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The map label for this gene is narI [H]

Identifier: 110679690

GI number: 110679690

Start: 2331892

End: 2332686

Strand: Direct

Name: narI [H]

Synonym: RD1_2445

Alternate gene names: 110679690

Gene position: 2331892-2332686 (Clockwise)

Preceding gene: 110679689

Following gene: 110679691

Centisome position: 56.42

GC content: 56.86

Gene sequence:

>795_bases
ATGAACCAGTTTCTTTTCGGAACCTTTCCTTACATCGCACTGGCGATTTGCATCATCGGCTCGATCGCACGATACGAGCG
TGATCCATTCACATGGAAGTCTTCTTCATCGCAGATGCTGCGGCGCAGGCAACTCATCATTGGCTCGGTTCTGTTCCATG
TCGGTGTCCTGGTGATCTTCTTCGGTCACCTGGTTGGATTGCTGACACCGATCTGGATTTTCGATGCCATCGGCATCAGC
CACGGTGCAAAGCAACTCCTGGCCGTGGTGGCTGGCGGTATCGCGGGCGTTGCGGCGCTGATTGGGGGCATCCTGCTTTT
CCATCGCCGGTGGACTGATCCGCGGATCGCGAAGAATTCCAGCTTCTGGGACAACGCGATCCTGCTGATGTTGATTGCTC
AGCTGGTACTGGGGTTGGGCACCATTTTCGTCTCCCTCGGTCACTTGGACGGGCACGAGATGGTAAAGTTCATGTCCTGG
GCGCAGGGTATCTTCACCTTTGATGGTGCGGCCGCCGGATACGTCGCGGATGTGGCGATCGTCTTCAAGCTGCACTTGAT
CCTTGGCCTGCTGATCATCGCGGTCTTTCCGTTCACACGGCTTGTGCACATTCTGTCAGGATTAGCGGCACCGTTCCGGT
ATCTATTTGGACGCACCGGCTACCAGGTCGTTCGTTCACGTCGCAAGGTTGCCTTGCCGAAAACTGTCGGTGCCGGTTCT
CAAGGCCGCAAGAGCCCGATGCCAGAACGCAAGGGTGCAGCAGCACCTGACGCAATGCCAACCCCTGCGGAGTAA

Upstream 100 bases:

>100_bases
ATCCCAACGCAGGATGCCCACAAGTGCGCGACATGCTGTCTCGCATGGATCAACCCAACACCCCCACACCGCAACACGCG
GCCGAATGACGGAGGATGTC

Downstream 100 bases:

>100_bases
TCGTCATGAATGCGCTTTTTCCTGATCTTGTCGTAAATGGTGAAACGGTCCCGTCAGCACGGATTGCGGCAGAGACCCAA
AACCACGATGGCCCAAAGGG

Product: nitrate reductase, gamma subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MNQFLFGTFPYIALAICIIGSIARYERDPFTWKSSSSQMLRRRQLIIGSVLFHVGVLVIFFGHLVGLLTPIWIFDAIGIS
HGAKQLLAVVAGGIAGVAALIGGILLFHRRWTDPRIAKNSSFWDNAILLMLIAQLVLGLGTIFVSLGHLDGHEMVKFMSW
AQGIFTFDGAAAGYVADVAIVFKLHLILGLLIIAVFPFTRLVHILSGLAAPFRYLFGRTGYQVVRSRRKVALPKTVGAGS
QGRKSPMPERKGAAAPDAMPTPAE

Sequences:

>Translated_264_residues
MNQFLFGTFPYIALAICIIGSIARYERDPFTWKSSSSQMLRRRQLIIGSVLFHVGVLVIFFGHLVGLLTPIWIFDAIGIS
HGAKQLLAVVAGGIAGVAALIGGILLFHRRWTDPRIAKNSSFWDNAILLMLIAQLVLGLGTIFVSLGHLDGHEMVKFMSW
AQGIFTFDGAAAGYVADVAIVFKLHLILGLLIIAVFPFTRLVHILSGLAAPFRYLFGRTGYQVVRSRRKVALPKTVGAGS
QGRKSPMPERKGAAAPDAMPTPAE
>Mature_264_residues
MNQFLFGTFPYIALAICIIGSIARYERDPFTWKSSSSQMLRRRQLIIGSVLFHVGVLVIFFGHLVGLLTPIWIFDAIGIS
HGAKQLLAVVAGGIAGVAALIGGILLFHRRWTDPRIAKNSSFWDNAILLMLIAQLVLGLGTIFVSLGHLDGHEMVKFMSW
AQGIFTFDGAAAGYVADVAIVFKLHLILGLLIIAVFPFTRLVHILSGLAAPFRYLFGRTGYQVVRSRRKVALPKTVGAGS
QGRKSPMPERKGAAAPDAMPTPAE

Specific function: This is a second nitrate reductase enzyme which can substitute for the NRA enzyme and allows E.coli to use nitrate as an electron acceptor during anaerobic growth. The gamma chain is a membrane-embedded heme-iron unit resembling cytochrome b, which transf

COG id: COG2181

COG function: function code C; Nitrate reductase gamma subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787738, Length=228, Percent_Identity=48.6842105263158, Blast_Score=199, Evalue=1e-52,
Organism=Escherichia coli, GI1787480, Length=228, Percent_Identity=45.1754385964912, Blast_Score=191, Evalue=4e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003816 [H]

Pfam domain/function: PF02665 Nitrate_red_gam [H]

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 28712; Mature: 28712

Theoretical pI: Translated: 11.08; Mature: 11.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure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CCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]