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The map label for this gene is narK [H]

Identifier: 110679686

GI number: 110679686

Start: 2324450

End: 2325850

Strand: Direct

Name: narK [H]

Synonym: RD1_2441

Alternate gene names: 110679686

Gene position: 2324450-2325850 (Clockwise)

Preceding gene: 110679685

Following gene: 110679687

Centisome position: 56.24

GC content: 58.46

Gene sequence:

>1401_bases
ATGGCGATTACAGACACCTCAAATGGCAGCAGCCGTCACGTGTTGACGGACTGGCGCCCCGAAGACCCCCAGTTCTGGGA
GGAAAAGGGCAAGGCCATCGCAACCCGCAACCTTTGGATCTCGATCCCGAACCTGCTCATGGCCTTCTCGGTCTGGATGG
TTTGGTCGGTCGTTGTGGCCAAGATGCCTGCCATTGGCTTTGATTTCTCGGTTTCAGAGCGTTTCTGGCTGGCGGCTTTG
CCCGGCCTGTCGGGCGCAACACTGCGGATTTTCTACAGCTTCGTTATCCCGGTCTTTGGCGGCCGTCGGTGGACGGCCAT
CTCGACCGCTTCGCTTTTGCTGCCCGCACTCGGTATCGGCTTCGCGGTACAGAACCCCGAAACGTCATACTTCACATTCC
TGATCCTTGCCCTGATGTGCGGCTTCGGCGGCGGCAACTTCGCCTCTTCGATGGCCAACATCGCCTATTTCTATCCCAAG
AAAGTCAAAGGCCAGGCGCTGGCAATGAATGCGGGCCTGGGCAACCTTGGCGTCTCCGTGATGCAGTTTCTCGTGCCTGT
CGTCATCACCATGGGTGTTTTCGGCGCCGTGGGTGGTGAGCCGCAAACCCTGTCCGATGGCGGTCAACTCTTCATTCAGA
ATGCTGGCTTCATCTGGGTGCCGTTCCTGGCCATTGGTGCCATCGCCGCGTGGTTCGGCATGAATGACATCGCTGACGCA
AAGGCCTCTGTGGGCGAGCAGATGTCCATCCTGGGCCGCTACCACAACTGGGTGATGTGTGTGCTTTATGTTGGCACATT
CGGCTCCTTCATTGGCTATGCTGCGGGTTTCCCGCTTCTGATGAAGACACAGTTTCCAGAAGTTGATGTGCTGAAATACG
CCTTCCTCGGCCCGCTTGTGGGAGCGCTGAGCCGCTCTGCCACGGGCTGGATTTCCGACAAGTTCGGCGGTGGGAAAGTG
ACTTTCTGGGTGTTCCTCGGCATGATCGTTGCGGTTTACGGCGTGATCACCTTCTTGCCATCGACACCTGAAGCGGCTGA
CGGCAGTTTCTGGGGCTTCTTTGCCTGCTTCATGGCGCTCTTCTTCCTGACCGGTGTGGGCAATGCCTCCACCTTCCAGA
TGATCCCGTCGATCATGAAACAGGAAATCCCGCGTCTCATGCCGAACCTCGAGGCCACCAACATGCAGCGCACCATCGAG
CGCGAGTCTGCTGCCATCATTGCCTTTACGTCGGCCATCGCAGCTTACGGCGCCTTCTTCATCCCGAAGAGCTACGGCAC
CTCGATCGCCATAACCGGCAGTCCGCACGGCGCGCTCTGGGCCTTTCTCGGGTTCTATGCGGTCTGCACCGTAATCACCT
GGGTCTACTACAGCCGCAAGAACGCACCTGTTCCCTGCTGA

Upstream 100 bases:

>100_bases
GTCCAAACGGCAGCCAGCAACCGGCTGAATGAACGCATGGCCGGCCGGGCAAGGTGGCCCGGCCGGCCCCCCGAAACCGC
CGACAGGGGAGGATTTACCC

Downstream 100 bases:

>100_bases
CCCACTGACCAAGCTCTGCGCCGGATCGCCCGGCGCAGAGGTAGCCAAAAGGAGAAAATTCCATGAGCCACCTGCTCGAC
AGATTGAACTTCCTCCAGTC

Product: nitrite extrusion protein

Products: NA

Alternate protein names: Nitrite facilitator 1 [H]

Number of amino acids: Translated: 466; Mature: 465

Protein sequence:

>466_residues
MAITDTSNGSSRHVLTDWRPEDPQFWEEKGKAIATRNLWISIPNLLMAFSVWMVWSVVVAKMPAIGFDFSVSERFWLAAL
PGLSGATLRIFYSFVIPVFGGRRWTAISTASLLLPALGIGFAVQNPETSYFTFLILALMCGFGGGNFASSMANIAYFYPK
KVKGQALAMNAGLGNLGVSVMQFLVPVVITMGVFGAVGGEPQTLSDGGQLFIQNAGFIWVPFLAIGAIAAWFGMNDIADA
KASVGEQMSILGRYHNWVMCVLYVGTFGSFIGYAAGFPLLMKTQFPEVDVLKYAFLGPLVGALSRSATGWISDKFGGGKV
TFWVFLGMIVAVYGVITFLPSTPEAADGSFWGFFACFMALFFLTGVGNASTFQMIPSIMKQEIPRLMPNLEATNMQRTIE
RESAAIIAFTSAIAAYGAFFIPKSYGTSIAITGSPHGALWAFLGFYAVCTVITWVYYSRKNAPVPC

Sequences:

>Translated_466_residues
MAITDTSNGSSRHVLTDWRPEDPQFWEEKGKAIATRNLWISIPNLLMAFSVWMVWSVVVAKMPAIGFDFSVSERFWLAAL
PGLSGATLRIFYSFVIPVFGGRRWTAISTASLLLPALGIGFAVQNPETSYFTFLILALMCGFGGGNFASSMANIAYFYPK
KVKGQALAMNAGLGNLGVSVMQFLVPVVITMGVFGAVGGEPQTLSDGGQLFIQNAGFIWVPFLAIGAIAAWFGMNDIADA
KASVGEQMSILGRYHNWVMCVLYVGTFGSFIGYAAGFPLLMKTQFPEVDVLKYAFLGPLVGALSRSATGWISDKFGGGKV
TFWVFLGMIVAVYGVITFLPSTPEAADGSFWGFFACFMALFFLTGVGNASTFQMIPSIMKQEIPRLMPNLEATNMQRTIE
RESAAIIAFTSAIAAYGAFFIPKSYGTSIAITGSPHGALWAFLGFYAVCTVITWVYYSRKNAPVPC
>Mature_465_residues
AITDTSNGSSRHVLTDWRPEDPQFWEEKGKAIATRNLWISIPNLLMAFSVWMVWSVVVAKMPAIGFDFSVSERFWLAALP
GLSGATLRIFYSFVIPVFGGRRWTAISTASLLLPALGIGFAVQNPETSYFTFLILALMCGFGGGNFASSMANIAYFYPKK
VKGQALAMNAGLGNLGVSVMQFLVPVVITMGVFGAVGGEPQTLSDGGQLFIQNAGFIWVPFLAIGAIAAWFGMNDIADAK
ASVGEQMSILGRYHNWVMCVLYVGTFGSFIGYAAGFPLLMKTQFPEVDVLKYAFLGPLVGALSRSATGWISDKFGGGKVT
FWVFLGMIVAVYGVITFLPSTPEAADGSFWGFFACFMALFFLTGVGNASTFQMIPSIMKQEIPRLMPNLEATNMQRTIER
ESAAIIAFTSAIAAYGAFFIPKSYGTSIAITGSPHGALWAFLGFYAVCTVITWVYYSRKNAPVPC

Specific function: Involved in excretion of nitrite produced by the dissimilatory reduction of nitrate [H]

COG id: COG2223

COG function: function code P; Nitrate/nitrite transporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Nitrate/nitrite porter (TC 2.A.1.8) family [H]

Homologues:

Organism=Escherichia coli, GI1787475, Length=464, Percent_Identity=50.4310344827586, Blast_Score=458, Evalue=1e-130,
Organism=Escherichia coli, GI1787743, Length=465, Percent_Identity=50.9677419354839, Blast_Score=426, Evalue=1e-120,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004737 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 50452; Mature: 50321

Theoretical pI: Translated: 8.65; Mature: 8.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAITDTSNGSSRHVLTDWRPEDPQFWEEKGKAIATRNLWISIPNLLMAFSVWMVWSVVVA
CCCCCCCCCCCCEEEECCCCCCCHHHHHCCCEEEECHHEEEHHHHHHHHHHHHHHHHHHH
KMPAIGFDFSVSERFWLAALPGLSGATLRIFYSFVIPVFGGRRWTAISTASLLLPALGIG
HCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHCC
FAVQNPETSYFTFLILALMCGFGGGNFASSMANIAYFYPKKVKGQALAMNAGLGNLGVSV
EEECCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCEEEEECCCCHHHHHH
MQFLVPVVITMGVFGAVGGEPQTLSDGGQLFIQNAGFIWVPFLAIGAIAAWFGMNDIADA
HHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCHHHHH
KASVGEQMSILGRYHNWVMCVLYVGTFGSFIGYAAGFPLLMKTQFPEVDVLKYAFLGPLV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHH
GALSRSATGWISDKFGGGKVTFWVFLGMIVAVYGVITFLPSTPEAADGSFWGFFACFMAL
HHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHH
FFLTGVGNASTFQMIPSIMKQEIPRLMPNLEATNMQRTIERESAAIIAFTSAIAAYGAFF
HHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHC
IPKSYGTSIAITGSPHGALWAFLGFYAVCTVITWVYYSRKNAPVPC
CCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
AITDTSNGSSRHVLTDWRPEDPQFWEEKGKAIATRNLWISIPNLLMAFSVWMVWSVVVA
CCCCCCCCCCCEEEECCCCCCCHHHHHCCCEEEECHHEEEHHHHHHHHHHHHHHHHHHH
KMPAIGFDFSVSERFWLAALPGLSGATLRIFYSFVIPVFGGRRWTAISTASLLLPALGIG
HCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHCC
FAVQNPETSYFTFLILALMCGFGGGNFASSMANIAYFYPKKVKGQALAMNAGLGNLGVSV
EEECCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCEEEEECCCCHHHHHH
MQFLVPVVITMGVFGAVGGEPQTLSDGGQLFIQNAGFIWVPFLAIGAIAAWFGMNDIADA
HHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCHHHHH
KASVGEQMSILGRYHNWVMCVLYVGTFGSFIGYAAGFPLLMKTQFPEVDVLKYAFLGPLV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHH
GALSRSATGWISDKFGGGKVTFWVFLGMIVAVYGVITFLPSTPEAADGSFWGFFACFMAL
HHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHH
FFLTGVGNASTFQMIPSIMKQEIPRLMPNLEATNMQRTIERESAAIIAFTSAIAAYGAFF
HHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHC
IPKSYGTSIAITGSPHGALWAFLGFYAVCTVITWVYYSRKNAPVPC
CCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2668029; 8905232; 9278503; 2657652 [H]