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The map label for this gene is purB [H]

Identifier: 110679795

GI number: 110679795

Start: 2447942

End: 2449246

Strand: Reverse

Name: purB [H]

Synonym: RD1_2559

Alternate gene names: 110679795

Gene position: 2449246-2447942 (Counterclockwise)

Preceding gene: 110679799

Following gene: 110679794

Centisome position: 59.26

GC content: 58.08

Gene sequence:

>1305_bases
ATGATCCCCCGTTACTCCCGCCCCGACATGGTCGCCATCTGGAGCCCGGAGACGAAGTTCCGCATCTGGTATGAAATCGA
AGCCCATGCCTGCGAAGCCATGGCCAACCTTGGTGTCATCCCGCGCGAAAACGCCGACGCGGTCTGGAAAGCCAAGGACG
TGGAGTTCGATGTCGCCCGGATTGACGAGATCGAAGCGGTCACGAAACATGACGTCATCGCCTTTCTGACCCATCTGGCT
GAACATGTTGGCAGCGAAGAGGCGCGGTTTGTGCATCAGGGCATGACCTCTTCGGATGTGCTGGATACATGCTTCAACGT
GCAGCTTGTGCGCGCCTCCGACATTCTGATTGCCGACATCAAGGCGCTGCTCGCCGCACTCAAACGCCGCGCGATTGAGC
ATAAGGACACCGTCAGAATCGGGCGCAGCCACGGCATTCACGCGGAACCCACGACCATGGGGCTGACCTTTGCGCGGTTT
TATGCAGAGATGGACCGCAACCTGAACCGGATGGAAAAGGCGCGTTTTGAAATCGCGACCGGTGCCATTTCCGGCGCTGT
CGGCACCTTTGCAAACATCGACCCGCGTGTTGAGGAACATGTCTGCGCGCAGCTTGGTCTTGAGCCAGAGCCGATCAGCA
CACAGGTGATCCCGCGCGACCGACATGCCGCGTTTTTCGCAACTCTCGGGGTGATCGGGTCCTCCATCGAGAACATCGCC
ACCGAAATCCGCCACATGCAGCGCACCGAAGTGCTGGAAGCGGAGGAGTTCTTTTCCGCGGGGCAGAAAGGTTCTTCCGC
CATGCCGCACAAGCGCAACCCGGTGCTGACCGAAAACCTGACCGGTCTTGCACGGCTGGTACGGATGGCCGTGGTGCCTG
CGATGGAAAATGTTGCCCTGTGGCACGAGCGCGACATCTCGCATTCTTCGGTCGAGCGCAACATTGGCCCTGACACGACG
ATCACGTTGGATTTTGCGCTGGCCCGCCTGACATCGGTGGTGGACAAGTTGGTGATCTATCCGGATAACATGCTGGCCAA
CATGAACAAATTCCGGGGTCTGGTGATGAGTCAGCGTGTTCTGCTGGCCCTGACGCAGGCCGGTGTGAGCCGCGAAGACA
GCTATAAAATGGTGCAGCGCAACGCGATGAAAGTGTGGGAAGAAGGCAAGGATTTCAAAACCGAACTGCTGGGGGATGCG
GATGTGCTTGCCGCCCTGAGCGCAGAGGAAATCGAAGAGAAATTCGACCTCGGATATCACACCAAACATGTGGATACGAT
TTTCGCGCGTGTGTTCAAGGATTAG

Upstream 100 bases:

>100_bases
CCAAAGACCCCGCATTTTGCCGCTCCTGCCTTGTGATACGCCGCGCGCCAGTCTATGAGCCGCCCAAACCCGGACGCAAT
CGCAAATAAGGACGCATTCC

Downstream 100 bases:

>100_bases
GCACCGGCAACTCGGTGGACACGAGCCGCGTTATTTTTGTTGACCCGTGTCGCGCGGCTGCTACTTTTAACGATGTACTC
AAACAGGAGAACCGCAATGA

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase; Glutamyl--tRNA ligase regulatory factor [H]

Number of amino acids: Translated: 434; Mature: 434

Protein sequence:

>434_residues
MIPRYSRPDMVAIWSPETKFRIWYEIEAHACEAMANLGVIPRENADAVWKAKDVEFDVARIDEIEAVTKHDVIAFLTHLA
EHVGSEEARFVHQGMTSSDVLDTCFNVQLVRASDILIADIKALLAALKRRAIEHKDTVRIGRSHGIHAEPTTMGLTFARF
YAEMDRNLNRMEKARFEIATGAISGAVGTFANIDPRVEEHVCAQLGLEPEPISTQVIPRDRHAAFFATLGVIGSSIENIA
TEIRHMQRTEVLEAEEFFSAGQKGSSAMPHKRNPVLTENLTGLARLVRMAVVPAMENVALWHERDISHSSVERNIGPDTT
ITLDFALARLTSVVDKLVIYPDNMLANMNKFRGLVMSQRVLLALTQAGVSREDSYKMVQRNAMKVWEEGKDFKTELLGDA
DVLAALSAEEIEEKFDLGYHTKHVDTIFARVFKD

Sequences:

>Translated_434_residues
MIPRYSRPDMVAIWSPETKFRIWYEIEAHACEAMANLGVIPRENADAVWKAKDVEFDVARIDEIEAVTKHDVIAFLTHLA
EHVGSEEARFVHQGMTSSDVLDTCFNVQLVRASDILIADIKALLAALKRRAIEHKDTVRIGRSHGIHAEPTTMGLTFARF
YAEMDRNLNRMEKARFEIATGAISGAVGTFANIDPRVEEHVCAQLGLEPEPISTQVIPRDRHAAFFATLGVIGSSIENIA
TEIRHMQRTEVLEAEEFFSAGQKGSSAMPHKRNPVLTENLTGLARLVRMAVVPAMENVALWHERDISHSSVERNIGPDTT
ITLDFALARLTSVVDKLVIYPDNMLANMNKFRGLVMSQRVLLALTQAGVSREDSYKMVQRNAMKVWEEGKDFKTELLGDA
DVLAALSAEEIEEKFDLGYHTKHVDTIFARVFKD
>Mature_434_residues
MIPRYSRPDMVAIWSPETKFRIWYEIEAHACEAMANLGVIPRENADAVWKAKDVEFDVARIDEIEAVTKHDVIAFLTHLA
EHVGSEEARFVHQGMTSSDVLDTCFNVQLVRASDILIADIKALLAALKRRAIEHKDTVRIGRSHGIHAEPTTMGLTFARF
YAEMDRNLNRMEKARFEIATGAISGAVGTFANIDPRVEEHVCAQLGLEPEPISTQVIPRDRHAAFFATLGVIGSSIENIA
TEIRHMQRTEVLEAEEFFSAGQKGSSAMPHKRNPVLTENLTGLARLVRMAVVPAMENVALWHERDISHSSVERNIGPDTT
ITLDFALARLTSVVDKLVIYPDNMLANMNKFRGLVMSQRVLLALTQAGVSREDSYKMVQRNAMKVWEEGKDFKTELLGDA
DVLAALSAEEIEEKFDLGYHTKHVDTIFARVFKD

Specific function: Influences the affinity of glutamyl--tRNA ligase for its substrates and increases its thermostability [H]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557269, Length=404, Percent_Identity=27.4752475247525, Blast_Score=134, Evalue=1e-31,
Organism=Homo sapiens, GI183227688, Length=381, Percent_Identity=27.2965879265092, Blast_Score=127, Evalue=2e-29,
Organism=Escherichia coli, GI1787376, Length=363, Percent_Identity=28.099173553719, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1787896, Length=208, Percent_Identity=29.3269230769231, Blast_Score=62, Evalue=9e-11,
Organism=Caenorhabditis elegans, GI17508577, Length=403, Percent_Identity=26.7990074441687, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI32564234, Length=358, Percent_Identity=27.6536312849162, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17553882, Length=267, Percent_Identity=26.5917602996255, Blast_Score=69, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI32565146, Length=262, Percent_Identity=26.7175572519084, Blast_Score=67, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6323391, Length=405, Percent_Identity=27.1604938271605, Blast_Score=127, Evalue=4e-30,
Organism=Drosophila melanogaster, GI24647570, Length=413, Percent_Identity=26.3922518159806, Blast_Score=105, Evalue=5e-23,
Organism=Drosophila melanogaster, GI78710009, Length=210, Percent_Identity=27.1428571428571, Blast_Score=68, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 48621; Mature: 48621

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPRYSRPDMVAIWSPETKFRIWYEIEAHACEAMANLGVIPRENADAVWKAKDVEFDVAR
CCCCCCCCCEEEEECCCCCEEEEEEEHHHHHHHHHHCCCCCCCCCCCEEECCCCEEHHHH
IDEIEAVTKHDVIAFLTHLAEHVGSEEARFVHQGMTSSDVLDTCFNVQLVRASDILIADI
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHCEEEEECCCHHHHHH
KALLAALKRRAIEHKDTVRIGRSHGIHAEPTTMGLTFARFYAEMDRNLNRMEKARFEIAT
HHHHHHHHHHHHCCHHHHEECCCCCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHH
GAISGAVGTFANIDPRVEEHVCAQLGLEPEPISTQVIPRDRHAAFFATLGVIGSSIENIA
HHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
TEIRHMQRTEVLEAEEFFSAGQKGSSAMPHKRNPVLTENLTGLARLVRMAVVPAMENVAL
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
WHERDISHSSVERNIGPDTTITLDFALARLTSVVDKLVIYPDNMLANMNKFRGLVMSQRV
HHHCCCCHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHHHH
LLALTQAGVSREDSYKMVQRNAMKVWEEGKDFKTELLGDADVLAALSAEEIEEKFDLGYH
HHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCHHHHHCCCHHHHHHHHHHHHHHHHCCCCC
TKHVDTIFARVFKD
HHHHHHHHHHHHCC
>Mature Secondary Structure
MIPRYSRPDMVAIWSPETKFRIWYEIEAHACEAMANLGVIPRENADAVWKAKDVEFDVAR
CCCCCCCCCEEEEECCCCCEEEEEEEHHHHHHHHHHCCCCCCCCCCCEEECCCCEEHHHH
IDEIEAVTKHDVIAFLTHLAEHVGSEEARFVHQGMTSSDVLDTCFNVQLVRASDILIADI
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHCEEEEECCCHHHHHH
KALLAALKRRAIEHKDTVRIGRSHGIHAEPTTMGLTFARFYAEMDRNLNRMEKARFEIAT
HHHHHHHHHHHHCCHHHHEECCCCCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHH
GAISGAVGTFANIDPRVEEHVCAQLGLEPEPISTQVIPRDRHAAFFATLGVIGSSIENIA
HHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
TEIRHMQRTEVLEAEEFFSAGQKGSSAMPHKRNPVLTENLTGLARLVRMAVVPAMENVAL
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
WHERDISHSSVERNIGPDTTITLDFALARLTSVVDKLVIYPDNMLANMNKFRGLVMSQRV
HHHCCCCHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHHHH
LLALTQAGVSREDSYKMVQRNAMKVWEEGKDFKTELLGDADVLAALSAEEIEEKFDLGYH
HHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCHHHHHCCCHHHHHHHHHHHHHHHHCCCCC
TKHVDTIFARVFKD
HHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3036807; 9384377; 1608947; 10926519 [H]