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The map label for this gene is hydA [C]

Identifier: 110679653

GI number: 110679653

Start: 2293087

End: 2294484

Strand: Reverse

Name: hydA [C]

Synonym: RD1_2404

Alternate gene names: 110679653

Gene position: 2294484-2293087 (Counterclockwise)

Preceding gene: 110679654

Following gene: 110679652

Centisome position: 55.51

GC content: 62.59

Gene sequence:

>1398_bases
ATGACCCATGACACCGTCATCACCGGGGCCCGCGTCGTTGCTGCGAGCGGAACCACAATGTGCGACATCGGGATCACGCA
AGGACAGATCGCAACCCTTGGCAGCGCATTGACGGGCACGGAGGTGATCGACGCCACTGGCCTGATTGCCCTGCCCGGCG
GGATCGACAGCCATGTGCATATCTCGCAGCCCTCAGGCCCGGGGATTGAGATGGCCGATGATTTTATCTCGGGCACCCGC
GCGGCAGCTTGCGGCGGAAACACGACCATCCTGCCGTTTTGCCTGCCGGAGGACGGCCAAACCCTGCGCGAGGCGGTGGC
GGCCTACCGCAAGAAGGCAGAAGGCAACTGCATGACCGACGTGGCCTTTCACCTGATCGTCAAACAAACCGACCCGGTTA
CGCTGGGTCAGGACCTGCCCGCGCTGATCCGGGCCGGCTATACCAGCTTCAAGGTCTTCATGACCTACGCGGGCCTCGCC
CTGTCGGACCGCCAGATCCTCGACGTCATGGCCGTGGCAAAGGAACAGAATGCCATGGTGATGGTTCACGCCGAAAACGA
AGATGCCATCGAATTCCTGCGCGACCGGGCCGAGCGGGAAGGCGAAACCGGGCCCTACCATCATGCAACGACGCGGCCTA
TCCCGGTAGAGCGTGAAGCGACCCATCGCGCCATATCACTGGCCGAAATCGCGGGCGTGCCGCTGACCATCGTGCACGTC
TCCAACGGCGAGGCGACCGAGGAGATCGTGCGCGCCCGCGCGCGGGGACTGACGGTTTTTGCCGAAACCTGCCCGCAGTA
TATCACGCTGACTGCGTCTGATCTGGATCGCGACGGGTTTGACGGGGCGAAATACGTCTGCTCTCCCCCGCCGCGCACTG
CGGATGAATGGCCGAAGATATGGGCCGGCATCGAACAGGGCACATTTGATCTGTTTTCCTCAGACCACTGTCCGTTTCGC
TTCAATGATACGGCGGGCAAGGATGCTCCCGGCGCCCGCACGAGTTTTCGCAATATCCCAAACGGCATTCCGGGGGTCGA
GACACGCCTGCCGATCCTCTTTTCCGAAGGCGTGGTGGCAGGGCGTCTGTCGCTTGAACGCTTTGCTGCGATCACCGCCA
CCAATCACGCCCGTCTCTACGGGCTTTATCCGCGCAAGGGGGCGATCATGGTCGGTGCGGATGCCGACATAACGCTGTGG
GATCCCACAGTGACCAAGCCCATTCGCCAGCACGATCTGCATCACGGCTCTGATTACACACCATGGGAGGGATTCGAGGT
GACAGGCTGGCCTGTGCGCACCATCCTGCGCGGCAAATCCGTCATGCAGGATGGCCACCCCGTGGGTCCACCAATGGGCG
AACATCTGGAACGCGGGGCACCGGACGTGCAGCTCTGA

Upstream 100 bases:

>100_bases
GCCTTGCGCCCAGCTACGCCAATCGCACCAACCGCCCCTTGCACAAGACCTCAACCGGGTTGAGCACAGAGCTGACTGCC
GTCATCGCAGGAGAAAAAAC

Downstream 100 bases:

>100_bases
GCATGACGACAAAAAGTAATTTTGCAACAACACACATGTGACGTGCAAACAAGCGGAGGTGCCTGCACTCGCTTGATCCC
ATTGTCCGCAATCGCGTTTT

Product: dihydropyrimidinase

Products: 3-ureidopropanoate

Alternate protein names: NA

Number of amino acids: Translated: 465; Mature: 464

Protein sequence:

>465_residues
MTHDTVITGARVVAASGTTMCDIGITQGQIATLGSALTGTEVIDATGLIALPGGIDSHVHISQPSGPGIEMADDFISGTR
AAACGGNTTILPFCLPEDGQTLREAVAAYRKKAEGNCMTDVAFHLIVKQTDPVTLGQDLPALIRAGYTSFKVFMTYAGLA
LSDRQILDVMAVAKEQNAMVMVHAENEDAIEFLRDRAEREGETGPYHHATTRPIPVEREATHRAISLAEIAGVPLTIVHV
SNGEATEEIVRARARGLTVFAETCPQYITLTASDLDRDGFDGAKYVCSPPPRTADEWPKIWAGIEQGTFDLFSSDHCPFR
FNDTAGKDAPGARTSFRNIPNGIPGVETRLPILFSEGVVAGRLSLERFAAITATNHARLYGLYPRKGAIMVGADADITLW
DPTVTKPIRQHDLHHGSDYTPWEGFEVTGWPVRTILRGKSVMQDGHPVGPPMGEHLERGAPDVQL

Sequences:

>Translated_465_residues
MTHDTVITGARVVAASGTTMCDIGITQGQIATLGSALTGTEVIDATGLIALPGGIDSHVHISQPSGPGIEMADDFISGTR
AAACGGNTTILPFCLPEDGQTLREAVAAYRKKAEGNCMTDVAFHLIVKQTDPVTLGQDLPALIRAGYTSFKVFMTYAGLA
LSDRQILDVMAVAKEQNAMVMVHAENEDAIEFLRDRAEREGETGPYHHATTRPIPVEREATHRAISLAEIAGVPLTIVHV
SNGEATEEIVRARARGLTVFAETCPQYITLTASDLDRDGFDGAKYVCSPPPRTADEWPKIWAGIEQGTFDLFSSDHCPFR
FNDTAGKDAPGARTSFRNIPNGIPGVETRLPILFSEGVVAGRLSLERFAAITATNHARLYGLYPRKGAIMVGADADITLW
DPTVTKPIRQHDLHHGSDYTPWEGFEVTGWPVRTILRGKSVMQDGHPVGPPMGEHLERGAPDVQL
>Mature_464_residues
THDTVITGARVVAASGTTMCDIGITQGQIATLGSALTGTEVIDATGLIALPGGIDSHVHISQPSGPGIEMADDFISGTRA
AACGGNTTILPFCLPEDGQTLREAVAAYRKKAEGNCMTDVAFHLIVKQTDPVTLGQDLPALIRAGYTSFKVFMTYAGLAL
SDRQILDVMAVAKEQNAMVMVHAENEDAIEFLRDRAEREGETGPYHHATTRPIPVEREATHRAISLAEIAGVPLTIVHVS
NGEATEEIVRARARGLTVFAETCPQYITLTASDLDRDGFDGAKYVCSPPPRTADEWPKIWAGIEQGTFDLFSSDHCPFRF
NDTAGKDAPGARTSFRNIPNGIPGVETRLPILFSEGVVAGRLSLERFAAITATNHARLYGLYPRKGAIMVGADADITLWD
PTVTKPIRQHDLHHGSDYTPWEGFEVTGWPVRTILRGKSVMQDGHPVGPPMGEHLERGAPDVQL

Specific function: Catalyzes the stereospecific hydrolysis of the cyclic amide bond of D-hydantoin. Has no activity on dihydropyrimidines [H]

COG id: COG0044

COG function: function code F; Dihydroorotase and related cyclic amidohydrolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DHOase family. Hydantoinase/dihydropyrimidinase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4503379, Length=452, Percent_Identity=33.6283185840708, Blast_Score=246, Evalue=3e-65,
Organism=Homo sapiens, GI4503375, Length=453, Percent_Identity=35.3200883002208, Blast_Score=244, Evalue=2e-64,
Organism=Homo sapiens, GI190194363, Length=450, Percent_Identity=33.1111111111111, Blast_Score=243, Evalue=3e-64,
Organism=Homo sapiens, GI19923821, Length=447, Percent_Identity=35.5704697986577, Blast_Score=242, Evalue=6e-64,
Organism=Homo sapiens, GI4503051, Length=468, Percent_Identity=33.5470085470085, Blast_Score=240, Evalue=3e-63,
Organism=Homo sapiens, GI62422571, Length=468, Percent_Identity=33.5470085470085, Blast_Score=238, Evalue=8e-63,
Organism=Homo sapiens, GI4503377, Length=451, Percent_Identity=33.7028824833703, Blast_Score=236, Evalue=3e-62,
Organism=Homo sapiens, GI18105007, Length=431, Percent_Identity=26.4501160092807, Blast_Score=102, Evalue=8e-22,
Organism=Escherichia coli, GI87082175, Length=446, Percent_Identity=38.7892376681614, Blast_Score=312, Evalue=3e-86,
Organism=Escherichia coli, GI1786722, Length=465, Percent_Identity=31.3978494623656, Blast_Score=196, Evalue=2e-51,
Organism=Caenorhabditis elegans, GI17539558, Length=453, Percent_Identity=33.5540838852097, Blast_Score=230, Evalue=1e-60,
Organism=Caenorhabditis elegans, GI71989490, Length=459, Percent_Identity=34.4226579520697, Blast_Score=225, Evalue=5e-59,
Organism=Caenorhabditis elegans, GI86575075, Length=464, Percent_Identity=26.5086206896552, Blast_Score=157, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI193204318, Length=414, Percent_Identity=23.6714975845411, Blast_Score=92, Evalue=7e-19,
Organism=Saccharomyces cerevisiae, GI6322218, Length=421, Percent_Identity=29.2161520190024, Blast_Score=137, Evalue=4e-33,
Organism=Drosophila melanogaster, GI221377917, Length=451, Percent_Identity=36.3636363636364, Blast_Score=252, Evalue=3e-67,
Organism=Drosophila melanogaster, GI17137462, Length=448, Percent_Identity=35.0446428571429, Blast_Score=246, Evalue=2e-65,
Organism=Drosophila melanogaster, GI24644287, Length=296, Percent_Identity=37.5, Blast_Score=159, Evalue=3e-39,
Organism=Drosophila melanogaster, GI24644289, Length=229, Percent_Identity=37.5545851528384, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24642586, Length=427, Percent_Identity=25.2927400468384, Blast_Score=108, Evalue=6e-24,
Organism=Drosophila melanogaster, GI18859883, Length=441, Percent_Identity=27.437641723356, Blast_Score=91, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006680
- InterPro:   IPR011778
- InterPro:   IPR011059 [H]

Pfam domain/function: PF01979 Amidohydro_1 [H]

EC number: 3.5.2.2

Molecular weight: Translated: 50041; Mature: 49910

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: PS00237 G_PROTEIN_RECEP_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHDTVITGARVVAASGTTMCDIGITQGQIATLGSALTGTEVIDATGLIALPGGIDSHVH
CCCCEEEECCEEEEECCCEEEEECCCCCHHHHHCCCCCCCHHCCCCCEEEECCCCCCCEE
ISQPSGPGIEMADDFISGTRAAACGGNTTILPFCLPEDGQTLREAVAAYRKKAEGNCMTD
EECCCCCCCCHHHHHHCCCCEEECCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCCHHH
VAFHLIVKQTDPVTLGQDLPALIRAGYTSFKVFMTYAGLALSDRQILDVMAVAKEQNAMV
EEEEEEEECCCCEECCCCCHHHHHCCCHHHEEEEHHHCCEECCHHHHHHHHHHCCCCCEE
MVHAENEDAIEFLRDRAEREGETGPYHHATTRPIPVEREATHRAISLAEIAGVPLTIVHV
EEECCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEE
SNGEATEEIVRARARGLTVFAETCPQYITLTASDLDRDGFDGAKYVCSPPPRTADEWPKI
CCCCHHHHHHHHHHCCCEEEECCCCCEEEEECCCCCCCCCCCCCEEECCCCCCCHHHHHH
WAGIEQGTFDLFSSDHCPFRFNDTAGKDAPGARTSFRNIPNGIPGVETRLPILFSEGVVA
HHCCCCCEEECCCCCCCCEEECCCCCCCCCCCCHHHHCCCCCCCCCCCCCCEEEECCEEE
GRLSLERFAAITATNHARLYGLYPRKGAIMVGADADITLWDPTVTKPIRQHDLHHGSDYT
EHHHHHHHEEEEECCCEEEEEECCCCCEEEEECCCCEEEECCHHCCHHHHHCCCCCCCCC
PWEGFEVTGWPVRTILRGKSVMQDGHPVGPPMGEHLERGAPDVQL
CCCCEEECCCHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCCCCCC
>Mature Secondary Structure 
THDTVITGARVVAASGTTMCDIGITQGQIATLGSALTGTEVIDATGLIALPGGIDSHVH
CCCEEEECCEEEEECCCEEEEECCCCCHHHHHCCCCCCCHHCCCCCEEEECCCCCCCEE
ISQPSGPGIEMADDFISGTRAAACGGNTTILPFCLPEDGQTLREAVAAYRKKAEGNCMTD
EECCCCCCCCHHHHHHCCCCEEECCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCCHHH
VAFHLIVKQTDPVTLGQDLPALIRAGYTSFKVFMTYAGLALSDRQILDVMAVAKEQNAMV
EEEEEEEECCCCEECCCCCHHHHHCCCHHHEEEEHHHCCEECCHHHHHHHHHHCCCCCEE
MVHAENEDAIEFLRDRAEREGETGPYHHATTRPIPVEREATHRAISLAEIAGVPLTIVHV
EEECCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEE
SNGEATEEIVRARARGLTVFAETCPQYITLTASDLDRDGFDGAKYVCSPPPRTADEWPKI
CCCCHHHHHHHHHHCCCEEEECCCCCEEEEECCCCCCCCCCCCCEEECCCCCCCHHHHHH
WAGIEQGTFDLFSSDHCPFRFNDTAGKDAPGARTSFRNIPNGIPGVETRLPILFSEGVVA
HHCCCCCEEECCCCCCCCEEECCCCCCCCCCCCHHHHCCCCCCCCCCCCCCEEEECCEEE
GRLSLERFAAITATNHARLYGLYPRKGAIMVGADADITLWDPTVTKPIRQHDLHHGSDYT
EHHHHHHHEEEEECCCEEEEEECCCCCEEEEECCCCEEEECCHHCCHHHHHCCCCCCCCC
PWEGFEVTGWPVRTILRGKSVMQDGHPVGPPMGEHLERGAPDVQL
CCCCEEECCCHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 5,6-dihydrouracil; H2O

Specific reaction: 5,6-dihydrouracil + H2O = 3-ureidopropanoate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7765480 [H]