Click here to switch to the map view.

The map label for this gene is fixK [H]

Identifier: 110679696

GI number: 110679696

Start: 2339937

End: 2340665

Strand: Direct

Name: fixK [H]

Synonym: RD1_2451

Alternate gene names: 110679696

Gene position: 2339937-2340665 (Clockwise)

Preceding gene: 110679693

Following gene: 110679697

Centisome position: 56.61

GC content: 55.97

Gene sequence:

>729_bases
ATGGTTACGGACTGCAAGAACTGTCCGTTACGACAGCATGACCTTTTTCAGCCAATGTCAAAGGAAGAGGTTGAAAGAAC
GCAGAAATTCAAGTCCGGCGAACTGTCTGTCGATGCCGGCACACCCATTTTGATGGAAGGCTCGAATGCGCCGCAGATAT
ACACGGCACTGCGTGGCATGGGGCTGCGGTACAAGATCATGCCGGATGGGCGGCGGCAGGTGGTGAACTTTATTTTTCCC
GGCGACTTTATTGGTCTGCAGGCGGGTCTGATCGGGGAAATGGGGCATTCCGTTGAAGCGTCGACGCATATGAAACTATG
TGTTTTTGACCGCAAAGACTTCTTTGACTTTTTTCGAAACTCCACGTCGCGTGCCTTCGATGTGACGTGGCTGGCCGCAT
CAGAAGAGCACTTTCTGGGCGAAGCCCTTGCAACCGTGGGACAAAGGACGGCACTGGCGGGGGTGGCCTGGGCCTTGACG
CAGCTTTTCATGCGCGCCGATGCGCGAAACATGGTGCAGAATGACACGATGCCTTTGCCGTTTCGCCAACAGGATCTGGC
GGACGCGCTGGGGCTTTCATTGGTACATACGAACAAGACGCTCGCCCGCCTGCGCGAGCGCCAGCTTGCCAATTGGTCCG
ACCGCAGGCTTGTGATCCACGATCTCGAAGAACTTGCGAAAGTCGCCGAGATGGAACTGAACGATCTCAGGCCGAGGCCG
CTGATCTGA

Upstream 100 bases:

>100_bases
AAAAGTTCCGCATCGTTCTTCTCACAAATGCAAAACGGCTTTTTGGGAACAAACGCCCCGAATTGGCGTTGAGACCTCAC
AGGAAGTGGAGGAACACCCT

Downstream 100 bases:

>100_bases
TGCGTTGCATTTGCGAGTCGCGCACCAAAAGGAAGCGCGATGTTTGAGGTCAGCCAATCCGAATTCATGGTGCGCCTTGT
GGTTGCAGCACTGTGCGGTC

Product: nitrogen fixation regulatory protein fixK, putative

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 242; Mature: 242

Protein sequence:

>242_residues
MVTDCKNCPLRQHDLFQPMSKEEVERTQKFKSGELSVDAGTPILMEGSNAPQIYTALRGMGLRYKIMPDGRRQVVNFIFP
GDFIGLQAGLIGEMGHSVEASTHMKLCVFDRKDFFDFFRNSTSRAFDVTWLAASEEHFLGEALATVGQRTALAGVAWALT
QLFMRADARNMVQNDTMPLPFRQQDLADALGLSLVHTNKTLARLRERQLANWSDRRLVIHDLEELAKVAEMELNDLRPRP
LI

Sequences:

>Translated_242_residues
MVTDCKNCPLRQHDLFQPMSKEEVERTQKFKSGELSVDAGTPILMEGSNAPQIYTALRGMGLRYKIMPDGRRQVVNFIFP
GDFIGLQAGLIGEMGHSVEASTHMKLCVFDRKDFFDFFRNSTSRAFDVTWLAASEEHFLGEALATVGQRTALAGVAWALT
QLFMRADARNMVQNDTMPLPFRQQDLADALGLSLVHTNKTLARLRERQLANWSDRRLVIHDLEELAKVAEMELNDLRPRP
LI
>Mature_242_residues
MVTDCKNCPLRQHDLFQPMSKEEVERTQKFKSGELSVDAGTPILMEGSNAPQIYTALRGMGLRYKIMPDGRRQVVNFIFP
GDFIGLQAGLIGEMGHSVEASTHMKLCVFDRKDFFDFFRNSTSRAFDVTWLAASEEHFLGEALATVGQRTALAGVAWALT
QLFMRADARNMVQNDTMPLPFRQQDLADALGLSLVHTNKTLARLRERQLANWSDRRLVIHDLEELAKVAEMELNDLRPRP
LI

Specific function: B.japonicum has two fixLJ-dependent fixK homologs that are activators of the transcription of a group of genes involved in anaerobic processes such as denitrification and possibly nitrogen fixation. FixK may bind DNA at the FNR consensus binding site [H]

COG id: COG0664

COG function: function code T; cAMP-binding proteins - catabolite gene activator and regulatory subunit of cAMP-dependent protein kinases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH crp-type DNA-binding domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018490
- InterPro:   IPR000595
- InterPro:   IPR001808
- InterPro:   IPR012318
- InterPro:   IPR014710
- InterPro:   IPR018335
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00027 cNMP_binding; PF00325 Crp [H]

EC number: NA

Molecular weight: Translated: 27397; Mature: 27397

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS50042 CNMP_BINDING_3 ; PS51063 HTH_CRP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVTDCKNCPLRQHDLFQPMSKEEVERTQKFKSGELSVDAGTPILMEGSNAPQIYTALRGM
CCCCCCCCCCCHHHHHCCCCHHHHHHHHHHCCCCEEECCCCEEEEECCCCCHHHHHHHCC
GLRYKIMPDGRRQVVNFIFPGDFIGLQAGLIGEMGHSVEASTHMKLCVFDRKDFFDFFRN
CCEEEECCCCHHHHEEEECCCCHHHHHHHHHHHCCCCCCCCCCEEEEEECCHHHHHHHHC
STSRAFDVTWLAASEEHFLGEALATVGQRTALAGVAWALTQLFMRADARNMVQNDTMPLP
CCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
FRQQDLADALGLSLVHTNKTLARLRERQLANWSDRRLVIHDLEELAKVAEMELNDLRPRP
CCHHHHHHHHCCCCEECHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHCCCCC
LI
CC
>Mature Secondary Structure
MVTDCKNCPLRQHDLFQPMSKEEVERTQKFKSGELSVDAGTPILMEGSNAPQIYTALRGM
CCCCCCCCCCCHHHHHCCCCHHHHHHHHHHCCCCEEECCCCEEEEECCCCCHHHHHHHCC
GLRYKIMPDGRRQVVNFIFPGDFIGLQAGLIGEMGHSVEASTHMKLCVFDRKDFFDFFRN
CCEEEECCCCHHHHEEEECCCCHHHHHHHHHHHCCCCCCCCCCEEEEEECCHHHHHHHHC
STSRAFDVTWLAASEEHFLGEALATVGQRTALAGVAWALTQLFMRADARNMVQNDTMPLP
CCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
FRQQDLADALGLSLVHTNKTLARLRERQLANWSDRRLVIHDLEELAKVAEMELNDLRPRP
CCHHHHHHHHCCCCEECHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHCCCCC
LI
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1551834; 12597275 [H]