCTCGTCGCCGTG
ACCGGGGTGCGCGACGTTCTGAAAAATGCCAGCGGGCGTACAAGGTCGCTGGAAGGGCTGGACGATGTGAATGCTGTTCT
GCATGGCAGCGCCCCCGACGCGCCGATCCCGGTGTCGATGAATGGCGCGGTTTATATGGCCGATGTGACGGGCGGGCAGA
AAACCGGTCTTTATTTTGATCAACGGCCAAATCATGCTTTTGCCGCGTCGCTGGCGCGGGATGCGCGGGTCTTGGATGTC
TTCAGCCACGTTGGTGGTTTTTCGCTGGCCGCACTCGCCGGGGGTGCGCAAAGCGCGCTGGCCGTGGATGGATCCGCCGC
CGCATTGACGCTGGCCGAAGCGGGGGCCAAGGCGTCGGGGTGCGCGGATCGGTTCCAGACCCGTCAGGGCGATGCCTTTG
AGGTGCTCGCCGCATTGGCGCAGGAGGGCGCGCAGTTTGATCTGGTGATTTGCGATCCGCCTGCCTTTGCCCCGTCCAAG
CAAGCGTTGGATGCGGGGCTGCGCGCCTATGAACGTGTCGCGCGACTGGCAGCGACGCTGGTGGCGGAAGATGGCATTCT
TGGCCTGTGTTCCTGTTCGCATGCGGCTGATCTGTCGGCGTTTCGTGCGGCCTGCGCGCGCGGGATCGGCCGTTCGGGCC
GGCGCGGTTCGCTGATATACACGGGATTTGCAGGACCGGATCATCCGCAGCTGCCACAGCTGGCCGAAAGCGGATATCTG
AAGTCCGTGTTCTTTCAACTGTGA

Upstream 100 bases:

>100_bases
AACTGAATTCCAACGCAGCGCTGGTGGCACCGGCGCCGTGGCCGCATCCTTTCCATTCGCAGCTTCCCTCTGGCGCGCGA
AGGCAGTAGAGCAGGGCAGT

Downstream 100 bases:

>100_bases
TGTGATGAAAATCCTTATTGATACCTGCGTGCTTTATCCCACGGTGATGCGCGAAATGGTGTTGCAAACGGCGTGCGCGG
GGGCCTTCACCCCGCTCTGG

Product: hypothetical protein

Products: NA

Alternate protein names: 23S rRNA m5C1962 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase RlmI [H]

Number of amino acids: Translated: 407; Mature: 406

Protein sequence:

>407_residues
MTASIPEKAAPAGDLPTVRLKPKANARAIRHGFPWVYANELVTDRRTRSKPAGTLAVLEDDGRVPLGLVALNPDSKIICR
MLDRDPQASIDAAWFEARLRKALHLRERVFDTPYYRLAHAEADGLPGVVIDRFGDACVVQPNAAWAEAFLPALTDALVAV
TGVRDVLKNASGRTRSLEGLDDVNAVLHGSAPDAPIPVSMNGAVYMADVTGGQKTGLYFDQRPNHAFAASLARDARVLDV
FSHVGGFSLAALAGGAQSALAVDGSAAALTLAEAGAKASGCADRFQTRQGDAFEVLAALAQEGAQFDLVICDPPAFAPSK
QALDAGLRAYERVARLAATLVAEDGILGLCSCSHAADLSAFRAACARGIGRSGRRGSLIYTGFAGPDHPQLPQLAESGYL
KSVFFQL

Sequences:

>Translated_407_residues
MTASIPEKAAPAGDLPTVRLKPKANARAIRHGFPWVYANELVTDRRTRSKPAGTLAVLEDDGRVPLGLVALNPDSKIICR
MLDRDPQASIDAAWFEARLRKALHLRERVFDTPYYRLAHAEADGLPGVVIDRFGDACVVQPNAAWAEAFLPALTDALVAV
TGVRDVLKNASGRTRSLEGLDDVNAVLHGSAPDAPIPVSMNGAVYMADVTGGQKTGLYFDQRPNHAFAASLARDARVLDV
FSHVGGFSLAALAGGAQSALAVDGSAAALTLAEAGAKASGCADRFQTRQGDAFEVLAALAQEGAQFDLVICDPPAFAPSK
QALDAGLRAYERVARLAATLVAEDGILGLCSCSHAADLSAFRAACARGIGRSGRRGSLIYTGFAGPDHPQLPQLAESGYL
KSVFFQL
>Mature_406_residues
TASIPEKAAPAGDLPTVRLKPKANARAIRHGFPWVYANELVTDRRTRSKPAGTLAVLEDDGRVPLGLVALNPDSKIICRM
LDRDPQASIDAAWFEARLRKALHLRERVFDTPYYRLAHAEADGLPGVVIDRFGDACVVQPNAAWAEAFLPALTDALVAVT
GVRDVLKNASGRTRSLEGLDDVNAVLHGSAPDAPIPVSMNGAVYMADVTGGQKTGLYFDQRPNHAFAASLARDARVLDVF
SHVGGFSLAALAGGAQSALAVDGSAAALTLAEAGAKASGCADRFQTRQGDAFEVLAALAQEGAQFDLVICDPPAFAPSKQ
ALDAGLRAYERVARLAATLVAEDGILGLCSCSHAADLSAFRAACARGIGRSGRRGSLIYTGFAGPDHPQLPQLAESGYLK
SVFFQL

Specific function: Specifically methylates the cytosine at position 1962 (m5C1962) of 23S rRNA [H]

COG id: COG1092

COG function: function code R; Predicted SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Escherichia coli, GI145693111, Length=385, Percent_Identity=30.9090909090909, Blast_Score=177, Evalue=2e-45,
Organism=Escherichia coli, GI1787181, Length=251, Percent_Identity=29.0836653386454, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002478
- InterPro:   IPR015947
- InterPro:   IPR019614 [H]

Pfam domain/function: PF10672 Methyltrans_SAM [H]

EC number: =2.1.1.191 [H]

Molecular weight: Translated: 42840; Mature: 42709

Theoretical pI: Translated: 6.93; Mature: 6.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTASIPEKAAPAGDLPTVRLKPKANARAIRHGFPWVYANELVTDRRTRSKPAGTLAVLED
CCCCCCCCCCCCCCCCEEEECCCCCCHHHHCCCCEEEHHHHHHHHHHCCCCCCEEEEEEC
DGRVPLGLVALNPDSKIICRMLDRDPQASIDAAWFEARLRKALHLRERVFDTPYYRLAHA
CCCCEEEEEEECCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHEEEEC
EADGLPGVVIDRFGDACVVQPNAAWAEAFLPALTDALVAVTGVRDVLKNASGRTRSLEGL
CCCCCCCCEEECCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
DDVNAVLHGSAPDAPIPVSMNGAVYMADVTGGQKTGLYFDQRPNHAFAASLARDARVLDV
HHHHHHEECCCCCCCCEEECCCEEEEEEECCCCCCCEEECCCCCHHHHHHHHHHHHHHHH
FSHVGGFSLAALAGGAQSALAVDGSAAALTLAEAGAKASGCADRFQTRQGDAFEVLAALA
HHHCCCHHHHHHCCCCCCEEEECCCHHEEEHHHCCCCCCCHHHHHHCCCCCHHHHHHHHH
QEGAQFDLVICDPPAFAPSKQALDAGLRAYERVARLAATLVAEDGILGLCSCSHAADLSA
HCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHH
FRAACARGIGRSGRRGSLIYTGFAGPDHPQLPQLAESGYLKSVFFQL
HHHHHHHHCCCCCCCCCEEEEECCCCCCCCCHHHHHCCCHHHHHHCC
>Mature Secondary Structure 
TASIPEKAAPAGDLPTVRLKPKANARAIRHGFPWVYANELVTDRRTRSKPAGTLAVLED
CCCCCCCCCCCCCCCEEEECCCCCCHHHHCCCCEEEHHHHHHHHHHCCCCCCEEEEEEC
DGRVPLGLVALNPDSKIICRMLDRDPQASIDAAWFEARLRKALHLRERVFDTPYYRLAHA
CCCCEEEEEEECCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHEEEEC
EADGLPGVVIDRFGDACVVQPNAAWAEAFLPALTDALVAVTGVRDVLKNASGRTRSLEGL
CCCCCCCCEEECCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
DDVNAVLHGSAPDAPIPVSMNGAVYMADVTGGQKTGLYFDQRPNHAFAASLARDARVLDV
HHHHHHEECCCCCCCCEEECCCEEEEEEECCCCCCCEEECCCCCHHHHHHHHHHHHHHHH
FSHVGGFSLAALAGGAQSALAVDGSAAALTLAEAGAKASGCADRFQTRQGDAFEVLAALA
HHHCCCHHHHHHCCCCCCEEEECCCHHEEEHHHCCCCCCCHHHHHHCCCCCHHHHHHHHH
QEGAQFDLVICDPPAFAPSKQALDAGLRAYERVARLAATLVAEDGILGLCSCSHAADLSA
HCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHH
FRAACARGIGRSGRRGSLIYTGFAGPDHPQLPQLAESGYLKSVFFQL
HHHHHHHHCCCCCCCCCEEEEECCCCCCCCCHHHHHCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA