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The map label for this gene is phoB [H]

Identifier: 110679874

GI number: 110679874

Start: 2527774

End: 2528463

Strand: Direct

Name: phoB [H]

Synonym: RD1_2648

Alternate gene names: 110679874

Gene position: 2527774-2528463 (Clockwise)

Preceding gene: 110679873

Following gene: 110679876

Centisome position: 61.16

GC content: 58.84

Gene sequence:

>690_bases
ATGTCGGCTGATCAACCTCTTGTTTTAGTTGTCGAAGATGAGCCTGCGCAGCGCGAGGTGCTGGCCTATAATCTGGAGGC
TGAAGGCTTTGCCGTCAGCCGCGCGGAAAACGGCGAAGAAGCGATGATGCTGGTGGATGAAACAGCACCTGATCTGATCG
TGCTGGATTGGATGATGCCAAACCTGTCGGGCATCGAGGTGTGTCGTCGGTTGAAGACAAGGTCTGAAACCCGCGCGATT
CCGATCATCATGCTGTCTGCGCGCTCGGAGGAAGTGGATCGCGTGCGTGGACTGGAGACAGGCGCGGATGACTATGTGAT
CAAACCCTATTCCATCGTCGAACTGATGGCGCGGGTGCGCAGCCAGTTGCGCCGCGTGCGACCGGCGGCCACCGGCGGGC
AGTTGGAATATGAGGATATTGTTCTGAATGCCGCAAGCCATCGCGTGAGCCGCGGCGAAAACGAACTGAAACTTGGCCCG
ACCGAGTTTCGCCTCTTGTCCACCTTCATGGAAAAACCGGGCCGTGTGTTTTCACGCGAACAATTGCTCGACCGTGTGTG
GGGTCGCGATATTTACGTGGATACGCGCACCGTGGACGTTCATATCGGACGGTTGCGCAAGGCGCTGACACAGTTTGGCG
GTTCGGATCCGCTGCGCACGGTGCGCGGCGCGGGATACGCGCTGGGGTAG

Upstream 100 bases:

>100_bases
CGATCGCCGAACAAGTTGTGTATCTTGTGACCGGCGAGCATCCGGAAGATCCGCGCCCAAAGGCGGATCAAACCTCCGTG
GTCGCCAAGGGGTAAGGCGG

Downstream 100 bases:

>100_bases
GGACGTCAGACGCGCGTCGGTCTCAGCCCGAGAATGGCGCGCGCTTCGGCGCAGGTCGCGACGGGGCGTTCGTATTTTTC
ACATAATTCAGCGGCCCGTT

Product: phosphate regulon transcriptional regulatory protein PhoB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 229; Mature: 228

Protein sequence:

>229_residues
MSADQPLVLVVEDEPAQREVLAYNLEAEGFAVSRAENGEEAMMLVDETAPDLIVLDWMMPNLSGIEVCRRLKTRSETRAI
PIIMLSARSEEVDRVRGLETGADDYVIKPYSIVELMARVRSQLRRVRPAATGGQLEYEDIVLNAASHRVSRGENELKLGP
TEFRLLSTFMEKPGRVFSREQLLDRVWGRDIYVDTRTVDVHIGRLRKALTQFGGSDPLRTVRGAGYALG

Sequences:

>Translated_229_residues
MSADQPLVLVVEDEPAQREVLAYNLEAEGFAVSRAENGEEAMMLVDETAPDLIVLDWMMPNLSGIEVCRRLKTRSETRAI
PIIMLSARSEEVDRVRGLETGADDYVIKPYSIVELMARVRSQLRRVRPAATGGQLEYEDIVLNAASHRVSRGENELKLGP
TEFRLLSTFMEKPGRVFSREQLLDRVWGRDIYVDTRTVDVHIGRLRKALTQFGGSDPLRTVRGAGYALG
>Mature_228_residues
SADQPLVLVVEDEPAQREVLAYNLEAEGFAVSRAENGEEAMMLVDETAPDLIVLDWMMPNLSGIEVCRRLKTRSETRAIP
IIMLSARSEEVDRVRGLETGADDYVIKPYSIVELMARVRSQLRRVRPAATGGQLEYEDIVLNAASHRVSRGENELKLGPT
EFRLLSTFMEKPGRVFSREQLLDRVWGRDIYVDTRTVDVHIGRLRKALTQFGGSDPLRTVRGAGYALG

Specific function: This protein is a positive regulator for the phosphate regulon. Transcription of this operon is positively regulated by phoB and phoR when phosphate is limited [H]

COG id: COG0745

COG function: function code TK; Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=219, Percent_Identity=53.4246575342466, Blast_Score=234, Evalue=5e-63,
Organism=Escherichia coli, GI1788394, Length=228, Percent_Identity=39.9122807017544, Blast_Score=166, Evalue=2e-42,
Organism=Escherichia coli, GI1790860, Length=225, Percent_Identity=43.5555555555556, Blast_Score=162, Evalue=1e-41,
Organism=Escherichia coli, GI1786911, Length=225, Percent_Identity=40, Blast_Score=151, Evalue=3e-38,
Organism=Escherichia coli, GI1786784, Length=221, Percent_Identity=37.10407239819, Blast_Score=147, Evalue=6e-37,
Organism=Escherichia coli, GI87082012, Length=221, Percent_Identity=34.841628959276, Blast_Score=138, Evalue=3e-34,
Organism=Escherichia coli, GI1789809, Length=232, Percent_Identity=35.7758620689655, Blast_Score=134, Evalue=5e-33,
Organism=Escherichia coli, GI1787229, Length=229, Percent_Identity=35.3711790393013, Blast_Score=125, Evalue=2e-30,
Organism=Escherichia coli, GI1790863, Length=231, Percent_Identity=33.7662337662338, Blast_Score=121, Evalue=4e-29,
Organism=Escherichia coli, GI2367329, Length=229, Percent_Identity=38.8646288209607, Blast_Score=120, Evalue=7e-29,
Organism=Escherichia coli, GI1789402, Length=226, Percent_Identity=32.3008849557522, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1790552, Length=221, Percent_Identity=33.9366515837104, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1787375, Length=219, Percent_Identity=32.4200913242009, Blast_Score=115, Evalue=2e-27,
Organism=Escherichia coli, GI145693140, Length=232, Percent_Identity=26.2931034482759, Blast_Score=80, Evalue=8e-17,
Organism=Escherichia coli, GI1788550, Length=117, Percent_Identity=35.042735042735, Blast_Score=74, Evalue=6e-15,
Organism=Escherichia coli, GI1790437, Length=141, Percent_Identity=31.2056737588652, Blast_Score=65, Evalue=3e-12,
Organism=Escherichia coli, GI1788191, Length=126, Percent_Identity=26.984126984127, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1790299, Length=113, Percent_Identity=31.858407079646, Blast_Score=64, Evalue=7e-12,
Organism=Escherichia coli, GI1788713, Length=145, Percent_Identity=31.0344827586207, Blast_Score=64, Evalue=9e-12,
Organism=Escherichia coli, GI145693157, Length=109, Percent_Identity=29.3577981651376, Blast_Score=60, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR016032
- InterPro:   IPR011879
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 25703; Mature: 25572

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSADQPLVLVVEDEPAQREVLAYNLEAEGFAVSRAENGEEAMMLVDETAPDLIVLDWMMP
CCCCCCEEEEECCCCCCCEEEEEECCCCCEEEECCCCCCCEEEEEECCCCCEEEEEECCC
NLSGIEVCRRLKTRSETRAIPIIMLSARSEEVDRVRGLETGADDYVIKPYSIVELMARVR
CCCHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHCCCCCCCCEEECCHHHHHHHHHHH
SQLRRVRPAATGGQLEYEDIVLNAASHRVSRGENELKLGPTEFRLLSTFMEKPGRVFSRE
HHHHHHCCCCCCCCEEHHHHEEEHHHHHHHCCCCCEEECHHHHHHHHHHHHCCCCHHHHH
QLLDRVWGRDIYVDTRTVDVHIGRLRKALTQFGGSDPLRTVRGAGYALG
HHHHHHCCCEEEEEEEEEEEHHHHHHHHHHHCCCCCCHHHHHCCCCCCC
>Mature Secondary Structure 
SADQPLVLVVEDEPAQREVLAYNLEAEGFAVSRAENGEEAMMLVDETAPDLIVLDWMMP
CCCCCEEEEECCCCCCCEEEEEECCCCCEEEECCCCCCCEEEEEECCCCCEEEEEECCC
NLSGIEVCRRLKTRSETRAIPIIMLSARSEEVDRVRGLETGADDYVIKPYSIVELMARVR
CCCHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHCCCCCCCCEEECCHHHHHHHHHHH
SQLRRVRPAATGGQLEYEDIVLNAASHRVSRGENELKLGPTEFRLLSTFMEKPGRVFSRE
HHHHHHCCCCCCCCEEHHHHEEEHHHHHHHCCCCCEEECHHHHHHHHHHHHCCCCHHHHH
QLLDRVWGRDIYVDTRTVDVHIGRLRKALTQFGGSDPLRTVRGAGYALG
HHHHHHCCCEEEEEEEEEEEHHHHHHHHHHHCCCCCCHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]