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The map label for this gene is lipA [H]

Identifier: 110679983

GI number: 110679983

Start: 2643393

End: 2644346

Strand: Reverse

Name: lipA [H]

Synonym: RD1_2773

Alternate gene names: 110679983

Gene position: 2644346-2643393 (Counterclockwise)

Preceding gene: 110679984

Following gene: 110679982

Centisome position: 63.98

GC content: 58.49

Gene sequence:

>954_bases
ATGCGTGACATTAAAATTCCCGAGCAGCGCCATCCGGAAAAACAGCGCCGCGCCGACAGCCCACAGCCAAAAAAACCCGA
CTGGATTCGCGTGAAGGCCCCCGGTGGCAAGGGCTATGCGGAAACTGCGAAGATCATGCGGGAAAACAAGCTTGTCACGG
TCTGCGAAGAGGCCGGCTGCCCCAATTTGGGTGAATGCTGGAGCCAGGGCCACGCGACCATGATGATCATGGGCGAGGTT
TGCACCCGTGCCTGCACGTTTTGTAATATCGCAACAGGCAAACCCCCCGAAGATCTGGATGCATTCGAACCGGGCCGCGT
CGCCCATGCGGTCGAAAAGCTGGGGTTGAACCATGTGGTCATCACCTCGGTTGATCGCGATGATGTCGAAGATGGCGGTG
CCGATCATTTTGCACAGACGATTCGGGCCATACGTCACCGTAGCCCCGCCACCACCATCGAAATCCTGACACCGGATTTC
ATCAAATGTGCGCCCGAGGCGCTGGAAACGGTCGTCGCGGCGCGACCGGATGTCTTTAACCACAACCTCGAAACCGTGCC
GGGGCTTTACCCGGAAGTGCGCCCGGGCGCGCGCTACTTCCATTCGCTGCGCCTGCTGCAACGGGTGAAAGAGCTTGACC
CGCGCATGTTTACCAAATCCGGTATCATGGTGGGCCTGGGCGAAGACCGGCAATCCGTGATGCAGGTCATGGATGATATG
CGCGCCGCTGACATAGATTTTTTGACGATCGGTCAGTACCTTCAACCGACGCCGAAACATCACCGTGTTGATCGCTTCGT
GACGCCGGATGAATTTGCATCCTACGAAAAGGCGGCATTTGGCAAAGGGTTCCTGATGGTGTCAGCAACGCCGCTGACAC
GCTCTTCCTATCACGCGGGGGATGATTTTGCGCGTTTGCGTGCCGCACGCGAGGCGCGGCTCAGTGCCGGATGA

Upstream 100 bases:

>100_bases
TGACGATATCGCCTGAGGAATAGCGGCCATGCGTCGCGGGACCTTTCGCTGAAACGTCGGTGTGATACACAAGGGGCAGA
CAACCACTCTGGAACAGCAG

Downstream 100 bases:

>100_bases
AAATCGTGTCAAAAATGTGCTGCTTCCATAAAAATGTGCTTGAGCACTTTACACAACCTTAACGATAGTTGAGTGTGGCA
CAAAATTTCACCCTTTCTAC

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA [H]

Number of amino acids: Translated: 317; Mature: 317

Protein sequence:

>317_residues
MRDIKIPEQRHPEKQRRADSPQPKKPDWIRVKAPGGKGYAETAKIMRENKLVTVCEEAGCPNLGECWSQGHATMMIMGEV
CTRACTFCNIATGKPPEDLDAFEPGRVAHAVEKLGLNHVVITSVDRDDVEDGGADHFAQTIRAIRHRSPATTIEILTPDF
IKCAPEALETVVAARPDVFNHNLETVPGLYPEVRPGARYFHSLRLLQRVKELDPRMFTKSGIMVGLGEDRQSVMQVMDDM
RAADIDFLTIGQYLQPTPKHHRVDRFVTPDEFASYEKAAFGKGFLMVSATPLTRSSYHAGDDFARLRAAREARLSAG

Sequences:

>Translated_317_residues
MRDIKIPEQRHPEKQRRADSPQPKKPDWIRVKAPGGKGYAETAKIMRENKLVTVCEEAGCPNLGECWSQGHATMMIMGEV
CTRACTFCNIATGKPPEDLDAFEPGRVAHAVEKLGLNHVVITSVDRDDVEDGGADHFAQTIRAIRHRSPATTIEILTPDF
IKCAPEALETVVAARPDVFNHNLETVPGLYPEVRPGARYFHSLRLLQRVKELDPRMFTKSGIMVGLGEDRQSVMQVMDDM
RAADIDFLTIGQYLQPTPKHHRVDRFVTPDEFASYEKAAFGKGFLMVSATPLTRSSYHAGDDFARLRAAREARLSAG
>Mature_317_residues
MRDIKIPEQRHPEKQRRADSPQPKKPDWIRVKAPGGKGYAETAKIMRENKLVTVCEEAGCPNLGECWSQGHATMMIMGEV
CTRACTFCNIATGKPPEDLDAFEPGRVAHAVEKLGLNHVVITSVDRDDVEDGGADHFAQTIRAIRHRSPATTIEILTPDF
IKCAPEALETVVAARPDVFNHNLETVPGLYPEVRPGARYFHSLRLLQRVKELDPRMFTKSGIMVGLGEDRQSVMQVMDDM
RAADIDFLTIGQYLQPTPKHHRVDRFVTPDEFASYEKAAFGKGFLMVSATPLTRSSYHAGDDFARLRAAREARLSAG

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives [H]

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family [H]

Homologues:

Organism=Homo sapiens, GI37577166, Length=305, Percent_Identity=43.6065573770492, Blast_Score=269, Evalue=2e-72,
Organism=Homo sapiens, GI37577164, Length=255, Percent_Identity=44.7058823529412, Blast_Score=234, Evalue=6e-62,
Organism=Escherichia coli, GI1786846, Length=277, Percent_Identity=50.5415162454874, Blast_Score=285, Evalue=4e-78,
Organism=Caenorhabditis elegans, GI32564533, Length=259, Percent_Identity=44.4015444015444, Blast_Score=239, Evalue=1e-63,
Organism=Saccharomyces cerevisiae, GI6324770, Length=297, Percent_Identity=41.7508417508418, Blast_Score=255, Evalue=8e-69,
Organism=Drosophila melanogaster, GI221513272, Length=296, Percent_Identity=43.2432432432432, Blast_Score=265, Evalue=3e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.8.1.8 [H]

Molecular weight: Translated: 35337; Mature: 35337

Theoretical pI: Translated: 7.31; Mature: 7.31

Prosite motif: PS00228 TUBULIN_B_AUTOREG

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRDIKIPEQRHPEKQRRADSPQPKKPDWIRVKAPGGKGYAETAKIMRENKLVTVCEEAGC
CCCCCCCHHCCCHHHHCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHCCCEEEEEHHCCC
PNLGECWSQGHATMMIMGEVCTRACTFCNIATGKPPEDLDAFEPGRVAHAVEKLGLNHVV
CCHHHHHCCCCEEEEEHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEE
ITSVDRDDVEDGGADHFAQTIRAIRHRSPATTIEILTPDFIKCAPEALETVVAARPDVFN
EEECCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCHHHHHCHHHHHHHHHCCCCHHC
HNLETVPGLYPEVRPGARYFHSLRLLQRVKELDPRMFTKSGIMVGLGEDRQSVMQVMDDM
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHCCCEEEECCCCHHHHHHHHHHH
RAADIDFLTIGQYLQPTPKHHRVDRFVTPDEFASYEKAAFGKGFLMVSATPLTRSSYHAG
HHHCCCCEEHHHHHCCCCCHHCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCC
DDFARLRAAREARLSAG
HHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRDIKIPEQRHPEKQRRADSPQPKKPDWIRVKAPGGKGYAETAKIMRENKLVTVCEEAGC
CCCCCCCHHCCCHHHHCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHCCCEEEEEHHCCC
PNLGECWSQGHATMMIMGEVCTRACTFCNIATGKPPEDLDAFEPGRVAHAVEKLGLNHVV
CCHHHHHCCCCEEEEEHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEE
ITSVDRDDVEDGGADHFAQTIRAIRHRSPATTIEILTPDFIKCAPEALETVVAARPDVFN
EEECCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCHHHHHCHHHHHHHHHCCCCHHC
HNLETVPGLYPEVRPGARYFHSLRLLQRVKELDPRMFTKSGIMVGLGEDRQSVMQVMDDM
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHCCCEEEECCCCHHHHHHHHHHH
RAADIDFLTIGQYLQPTPKHHRVDRFVTPDEFASYEKAAFGKGFLMVSATPLTRSSYHAG
HHHCCCCEEHHHHHCCCCCHHCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCC
DDFARLRAAREARLSAG
HHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA