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The map label for this gene is ligA

Identifier: 110679832

GI number: 110679832

Start: 2484611

End: 2486728

Strand: Direct

Name: ligA

Synonym: RD1_2602

Alternate gene names: 110679832

Gene position: 2484611-2486728 (Clockwise)

Preceding gene: 110679831

Following gene: 110679833

Centisome position: 60.11

GC content: 56.56

Gene sequence:

>2118_bases
ATGGATTTCGCCCCCCTTGACAGTCTGACCGAAAAAGCGGCGAAGAAAGAGCTGTCCAAGCTCGCAGATCTTCTGGATCA
GGCGAATACCGCGTATCACGCAAACGACGCACCGATTATATCTGACGCTGATTTTGACGCATACAAGCGTCGCAACCTTG
CGATTGAAGAACGCTTTCCCCACCTGAAACGCGCCGACAGCCCGACCGATCGCGTCGGCGCAGCGCCGTCAGCCGGTTTT
TCCAAGGTGGTTCATTCGATCGCCATGCTGTCACTGGCCAATGCCTTTGACGAGGCAGATGTTTCAGATTTCGTTCAGCG
CATTCGCAAACACCTTGGGTTGAGCGATATAGAGGCGTTGAGCTTCACATCAGAGCCAAAGATTGATGGCCTATCACTGT
CTCTCAGATATGAAAATGGTACTCTCGTTCAGGCCGCCACCCGTGGCGATGGGGCGATTGGTGAAAACGTCACCGAAAAC
GCGCGAACAATACCGGATATCCCCGAACAGATCTCAAATGCCCCCGATGTTCTCGAAGTTCGTGGTGAAGTCTATATGTC
TCACGCCGATTTCGAAGCGTTGAATGCCAGGCAAGCTTCGGCAGGCGCAAAACTGTTTGCAAATCCCCGGAATGCAGCGG
CCGGGTCCTTGCGCCAGCTTGACGCGCGTATCACCCAGAGCAGACCGCTCAGGTTTTTTGCATATGCCTGGGGAACACTC
TCCGAGCCTTTGGGGAAGACGCAAATGCAGTCGATCGAACGCCTGCAAAGCTTTGGGTTTCAAACGAATGACTTGACCGA
AAAATGCAATGGACCAGAAGAGTTGCTGGCGCAATACCGCCGCATCGAAGAGCGCCGCGCCTCCTTGGATTATGACATTG
ATGGGGTCGTCTACAAAGTGGATGATCTGGACTTGCAACGTCGGCTGGGCTTTCGCTCCACCACGCCCAGATGGGCCATC
GCACATAAATTTCCTGCTGAGCTTGCCTGGACCGAGTTGCAGGGTATCGACATTCAGGTCGGTCGCACCGGTGCGTTATC
CCCGGTCGCGCGCTTGAAACCTGTCACTGTCGGCGGTGTCGTCGTGTCAAATGCCACGCTGCACAACGAAGACTACATCG
CAGGGCGCGACAATAAGGGCGATGTTATCCGCGGTGGCAAGGATATCCGCGTGGGTGATTTTGTGCAGGTGTACCGTGCG
GGGGACGTCATTCCGAAAGTGGCTGACGTTGACCTTAAAAAGCGACCTGCGGATGCAATCCCATATGTCTTTCCGGCAAC
CTGTCCGGAATGTGGCAGTGACGCGCTGCGCGAGGCAGGCGATGCGGTGCGCAGATGCACCGGAGGCTTGATTTGCCCGG
CGCAGGCGGTGGAACGGCTCAAGCATTTCGTATCACGCGCGGCATTCGACATAGACGGTCTGGGTGCCAAGCAGATCGAG
CAGTTTTACAAAGATGGTTGGATATCGGAACCGGCTGACATCTTCACGCTGCGCGACCGATTCGGATCCGGCATTCAGCA
ATTGAAAAACCGTGATGGCTGGGGGGAGAAATCGGCAAACAACCTCTTCGATGCCATTGATGACAAGCGACAGATTCCTT
TGGCGCGGCTCATTTTCGCCCTTGGGATTCGCCATGTGGGCGAAGCGGCGTCCAATCTGATCGCGCAACACTATTCTACC
TTTGATGCCTTCGAGAAATCGATGCTGGCCGCGCAAGACAAGGACGGCGAGGCATGGGATGACCTGCTCAGCATCGACGG
TGTCGGGACGGTCATGGCGCAATCGGTCATTCATGCGATGGGCCAAGCGGCCGAACGTGCATCCATCGACCGCCTCGTGG
CCCAATTGGACGTGCAGCCGTCAGAAGCTGTCGTGACGGACGGCAGCCCGGTGGCGGGAAAGACCGTGGTGTTTACGGGC
ACGCTGAGCAAAATGACCCGCGCGGAAGCCAAATCACGGGCCGAAAGCCTTGGGGCAAGGGTTGCCGGTTCCGTGTCAGC
CAAAACCGACTTCCTTGTTGCAGGACCCGGCGCAGGTTCAAAGGCCAAGAAAGCCGCCGAACTCGGTGTTCAAACCCTCG
ACGAAGACGGCTGGCTGGCACTTATCGAGGGCTTATGA

Upstream 100 bases:

>100_bases
TCGCGTCTGTTTTGTCAGATCAGTCATTTCGCAAAACAACTGGACCTCTGTGCGACCTATGCCTAACTACGTGTGAGCAA
ATGGCAATCGAACAGGTCCC

Downstream 100 bases:

>100_bases
GCGGTCGTCCGGAAGCTCTGTTTGGCCTGTTTTCATCTCTTGAAACCCTTGACGGAGTTGGACCCAAAACAGCTGCGAAT
TTTGCCGGTCTGGGCGTTCA

Product: DNA ligase, NAD-dependent

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+]

Number of amino acids: Translated: 705; Mature: 705

Protein sequence:

>705_residues
MDFAPLDSLTEKAAKKELSKLADLLDQANTAYHANDAPIISDADFDAYKRRNLAIEERFPHLKRADSPTDRVGAAPSAGF
SKVVHSIAMLSLANAFDEADVSDFVQRIRKHLGLSDIEALSFTSEPKIDGLSLSLRYENGTLVQAATRGDGAIGENVTEN
ARTIPDIPEQISNAPDVLEVRGEVYMSHADFEALNARQASAGAKLFANPRNAAAGSLRQLDARITQSRPLRFFAYAWGTL
SEPLGKTQMQSIERLQSFGFQTNDLTEKCNGPEELLAQYRRIEERRASLDYDIDGVVYKVDDLDLQRRLGFRSTTPRWAI
AHKFPAELAWTELQGIDIQVGRTGALSPVARLKPVTVGGVVVSNATLHNEDYIAGRDNKGDVIRGGKDIRVGDFVQVYRA
GDVIPKVADVDLKKRPADAIPYVFPATCPECGSDALREAGDAVRRCTGGLICPAQAVERLKHFVSRAAFDIDGLGAKQIE
QFYKDGWISEPADIFTLRDRFGSGIQQLKNRDGWGEKSANNLFDAIDDKRQIPLARLIFALGIRHVGEAASNLIAQHYST
FDAFEKSMLAAQDKDGEAWDDLLSIDGVGTVMAQSVIHAMGQAAERASIDRLVAQLDVQPSEAVVTDGSPVAGKTVVFTG
TLSKMTRAEAKSRAESLGARVAGSVSAKTDFLVAGPGAGSKAKKAAELGVQTLDEDGWLALIEGL

Sequences:

>Translated_705_residues
MDFAPLDSLTEKAAKKELSKLADLLDQANTAYHANDAPIISDADFDAYKRRNLAIEERFPHLKRADSPTDRVGAAPSAGF
SKVVHSIAMLSLANAFDEADVSDFVQRIRKHLGLSDIEALSFTSEPKIDGLSLSLRYENGTLVQAATRGDGAIGENVTEN
ARTIPDIPEQISNAPDVLEVRGEVYMSHADFEALNARQASAGAKLFANPRNAAAGSLRQLDARITQSRPLRFFAYAWGTL
SEPLGKTQMQSIERLQSFGFQTNDLTEKCNGPEELLAQYRRIEERRASLDYDIDGVVYKVDDLDLQRRLGFRSTTPRWAI
AHKFPAELAWTELQGIDIQVGRTGALSPVARLKPVTVGGVVVSNATLHNEDYIAGRDNKGDVIRGGKDIRVGDFVQVYRA
GDVIPKVADVDLKKRPADAIPYVFPATCPECGSDALREAGDAVRRCTGGLICPAQAVERLKHFVSRAAFDIDGLGAKQIE
QFYKDGWISEPADIFTLRDRFGSGIQQLKNRDGWGEKSANNLFDAIDDKRQIPLARLIFALGIRHVGEAASNLIAQHYST
FDAFEKSMLAAQDKDGEAWDDLLSIDGVGTVMAQSVIHAMGQAAERASIDRLVAQLDVQPSEAVVTDGSPVAGKTVVFTG
TLSKMTRAEAKSRAESLGARVAGSVSAKTDFLVAGPGAGSKAKKAAELGVQTLDEDGWLALIEGL
>Mature_705_residues
MDFAPLDSLTEKAAKKELSKLADLLDQANTAYHANDAPIISDADFDAYKRRNLAIEERFPHLKRADSPTDRVGAAPSAGF
SKVVHSIAMLSLANAFDEADVSDFVQRIRKHLGLSDIEALSFTSEPKIDGLSLSLRYENGTLVQAATRGDGAIGENVTEN
ARTIPDIPEQISNAPDVLEVRGEVYMSHADFEALNARQASAGAKLFANPRNAAAGSLRQLDARITQSRPLRFFAYAWGTL
SEPLGKTQMQSIERLQSFGFQTNDLTEKCNGPEELLAQYRRIEERRASLDYDIDGVVYKVDDLDLQRRLGFRSTTPRWAI
AHKFPAELAWTELQGIDIQVGRTGALSPVARLKPVTVGGVVVSNATLHNEDYIAGRDNKGDVIRGGKDIRVGDFVQVYRA
GDVIPKVADVDLKKRPADAIPYVFPATCPECGSDALREAGDAVRRCTGGLICPAQAVERLKHFVSRAAFDIDGLGAKQIE
QFYKDGWISEPADIFTLRDRFGSGIQQLKNRDGWGEKSANNLFDAIDDKRQIPLARLIFALGIRHVGEAASNLIAQHYST
FDAFEKSMLAAQDKDGEAWDDLLSIDGVGTVMAQSVIHAMGQAAERASIDRLVAQLDVQPSEAVVTDGSPVAGKTVVFTG
TLSKMTRAEAKSRAESLGARVAGSVSAKTDFLVAGPGAGSKAKKAAELGVQTLDEDGWLALIEGL

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain

Homologues:

Organism=Escherichia coli, GI1788750, Length=697, Percent_Identity=45.480631276901, Blast_Score=572, Evalue=1e-164,
Organism=Escherichia coli, GI87082305, Length=546, Percent_Identity=24.5421245421245, Blast_Score=96, Evalue=6e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLJ_ROSDO (Q166E0)

Other databases:

- EMBL:   CP000362
- RefSeq:   YP_682839.1
- ProteinModelPortal:   Q166E0
- STRING:   Q166E0
- GeneID:   4194963
- GenomeReviews:   CP000362_GR
- KEGG:   rde:RD1_2602
- NMPDR:   fig|375451.6.peg.2418
- eggNOG:   COG0272
- HOGENOM:   HBG620317
- OMA:   IKHFASR
- PhylomeDB:   Q166E0
- ProtClustDB:   CLSK933607
- BioCyc:   RDEN375451:RD1_2602-MONOMER
- GO:   GO:0005622
- HAMAP:   MF_01588
- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149
- Gene3D:   G3DSA:2.40.50.140
- PIRSF:   PIRSF001604
- SMART:   SM00292
- SMART:   SM00278
- SMART:   SM00532
- TIGRFAMs:   TIGR00575

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD; SSF52113 BRCT; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =6.5.1.2

Molecular weight: Translated: 76412; Mature: 76412

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: PS50172 BRCT; PS01055 DNA_LIGASE_N1; PS01056 DNA_LIGASE_N2

Important sites: ACT_SITE 127-127 BINDING 125-125 BINDING 148-148 BINDING 183-183 BINDING 299-299 BINDING 323-323

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFAPLDSLTEKAAKKELSKLADLLDQANTAYHANDAPIISDADFDAYKRRNLAIEERFP
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCEECCCCCHHHHHCCCCHHHHCC
HLKRADSPTDRVGAAPSAGFSKVVHSIAMLSLANAFDEADVSDFVQRIRKHLGLSDIEAL
HHHCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHH
SFTSEPKIDGLSLSLRYENGTLVQAATRGDGAIGENVTENARTIPDIPEQISNAPDVLEV
CCCCCCCCCCEEEEEEECCCCEEEEECCCCCCCCCCCCCCCCCCCCCHHHHCCCCCHHHC
RGEVYMSHADFEALNARQASAGAKLFANPRNAAAGSLRQLDARITQSRPLRFFAYAWGTL
CCHHEEECCCHHHHHHHHHHCCCCEECCCCCHHHHHHHHHHHHHHCCCCCEEEEEEHHHH
SEPLGKTQMQSIERLQSFGFQTNDLTEKCNGPEELLAQYRRIEERRASLDYDIDGVVYKV
HCCCCHHHHHHHHHHHHCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEE
DDLDLQRRLGFRSTTPRWAIAHKFPAELAWTELQGIDIQVGRTGALSPVARLKPVTVGGV
CCCCHHHHCCCCCCCCCEEEEECCCCHHHHHHHCCCEEEECCCCCCCHHHHCCCEEECCE
VVSNATLHNEDYIAGRDNKGDVIRGGKDIRVGDFVQVYRAGDVIPKVADVDLKKRPADAI
EEECCEECCCCCCCCCCCCCCEEECCCCCCHHHHHHHHHHCCCCCCHHCCCCCCCCCCCC
PYVFPATCPECGSDALREAGDAVRRCTGGLICPAQAVERLKHFVSRAAFDIDGLGAKQIE
CEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHH
QFYKDGWISEPADIFTLRDRFGSGIQQLKNRDGWGEKSANNLFDAIDDKRQIPLARLIFA
HHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHH
LGIRHVGEAASNLIAQHYSTFDAFEKSMLAAQDKDGEAWDDLLSIDGVGTVMAQSVIHAM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHH
GQAAERASIDRLVAQLDVQPSEAVVTDGSPVAGKTVVFTGTLSKMTRAEAKSRAESLGAR
HHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHH
VAGSVSAKTDFLVAGPGAGSKAKKAAELGVQTLDEDGWLALIEGL
HHCCCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCEEEECCC
>Mature Secondary Structure
MDFAPLDSLTEKAAKKELSKLADLLDQANTAYHANDAPIISDADFDAYKRRNLAIEERFP
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCEECCCCCHHHHHCCCCHHHHCC
HLKRADSPTDRVGAAPSAGFSKVVHSIAMLSLANAFDEADVSDFVQRIRKHLGLSDIEAL
HHHCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHH
SFTSEPKIDGLSLSLRYENGTLVQAATRGDGAIGENVTENARTIPDIPEQISNAPDVLEV
CCCCCCCCCCEEEEEEECCCCEEEEECCCCCCCCCCCCCCCCCCCCCHHHHCCCCCHHHC
RGEVYMSHADFEALNARQASAGAKLFANPRNAAAGSLRQLDARITQSRPLRFFAYAWGTL
CCHHEEECCCHHHHHHHHHHCCCCEECCCCCHHHHHHHHHHHHHHCCCCCEEEEEEHHHH
SEPLGKTQMQSIERLQSFGFQTNDLTEKCNGPEELLAQYRRIEERRASLDYDIDGVVYKV
HCCCCHHHHHHHHHHHHCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEE
DDLDLQRRLGFRSTTPRWAIAHKFPAELAWTELQGIDIQVGRTGALSPVARLKPVTVGGV
CCCCHHHHCCCCCCCCCEEEEECCCCHHHHHHHCCCEEEECCCCCCCHHHHCCCEEECCE
VVSNATLHNEDYIAGRDNKGDVIRGGKDIRVGDFVQVYRAGDVIPKVADVDLKKRPADAI
EEECCEECCCCCCCCCCCCCCEEECCCCCCHHHHHHHHHHCCCCCCHHCCCCCCCCCCCC
PYVFPATCPECGSDALREAGDAVRRCTGGLICPAQAVERLKHFVSRAAFDIDGLGAKQIE
CEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHH
QFYKDGWISEPADIFTLRDRFGSGIQQLKNRDGWGEKSANNLFDAIDDKRQIPLARLIFA
HHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHH
LGIRHVGEAASNLIAQHYSTFDAFEKSMLAAQDKDGEAWDDLLSIDGVGTVMAQSVIHAM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHH
GQAAERASIDRLVAQLDVQPSEAVVTDGSPVAGKTVVFTGTLSKMTRAEAKSRAESLGAR
HHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHH
VAGSVSAKTDFLVAGPGAGSKAKKAAELGVQTLDEDGWLALIEGL
HHCCCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA