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The map label for this gene is braE [H]

Identifier: 110679854

GI number: 110679854

Start: 2507099

End: 2508415

Strand: Reverse

Name: braE [H]

Synonym: RD1_2626

Alternate gene names: 110679854

Gene position: 2508415-2507099 (Counterclockwise)

Preceding gene: 110679855

Following gene: 110679852

Centisome position: 60.69

GC content: 59.91

Gene sequence:

>1317_bases
ATGAACCCGGTGCTGAAAAATACCATGCTGTTTGCGATCGTGGGCGCAATGATCCTCTATACCGGTTTCACGCAAAGCTG
GAACACGGCGCTTGTGATCGTCAACATGGGGCTGATTTCGGCGATCATGGCGCTGGGTGTGAACCTGCAATGGGGGTTTG
CGGGGCTGTTCAACGTCGGCATCATGGGTTTTGTGGCCCTTGGCGGTCTGGCGGCGGTTCTCATCGGTGCGCCGCCAACA
CCCGGCGCATGGAGCGCGGGGGGATGGAATATCCTGAGCGCATTCGTCTTTGCCCTTGTGATGGTGGTAGTGGTCGTCAG
CGTGCGCCGGCGCATGGCCGCTGGCTGGCCGCGTAAAATCGTCGTGCTGGCGCTGCTGATCGTTGGTTTCTTCATCTATC
GCGCCTTGTTCGATCCCGGTGTTGAGGCAATCGAGTCGATAAATCCCGCGGCGACGGGCAATATCGGCGGGCTGGGCATT
GATCGCGGGCTTTGGGTGACGCTGGCCTGGCCCGTTGGGGGGCTGTTTGCGGCGGCTGCGGCGTGGGTCATTGGCAAAAC
CGCGCTGGGATTACGTTCAGATTATCTGGCGATTGCCACGCTTGGCATTGCGGAGATCATCATTGCCGTTCTCAAGAACG
AAGACTGGCTCGCGCGCGGTGTTAAGAACGTCGTGGGCCTGCCGCGCCCCACGCCCAATGAAATCGATCTGCAAAACGAC
CCGGCCTTCGTGGAGCGCGCCGCAGGCTTTGGGGTGGATGCGGTCACTGCGTCGACACTTTATGTCAAGCTGATGTACGC
GGGGCTCTTTACGGTTGTGCTCGTGGTCCTGCTGGTGCTGGCGCAGCGCGCGCTGCACAGCCCATGGGGCCGGATGATGC
GCGCCATTCGCGACAATGAAGTGGCAGCCGAAGCCATGGGCAAGGACGTCACCGCGCGGCATTTGCAAATCTTTGTTCTC
GGGTCTGCCATTTGCGGCATTGCTGGGGCGATGATGACGACATTGGATGGCCAGTTGACGCCCGGCACCTATCAACCCTT
GCGCTTTACCTTTCTGGTCTGGGTGATGGTCATCGTAGGCGGGTCCGGCAACAACTTTGGCGCGATCCTCGGCGGTTTCC
TGATCTGGTTTCTATGGGTGCAGGTTGAACCGATGGGCCTTTGGTTGATGGAATTGATCACATCCGGCATGCCTGATGGG
TCCGCGCTCAAGGCGCATCTGATCGACTCTGCGGCGCATATGCGGCTGTTTACCATGGGCATGGTCCTGCTCTTGGTATT
GCGGTTCAGCCCGCGCGGGCTGATCCCGGAAAAGTAA

Upstream 100 bases:

>100_bases
TGCAGCTTTTGTCGACCGATTACAAACTGGCGGTGAGCTTTCTGATCCTGCTGATCGTGCTGTTGTTCAAGCCGACCGGA
TTGTTCAAAGGGAAATCCGT

Downstream 100 bases:

>100_bases
AAGCGGTAAACTAGCGCCCTTTGAACAGACTGCCGAGGATCCCGCGCACCATGCGCCGCCCCGTTGTCCCGCTCAACTCC
TTGAGGACGGCATTGGTGAG

Product: branched-chain amino acid ABC transporter, permease protein, putative

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 438; Mature: 438

Protein sequence:

>438_residues
MNPVLKNTMLFAIVGAMILYTGFTQSWNTALVIVNMGLISAIMALGVNLQWGFAGLFNVGIMGFVALGGLAAVLIGAPPT
PGAWSAGGWNILSAFVFALVMVVVVVSVRRRMAAGWPRKIVVLALLIVGFFIYRALFDPGVEAIESINPAATGNIGGLGI
DRGLWVTLAWPVGGLFAAAAAWVIGKTALGLRSDYLAIATLGIAEIIIAVLKNEDWLARGVKNVVGLPRPTPNEIDLQND
PAFVERAAGFGVDAVTASTLYVKLMYAGLFTVVLVVLLVLAQRALHSPWGRMMRAIRDNEVAAEAMGKDVTARHLQIFVL
GSAICGIAGAMMTTLDGQLTPGTYQPLRFTFLVWVMVIVGGSGNNFGAILGGFLIWFLWVQVEPMGLWLMELITSGMPDG
SALKAHLIDSAAHMRLFTMGMVLLLVLRFSPRGLIPEK

Sequences:

>Translated_438_residues
MNPVLKNTMLFAIVGAMILYTGFTQSWNTALVIVNMGLISAIMALGVNLQWGFAGLFNVGIMGFVALGGLAAVLIGAPPT
PGAWSAGGWNILSAFVFALVMVVVVVSVRRRMAAGWPRKIVVLALLIVGFFIYRALFDPGVEAIESINPAATGNIGGLGI
DRGLWVTLAWPVGGLFAAAAAWVIGKTALGLRSDYLAIATLGIAEIIIAVLKNEDWLARGVKNVVGLPRPTPNEIDLQND
PAFVERAAGFGVDAVTASTLYVKLMYAGLFTVVLVVLLVLAQRALHSPWGRMMRAIRDNEVAAEAMGKDVTARHLQIFVL
GSAICGIAGAMMTTLDGQLTPGTYQPLRFTFLVWVMVIVGGSGNNFGAILGGFLIWFLWVQVEPMGLWLMELITSGMPDG
SALKAHLIDSAAHMRLFTMGMVLLLVLRFSPRGLIPEK
>Mature_438_residues
MNPVLKNTMLFAIVGAMILYTGFTQSWNTALVIVNMGLISAIMALGVNLQWGFAGLFNVGIMGFVALGGLAAVLIGAPPT
PGAWSAGGWNILSAFVFALVMVVVVVSVRRRMAAGWPRKIVVLALLIVGFFIYRALFDPGVEAIESINPAATGNIGGLGI
DRGLWVTLAWPVGGLFAAAAAWVIGKTALGLRSDYLAIATLGIAEIIIAVLKNEDWLARGVKNVVGLPRPTPNEIDLQND
PAFVERAAGFGVDAVTASTLYVKLMYAGLFTVVLVVLLVLAQRALHSPWGRMMRAIRDNEVAAEAMGKDVTARHLQIFVL
GSAICGIAGAMMTTLDGQLTPGTYQPLRFTFLVWVMVIVGGSGNNFGAILGGFLIWFLWVQVEPMGLWLMELITSGMPDG
SALKAHLIDSAAHMRLFTMGMVLLLVLRFSPRGLIPEK

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG4177

COG function: function code E; ABC-type branched-chain amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=222, Percent_Identity=30.1801801801802, Blast_Score=82, Evalue=6e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 46718; Mature: 46718

Theoretical pI: Translated: 9.85; Mature: 9.85

Prosite motif: PS00211 ABC_TRANSPORTER_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPVLKNTMLFAIVGAMILYTGFTQSWNTALVIVNMGLISAIMALGVNLQWGFAGLFNVG
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHCCCCCCCHHHHHHHH
IMGFVALGGLAAVLIGAPPTPGAWSAGGWNILSAFVFALVMVVVVVSVRRRMAAGWPRKI
HHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
VVLALLIVGFFIYRALFDPGVEAIESINPAATGNIGGLGIDRGLWVTLAWPVGGLFAAAA
HHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHH
AWVIGKTALGLRSDYLAIATLGIAEIIIAVLKNEDWLARGVKNVVGLPRPTPNEIDLQND
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCC
PAFVERAAGFGVDAVTASTLYVKLMYAGLFTVVLVVLLVLAQRALHSPWGRMMRAIRDNE
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
VAAEAMGKDVTARHLQIFVLGSAICGIAGAMMTTLDGQLTPGTYQPLRFTFLVWVMVIVG
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHC
GSGNNFGAILGGFLIWFLWVQVEPMGLWLMELITSGMPDGSALKAHLIDSAAHMRLFTMG
CCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
MVLLLVLRFSPRGLIPEK
HHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MNPVLKNTMLFAIVGAMILYTGFTQSWNTALVIVNMGLISAIMALGVNLQWGFAGLFNVG
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHCCCCCCCHHHHHHHH
IMGFVALGGLAAVLIGAPPTPGAWSAGGWNILSAFVFALVMVVVVVSVRRRMAAGWPRKI
HHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
VVLALLIVGFFIYRALFDPGVEAIESINPAATGNIGGLGIDRGLWVTLAWPVGGLFAAAA
HHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHH
AWVIGKTALGLRSDYLAIATLGIAEIIIAVLKNEDWLARGVKNVVGLPRPTPNEIDLQND
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCC
PAFVERAAGFGVDAVTASTLYVKLMYAGLFTVVLVVLLVLAQRALHSPWGRMMRAIRDNE
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
VAAEAMGKDVTARHLQIFVLGSAICGIAGAMMTTLDGQLTPGTYQPLRFTFLVWVMVIVG
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHC
GSGNNFGAILGGFLIWFLWVQVEPMGLWLMELITSGMPDGSALKAHLIDSAAHMRLFTMG
CCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
MVLLLVLRFSPRGLIPEK
HHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]